| 质粒名称: | pN1-hCRAFi-CCR2null |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001174 |
| 产品用途: | null control |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1-EGFP |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 or DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | hCRAFi-CCR2null |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Genetically encoded biosensor for monitoring spatiotemporal dynamics of CCR2 ligands in culture and in vivo.
Xiao X., Wang C., Guo X., Xi F., Qian Q., Liang G., Chen M., Sun X., Szabo B., Jing M., Piatkevich K.D.
Nat. Methods 2025 1–11 (2025)
|
pN1-hCRAFi-CCR2null
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pN1-hCRAFi-CCR2null载体质粒基本信息
pN1-hCRAFi-CCR2null质粒图谱载体图谱和pN1-hCRAFi-CCR2null载体序列质粒序列多克隆位点信息
pN1-hCRAFi-CCR2null质粒载体简介
pN1-hCRAFi-CCR2null质粒序列载体序列
LOCUS pN1-hCRAFi-CCR2null 6062 bp DNA circular SYN 25-MAR-2025
DEFINITION PM20.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6062)
AUTHORS Kiryl Piatkevich
TITLE Direct Submission
JOURNAL Exported Aug 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6062
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 591..666
/label=MCS
/note="multiple cloning site of fluorescent protein
plasmids"
misc_feature 673..678
/label=Kozak
CDS 679..2502
/codon_start=1
/product="Isoform B"
/label=hCCR2B
/translation="MLSTSRSRFIRNTNESGEEVTTFFDYDYGAPCHKFDVKQIGAQLL
PPLASLVFIFGFVGNMLVVLILINCKKLKCLTDIYLLNLAISDLLFLITLPLWAHSAAN
EWVFGNAMCKLFTGLAHIGYFGGIFFIILLTIDRYLAIVHAVFALKARTVTFGVVTSVI
TWLVAVFASVPGIIFTKCQKEDSVYVCGPYFPRGWNNFHTIMRNILGLVLPLLIMVICY
SGILKTLLRCRNGLEEGGNVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIG
DGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSK
GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVT
TLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVN
RIELKGIDFKEDGNILGHKLEYNIDKKRHRAVRVIFTIMIVYFLFWTPYNIVILLNTFQ
EFFGLSNCESTSQLDQATQVTETLGMTHCCINPIIYAFVGEKFRRYLSVFFRKHITKRF
CKQCPVFYRETVDGVTSTNTPSTGEQEVSAGL"
misc_feature 823..825
/label=Y49A
misc_feature 1036..1038
/label=Y120A
CDS 1399..2121
/codon_start=1
/product="improved fluorescent protein-based calcium sensor
(Chen et al., 2013)"
/label=cpGFP
/translation="NVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPV
LLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEEL
FTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTY
GVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIEL
KGIDFKEDGNILGHKLEYN"
CDS 2122..2127
/codon_start=1
/label=Linker 2
/translation="ID"
CDS 2518..2577
/codon_start=1
/label=KGC
/translation="KSRITSEGEYIPLDQIDINV"
CDS 2578..2637
/codon_start=1
/label=KGC
/translation="KSRITSEGEYIPLDQIDINV"
CDS 2638..2697
/codon_start=1
/label=KGC
/translation="KSRITSEGEYIPLDQIDINV"
CDS 2704..2724
/codon_start=1
/label=ER2
/translation="FCYENEV"
polyA_signal 2850..2971
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2978..3433)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3460..3564
/gene="bla"
/label=AmpR promoter
promoter 3566..3923
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3774..3909
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3958..4752
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 4984..5031
/label=HSV TK poly(A) signal
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 5360..5948
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
661 gatccaccgg tcgccaccat gctgagcacc agcagaagca gattcatcag aaacaccaac
721 gagagcggcg aggaggtgac caccttcttc gactacgact acggcgcccc ctgccacaag
781 ttcgacgtga agcagatcgg cgcccagctg ctgccccccc tggccagcct ggtgttcatc
841 ttcggcttcg tgggcaacat gctggtggtg ctgatcctga tcaactgcaa gaagctgaag
901 tgcctgaccg acatctacct gctgaacctg gccatcagcg acctgctgtt cctgatcacc
961 ctgcccctgt gggcccacag cgccgccaac gagtgggtgt tcggcaacgc catgtgcaag
1021 ctgttcaccg gcctggccca catcggctac ttcggcggca tcttcttcat catcctgctg
1081 accatcgaca gatacctggc catcgtgcac gccgtgttcg ccctgaaggc cagaaccgtg
1141 accttcggcg tggtgaccag cgtgatcacc tggctggtgg ccgtgttcgc cagcgtgccc
1201 ggcatcatct tcaccaagtg ccagaaggag gacagcgtgt acgtgtgcgg cccctacttc
1261 cccagaggct ggaacaactt ccacaccatc atgagaaaca tcctgggcct ggtgctgccc
1321 ctgctgatca tggtgatctg ctacagcggc atcctgaaga ccctgctgag atgcagaaac
1381 ggcctggagg agggaggaaa cgtctatatc aaggccgaca agcagaagaa cggcatcaag
1441 gcgaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc ctaccactac
1501 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
1561 gtgcagtcca aactttcgaa agaccccaac gagaagcgcg atcacatggt cctgctggag
1621 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg cggtaccgga
1681 gggagcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
1741 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggtga gggcgatgcc
1801 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
1861 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
1921 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacatcca ggagcgcacc
1981 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
2041 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
2101 gggcacaagc tggagtacaa catcgacaag aagagacaca gagccgtgag agtgatcttc
2161 accatcatga tcgtgtactt cctgttctgg accccctaca acatcgtgat cctgctgaac
2221 accttccagg agttcttcgg cctgagcaac tgcgagagca ccagccagct ggaccaggcc
2281 acccaggtga ccgagaccct gggcatgacc cactgctgca tcaaccccat catctacgcc
2341 ttcgtgggcg agaagttcag aagatacctg agcgtgttct tcagaaagca catcaccaag
2401 agattctgca agcagtgccc cgtgttctac agagagaccg tggacggcgt gaccagcacc
2461 aacaccccca gcaccggcga gcaggaggtg agcgccggcc tgatggacga gctgtacaag
2521 agtagaatca caagcgaagg cgagtacatc cccctggatc aaatagacat aaatgtaaag
2581 agtagaatca caagcgaagg cgagtacatc cccctggatc aaatagacat aaatgtaaag
2641 agtagaatca caagcgaagg cgagtacatc cccctggatc aaatagacat aaatgtaggt
2701 ggattttgtt atgagaatga agtgtaaagc ggccgcgact ctagatcata atcagccata
2761 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
2821 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
2881 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
2941 gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg
3001 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
3061 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
3121 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
3181 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
3241 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
3301 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
3361 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
3421 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
3481 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
3541 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc
3601 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
3661 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
3721 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
3781 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
3841 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
3901 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg
3961 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
4021 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
4081 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
4141 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
4201 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
4261 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
4321 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
4381 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
4441 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
4501 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
4561 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
4621 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
4681 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
4741 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
4801 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
4861 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
4921 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
4981 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg
5041 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
5101 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
5161 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
5221 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
5281 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
5341 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
5401 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
5461 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
5521 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
5581 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
5641 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
5701 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
5761 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
5821 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
5881 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
5941 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
6001 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc
6061 at
//
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