| 质粒名称: | pU6-ERCC6-PegRNA-H1-Nicking sgRNA |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002212 |
| 产品用途: | Simultaneously express pegRNA and nicking sgRNA targeting the ERCC6 gene |
| 存储实验室: | Zhuobin Liang Lab 梁卓斌课题组 |
|
载体骨架基本信息 |
|
| 载体名称: | pU6 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | U6, H1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ERCC6-pegRNA-nicking-sgRNA |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single-and Multi-Locus Genome Engineering
Zhichao Qiu, Keke Sun, Qingwei Zeng, Ziwei Luo, Xinran Liu, Yaping Li, Xiang Lei, Ruilin Zhao, Zhen Zhang, Damia Romero-Moya, Yuan Yang, Xuan Wang, Ehsan Hashemi, Joe Z. Zhang, Xiaolong Wang, Alessandra Giorgetti, Zhuobin Liang
bioRxiv 2026.02.20.706938
|
pU6-ERCC6-PegRNA-H1-Nicking sgRNA
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pU6-ERCC6-PegRNA-H1-Nicking sgRNA载体质粒基本信息
pU6-ERCC6-PegRNA-H1-Nicking sgRNA质粒图谱载体图谱和pU6-ERCC6-PegRNA-H1-Nicking sgRNA载体序列质粒序列多克隆位点信息
pU6-ERCC6-PegRNA-H1-Nicking sgRNA质粒载体简介
pU6-ERCC6-PegRNA-H1-Nicking sgRNA质粒序列载体序列
LOCUS pU6-ERCC6-PegRNA-H1-Nicking sgRNA 5311 bp DNA circular SYN 07-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5311)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 21, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5311
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 72..320
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 72..92
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 243..262
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
primer_bind 318..344
/label=OL2777
misc_feature 322..341
/label=ERCC6 spacer
misc_RNA 342..417
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
misc_feature 418..432
/label=RTT
primer_bind complement(428..468)
/label=OL2778
misc_feature 432
/label=a to g
misc_feature 433..450
/label=PBS
primer_bind 461..477
/label=OL2772
misc_feature 463..673
/label=H1 promoter
primer_bind 593..612
/label=H1
/note="Human H1 promoter, forward primer"
primer_bind complement(665..706)
/label=OL2779
misc_feature 683..702
/label=ERCC6 nickingRNA
misc_RNA 703..778
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
misc_feature 830..947
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 996..1506
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 1425..1443
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 1528..2127
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(1528..1547)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 2024..2044
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
polyA_signal 2274..2395
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2285..2304)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2339..2358
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2643..2660)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2814..3402)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2894..2913)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3573..4433)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4196..4215
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4434..4538)
/gene="bla"
/label=AmpR promoter
rep_origin 4565..5020
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4652..4671)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4862..4883
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 5151..5199
/note="synthetic polyadenylation signal"
misc_feature 5213..5304
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 5253..5272
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 ggtaccgatt agtgaacgga tctcgacggt atcgatcacg agactagcct cgagcggccg
61 cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
121 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
181 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
241 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
301 tgtggaaagg acgaaacacc gtcaatgctt tcatcaccag agttttagag ctagaaatag
361 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcact
421 tctatgccat ccggtgatga aagcattgat tttttttgaa cgctgacgtc atcaacccgc
481 tccaaggaat cgcgggccca gtgtcactag gcgggaacac ccagcgcgcg tgcgccctgg
541 caggaagatg gctgtgaggg acaggggagt ggcgccctgc aatatttgca tgtcgctatg
601 tgttctggga aatcaccata aacgtgaaat gtctttggat ttgggaatct tataagttct
661 gtatgaggac cacgaaacac cggtcagtga ttagtggaaa tggttttaga gctagaaata
721 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
781 tttttaaaga attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa
841 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
901 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
961 gggacagcag agatccactt tggccgcggc tcgagggggt tggggttgcg ccttttccaa
1021 ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc
1081 ggcgccgacc ctgggactcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc
1141 gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt
1201 tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc
1261 ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc
1321 aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag
1381 gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc
1441 aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct
1501 ccccaggggg atccaccgga gcttaccatg accgagtaca agcccacggt gcgcctcgcc
1561 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
1621 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
1681 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
1741 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
1801 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
1861 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
1921 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
1981 gagcgcgccg gggtgcccgc cttcctggaa acctccgcgc cccgcaacct ccccttctac
2041 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
2101 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa
2161 aggagcgcac gaccccatgc atcggtacct ttaagaccaa tgacttacaa ggcagctgta
2221 gatcttagcc actttctaga gtcggggcgg ccggccgctt cgagcagaca tgataagata
2281 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
2341 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
2401 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
2461 caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg atgcccttga
2521 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
2581 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct
2641 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
2701 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
2761 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
2821 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
2881 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
2941 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
3001 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
3061 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
3121 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
3181 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
3241 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
3301 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
3361 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
3421 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
3481 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
3541 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
3601 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
3661 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgggaccca
3721 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
3781 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
3841 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
3901 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
3961 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
4021 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
4081 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
4141 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
4201 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
4261 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
4321 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
4381 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
4441 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
4501 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
4561 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
4621 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
4681 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
4741 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
4801 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
4861 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
4921 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
4981 tttaacgcga attttaacaa aatattaacg cttacaattt gccattcgcc attcaggctg
5041 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gcccaagcta
5101 ccatgataag taagtaatat taaggtacgg gaggtacttg gagcggccgc aataaaatat
5161 ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg
5221 ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa
5281 gtgcaggtgc cagaacattt ctctatcgat a
//
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