psiCHECK-2-h1.470046

LOCUS       psiCHECK-2-h1.470046             6609 bp DNA     circular SYN 22-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    psiCHECK-2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..1629
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    1681..2008
                     /label=-3
     polyA_signal    2024..2072
                     /note="synthetic polyadenylation signal"
     promoter        2081..2832
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      2862..2871
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2868..4520
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             3827..3835
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    4564..4685
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4818..4922
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4923..5783
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5954..6542
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag
      721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg
      781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt
      841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc
      901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat
      961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac
     1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac
     1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg
     1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa
     1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag
     1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag
     1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc
     1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat
     1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga
     1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag
     1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac
     1621 gagcagtaat tctaggcgat cgctcgagcc cgggaattcg tttaaaccta gagcggccgc
     1681 ggccaaactt gaacgagaat ggtcagggaa aggctcggcg aggatatgat agtgagaaat
     1741 aagtaggaga ccagtaggaa aacaagggaa gggaagttgt accaggcaga tgaagcagca
     1801 cgttcaaagg cttggaggca caagaacatg gtgcaatcaa gaacatgcaa gcatccgctc
     1861 tccttgttct atttcctgca accctatatg aatttgcttt catgtagtaa gatttctgtt
     1921 gtgtaagctt aatctccttt gatatttctt tgataatact ttcatcattt ttcttcttat
     1981 gtccatagac ttcatcctct actctggcgc ggccgctggc cgcaataaaa tatctttatt
     2041 ttcattacat ctgtgtgttg gttttttgtg tgaggatcta aatgagtctt cggacctcgc
     2101 gggggccgct taagcggtgg ttagggtttg tctgacgcgg ggggaggggg aaggaacgaa
     2161 acactctcat tcggaggcgg ctcggggttt ggtcttggtg gccacgggca cgcagaagag
     2221 cgccgcgatc ctcttaagca cccccccgcc ctccgtggag gcgggggttt ggtcggcggg
     2281 tggtaactgg cgggccgctg actcgggcgg gtcgcgcgcc ccagagtgtg accttttcgg
     2341 tctgctcgca gacccccggg cggcgccgcc gcggcggcga cgggctcgct gggtcctagg
     2401 ctccatgggg accgtatacg tggacaggct ctggagcatc cgcacgactg cggtgatatt
     2461 accggagacc ttctgcggga cgagccgggt cacgcggctg acgcggagcg tccgttgggc
     2521 gacaaacacc aggacggggc acaggtacac tatcttgtca cccggaggcg cgagggactg
     2581 caggagcttc agggagtggc gcagctgctt catccccgtg gcccgttgct cgcgtttgct
     2641 ggcggtgtcc ccggaagaaa tatatttgca tgtctttagt tctatgatga cacaaacccc
     2701 gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc
     2761 caggtccact tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag
     2821 cgacccgctt aaaagcttgg cattccggta ctgttggtaa agccaccatg gccgatgcta
     2881 agaacattaa gaagggccct gctcccttct accctctgga ggatggcacc gctggcgagc
     2941 agctgcacaa ggccatgaag aggtatgccc tggtgcctgg caccattgcc ttcaccgatg
     3001 cccacattga ggtggacatc acctatgccg agtacttcga gatgtctgtg cgcctggccg
     3061 aggccatgaa gaggtacggc ctgaacacca accaccgcat cgtggtgtgc tctgagaact
     3121 ctctgcagtt cttcatgcca gtgctgggcg ccctgttcat cggagtggcc gtggcccctg
     3181 ctaacgacat ttacaacgag cgcgagctgc tgaacagcat gggcatttct cagcctaccg
     3241 tggtgttcgt gtctaagaag ggcctgcaga agatcctgaa cgtgcagaag aagctgccta
     3301 tcatccagaa gatcatcatc atggactcta agaccgacta ccagggcttc cagagcatgt
     3361 acacattcgt gacatctcat ctgcctcctg gcttcaacga gtacgacttc gtgccagagt
     3421 ctttcgacag ggacaaaacc attgccctga tcatgaacag ctctgggtct accggcctgc
     3481 ctaagggcgt ggccctgcct catcgcaccg cctgtgtgcg cttctctcac gcccgcgacc
     3541 ctattttcgg caaccagatc atccccgaca ccgctattct gagcgtggtg ccattccacc
     3601 acggcttcgg catgttcacc accctgggct acctgatttg cggctttcgg gtggtgctga
     3661 tgtaccgctt cgaggaggag ctgttcctgc gcagcctgca agactacaaa attcagtctg
     3721 ccctgctggt gccaaccctg ttcagcttct tcgctaagag caccctgatc gacaagtacg
     3781 acctgtctaa cctgcacgag attgcctctg gcggcgcccc actgtctaag gaggtgggcg
     3841 aagccgtggc caagcgcttt catctgccag gcatccgcca gggctacggc ctgaccgaga
     3901 caaccagcgc cattctgatt accccagagg gcgacgacaa gcctggcgcc gtgggcaagg
     3961 tggtgccatt cttcgaggcc aaggtggtgg acctggacac cggcaagacc ctgggagtga
     4021 accagcgcgg cgagctgtgt gtgcgcggcc ctatgattat gtccggctac gtgaataacc
     4081 ctgaggccac aaacgccctg atcgacaagg acggctggct gcactctggc gacattgcct
     4141 actgggacga ggacgagcac ttcttcatcg tggaccgcct gaagtctctg atcaagtaca
     4201 agggctacca ggtggcccca gccgagctgg agtctatcct gctgcagcac cctaacattt
     4261 tcgacgccgg agtggccggc ctgcccgacg acgatgccgg cgagctgcct gccgccgtcg
     4321 tcgtgctgga acacggcaag accatgaccg agaaggagat cgtggactat gtggccagcc
     4381 aggtgacaac cgccaagaag ctgcgcggcg gagtggtgtt cgtggacgag gtgcccaagg
     4441 gcctgaccgg caagctggac gcccgcaaga tccgcgagat cctgatcaag gctaagaaag
     4501 gcggcaagat cgccgtgtaa taattctaga gtcggggcgg ccggccgctt cgagcagaca
     4561 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     4621 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     4681 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     4741 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccaggtggca
     4801 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4861 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4921 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4981 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5041 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5101 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5161 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5221 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5281 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5341 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5401 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5461 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5521 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5581 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5641 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5701 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5761 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5821 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5881 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5941 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6001 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6061 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6121 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6181 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6241 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6301 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6361 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6421 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6481 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6541 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6601 tggctcgac
//