| 质粒名称: | pSF11-wmiRFP2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000241 |
| 产品用途: | Expresses miRFP2 in C. elegans neurons |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pSF11 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | tag168 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | wmiRFP2 |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rapid directed molecular evolution of fluorescent proteins in mammalian cells
Babakhanova S., Jung E.E., Namikawa K., Zhang H., Wang Y., Subach O.M., Korzhenevskiy D.A., Rakitina T.V., Xiao X., Wang W., Shi J., Drobizhev M., Park D., Eisenhard L., Tang H., Köster R.W., Subach F.V., Boyden E.S., Piatkevich K.D.
Protein Sci. 31, 728–751 (2022)
|
pSF11-wmiRFP2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSF11-wmiRFP2载体质粒基本信息
pSF11-wmiRFP2质粒图谱载体图谱和pSF11-wmiRFP2载体序列质粒序列多克隆位点信息
pSF11-wmiRFP2质粒载体简介
pSF11-wmiRFP2质粒序列载体序列
LOCUS pSF11-wmiRFP2 7035 bp DNA circular SYN 01-JUN-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7035)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7035
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 54..2717
/label=tag-168 promoter
primer_bind 2796..2816
/label=pSF11-Fw-Seq
misc_feature 2834..3775
/label=wmIRFP2
primer_bind complement(3820..3841)
/label=pSF11-Rv-Seq
promoter 4914..5018
/gene="bla"
/label=AmpR promoter
CDS 5019..5879
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
CDS 5217..5876
/label=AmpR
rep_origin 5974..6656
/label=ColE1 origin
rep_origin 6050..6638
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 6926..6947
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6962..6992
/label=lac promoter
/note="promoter for the E. coli lac operon"
misc_binding 6996..7018
/label=LacO
protein_bind 7000..7016
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind join(7024..7035,1..5)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gccggatcca
61 taggtatgcc tgtcattctc tatttttaaa cgttcctgca gcagtagcac aacagagcca
121 gcagttttcc tcttcttctc catcttcttc tgcttcttcc tctcttccca aaaaaaaaaa
181 aaaaaaaaaa gtgagggggc tcactgcccc cccccccctc ccccccccct cttctccttg
241 aagctcatcc agacgtcgct gtcctcctcc tcctcgtccc cggggcgctg tgtgtgtgtg
301 tgtgtgctcc gtgagcatgt agcagcttca gcccatttaa aggcgcacac tctctgcatc
361 tctgcgtgtc tcgacactcg ttttttctct tcttttctgc tgcacacaca cacacacaca
421 cacacacgct catgccagag cacttcttgc tccggggggc tccgcgcgga gcccgagagg
481 acctcctcct cctttttctt cttcgctttt tagttcttct tgcctcggtc tcgccccttg
541 ttttgctctc catgtagtat atacatctgg gtgtgtgtgt gtgtgtgcac ctttggagca
601 ttttttgaac ttttccagaa ctttggagca acattttcag cttgttcagt gcacttttta
661 ggctgaaaat cctattttta ggttttcgaa agtttttttg gagcattttt cgtgcaattt
721 aggtgcaaaa ttaaaaaaaa gggttttgta gtgaattaaa atttttttga aactttcgcc
781 tttttaaaaa aatgtttcta atatctttaa aaattggaat attttggaaa acaatttgtt
841 ttcaattggc aatttattaa ttctgagctt ctgagcgtaa attttttttt attttggagc
901 aattctgaga tgtcttagtg catttttcgg gcatacataa aaaaaaattt atcttaataa
961 taaaaacctt aaatttaggc ttcaccgtct ggaaattcaa ttttttcagc tgaaaaactt
1021 tcaaaaaaaa aaaaattaaa ttttcacatt tattttctct ggaattttgg agcaatttca
1081 aagctgtctt agtgcatttt tagtacacaa atattttttc atttttctta aattttaata
1141 atctaagctt aaataaaggc ttcaccgtct gaaaatgcaa cttttttcag atgaaatatt
1201 aaaaaaaaaa acctctttta ttaattttgt gctccaaact ttcccacggt acgcaaatca
1261 tgtaatatgt acttttaaac attatttttc caatcaaaaa tatccaacag cttttcctta
1321 caatttgaaa atttttatta aaatttcaaa aaaatttttt ttttttttta atttcgaatt
1381 tttttaaact tgttgtaact tttttccctg tcggcgccaa ttaacaaaat acgtagaatt
1441 ttgcgcttat tgttttgtta gttaacaaat ttttgacaac tttcttatca gctgagattt
1501 aggtgtttca gttcgcaatc tgcgtctctc cttgctctcc tctattttaa aacccccata
1561 actttgttct tgtgagagtt atcaaaaaat tgtcaactaa caaaatatag agcacaaaaa
1621 tatgcacatt ttgtatgttg acaatttttt gatacctttt ttagttttcg agatatctta
1681 gttttagttt agctgtacct ttttctggca aaaaacttcc aaatttcggc aatttttcgg
1741 caaatttccc ggcaaatttc gccaagtaca tcgggctctc agacaagtgg tctgtgagtg
1801 gaagtcgata aaagtagttt acgtgctctc cgtccccccg cttcttctcc gtctctgctt
1861 ctatttctcc attgactagt cccactactt tttacggggc ggagggctct cttgcactcc
1921 agcttcttct ctttttgtgc tcttttttgc ccgtatgtgg gtgctcattt atttttcttt
1981 ggttttaaaa taaattcggt ttattaggct actcgtaaaa atgaagaaaa aacttcaaat
2041 tcaaacgaca ttaaaaaaaa atttaataaa aattttttga attgctcaaa agccccacct
2101 atatatatat atagctccgc ctacttctcg cacagacccc gcccactttt tgactccgcc
2161 cccttattgg caaaagttct tgaaaaaaat aaaaataaaa attcaaattg aaacaatttt
2221 tttttaatga agtagttttt ttctaaattg atgaaaattt ctaaacagtt tacaagcacc
2281 gcccactttt tgactccgcc cctttgttgg aaaaattctt gtaaaaaaaa ataaaaaata
2341 aaagcttctc tttcaaacga tttttagttg gtttttgtat gaaaagtttt tttcctaaaa
2401 tagaaatgtc tgaataattt gcaagctccg cccttttctt acccagactc cgccccttta
2461 ttgactaatt tttttttgtc catttacctg aagttcaaaa aaaaaattta cagtttcata
2521 gagtaataat ttttcaaaaa tctctttttc caatgtttaa tgtgtcatta taaacccgcc
2581 cagttttaag ctccgcccat ttaggccccg cccatttatg acccaaatat ttttttctct
2641 attcacccgt ggatttccct ccaacaagtt accccaaaaa taaaaataaa aattcctgca
2701 ggcgcccacc gggatccggc gcgcctctag aggatccccg ggattggcca aaggacccaa
2761 aggtatgttt cgaatgatac taacataaca tagaacattt tcaggaggac ccttggctag
2821 cgtcgacggt accatggtag ccggacacgc ctcaggtagt ccagacttcg gcaccgcctc
2881 gccaagtgac tgcgagcatg aggagattca tcttgctggc tctatccaac cacacggcac
2941 tctgttggtc gtgtcggaac ctgaccatag aatcattcaa gcatccgcga acgcggctga
3001 atttttaaac ctgggttccg tattgggtat accattagcg gaaatagacg gcgacctcct
3061 cataaaaatc ttgcctcacc ttgacctcac cgcagaaggc atgccggtcg cagttcgttg
3121 ccgaatcgga aatccttcga cagagtatga tggccttatg caccgacctc cggagggcgg
3181 tctgattata gagctggaga gagcaggccc atctatagac ctttccggaa cacttgcacc
3241 ggcgctcgaa cgaataagaa cggcaggcag tttgcgtgca ttgtgcgatg acacagtctt
3301 actctttcag caatgcacag gttatgaccg agtcatggtg tatcgtttcg atgaaagagg
3361 tcatggcgag gtttactcgg agatccatgt gaccggactc gaatcatatt ttggcaatcg
3421 ttacccgagt tcgactgtac cgcaaatggc aagaagatta tatgtacgtc aacgtgtgcg
3481 agtgttagtt gatgtaactt accagccagt cccgttggag ccgcgactct caccattaac
3541 gggcagagat ctggatatgt ctggatgctt cttacgttcg atgtcaccat gtcatctgca
3601 gttcctgaaa aacatgggcg ttagagccac tctggttgta tcgctcgttg taggaggcaa
3661 actttgggga ctggtaatat gccatcatta ccttcctcga ttcatacatt tcgaattacg
3721 agctatttgc gttctgctgg ctgaagcaat agctacacga atcaccgcct tgggttaaga
3781 gctccgcatc ggccgctgtc atcagatcgc catctcgcgc ccgtgcctct gacttctaag
3841 tccaattact cttcaacatc cctacatgct ctttctccct gtgctcccac cccctatttt
3901 tgttattatc aaaaaaactt cttcttaatt tctttgtttt ttagcttctt ttaagtcacc
3961 tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa
4021 aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca caatgttctg
4081 tgtacacttc ttatgttttt tttacttctg ataaattttt tttgaaacat catagaaaaa
4141 accgcacaca aaatacctta tcatatgtta cgtttcagtt tatgaccgca atttttattt
4201 cttcgcacgt ctgggcctct catgacgtca aatcatgctc atcgtgaaaa agttttggag
4261 tatttttgga atttttcaat caagtgaaag tttatgaaat taattttcct gcttttgctt
4321 tttgggggtt tcccctattg tttgtcaaga gtttcgagga cggcgttttt cttgctaaaa
4381 tcacaagtat tgatgagcac gatgcaagaa agatcggaag aaggtttggg tttgaggctc
4441 agtggaaggt gagtagaagt tgataatttg aaagtggagt agtgtctatg gggtttttgc
4501 cttaaatgac agaatacatt cccaatatac caaacataac tgtttcctac tagtcggccg
4561 tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
4621 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
4681 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
4741 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
4801 taccgcatca ggcggcctta attaagggcc tcgtgatacg cctattttta taggttaatg
4861 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
4921 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
4981 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
5041 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
5101 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
5161 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
5221 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
5281 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
5341 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
5401 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
5461 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
5521 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
5581 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
5641 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
5701 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
5761 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
5821 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
5881 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
5941 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
6001 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
6061 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
6121 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
6181 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
6241 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
6301 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
6361 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
6421 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg
6481 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
6541 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
6601 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
6661 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
6721 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
6781 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
6841 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
6901 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
6961 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
7021 acacaggaaa cagct
//
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pSF11-wmiRFP2质粒载体应用举例
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