P427-FBP_13

LOCUS       P427-FBP_13              22668 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  T-DNA encoding Fungal Bioluminescent Pathway Components, complete 
            pathway.
ACCESSION   .
VERSION     .
KEYWORDS    P427-FBP_13
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22668)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 22668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..22668
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(5..22)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5..21)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14..36)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    224..248
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1549..2178
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2607..3680
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3746..3940
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4176..4198)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4284..4424
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4339..4358
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4439..4456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4610..5198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4690..4709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5285..6079)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6504..6528
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6606..6780
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6837..7634)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6948..6967)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7558..7577
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7697..8373)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    8564..8585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8600..8630
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8638..8654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8643..8665
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8662..8678
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8662..8678
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      12532..12732
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     promoter        12769..13149
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     terminator      14421..14673
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     promoter        14785..15130
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    15149..15204
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     polyA_signal    20337..20511
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
       61 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      121 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg
      181 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
      241 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
      301 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
      361 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
      421 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
      481 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
      541 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
      601 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
      661 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
      721 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
      781 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
      841 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
      901 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
      961 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     1021 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     1081 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     1141 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     1201 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     1261 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     1321 cgggtacgtg ttcgagccgc ccgcgcacgg ctcaaccgtg cggctgcatg aaatcctggc
     1381 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     1441 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     1501 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     1561 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     1621 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     1681 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     1741 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     1801 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     1861 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     1921 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     1981 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     2041 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     2101 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     2161 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     2221 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     2281 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     2341 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     2401 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     2461 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     2521 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact
     2581 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
     2641 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
     2701 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
     2761 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
     2821 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
     2881 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
     2941 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
     3001 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
     3061 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
     3121 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
     3181 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
     3241 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
     3301 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
     3361 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
     3421 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
     3481 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
     3541 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
     3601 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
     3661 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
     3721 aaaaaggtcg aaaaggcctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
     3781 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
     3841 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
     3901 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
     3961 cccaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
     4021 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
     4081 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
     4141 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggaa
     4201 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     4261 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     4321 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     4381 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggccctct tccgcttcct
     4441 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4501 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4561 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4621 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4681 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4741 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4801 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4861 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4921 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4981 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5041 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5101 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5161 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5221 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
     5281 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
     5341 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
     5401 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
     5461 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
     5521 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
     5581 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
     5641 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
     5701 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
     5761 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
     5821 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
     5881 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
     5941 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
     6001 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
     6061 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
     6121 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
     6181 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
     6241 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
     6301 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
     6361 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
     6421 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
     6481 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
     6541 agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt
     6601 cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag
     6661 aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga
     6721 aaccctatag gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa
     6781 ctcgagcttg tcgatcgact ctagctagag gatcgatccg aaccccagag tcccgctcag
     6841 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     6901 taaagcacga ggaagcggtc agcccattcg ccgccaagtt cttcagcaat atcacgggta
     6961 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     7021 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg tgtcacgacg
     7081 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
     7141 ccctgatgtt cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     7201 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     7261 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     7321 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     7381 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     7441 gcctcgtcct ggagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     7501 cgcccctgcg ctgacagccg aaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     7561 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     7621 tgttcaatcc ccatggtcga tcgacagatc tgcgaaagct cgagagagat agatttgtag
     7681 agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg
     7741 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
     7801 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
     7861 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc
     7921 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga
     7981 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa
     8041 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga
     8101 gagtgtcgtg ctccaccatg ttcacatcaa tccacttgct ttgaagacgt ggttggaacg
     8161 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
     8221 gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct
     8281 tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg
     8341 tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta
     8401 tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc aagctgctct
     8461 agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     8521 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     8581 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     8641 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat tcgagctcgg
     8701 tacccgggga tcggcgcgcc ctatggagaa tatcattttt aagtaaaatt ttgaattcaa
     8761 atgttacgtg tattatttaa ttcatcaatt tgccttgtca tagcgagtac attacaaaca
     8821 tcacatatat ttgattgatt gtcaaaaaat atcaaaatat atatcaattt taagaggtat
     8881 aggtgtctaa tatgtactag ccctaattta aatatctaaa ttaattattc ggatgaatct
     8941 atataccatc tttttaatgg acacccaaaa tcacacatca aacatcatat acatgttgaa
     9001 aacatattat tgatatagct acatatatgt tttaatataa ataaaagacg agtcatatat
     9061 tcaaaaatta agaatcaaat aattttaatt tatttaatat tcaaaactta atactattta
     9121 aatttagata ttctaatttt aatacacgtc tgataaaata gatgaggact aaataaataa
     9181 tttgagacta tcttttcttt atttggcggc ccacaaataa tttagattct cgtaaccccc
     9241 tctttttctc tcactgaaaa agcacaatcc gtgtccaaac acaaagaagc actcgacacc
     9301 gtagatctcc attcagatca acggcttata ttcagttttc tccattcacg tggatcgaca
     9361 ttcttatccg tccgattatc aataaatttc ccaaaattta gcggccatga ttttaacccc
     9421 gcctcatttc aaaccgccca cgaaatcctc gacgcccaaa ttcaccaact ataaatagcc
     9481 accaccatcc ccttcatcaa tcatcaaatt tcataaccct agaatcatca cctttttcaa
     9541 atttcaatgg ctcctatctc ctccacctgg tccagattaa tcaggttcgt agctatagaa
     9601 acgtcactcg tgcatatagg cgaaccgatt gatgcaacta tggacgtagg gcttgctcgt
     9661 cgagaaggga aaactattca ggcctatgag attatcggat ctggcagcgc cctggacctt
     9721 tctgcacagg tgagtaagaa cgtgctcaca gtacgagaat tgttgatgcc actttctaga
     9781 gaagagataa agacggtcag atgcttaggc cttaactatc ctgtccacgc aacagaagcc
     9841 aatgttgctg taccgaagtt tccgaacctg ttctacaaac cagtcacaag tctcatcggg
     9901 cctgatggtc ttatcacgat ccctagtgta gtgcaaccgc ctaaggaaca tcagtcagat
     9961 tatgaagctg aacttgtgat tgtaatcgga aaggccgcca agaacgtgtc tgaggacgaa
    10021 gccttggatt atgtcttagg ctatacagct gctaacgata ttagtttccg aaaacaccag
    10081 ctcgcagtct cacagtggtc attctcaaaa ggatttggat ctctccttct cactatacgt
    10141 atggcccaga cacatagtgg aaacataaat aggttctcac gtgatcaaat ctttaatgtt
    10201 aagaaaacaa tcagcttttt gtctcaagga actaccctcg aacctgggtc tatcattttg
    10261 actggcaccc ctgatggggt cggcttcgtc aggaacccgc ccttatactt aaaagatggg
    10321 gatgaagtga tgacttggat aggttccggc attggtacgc ttgctaacac cgttcaagaa
    10381 gagaaaacat gctttgcaag cggtgggcat gagtaggctt ttatgttggt gatatggtgg
    10441 taaatgtagg gatttagttt acaattgcgt atgtctgtgt tggatatctg tagtgctgtt
    10501 cttatggctt agatcttgta atttctcatt acagtatcaa tgaatagata tcagtttcta
    10561 gtgatgacat tggttcgtct tttagctgtt gattaatttt tcttaattga ttcatcctat
    10621 tgcaattctt ctgaatttaa attgtatact gtgaaattaa gaaaattctt gaaattaatg
    10681 agaatttgag taatagcgct gttagagctc tgacggcgcg ccctatggag aattcatggg
    10741 gcattacaat taattagtaa tactaatttt aataacacta aatcatatta gtctctatag
    10801 gtataatata taactctgga gtcactatct gtatttggga tcaacagtta caaaggtacg
    10861 agtaaagtct ctgatccgac aaacaatccg tatatcctac cttcccgaaa tttcatgtat
    10921 aaaattatac taaaatatta tttttcttgt attatccgat tgaaaattat gcgaattcaa
    10981 cataaaactc taaaaaaaga aaataatgaa aattacaact taaaagagtg tctaagttgg
    11041 aatcatggta ttacataaag ttgaggacaa tataagcaag caaacaagta ctctagctat
    11101 caatttactt tggactacta tatgataaat atttcaaccc ccctcctccc ccaccaaaaa
    11161 aaaattaaaa taacaagttg aaggactcan aaaaaaaaaa aaaaaaaaac tcaaaaccaa
    11221 cctcaatcat acattcatat cctcttccta cccccatctt ggatgagata agattaacga
    11281 ggtgcttaca cgtgtcacct ctattgtggt gacttaaaaa aaattccaac ctttcatatg
    11341 tagatattaa gtaattgtat aatgttatca agaaccacat aacatatcaa aaaccttatc
    11401 atttcattat ataaaaggat agtggacatc aaaaggttca tattgaacca aaaaaagaga
    11461 gaagaagcaa aatggtgcag gacacttcaa gtgctagcac aagtccaatt ttaaccagat
    11521 ggtatataga cactcgacct ctcacggcaa gtaccgccgc acttccgctg ctggaaacgc
    11581 tgcaaccagc tgatcagatc tccgtccaaa aatattatca tctcaaggac aagcacatgt
    11641 cacttgcctc caaccttttg aagtatttat tcgttcatcg aaattgccga ataccatgga
    11701 gtagtattgt catatcaaga acgcccgacc cgcacagaag gccttgctat atacctcctt
    11761 caggttcaca agaggactcc ttcaaagacg gatatacggg aatcaacgta gaattcaacg
    11821 ttagtcacca agccagcatg gtggcaatcg caggtacggc tttcacacct aatagcggtg
    11881 gagatagcaa actcaaaccg gaggtcggaa tagatataac atgtgtaaat gagaggcagg
    11941 gcaggaatgg agaagaaaga tccttggaat ccctgaggca gtatatagat atattcagcg
    12001 aagtattcag caccgcagag atggctaata taaggcgact tgatggggtt tcctcaagtt
    12061 cccttagtgc agacagactc gtagattatg gataccgatt attctacacc tattgggcct
    12121 taaaagaggc ttatatcaag atgacaggtg aggccttatt ggctccttgg ttgagagagc
    12181 tggagttttc caacgtggtc gctccagccg ctgtcgccga aagcggggac tctgctggag
    12241 actttggcga accgtacact ggcgtgcgta caacattgta taagaacctc gtagaggacg
    12301 tacgaattga ggtagctgct ttgggaggag attatctgtt cgcaacagcc gctagaggtg
    12361 gtggtatagg cgcatcctca cgtcctggtg gtggcccaga tggctctgga attagatccc
    12421 aggacccttg gaggccgttc aagaaactcg atatagagcg tgacatacag ccatgtgcta
    12481 cgggcgtttg taattgcctg tcctcaggat aggcttgatc ccccgtcgac agctagctat
    12541 atcatcaatt tatgtattac acataatatc gcactcagtc tttcatctac ggcaatgtac
    12601 cagctgatat aatcagttat tgaaatattt ctgaatttaa acttgcatca ataaatttat
    12661 gtttttgctt ggactataat acctgacttg ttattttatc aataaatatt taaactatat
    12721 ttctttcaag atcgctgtta gagctcggac ggcgcgccct atggagattt ttcaaatcag
    12781 tgcgcaagac gtgacgtaag tatccgagtc agtttttatt tttctactaa tttggtcgtt
    12841 tatttcggcg tgtaggacat ggcaaccggg cctgaatttc gcgggtattc tgtttctatt
    12901 ccaacttttt cttgatccgc agccattaac gacttttgaa tagatacgct gacacgccaa
    12961 gcctcgctag tcaaaagtgt accaaacaac gctttacagc aagaacggaa tgcgcgtgac
    13021 gctcgcggtg acgccatttc gccttttcag aaatggataa atagccttgc ttcctattat
    13081 atcttcccaa attaccaata cattacacta gcatctgaat ttcataacca atctcgatac
    13141 accaaatcga atggcatcct ttgaaaattc actcagtgtc ttaatagttg gggctggctt
    13201 aggtggtttg gccgctgcta tagcactgcg aaggcaagga catgtagtca agatatacga
    13261 tagttcttca ttcaaggccg aattgggagc aggattagct gtaccaccca atactctccg
    13321 ttccctgcaa caactgggct gtaatacaga aaacctgaac ggagtagaca acctctgctt
    13381 tacggccatg ggatacgacg ggagtgtagg tatgatgaat aatatgaccg actaccgtga
    13441 ggcatacggc acttcctgga taatggtaca tcgagtggat ctccacaacg agctgatgcg
    13501 agtcgccttg gatccgggcg ggttagggcc tcctgccact cttcacctta accatcgtgt
    13561 tacgttctgc gatgtggatg cctgtacagt cacgtttacg aacgggacga cacagtctgc
    13621 tgacttgatt gtcggagctg atggtattag gtctacgatt cgaagattcg ttctggagga
    13681 ggacgtgact gttccagcta gcggaattgt cggctttcga tggttggtcc aggccgatgc
    13741 tctcgaccca tatcccgaac tggattggat tgtcaaaaaa ccacccttgg gagccaggtt
    13801 gatttctact ccacagaacc cgcagagtgg cgtaggactg gccgatagac gaacgatcat
    13861 tatttacgca tgcagaggtg ggaccatggt taatgtatta gccgtacacg acgacgaaag
    13921 agatcaaaac actgctgatt ggtccgttcc cgcatcaaag gatgatttat tcagagtgtt
    13981 tcacgattac catccaagat tccgacgact cttagagtta gcacaggaca ttaatttatg
    14041 gcagatgagg gtggtcccgg tcttgaaaaa atgggtgaac aagagggtat gcttgctggg
    14101 tgatgccgcc catgcttcac tgcccacact tggacaaggc ttcgggatgg ggttggagga
    14161 tgcagtcgct ttgggcacgt tgttgcctaa gggaacgacc gcatcacaga tcgagacaag
    14221 gctggccgtt tacgaacagc tgcgaaagga tcgagctgag tttgtcgccg cagagagcta
    14281 tgaggagcaa tatgtcccag agatgagagg gctgtacctt aggtccaaag aattgagaga
    14341 cagagtgatg ggttatgata ttaaggtaga gtcagagaag gttttggaaa cactgttgcg
    14401 atcatccaac tcagcttgag cttggactcc catgttggca aaggcaacca aacaaacaat
    14461 gaatgatccg ctcctgcata tggggcggtt tgagtatttc aactgccatt tgggctgaat
    14521 tgtagacatg ctcctgtcag aaattccgtg atcttactca atattcagta atctcggcca
    14581 atatcctaaa tgtgcgtggc tttatctgtc tttgtattgt ttcatcaatt catgtaacgt
    14641 ttgcttttct tatgaatttt caaataaatt atccgctgtt agagctcccg gggcgcgccc
    14701 tatggaggtc aacatggtgg agcacgacac tctggtctac tccaaaaatg tcaaagatac
    14761 agtctcagaa gatcaaaggg ctattgagac ttttcaacaa aggataattt cgggaaacct
    14821 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg
    14881 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atctctctgc
    14941 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagaggt
    15001 tccaaccacg tctacaaagc aagtggattg atgtgacatc tccactgacg taagggatga
    15061 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
    15121 ggagaggaca cgctactgta tttttacaac aattaccaac aacaacaaac aacaaacaac
    15181 attacaatta ctatttacaa ttacaatgaa tagtagtaaa aaccctccat ccactctgtt
    15241 agatgttttt ctggataccg ctaggaacct tgatacagca tccagaaatg tcctggagtg
    15301 cggagaacac aggtggagtt acagagagtt ggacacagtg tcatccgcat tggcccaaca
    15361 ccttcgatac actgtgggtc ttagccccac tgtagccgtg attagcgaga atcaccccta
    15421 catacttgca ttaatgctcg ccgtatggaa actcggcgga acgttcgcac cgatagatgt
    15481 tcattcccca gccgaacttg ttgctggaat gttgaatatt gtaagcccgt catgtcttgt
    15541 cattcctagc tctgatgtga caaatcagac attggcatgc gatcttaata taccggttgt
    15601 tgcattccac ccacatcaaa gtacaattcc cgaactcaac aagaagtatc tcacagattc
    15661 tcagatcagt cctgacctgc ccttccccga ccctaatcga cctgctttgt acttattcac
    15721 gtcatctgca acgtctaggt ccaatttgaa gtgcgtgcca cttacacaca cattcatatt
    15781 gcgaaactcc ttaagtaaac gagcatggtg caaaagaatg aggccagaga cagattttga
    15841 tggtattagg gtcctggggt gggccccctg gagccatgtc ctcgctcata tgcaggacat
    15901 tggccctctt acattattga atgccgggtg ctacgtattt gccacgaccc catctaccta
    15961 cccgacggag ctgaaggatg accgtgatgt tattagttgc gccgcaaatg ccgtaatgta
    16021 caaaggtgtt aaatccttcg catgcctccc attcgtgctt gggggactca aggctttatg
    16081 tgagtcagag cccagtgtta aggcacaact gcaagtggag gaacgagccc agcttttgaa
    16141 gtcactccaa cacatgaaaa tcctggaatg cgggggggca atgctcgaag tgagtgtggc
    16201 aagctgggct atagagaata ggattcctat atctattggg attggtatga cagagacagg
    16261 cggcgctctt ttcgctgggc cggtccaagc aattcaaacc ggctttagca gtgaagataa
    16321 atttatcgag gacgcaacct acttgctggt caaggacgat tacgagagcc acgcagagga
    16381 ggacataaac gagggcgagt tagtggtaaa gtccagaatg ttacctaggg gttatttggg
    16441 ctataacgac ccctctttct ctgttgacga tgctggttgg gttactttca aaactggaga
    16501 tcgatattca gtcactcctg acggcaaatt ctcctggctt ggtcgaaata ctgatttcat
    16561 tcagatgacg agtggtgaga ctctcgatcc ccgtccgatt gagtccctcc tttgtgagtc
    16621 cagccttata tcacgagcct gtgtcatcgg ggataagttc ctgaacggac cagccacagc
    16681 tgtatgcgct attatagaac tcgaaccaac gactgttgag aaaggccagg ctcacagcag
    16741 ggatattgcc cgaatctttg cccccatcaa tcgagatctt cctcccccgt tacgtatagc
    16801 atggagtcac gtacttgtac ttcagccaag cgagaaaata ccgatgacga aaaagggtac
    16861 aatcttccgt aagaaaatcg aacaggtttt cggttccgct ttaggaggca gtagcggcga
    16921 caactcacaa gcaaccactg acgcttcagt agttaggagg gatgagttga gcaacacggt
    16981 caaacatatc ataagcagag ttttgggagt ctccgacgat gaactccttt ggacactgag
    17041 ctttgctgag cttggcatga cgtccgctct tgctactaga atagcaaatg aattgaacga
    17101 agttctggta ggtgtgaacc tccccatcaa tgcatgctac attcatgtgg acctgccttc
    17161 tttgtcaaat gccgtgtatg caaagttagc acaccttaaa ctcccggata ggacgcccga
    17221 gccaaggaag gccccagttg aaaatcccgg tggaaaggaa atcgtaatag taggtcaggc
    17281 ttttaggctt ccggggagta taaatgatgt cgcttctttg agggacgctt ttttggctag
    17341 acaagcatcc agtattatta ctgaaatacc gccagataga tgggaccacg catcatttta
    17401 tccaaaagac atacgattta ataaagcagg gctcgtcgac atcgctaact acgaccacag
    17461 ttttttcgga ttgacagcca cggaggcact gtacctgtca ccaacgatgc gtttggcact
    17521 tgaagtgtcc tttgaagcct tggagaatgc taatataccc gtgtcccagt taaagggctc
    17581 tcaaacagct gtatatgtcg caaccaccga cgacgggttt gagacactgc tgaatgctga
    17641 ggcaggatat gatgcataca cacgattcta tggcactggt agggccgcct ctacggccag
    17701 tggacgaatt agttatttac tcgacgtaca cggcccttca attaccgtag acactgcttg
    17761 ttcaggcggt gctgtttgca tagaccaggc catagactac ctccaatcct ctagcgcagc
    17821 tgataccgcc ataatctgcg ccagcaacac tcactgctgg ccaggttcct tcatgtttct
    17881 gagtgctcaa ggtatggtaa gttccggggg gcgatgtgca acttttacga cggatgccga
    17941 cggctatgta ccctctgaag gtgctgtggc atttatcctc aaaacccgag aagcagctat
    18001 gagagataaa gatactatct tggcaactat caaagcaacg cagattagtc ataatggtcg
    18061 ttcccaaggt ttagtcgcac caaacgtcaa ttcacaagct gatttacaca gaagcttatt
    18121 gcagaaagct ggtttgagtc cagccgacat tcacttcatc gaagctcacg ggacaggaac
    18181 gtcactcggt gaccttagtg aaatacaagc aattaatgat gcctacacgt catctcagcc
    18241 tcgaacagcc ggacccttaa tagtaagcgc aagtaaaacc gtcattggtc acacagagcc
    18301 tgctggaccg ttggtaggca tgctctcagt tctcaactcc tttaaagagg gcgcagttcc
    18361 cggcttagct cacctgacag cagataacct caatccggct ttagactgct cctccgtccc
    18421 tttacttatc ccctaccagc ctgtccactt ggcagctcct aagccacaca gggctgctgt
    18481 gatgagttat ggcttctccg gcactcttgg aggtatagtt cttgaagcac cggatgagga
    18541 aaggcttgag gaagaaccac ccaacgataa gcctatgtta tttgttgtta gcgccaaaac
    18601 tcatactgca ttgattgagt accttggacg ataccttgaa tttctgctcc aggccaatcc
    18661 ccaagacttc tgcgatatct gttacactag ctgcgttgga cgtgagcatt acaggtaccg
    18721 ttttgcatgt gtggcaaacg atatggaaga tttaattggc caacttcaga agaggcttgg
    18781 gtctaaggtt ccacctaaac cgtcctataa aagaggagcc ctcgcttttg ctttcagtgg
    18841 acaagggacc cagtttcgtg gaatggcaac agaattggct aaggcatata gtggttttcg
    18901 taagatagtc agtgatcttg ctaaaagagc atcagaactg tccggccatg caatcgacag
    18961 gtttctgctt gcatatgaca ttggggctga gaatgttgca ccggatagtg aagctgatca
    19021 aatctgcata ttcgtttacc aatgtagtgt tctcagatgg ctccaaacaa tggggattcg
    19081 tccgtcagcc gtgatcggtc acagcctcgg tgagattagc gcatccgtag ctgctggagc
    19141 attgtccttg gactctgccc ttgacctcgt aataagtaga gccaggttac tgaggtcatc
    19201 tacgaatgcc ccagctggca tggcagctat gtctgcatcc caggatgaag tagtcgaact
    19261 cataggaaag ctcgatttag ataaagctaa tagcctcagc gtgtccgtaa taaacggccc
    19321 ccagaacacc gttgtttcag gatcttctgc agcaatagaa agtatcgttg ctctcgcaaa
    19381 gggacgaaaa atcaaggcca gcgcactcaa cattaaccag gcattccata gtccttacgt
    19441 cgattctgca gtacccggct tacgagcttg gagtgagaaa catatatcat cagctcgacc
    19501 attgcaaatc cctttgtact ctaccctctt gggcgctcaa gtttctgagg gtcaaatgtt
    19561 aaatcccgat cactgggtag accatgctag gaaacccgtt cagttcgcac aagcagccac
    19621 tataatgaag gaatctttca ccggagtaat aatcgatatt ggacctcaag ttgtagcatg
    19681 gtccctgctc ttaagcaatg gtcttacttc cgtgaccgca ctcgccgcaa agcgtggccg
    19741 ttcacagcaa gtggccttcc tctccgcctt agctgatctt tatcaggact acggcgtcgt
    19801 accagacttt gttggactgt acgcacaaca ggaggacgct tccagactga aaaagaccga
    19861 tatcttaact tacccctttc agagggttcg acgttaccct tcctttatac cctctagaag
    19921 ggcacccacc cacgctcatg tccaagacga ggaaactctg tcatcaggat catcaactcc
    19981 aaccttagaa aatactgacc tggacagtgg gaaggaatct ctgatgggac caacaagagg
    20041 gcttttgcga gtcgatgatc ttagggactc aatagtcagt tcagtcaaag atgtcctgga
    20101 attgaagagt aacgaagatc tggacctcag cgagtccctt aatgctcttg gcatggactc
    20161 tataatgttc gctcaattac gaaaaaggat aggggagggc ctcggcctgt ccgttccaat
    20221 ggtttttctc agcgatgcct tcagtattgg agaaatggtc agtaacctcg tggaacaggc
    20281 cgaagcttcc gaggacaatt gagcttctct agctagagtc gatcgacaag ctcgagtttc
    20341 tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc
    20401 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
    20461 caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat cgctgttaga
    20521 gctctcccgg cgcgccctat ggagccagtt agtggattca tttgtgaaac agtttggaaa
    20581 atgttgcggt taagaactgt attgccaaaa ataaattaaa tgtttttcat cattactcat
    20641 tggtgactga aatgaattga ggtccctata ctcttattgc ttctcagcat cgagttgttt
    20701 gtgcatcacc attgtcatga ctcatgattt ttagaatcat ttatgcaatt catcatctta
    20761 atcattatca ttataggatg attgtatcat atataacagt cgaaagggaa gggccaaaaa
    20821 aagtgaaata aagttttgtt ctttgtaatg gaagaaaaag ttgggaggag aatcatggtt
    20881 tcactatggt tctttggaat tttgattttg gtagttagtg cccaggttcc gggaaccaaa
    20941 ttctgatcat actacaaaaa aatatcttgc ggattattgt agctcttgtc ttgcataaca
    21001 tgaagcttgg aattaactaa agaaagtttc ttagagattc ttcttcttgt ttgttctcta
    21061 aagaatctaa cactctttgt aatgtttatc taaacttgga actaattaga tgtgaaacac
    21121 tacaattcat taaacatgag atcatgcgta taggaatttg gttcctgctg caccgtggca
    21181 agtcgtacac catatacctt tttccctatt ttattcatat tttactttat attcacttta
    21241 caataaatat ctttagatat ttctacaata agatccttat ctctctccat ggtttaatta
    21301 aaaataaatc caagaaaagt gcataaaaag atatgtactt tttattcctc tacccaatca
    21361 cttcacagct tcactcacgc gcgtgttctt tgatatcaga tagatacgtc tacgtgagat
    21421 acacgctctt gttaatcaag tggtgatgaa acagaactta ctcgcctcgg tgtccgattc
    21481 tactcagaag atatttacta taaatatctg atttctctcg actttgacga aaatcaatgc
    21541 gtataaatat ctctctgtct agcttattcg agagattaag caaactcagc tctcgtagca
    21601 tagctattac atgtggtgtc gtcctcgcct ctgctattgc ttttcccatc atacgacgag
    21661 attatcagac gttcttagag gttggtccta gctatgcccc tcaaaatttt cgaggctaca
    21721 ttatcgtttg cgtactcagc ttgttccgac aagagcaaaa agggttggca atctacgaca
    21781 gactcccaga gaagagaaga tggctggccg atctcccatt tcgtgaaggt actagaccta
    21841 gtataacatc acacataata cagagacagc gaactcaact tgtagaccaa gagtttgcca
    21901 cacgagaact gatagacaaa gttattccca gggtgcaagc acgacataca gataaaacat
    21961 ttttgagcac gagcaagttt gaatttcatg ctaaagccat tttcctgttg ccaagtattc
    22021 ctataaacga ccccttaaac atacctagtc atgatacggt tcgtagaacg aaacgagaga
    22081 ttgcacacat gcatgactac cacgactgta ctcttcactt agctcttgca gctcaagatg
    22141 gaaaagaagt cctgaaaaaa ggctggggcc aacgtcatcc gcttgccggg ccgggtgtac
    22201 ctgggccgcc gacagaatgg actttcttat acgcaccccg taatgaggaa gaagctaggg
    22261 tagtagaaat gattgtcgag gcaagtatcg gatacatgac taatgatccg gcaggtaaaa
    22321 tcgtcgagaa tgccaaatga gcttgttcga gtattatggc attgggaaaa ctgtttttct
    22381 tgtaccattt gttgtgcttg taatttactg tgttttttat tcggttttcg ctatcgaact
    22441 gtgaaatgga aatggatgga gaagagttaa tgaatgatat ggtccttttg ttcattctca
    22501 aattaatatt atttgttttt tctcttattt gttgtgtgtt gaatttgaaa ttataagaga
    22561 tatgcaaaca ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa tgaccgaagt
    22621 taatatgagg agtaaaacac ttgtagttgt cgctgttaga gctcagtg
//