pKeratin-tdStayGold

LOCUS      pKeratin-tdStayGold              6736 bp DNA     circular SYN 18-JUL-2024
DEFINITION  Keratin labeling with near-infrared fluorescent protein 
            miRFP670nano.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Oliinyk OS, Shemetov AA, Pletnev S, Shcherbakova DM, Verkhusha VV
  TITLE     Smallest near-infrared fluorescent protein evolved from 
            cyanobacteriochrome as versatile tag for spectral multiplexing.
  JOURNAL   Nat Commun. 2019 Jan 17;10(1):279. doi: 10.1038/s41467-018-08050-8.
  PUBMED    30655515
REFERENCE   2  (bases 1 to 6736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-17"; volume: "10(1):279. doi"; pages: " 
            10.1038/s41467-018-08050-8"
FEATURES             Location/Qualifiers
     source          1..6736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        86..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             625..1914
                     /codon_start=1
                     /label=Keratin18
                     /translation="MSFTTRSTFSTNYRSLGSVQAPSYGARPVSSAASVYAGAGGSGSR
                     ISVSRSTSFRGGMGSGGLATGIAGGLAGMGGIQNEKETMQSLNDRLASYLDRVRSLETE
                     NRRLESKIREHLEKKGPQVRDWSHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADD
                     FRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVK
                     GLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTV
                     LTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNG
                     ILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSS
                     NSMQTIQKTTTRRIVDGKVVSETNDTKVLRH"
     primer_bind     1319..1340
                     /label=0312Keratin-F
     primer_bind     1937..1958
                     /label=0901-TD-F
     CDS             1939..3378
                     /codon_start=1
                     /label=td8ox2StayGold
                     /translation="MASTGEELFTGVVPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKG
                     KYVCTSGKLPMSWAALGTSFGYGMKYYTKYPSGLKNWFHEVMPEGFTYDRHIQYKGDGS
                     IHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMNVSLPNEVQHIPRDDGVECPVTL
                     LYPLLSDKSKCVEAFQNTIIKPLHNQPAPDVPYHWIRKQYTQSKDDTEERDHIIQSETL
                     EAHLYSRTKLEGHGTGSTGSGSSGTASSEDNNMASTGEELFTGVVPFKFQLKGTINGKS
                     FTVEGEGEGNSHEGSHKGKYVCTSGKLPMSWAALGTSFGYGMKYYTKYPSGLKNWFHEV
                     MPEGFTYDRHIQYKGDGSIHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMNVSLP
                     NEVQHIPRDDGVECPVTLLYPLLSDKSKCVEAFQNTIIKPLHNQPAPDVPYHWIRKQYT
                     QSKDDTEERDHIIQSETLEAHL"
     misc_feature    1939..2616
                     /label=oxStayGold
     misc_feature    1951..1977
                     /label=n1
     primer_bind     2613..2638
                     /label=TD-F
     misc_feature    2617..2637
                     /label=PT
     misc_feature    2638..2700
                     /label=Tdtomato
     primer_bind     complement(2687..2708)
                     /label=TD-R
     misc_feature    2701..3378
                     /label=oxStayGold
     CDS             3379..3408
                     /codon_start=1
                     /label=c4
                     /translation="PWHEPSASAV"
     primer_bind     complement(3389..3408)
                     /label=0901-TD-R
     polyA_signal    3549..3670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3586..3605)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3640..3659
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3640..3659)
                     /label=SV40-R-Universal
     rep_origin      complement(3677..4132)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3814..3835)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4026..4045
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4159..4263
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4265..4622
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(4288..4308)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4473..4608
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4535..4554
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4558..4569)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4657..5451
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4711..4730)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5321..5340
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5639..5658)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5683..5730
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6059..6647
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6548..6567
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggataaccgt attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact tttcaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
      601 tgaaccgtca gatccgctag caccatgagc ttcaccactc gctccacctt ctccaccaac
      661 taccggtccc tgggctctgt ccaggcgccc agctacggcg cccggccggt cagcagcgcg
      721 gccagcgtct atgcaggcgc tgggggctct ggttcccgga tctccgtgtc ccgctccacc
      781 agcttcaggg gcggcatggg gtccgggggc ctggccaccg ggatagccgg gggtctggca
      841 ggaatgggag gcatccagaa cgagaaggag accatgcaaa gcctgaacga ccgcctggcc
      901 tcttacctgg acagagtgag gagcctggag accgagaacc ggaggctgga gagcaaaatc
      961 cgggagcact tggagaagaa gggaccccag gtcagagact ggagccatta cttcaagatc
     1021 atcgaggacc tgagggctca gatcttcgca aatactgtgg acaatgcccg catcgttctg
     1081 cagattgaca atgcccgtct tgctgctgat gactttagag tcaagtatga gacagagctg
     1141 gccatgcgcc agtctgtgga gaacgacatc catgggctcc gcaaggtcat tgatgacacc
     1201 aatatcacac gactgcagct ggagacagag atcgaggctc tcaaggagga gctgctcttc
     1261 atgaagaaga accacgaaga ggaagtaaaa ggcctacaag cccagattgc cagctctggg
     1321 ttgaccgtgg aggtagatgc ccccaaatct caggacctcg ccaagatcat ggcagacatc
     1381 cgggcccaat atgacgagct ggctcggaag aaccgagagg agctagacaa gtactggtct
     1441 cagcagattg aggagagcac cacagtgctc accacacagt ctgctgaggt tggagctgct
     1501 gagacgacgc tcacagagct gagacgtaca gtccagtcct tggagatcga cctggactcc
     1561 atgagaaatc tgaaggccag cttggagaac agcctgaggg aggtggaggc ccgctacgcc
     1621 ctacagatgg agcagctcaa cgggatcctg ctgcaccttg agtcagagct ggcacagacc
     1681 cgggcagagg gacagcgcca ggcccaggag tatgaggccc tgctgaacat caaggtcaag
     1741 ctggaggctg agatcgccac ctaccgccgc ctgctggaag atggcgagga ctttaatctt
     1801 ggtgatgcct tggacagcag caactccatg caaaccatcc aaaagaccac cacccgccgg
     1861 atagtggatg gcaaagtggt gtctgagacc aatgacacca aagttctgag gcatcgaatt
     1921 ctgcagtcga cggtacccat ggctagcact ggggaagaac tgttcacagg agtcgtgccg
     1981 tttaagttcc agttgaaggg cacgatcaat ggaaagtctt tcacagtgga aggagagggt
     2041 gagggtaata gccatgaagg ttcacacaaa ggcaagtacg tctgtacctc tggaaagctt
     2101 ccgatgtcat gggccgctct gggtacctcc tttgggtatg gcatgaaata ctatacaaag
     2161 tacccctcag ggttgaagaa ctggttccat gaggtcatgc ccgaaggatt cacctatgat
     2221 cgccacatcc agtataaggg cgacggctct attcatgcca aacatcaaca cttcatgaag
     2281 aacggcacct accataacat cgtggagttt actggacaag actttaagga gaatagccca
     2341 gttctgaccg gtgatatgaa cgtaagcctg cctaacgaag tgcagcacat tcccagggac
     2401 gatggggttg agtgtcctgt taccctgctg taccctctgt tgagcgacaa atccaaatgc
     2461 gtagaggcgt ttcagaacac cattatcaaa ccactgcata accaaccagc tcccgatgta
     2521 ccgtaccatt ggatacggaa acagtatacc cagtcaaaag acgacactga ggaacgtgac
     2581 cacattatcc agtccgagac acttgaagca cacctctatt cacgaacaaa actggagggg
     2641 catggcactg gctctactgg atccggctcc agtggaacag catcaagcga ggacaacaat
     2701 atggcctcca caggagagga gctgttcacc ggagtcgttc cattcaaatt tcagctgaaa
     2761 ggcaccatta atgggaaaag cttcacagtg gagggagaag gtgaaggtaa tagtcacgaa
     2821 gggagccaca agggaaaata cgtctgcact agcgggaaac tcccaatgtc ttgggctgca
     2881 ctggggacta gctttggcta cgggatgaag tactacacga agtatccttc cggcctcaag
     2941 aactggtttc acgaggtgat gccagaaggg tttacctatg atagacacat acagtacaag
     3001 ggcgacggca gtatccacgc gaaacaccag catttcatga agaatgggac gtaccacaac
     3061 attgtggaat ttacgggcca agacttcaag gagaattctc ccgtgcttac cggcgatatg
     3121 aacgtgagtc tgccaaatga ggtgcagcat atacctaggg acgatggtgt ggaatgtccc
     3181 gtcacactgc tgtatcctct gctcagtgat aaatctaagt gcgttgaggc cttccaaaac
     3241 accatcatca agcccttgca taatcagcct gcccctgatg tgccctatca ctggatccgg
     3301 aagcagtaca cacagtccaa ggatgacact gaggagagag atcacattat ccagagcgag
     3361 actctcgaag cccaccttcc atggcacgag ccttctgctt ctgccgtgta agatatccag
     3421 cacagtggcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac
     3481 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     3541 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3601 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3661 atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     3721 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     3781 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     3841 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     3901 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     3961 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     4021 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     4081 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
     4141 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4201 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4261 agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     4321 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     4381 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4441 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4501 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4561 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4621 aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     4681 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     4741 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     4801 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     4861 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     4921 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     4981 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     5041 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     5101 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     5161 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     5221 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     5281 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     5341 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     5401 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     5461 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     5521 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     5581 tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg
     5641 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     5701 ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     5761 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     5821 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     5881 caggccctgc catagcctca ggttactcat atatacttta gattgattta aaacttcatt
     5941 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6001 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6061 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6121 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6181 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6241 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6301 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6361 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6421 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6481 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6541 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6601 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6661 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6721 tatcccctga ttctgt
//