| 质粒名称: |
pAAVLINK-3'-MED12L-FLAG
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001708 |
| 产品用途: | 3' AAVLINK plasmid for MED12L expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | MED12L |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-3'-MED12L-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-3'-MED12L-FLAG载体质粒基本信息
pAAVLINK-3'-MED12L-FLAG质粒图谱载体图谱和pAAVLINK-3'-MED12L-FLAG载体序列质粒序列多克隆位点信息
pAAVLINK-3'-MED12L-FLAG质粒载体简介
pAAVLINK-3'-MED12L-FLAG质粒序列载体序列
LOCUS pAAVLINK-3'-MED12L-FLAG 7247 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7247)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 3, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7247
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
protein_bind 145..178
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..3986
/codon_start=1
/label=MED12L
/translation="GCVVWSSMS*TPQNVLPLKDAF*PTSMISMCHVATSEVNLETSSV
VPVQK*SKPYIIT*CLQIRTCDGIQTS*WILLRIPQPAASTTQCWARSSVTMRPIATAL
SAIHS*MYVWAIRMLAGLTT*PISPLSLRLAALFLVQNGWGF*RLFVVLQITCGGLMMY
FAL*M*VTFHSMIH*LLSLLF**HDSVFPWRTSCSMSHFPLF*QQLVGMRTPSLGRE*H
ADSCFISSELPRPASYLKQRANLSLE*DHLVIDTS*PLLTTALKWEPCLLS*KQL*CLE
MPKLAITVSAL*RMMTSP*EVCDVMGMLMISGLPHKIQNPVGKAFP*KLPI*ENTLDMY
*GLSVNRNG*ESIA*KNLKDYVQTKNLYWTLCFQICKHRNYCSLSVILMALKNVPRGTI
CKDSTLSVFFRILSSGH*GNPG*NSS**SNSA*RTLALVLWPK*TTYWTILQRQQ*RYS
SSLQT*IILLILA*ASSTQTVLEVLIQVARDRME*RHS*VPPNAGVYGWWPPSSPGCQL
LCKEEC*KPLGKSWRRDSTWVLLPKRKGTDRNRKVCLF*VNNPSSHWYLPALRDKMNKG
KAS*HLSRIKLTRF*VTGEKNDTKMT*KRGR*CTKHCNSA*IW*EECLTRCRGAPSGLQ
TGPCYSFRSLLQELLTCTLTMNYSQQFLTCWVF*SMER*PLTYQMHPLGDLKRTSVHT*
I**RN*KKS*ETSDQKVLTKFDSYYLCRNRHVMSSLVNLWVP*LTQKETKLLDLTL*IK
NRVSRSLRSRRCPRGTCLRVRRTQLLCPGPGLGQSEWTEE*SSTRSSITSCCITHTPCP
SPAVTTSSHCPCLLRRKRKSPHLQFLRNQKGSPLSCQIREKPQQMKKRKQKEGSARRNL
AQELMNIHRATYTECLLITRLSPPK*CTIHSPPCGVTTSWASPSSPAFSFRTNLLLQVA
PDWTLQAPLSQPTPNKLCQTCYSGAQAP*CSRLLFMQSHRSSS*YR*SFCSSSSNSDFS
GKPRLGLSNRASRGTRLLSLLRKHGPPLSSLSIQGCSKHRPCHRAIQCMGHRCLCSRHR
SSRLAVWSCLPAITPEPIRPHIPTPC*WKDSDRFSSSRVAMFSSRPRRTCSP*LALRD*
TIRLYSRALWWAGELMLC*LLHIQTFPPCPCLRIP*DPDSRKFDSSRDSSRCSSPSSPS
PSSLPSPSSPRSPRVRPLVSKQCSPSSPCFPGKACSRPSSSSRRPPWCGSSRSSFPATS
HSKE*LRMGILHT"
misc_feature 2003..2008
/label=SDS
misc_feature 2129..2134
/label=SDS
misc_feature 2675..2680
/label=SDS
misc_feature 2742..2747
/label=SDS
misc_feature 2749..2754
/label=SDS
misc_feature 2928..2933
/label=SDS
CDS 3993..4058
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4062..4493
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4206..4211
/label=SDS
repeat_region 4510..4650
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4510..4639
/label=AAV2 ITR
rep_origin 4725..5180
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5462..5566
/gene="bla"
/label=AmpR promoter
CDS 5567..6427
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6598..7186
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag ggttgtgtgg tgtggtcaag catgtcgtaa
301 acccctcaga atgttcttcc cctgaaagat gcattttagc ctacctctat gatctctatg
361 tgtcatgtag ccacctcaga agtaaatttg gagacctctt cagtagtgcc tgttcaaaag
421 taaagcaaac catatataat aacgtgatgc ctgcaaattc gaacttgcga tgggatccag
481 acttcatgat ggattttatt gagaatccct cagcccgcag catcaactac tcaatgctgg
541 gcaagatcct cagtgacaat gcggccaatc gctacagctt tgtctgcaat acactcatga
601 atgtatgtat gggccatcag gatgctggca ggattaacga catagccaat ttctcctctg
661 agcttacggc ttgctgcact gttcttagtt cagaatggct gggggttctg aaggctcttt
721 gttgttcttc aaatcacgtg tgggggttta atgatgtact ttgcactgta gatgtgagtg
781 acctttcatt ccatgattca ttagctactt tcattgctat tctgatagca cgacagtgtt
841 tttccctgga ggacgtcgtg cagcatgtcg cacttccctc tcttctagca gcagcttgtg
901 gggatgcgga cgccgagcct ggggcgagaa tgacatgccg actcttgctt catctcttcc
961 gagctcccca ggcctgcttc ttacctcaag caacgggcaa acctttccct ggaataagat
1021 catcttgtga tagacacctc ttagccgctg ctcacaacag cattgaagtg ggagccgtgt
1081 ttgctgtctt aaaagcaatt atgatgcttg gagatgccaa aattggcaat aacagtgtca
1141 gctctttaaa gaatgatgac ttcaccatga gaggtttgcg atgtgatggg aatgctgatg
1201 atatctggac tgcctcacaa aatccaaaat cctgtgggaa aagcatttcc atagaaactg
1261 ccaatttaag agaatacgct agatatgtac tgaggactat ctgtcaacag gaatgggtag
1321 gagagcattg cttaaaagaa cctgaaagat tatgtacaga caaagaactt atattggacc
1381 ctgtgctttc aaatatgcaa gcacagaaat tactgcagct tatctgttat cctcatggca
1441 ttaaagaatg taccgagggg gacaatctgc aaagacagca cattaagcgt attcttcaga
1501 atcttgagca gtggacactg aggcaatcct ggttagaact ccagctaatg atcaaacagt
1561 gcttgaagga ccctggctct ggttctgtgg ccgaaatgaa caacttactg gacaatattg
1621 caaaggcaac aatagaggta ttccagcagt ctgcagacct aaataattct tctaattctg
1681 gcatgagcct cttcaaccca aacagtattg gaagtgctga tacaagtagc acgagacaga
1741 atggaataaa gacattccta agttcctccg aacgcagggg tgtatggttg gtggcccccc
1801 tcatcgccag gttgccaact tctgtgcaag gaagagtgct gaaagccgct ggggaagagc
1861 tggagaaggg acagcacttg ggttcttctt ccaaaaagga aagggacaga cagaaacaga
1921 aaagtatgtc tcttttgagt caacaaccct tcctctcact ggtacttacc tgccttaagg
1981 gacaagatga acaaagggaa ggcctcctaa catctctcca gaatcaagtt aaccagattt
2041 taagtaactg gagagaagaa cgataccaag atgacataaa agcgcggcag atgatgcacg
2101 aagcattgca actccgccta aatttggtag gaggaatgtt tgacacggtg cagaggagca
2161 cccagtggac tacagactgg gccctgctac tccttcagat cattacttca ggaactgttg
2221 acatgcacac taacaatgaa ttattcacaa cagttcttga catgctgggt gttttaatca
2281 atggaacgtt agcctctgac ctatcaaatg catcccctgg gggatctgaa gagaacaagc
2341 gtgcatacat gaatttagta aagaaactga aaaaagagct aggagacaag cgatcagaaa
2401 gtattgacaa agttcgacag ttactacctt tgccgaaaca gacatgtgat gtcatcactt
2461 gtgaacctat gggttccttg attgacacaa aaggaaacaa aattgctgga tttgactcta
2521 tagataaaaa acagggtctc caggtctcta cgaagcagaa ggtgtccccg tgggacttgt
2581 ttgagggtca gaagaaccca gctcctttgt cctgggcctg gtttgggaca gtccgagtgg
2641 accgaagagt gatcaagtac gaggagcagc atcacctcct gctgtatcac acacacccca
2701 tgcccaagcc ccgcagttac tacctccagc cactgcccct gcctcctgag gaggaagagg
2761 aagagcccac atctccagtt tctcaggaac cagaaaggaa gtccgctgag ctgtcagatc
2821 agggaaaaac cacaacagat gaagaaaaga aaacaaaagg aaggaagcgc aagacgaaat
2881 ctagctcaag agttgatgaa tatccacaga gcaacatata ccgagtgcct cctaattact
2941 cgcctatctc ctcccaaatg atgcaccatc cacagtccac cttgtggggt tacaacctcg
3001 tgggccagcc ccagcagccc ggctttttcc ttcagaacca atctcttact ccaggtggct
3061 ccagattgga ccctgcaggc tcctttgtcc caaccaacac caaacaagct ctgtcaaaca
3121 tgctacagcg gcgctcaggc gccatgatgc agccgccttc tcttcatgca atcacatcgc
3181 agcagcagtt gatacagatg aagcttctgc agcagcagca gcaacagcga cttctcaggc
3241 aagcccagac tcggcctttc caacagggcc agccggggga ccaggctgct ctctttgctg
3301 cgcaagcacg gccctcccct cagctccctc agtatccagg gctgcagcaa gcacagacca
3361 tgccacaggg ctatacaatg tatgggacac agatgccttt gcagcagaca tcgcagcagc
3421 aggctggcag tgtggtcctg tctcccagct ataactccag agcctatccg gccgcacatt
3481 ccaaccccgt gctaatggaa agactcagac agattcagca gcagccgagt ggctatgttc
3541 agcagcaggc ctcgccgtac ctgcagcccc tgactggctc tcagagactg aaccatcagg
3601 ctctacagca gagccctctg gtgggcgggg gaattgatgc tgtgctgact tctgcacatc
3661 caaaccttcc ctccgtgccc ctgcctcagg atcccatgag acccagacag ccgcaagttc
3721 gacagcagca gagactcctc cagatgcagc agccccagca gccccagccc cagcagcctc
3781 cccagcccca gcagtcctcg cagtcccaga gtcagaccct tggtctccaa gcaatgcagc
3841 cccagcagcc cttgtttccc aggcaaggct tgcagcagac ccagcagcag cagcagacgg
3901 ccgccttggt gcggcagctc cagaagcagc tttccagcaa ccagccacag caaggagtga
3961 ctccgtatgg gcatccttca cacttctgta cagactacaa agaccatgac ggtgattata
4021 aagatcatga catcgactac aaggatgacg atgacaagta actcgagata atcaacctct
4081 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
4141 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
4201 tttctcctcc ttgtataaat cctggttagt tcttgccacg gcggaactca tcgccgcctg
4261 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgtttat
4321 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatc tagctttatt tgtgaaattt
4381 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
4441 attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt taacggaccg
4501 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
4561 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
4621 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
4681 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
4741 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
4801 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
4861 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
4921 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
4981 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
5041 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
5101 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
5161 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
5221 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
5281 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
5341 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
5401 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
5461 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
5521 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
5581 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
5641 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
5701 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
5761 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
5821 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
5881 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
5941 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
6001 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
6061 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
6121 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
6181 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
6241 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
6301 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
6361 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
6421 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
6481 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
6541 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
6601 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
6661 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
6721 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
6781 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
6841 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
6901 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
6961 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
7021 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
7081 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
7141 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
7201 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
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pAAVLINK-3'-MED12L-FLAG质粒载体应用举例
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