| 质粒名称: | pDJ686 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001159 |
| 产品用途: | Mammalian STAT3-responsive SEAP expression vector |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | STAT3-responsive |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | NanoLuc |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Fluorescent protein-based ticker tapes for multiplexed recordings of transcriptional histories in single cells in culture and in vivo
Wang R., Jiang J., Li Z., Liu T., Wang Y., Xie M., Piatkevich K.D.
Preprint
|
pDJ686
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pDJ686载体质粒基本信息
pDJ686质粒图谱载体图谱和pDJ686载体序列质粒序列多克隆位点信息
pDJ686质粒载体简介
pDJ686质粒序列载体序列
LOCUS pDJ686 6566 bp DNA circular SYN 10-MAR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6566)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Aug 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6566
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 78..99
/label=oYW269
/note="LA-NheI-F"
misc_feature 196..226
/label=SleepingBeauty-ITR_Lo
/label=nonstandard type: ITR
misc_feature 392..423
/label=SleepingBeauty-ITR_Li
/label=nonstandard type: ITR
primer_bind 451..498
/label=oYW336
primer_bind 460..476
/label=oYW181
/note="P_hCMV-F"
primer_bind complement(460..476)
/label=oYW179
/note="ITR PCR F"
misc_feature 477..1580
/label=EF-1a
/note="/vntifkey=21"
primer_bind 477..498
/label=oYW338
/note="LA-NruI-PEF1a PCR-F"
promoter 493..1671
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
primer_bind 496..510
/label=oYW43
/note="LA-MluI-hEF1a PCR F"
primer_bind 496..510
/label=oYW183
/note="P_hEF1a PCR F"
primer_bind 604..627
/label=oDJ223-1
primer_bind 605..627
/label=oYW355
/note="LA-NruI-PEF1a PCR-F"
primer_bind 605..624
/label=oDJ8-1
primer_bind complement(611..630)
/label=EF1a-R
primer_bind complement(671..690)
/label=oYW45
/note="hEF1a mutant TOP motif LA-R"
primer_bind 673..713
/label=oYW46
/note="hEF1a mutant TOP motif RA-F"
intron 724..1662
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 937..956
/label=oYW361
/note="PEF1a mid seq primer"
primer_bind 1076..1093
/label=oYW47
/note="hEF1a-Seq-F"
primer_bind 1438..1459
/label=oYW285
/note="PEF1a-F for pYW136/137"
primer_bind complement(1551..1560)
/label=oYW346
primer_bind 1619..1639
/label=pEF-F/EF-1aFwd
primer_bind complement(1640..1659)
/label=oYW184
/note="P_hEF1a PCR R"
primer_bind complement(1643..1697)
/label=oYW337
primer_bind complement(1643..1672)
/label=oYW348
/note="RA-PEF1a PCR R"
primer_bind complement(1643..1672)
/label=oYW354
/note="RA-PEF1a PCR R"
primer_bind complement(1643..1671)
/label=oYW339
/note="RA-NheI-PEF1a PCR R"
primer_bind 1643..1659
/label=oYW180
/note="Puro PCR R"
primer_bind 1670..1707
/label=oYW333
/note="LA-AgeI-PacI-EcoRV-IRES PCR-F"
misc_feature 1730..2303
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(1776..1795)
/label=pIRES2-EGFP.P3
primer_bind complement(1825..1844)
/label=IRES-R
primer_bind 2301..2312
/label=oDJ199-3
primer_bind complement(2316..2361)
/label=oYW335
/note="RA-SalI-IRES PCR R"
primer_bind complement(2316..2350)
/label=oYW344
primer_bind complement(2316..2350)
/label=oYW345
primer_bind complement(2316..2335)
/label=oYW334
/note="IRES PCR R"
primer_bind 2336..2373
/label=oYW193
/note="LA-PuroR-F"
primer_bind 2351..2373
/label=oYW180
/note="Puro PCR R"
CDS 2353..2952
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(2838..2891)
/label=oYS113
primer_bind complement(2937..2952)
/label=oYW194
/note="PuroR-R"
primer_bind complement(2995..3012)
/label=BGH/pcDNA3.1R
primer_bind complement(2995..3012)
/label=pcDNA3.1-R(BGH-R)
primer_bind 2998..3017
/label=oYW188
/note="bGH poly A"
polyA_signal 3001..3225
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(3005..3022)
/label=oYW190
/note="BGH poly A R"
primer_bind complement(3067..3085)
/label=oYW303
/note="PolyA tail-bGH pA"
primer_bind complement(3067..3085)
/label=oYW57
/note="bGH seq primer R"
primer_bind complement(3070..3097)
/label=oYW300
/note="PolyA tail-bGH pA"
primer_bind complement(3070..3093)
/label=oYW302
/note="bGH pA R"
primer_bind 3070..3089
/label=W2F0627-P099-D03
/note="Sequencing primer"
primer_bind 3128..3147
/label=BGH-polyA-R
primer_bind 3208..3254
/label=oYW203
/note="LA-P_CMV-F"
primer_bind complement(3208..3231)
/label=oYW202
/note="BGH polyA-R"
primer_bind complement(3208..3225)
/label=oYW56
/note="LA-BamHI-PPT-MluI-bGH pA PCR"
primer_bind complement(3208..3225)
/label=oYW53
/note="LA-BamHI-bGH pA"
primer_bind complement(3209..3226)
/label=oYW282
/note="Arm-PCR pYW134-R"
primer_bind 3226..3254
/label=oYW189
/note="LA-P_CMV-F"
primer_bind 3245..3262
/label=oYW90
/note="LA-AgeI-PBS-PCMV F"
primer_bind 3246..3271
/label=oYW355
/note="LA-NruI-PEF1a PCR-F"
primer_bind 3254..3270
/label=oYW326
primer_bind 3274..3317
/label=oDJ656-1
primer_bind 3274..3294
/label=oDJ686-1
primer_bind 3274..3293
/label=oDJ223-1
primer_bind 3274..3292
/label=oYW278
/note="LA-NotI-P_GFAP-F"
primer_bind 3274..3292
/label=oYW181
/note="P_hCMV-F"
primer_bind 3294..3317
/label=oDJ584-1
misc_feature 3326..3426
/label=4x STAT binding
misc_feature 3326..3340
/label=STAT3 binding motif
misc_feature 3350..3369
/label=STAT3 binding motif
misc_feature 3375..3394
/label=STAT3 binding motif
misc_feature 3378..3386
/label=TTCNNNgAA
misc_feature 3400..3419
/label=STAT3 binding motif
misc_feature 3427..3471
/label=P_CMVmin
promoter 3432..3470
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 3515..3533
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 3527..3546
/label=oYW346
primer_bind complement(3536..3555)
/label=oDJ584-2
primer_bind 3536..3555
/label=oDJ584-3
primer_bind complement(3544..3581)
/label=oDJ686-2
primer_bind 3562..3594
/label=oQZ280
primer_bind 3562..3583
/label=oDJ684-3
primer_bind 3562..3579
/label=oQZ321
regulatory 3562..3570
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3568..3636
/codon_start=1
/label=Secretory (IgK)
/label=nonstandard type: signal
/translation="MTSETDTLLLWVLLLWVPGSTGD"
primer_bind 3568..3594
/label=oQZ331
CDS 3568..3570
/codon_start=1
/label=mChilada
/translation="M"
primer_bind 3571..3592
/label=oQZ757
primer_bind complement(3638..3670)
/label=oQZ758
primer_bind 3638..3670
/label=oQZ759
CDS 3655..4170
/codon_start=1
/product="NanoLuc(R) luciferase"
/label=Nluc
/note="human codon-optimized"
/translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
RLCERILA"
primer_bind complement(3765..3788)
/label=oQZ826
primer_bind 3789..3813
/label=oQZ827
primer_bind complement(4149..4190)
/label=oDJ684-4
primer_bind complement(4149..4170)
/label=oQZ281
primer_bind complement(4149..4170)
/label=oDJ657-2
primer_bind complement(4152..4170)
/label=oQZ322
primer_bind complement(4190..4207)
/label=BGH/pcDNA3.1R
primer_bind complement(4190..4207)
/label=pcDNA3.1-R(BGH-R)
primer_bind 4193..4212
/label=oYW188
/note="bGH poly A"
polyA_signal 4196..4420
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(4200..4217)
/label=oYW190
/note="BGH poly A R"
primer_bind complement(4262..4280)
/label=oYW303
/note="PolyA tail-bGH pA"
primer_bind complement(4262..4280)
/label=oYW57
/note="bGH seq primer R"
primer_bind complement(4265..4292)
/label=oYW300
/note="PolyA tail-bGH pA"
primer_bind complement(4265..4288)
/label=oYW302
/note="bGH pA R"
primer_bind 4265..4284
/label=W2F0627-P099-D03
/note="Sequencing primer"
primer_bind 4323..4342
/label=BGH-polyA-R
primer_bind 4403..4421
/label=oYW203
/note="LA-P_CMV-F"
primer_bind complement(4403..4421)
/label=oYW202
/note="BGH polyA-R"
primer_bind complement(4403..4420)
/label=oYW56
/note="LA-BamHI-PPT-MluI-bGH pA PCR"
primer_bind complement(4403..4420)
/label=oYW53
/note="LA-BamHI-bGH pA"
primer_bind complement(4404..4421)
/label=oYW282
/note="Arm-PCR pYW134-R"
primer_bind 4431..4447
/label=oYW183
/note="P_hEF1a PCR F"
primer_bind 4432..4453
/label=oYW197
/note="ITR PCR F1"
primer_bind complement(4463..4478)
/label=oYW336
misc_feature complement(4504..4532)
/label=SleepingBeauty-ITR_Ri
/label=nonstandard type: ITR
misc_feature 4698..4729
/label=SleepingBeauty-ITR-Ro
/label=nonstandard type: ITR
primer_bind complement(4872..4891)
/label=oYW207
/note="pLL3.7 PCR R"
primer_bind complement(4873..4898)
/label=oYW301
/note="PolyA tail-pLL3.7 3'LTR"
rep_origin complement(4917..5505)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5056..5075)
/label=W5CF0703-P099-D09
/note="Sequencing primer"
CDS complement(5676..6536)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5693..5712)
/label=W4CF0701-P099-E08
/note="Sequencing primer"
primer_bind complement(6357..6376)
/label=W3CF0630-P099-D08
/note="Sequencing primer"
primer_bind complement(6413..6432)
/label=W3CF0630-P099-A08
/note="Sequencing primer"
primer_bind complement(6413..6432)
/label=W3CF0630-P099-F07
/note="Sequencing primer"
primer_bind 6507..6526
/label=oYW24
/note="Forward seq primer of AmpR"
promoter complement(join(6537..6566,1..75))
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
61 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gatgcggtgt gaaataccgc
121 acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa
181 attggatccc tatacagttg aagtcggaag tttacataca cttaagttgg agtcattaaa
241 actcgttttt caactactcc acaaatttct tgttaacaaa caatagtttt ggcaagtcag
301 ttaggacatc tactttgtgc atgacacaag tcatttttcc aacaattgtt tacagacaga
361 ttatttcact tataattcac tgtatcacaa ttccagtggg tcagaagttt acatacacta
421 agttcgactc ctctgcagaa tgcggcgatg tttcggtaag gggtccgcta gaattcgaaa
481 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
541 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
601 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
661 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
721 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
781 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
841 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
901 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
961 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
1021 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
1081 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
1141 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
1201 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
1261 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
1321 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
1381 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
1441 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
1501 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
1561 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
1621 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg aaccggttta
1681 attaagatat ctgcatctag ggcggccaat tccgcccctc tcccccccgc ccctctccct
1741 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
1801 atatgtgatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
1861 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
1921 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
1981 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
2041 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
2101 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
2161 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
2221 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
2281 tcctttgaaa aacacgatga taagcttgcc acaacccaca aggagacgac cttccgtcga
2341 cgccaccatg ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
2401 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
2461 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
2521 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
2581 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
2641 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
2701 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
2761 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
2821 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
2881 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
2941 cccggtgcct gattcgaagg cctgtcgtgg gggatcaatt cgctcgctga tcagcctcga
3001 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
3061 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
3121 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
3181 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcctag gagaatctgc
3241 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtcatgctc
3301 taggaagatc tccacgcgtc tcgagatttc ccgtaaatcg tcgagtggac atttcccgta
3361 aatcgtcgag tggacatttc ccgtaaatcg tcgagtggac atttcccgta aatcgtcgag
3421 ctgcagtcga gggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa
3481 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
3541 aagctgttcg aagcggaatt cgccaccatg actagtgaga cagacacact cctgctatgg
3601 gtactgctgc tctgggttcc aggttccact ggtgacgcta gtggtggttc tggtatggtc
3661 ttcacactcg aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa
3721 gtccttgaac agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg
3781 atccaaagga ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc
3841 ccgtatgaag gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg
3901 taccctgtgg atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac
3961 ggggttacgc cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc
4021 gacggcaaaa agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag
4081 cgcctgatca accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc
4141 tggcggctgt gcgaacgcat tctggcgtga tctagagctc gctgatcagc ctcgactgtg
4201 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
4261 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
4321 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
4381 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
4441 agctgggcct actagctact cgggacccct taccgaaaca tcgccgcatt ctgcagagga
4501 gtcgagtgta tgtaaacttc tgacccactg ggaatgtgat gaaagaaata aaagctgaaa
4561 tgaatcattc tctctactat tattctgata tttcacattc ttaaaataaa gtggtgatcc
4621 taactgacct aagacaggga atttttacta ggattaaatg tcaggaattg tgaaaaagtg
4681 agtttaaatg tatttggcta aggtgtatgt aaacttccga cttcaactgt atagggatcc
4741 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
4801 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
4861 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
4921 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
4981 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
5041 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
5101 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
5161 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
5221 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
5281 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
5341 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
5401 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
5461 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
5521 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
5581 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
5641 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
5701 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
5761 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
5821 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
5881 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
5941 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
6001 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
6061 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
6121 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
6181 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
6241 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
6301 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
6361 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
6421 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
6481 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
6541 ttcctttttc aatattattg aagcat
//
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