| 质粒名称: | pWPXLd |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 12258 |
| 存储实验室: | Didier Trono |
|
载体骨架基本信息 |
|
| 载体名称: | pWPXLd |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 10455 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | EGFP |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | MCS - see map (未销毁) |
| 3' 克隆位点: | MCS - see map (未销毁) |
| 5' 测序引物 | See map |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pWPXLd was a gift from Didier Trono (Addgene plasmid # 12258 ; http://n2t.net/addgene:12258 ; RRID:Addgene_12258) |
买家导航
pWPXLd载体质粒基本信息
pWPXLd质粒图谱载体图谱和pWPXLd载体序列质粒序列多克隆位点信息
pWPXLd质粒载体简介
pWPXLd质粒序列载体序列
LOCUS pWPXLd 10455 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pWPXLd
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10455)
TITLE Trono Lab Constitutive Lentiviral Plasmids
REFERENCE 2 (bases 1 to 10455)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10455
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 2..635
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 682..807
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1300..1533
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
promoter 2120..3297
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2351..3288
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3245..3265
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
misc_feature 3345..3462
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
regulatory 3538..3547
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3544..4260
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(3589..3610)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3850..3869)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4197..4218
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 4340..4928
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4393..4413)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4811..4822)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5017..5250
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(5275..5293)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(5276..5293)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(5583..5602)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(5816..5835)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5935..5957
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(5995..6013)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6081..6185
/gene="bla"
/label=AmpR promoter
CDS 6186..7046
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6404..6423)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7217..7805
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7706..7725
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 7959..7976
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(8046..8066)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 8051..8380
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 8231..8366
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8293..8312
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 9514..9579
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 9709..9729
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 10154..10288
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10191..10210)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10245..10264
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt
2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt
2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg
3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttccgatcac gagactagcc
3481 tcgaggttta aactacggga tccaggccta agcttacgcg tcctagcgct accggtcgcc
3541 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
3601 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
3661 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
3721 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
3781 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
3841 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
3901 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
3961 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
4021 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
4081 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
4141 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
4201 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
4261 tccggactca gatctcgata gctagtagct agctagctag tcgagctcaa cttcgaattc
4321 ccgggactag tcatatgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
4381 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
4441 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
4501 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
4561 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
4621 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
4681 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
4741 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
4801 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
4861 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
4921 ctccccgcat cggtacgtat ggccaggtac ctttaagacc aatgacttac aaggcagctg
4981 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
5041 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
5101 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
5161 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
5221 ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata
5281 gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc
5341 taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc
5401 atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg
5461 taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca
5521 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
5581 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
5641 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
5701 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
5761 ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa
5821 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
5881 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
5941 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
6001 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
6061 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
6121 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
6181 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
6241 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
6301 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
6361 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
6421 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
6481 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
6541 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
6601 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
6661 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
6721 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
6781 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
6841 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
6901 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
6961 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
7021 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
7081 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
7141 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
7201 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
7261 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
7321 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
7381 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
7441 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
7501 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
7561 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
7621 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
7681 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
7741 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
7801 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
7861 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
7921 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
7981 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
8041 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
8101 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
8161 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
8221 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
8281 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
8341 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg
8401 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
8461 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
8521 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
8581 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
8641 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt
8701 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
8761 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
8821 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
8881 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
8941 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
9001 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
9061 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
9121 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
9181 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
9241 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
9301 ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
9361 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga
9421 tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
9481 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
9541 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
9601 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
9661 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
9721 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
9781 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
9841 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
9901 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
9961 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
10021 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
10081 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
10141 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
10201 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
10261 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
10321 caaacttccc accccatacc ctattaccac tgccaattac ctagtggttt catttactct
10381 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
10441 ctacaaactt agtag
//
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