| 质粒名称: | pYK218 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000803 |
| 产品用途: | Mammalian PhCMV-driven constitutive NLS-MCP-2xANR expression vector (PhCMV-NLS-MCP-2xANR-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | NLS-MCP-2xANR |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK218
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK218载体质粒基本信息
pYK218质粒图谱载体图谱和pYK218载体序列质粒序列多克隆位点信息
pYK218质粒载体简介
pYK218质粒序列载体序列
LOCUS pYK218 6172 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6172)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 27, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6172
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..994
/label=oYK57-NLS-F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
misc_feature 905..964
/label=NLS2
primer_bind complement(943..967)
/label=oYK163_NLS_R
primer_bind complement(945..987)
/label=oYK57-NLS-R
primer_bind complement(945..984)
/label=oYK54-NLS-R
primer_bind complement(945..984)
/label=oYK167_NLS_R
primer_bind complement(945..969)
/label=oYK164_NLS_R
primer_bind 964..985
/label=oYK05-mcp-F
CDS 968..1354
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
VKAMQGLLKDGNPIPSAIAANSGIY"
primer_bind 968..994
/label=oYK57-mcp-mCherry-F
regulatory 968
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
primer_bind complement(1334..1384)
/label=oSCL093
primer_bind complement(1334..1354)
/label=Oscl32-2
primer_bind 1354..1374
/label=oLZ048 NSP3-F
misc_feature 1355..1389
/label=linker
primer_bind 1355..1384
/label=oSCL388
primer_bind 1355..1374
/label=oSCL1192
primer_bind 1355..1372
/label=oLZ147 NS3a F
primer_bind 1355..1371
/label=oYF0033
primer_bind complement(1365..1390)
/label=oSCL1119
primer_bind complement(1365..1384)
/label=oSCL075
primer_bind complement(1373..1410)
/label=oYK82_mcp_R
primer_bind complement(1373..1391)
/label=oYK05-mcp-R
primer_bind complement(1383..1409)
/label=oYK435_GS_R
CDS 1391..1465
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(1391..1410)
/label=oYK195_ANR_R
primer_bind 1391..1405
/label=oYK82_LinkANR_F
CDS 1466..1528
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1466..1481
/label=Primer 4
primer_bind complement(1505..1548)
/label=oYK195_ANR_R
primer_bind complement(1510..1528)
/label=oYK82_LinkANR_R
primer_bind 1513..1527
/label=Primer 3
CDS 1529..1603
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(1529..1548)
/label=oYK82_mcp_R
primer_bind 1529..1543
/label=oYK82_LinkANR_F
CDS 1604..1666
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1604..1619
/label=Primer 4
primer_bind complement(1643..1666)
/label=oYK195_ANR_R
primer_bind complement(1648..1689)
/label=oYK82_LinkANR_R
primer_bind 1651..1665
/label=Primer 3
terminator 1766..1993
/label=bGH PA
misc_feature 2039..2467
/label=f1 origin
misc_feature 2472..2815
/label=SV40 early promoter and origin
misc_feature 2661..2738
/label=SV40 ORI
CDS 2880..3671
/label=Kan/neoR
misc_feature 3845..3975
/label=SV40 early polyadenylation signal
primer_bind complement(4011..4031)
/label=M13-rev
misc_binding complement(4037..4059)
/label=LacO
promoter complement(4064..4093)
/label=lac
misc_feature complement(4358..5031)
/label=pUC origin
CDS complement(5179..5838)
/label=AmpR
promoter complement(6078..6106)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 caccatgccc aagaagaagc gcaaggtgga agctagcgct tccccgaaga aaaagcggaa
961 agtcatggct tcaaacttta ctcagttcgt gctcgtggac aatggtggga caggggatgt
1021 gacagtggct ccttctaatt tcgctaatgg ggtggcagag tggatcagct ccaactcacg
1081 gagccaggcc tacaaggtga catgcagcgt caggcagtct agtgcccaga agagaaagta
1141 taccatcaag gtggaggtcc ccaaagtggc tacccagaca gtgggcggag tcgaactgcc
1201 tgtcgccgct tggaggtcct acctgaacat ggagctcact atcccaattt tcgctaccaa
1261 ttctgactgt gaactcatcg tgaaggcaat gcaggggctc ctcaaagacg gtaatcctat
1321 cccttccgcc atcgccgcta actcaggtat ctacgccagc ggaagtggcg gaggaggcga
1381 cgtcggatcc ggctccggaa cgggcagcgg gaccggaagt ggcaccggaa ccacttccgg
1441 aaccggtact ggcgggagta ccggcggtga gctagacgaa ctcgtgtacc tgttggatgg
1501 ccccggttac gacccaatcc acagcgatgg ctccggaacg ggcagcggga ccggaagtgg
1561 caccggaacc acttccggaa ccggtactgg cgggagtacc ggcggtgagc tagacgaact
1621 cgtgtacctg ttggatggcc ccggttacga cccaatccac agcgattaag gatccactag
1681 tccagtgtgg tggaattctg cagatatcca gcacagtggc ggccgctcga gtctagaggg
1741 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
1801 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
1861 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
1921 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
1981 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
2041 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
2101 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
2161 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
2221 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
2281 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
2341 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
2401 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
2461 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
2521 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
2581 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
2641 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
2701 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
2761 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
2821 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
2881 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
2941 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
3001 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
3061 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
3121 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
3181 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
3241 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
3301 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
3361 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
3421 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
3481 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
3541 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
3601 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
3661 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
3721 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
3781 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
3841 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
3901 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
3961 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
4021 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
4081 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
4141 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
4201 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
4261 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
4321 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
4381 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
4441 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
4501 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
4561 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
4621 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
4681 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
4741 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
4801 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
4861 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
4921 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
4981 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
5041 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
5101 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
5161 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
5221 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
5281 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
5341 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
5401 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
5461 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
5521 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
5581 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
5641 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
5701 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
5761 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
5821 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
5881 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
5941 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
6001 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
6061 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
6121 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
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