| 质粒名称: | pCytERM-mCherry |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000746 |
| 产品用途: | Expresses CytERM-mCherry in mammalian cells |
| 存储实验室: | Zhifei Fu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | mCherry |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A highly stable monomeric red fluorescent protein for advanced microscopy
Xiong H., Chang Q., Ding J., Wang S., Zhang W., Li Y., Wu Y., Lin P., Yang C., Liu M., Fang G., Yang Y., Xie J., Qi D., Jiang T., Fu W., Hu F., Chen Y., Yue R., Li Y., Cui Y., Li M., Fan S., Yang Y., Xu Y., Li D., Zhang F., Zhao H., Wu C., Zheng Q., Piatkevich KD., Fu Z.
Nat. Methods 2025 1–11 (2025)
|
pCytERM-mCherry
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCytERM-mCherry载体质粒基本信息
pCytERM-mCherry质粒图谱载体图谱和pCytERM-mCherry载体序列质粒序列多克隆位点信息
pCytERM-mCherry质粒载体简介
pCytERM-mCherry质粒序列载体序列
LOCUS pCytERM-mCherry 4803 bp DNA circular SYN 22-OCT-2024
DEFINITION TSN20241013-0571-00010_746_20241014-BAN4_E02_E02_i20.1.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4803)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4803
/mol_type="other DNA"
/organism="synthetic DNA construct"
polyA_signal 63..110
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 439..1027
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer 1202..1505
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1506..1709
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 1761..1847
/codon_start=1
/label=cytochrome p450
/translation="MDPVVVLGLCLSCLLLLSLWKQSYGGGKL"
regulatory 1893..1902
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1899..2609
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
polyA_signal 2732..2853
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2860..3315)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3342..3446
/gene="bla"
/label=AmpR promoter
promoter 3448..3805
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 3840..4634
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
1 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
61 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
121 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
181 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
241 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
301 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
361 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
421 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
481 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
541 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
601 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
661 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
721 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
781 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
841 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
901 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
961 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
1021 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
1081 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
1141 ttagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
1201 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
1261 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
1321 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
1381 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
1441 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
1501 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
1561 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
1621 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
1681 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct
1741 accggactca gatctccacc atggatcctg tggtggtgct ggggctctgt ctctcctgtt
1801 tgcttctcct ttcactctgg aaacagagct atgggggagg gaagcttcga attctgcagt
1861 cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg
1921 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
1981 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
2041 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
2101 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
2161 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
2221 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
2281 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
2341 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
2401 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
2461 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
2521 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
2581 ccggcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca
2641 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
2701 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
2761 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
2821 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
2881 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
2941 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
3001 tttggaacaa gagtccacta ttaaagaacg tggactccga cgtcaaaggg cgaaaaaccg
3061 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
3121 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
3181 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
3241 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
3301 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
3361 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
3421 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
3481 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
3541 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
3601 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
3661 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
3721 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
3781 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
3841 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
3901 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
3961 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
4021 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
4081 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
4141 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
4201 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
4261 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
4321 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
4381 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
4441 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
4501 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
4561 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
4621 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
4681 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
4741 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
4801 cca
//
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