| 质粒名称: | pCytERM-mRuby3 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000750 |
| 产品用途: | Expresses CytERM-mRuby3 in mammalian cells |
| 存储实验室: | Zhifei Fu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
CMV
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | mRuby3 |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A highly stable monomeric red fluorescent protein for advanced microscopy
Xiong H., Chang Q., Ding J., Wang S., Zhang W., Li Y., Wu Y., Lin P., Yang C., Liu M., Fang G., Yang Y., Xie J., Qi D., Jiang T., Fu W., Hu F., Chen Y., Yue R., Li Y., Cui Y., Li M., Fan S., Yang Y., Xu Y., Li D., Zhang F., Zhao H., Wu C., Zheng Q., Piatkevich KD., Fu Z.
Nat. Methods 2025 1–11 (2025)
|
pCytERM-mRuby3
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCytERM-mRuby3载体质粒基本信息
pCytERM-mRuby3质粒图谱载体图谱和pCytERM-mRuby3载体序列质粒序列多克隆位点信息
pCytERM-mRuby3质粒载体简介
pCytERM-mRuby3质粒序列载体序列
LOCUS pCytERM-mRuby3 4806 bp DNA circular SYN 22-OCT-2024
DEFINITION TSN20241013-0571-00010_750_20241014-BAN4_A03_A03_i20.1.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4806)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4806
/mol_type="other DNA"
/organism="synthetic DNA construct"
polyA_signal 97..218
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(225..680)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 707..811
/gene="bla"
/label=AmpR promoter
promoter 813..1170
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 1021..1156
/label=SV40 ori
/note="SV40 origin of replication"
CDS 1205..1999
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 2231..2278
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 2607..3195
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer 3370..3673
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3674..3877
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 3929..4015
/codon_start=1
/label=cytochrome p450
/translation="MDPVVVLGLCLSCLLLLSLWKQSYGGGKL"
regulatory 4061..4070
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 4067..4780
/codon_start=1
/label=mRuby3
/translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
AKYSNLGGGMDELYK"
ORIGIN
1 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
61 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
121 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
181 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa
241 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
301 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
361 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
421 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
481 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
541 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
601 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
661 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
721 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
781 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
841 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
901 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
961 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
1021 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
1081 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
1141 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
1201 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
1261 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
1321 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
1381 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
1441 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
1501 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
1561 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
1621 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
1681 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
1741 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
1801 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
1861 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
1921 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
1981 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
2041 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
2101 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
2161 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
2221 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
2281 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
2341 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
2401 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
2461 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
2521 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
2581 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
2641 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
2701 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
2761 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
2821 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
2881 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
2941 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
3001 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
3061 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
3121 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
3181 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
3241 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
3301 gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
3361 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
3421 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
3481 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
3541 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
3601 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
3661 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
3721 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
3781 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
3841 taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg
3901 ctagcgctac cggactcaga tctccaccat ggatcctgtg gtggtgctgg ggctctgtct
3961 ctcctgtttg cttctccttt cactctggaa acagagctat gggggaggga agcttcgaat
4021 tctgcagtcg acggtaccgc gggcccggga tccaccggtc gccaccatgg tgtctaaggg
4081 cgaagagctg atcaaggaaa atatgcgtat gaaggtggtc atggaaggtt cggtcaacgg
4141 ccaccaattc aaatgcacag gtgaaggaga aggcaggccg tacgagggag tgcaaaccat
4201 gaggatcaaa gtcatcgagg gaggacccct gccatttgcc tttgacattc ttgccacgtc
4261 gttcatgtat ggcagccgta cttttatcaa gtacccggca gacattcctg atttctttaa
4321 acagtccttt cctgagggtt ttacttggga aagagttacg agatacgaag atggtggagt
4381 cgtcaccgtc acgcaggaca ccagccttga ggatggcgaa ctcgtttaca acgtcaaagt
4441 cagaggggta aactttccct ccaatggtcc cgtgatgcag aagaagacca agggttggga
4501 gcctaataca gagatgatgt atccagcaga tggtggtctg aggggataca ctgatattgc
4561 actgaaagtt gatggtggtg gccatctgca ttgcaatttc gtaacaactt acaggtcaaa
4621 aaagaccgtc gggaacatca agatgcccgg tgtccatgcc gttgatcacc gcctggaaag
4681 gatagaggaa agtgacaatg aaacgtacgt agtacaacgc gaagtcgcag ttgccaagta
4741 ctccaatctt ggtggtggta tggatgaact ttataagtaa agcggccgcg actctagatc
4801 ataatc
//
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