px330-U6-AsCas12a gRNA(Bbs1)-CAG-AsCpf1-P2A-mCherry

LOCUS      px330-U6-AsCas12a gRNA(Bbs1)-CAG-AsCpf1-P2A-mCherry               8841 bp DNA     circular SYN 20-APR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        651..899
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     enhancer        959..1244
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        1246..1522
                     /label=chicken beta-actin promoter
                     /note="contains a 21-bp deletion relative to the standard 
                     chicken beta-actin promoter"
     promoter        1367..1386
                     /label=chicken beta-actin promoter
                     /note="contains a 21-bp deletion relative to the standard 
                     chicken beta-actin promoter"
     intron          1523..1750
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             1762..5682
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     misc_feature    1874..1879
                     /label=SDS
     misc_feature    2474..2479
                     /label=SDS
     misc_feature    3143..3148
                     /label=SDS
     misc_feature    4316..4321
                     /label=SDS
     misc_feature    4910..4915
                     /label=SDS
     regulatory      5402..5411
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5683..5730
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5740..5796
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5797..6507
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    5813..5818
                     /label=SDS
     misc_feature    6245..6250
                     /label=SDS
     polyA_signal    6538..6745
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6754..6894
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    6754..6883
                     /label=AAV2 ITR
     rep_origin      6969..7424
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7706..7810
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7811..8671
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt gagggcctat
      661 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa
      721 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
      781 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
      841 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
      901 taatttctac tcttgtagat atcggatatg gtaagacatg tttttttcta gaggtacccg
      961 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     1021 cgtcaatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1081 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1141 tcaatgacgg taaatggccc gcctggcatt gtgcccagta catgacctta tgggactttc
     1201 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     1261 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     1321 ttttttaatt attttgtgca gcgatggggg cggggggggg ggggggcgcg cgccaggcgg
     1381 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca
     1441 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
     1501 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc
     1561 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
     1621 cgggcgggac ggcccttctc ctccgggctg taattagctg agcaagaggt aagggtttaa
     1681 gggatggttg gttggtgggg tattaatgtt taattacctg gagcacctgc ctgaaatcac
     1741 tttttttcag gttggaccgg tatgacacag ttcgagggct ttaccaacct gtatcaggtg
     1801 agcaagacac tgcggtttga gctgatccca cagggcaaga ccctgaagca catccaggag
     1861 cagggcttca tcgaggagga caaggcccgc aatgatcact acaaggagct gaagcccatc
     1921 atcgatcgga tctacaagac ctatgccgac cagtgcctgc agctggtgca gctggattgg
     1981 gagaacctga gcgccgccat cgactcctat agaaaggaga aaaccgagga gacaaggaac
     2041 gccctgatcg aggagcaggc cacatatcgc aatgccatcc acgactactt catcggccgg
     2101 acagacaacc tgaccgatgc catcaataag agacacgccg agatctacaa gggcctgttc
     2161 aaggccgagc tgtttaatgg caaggtgctg aagcagctgg gcaccgtgac cacaaccgag
     2221 cacgagaacg ccctgctgcg gagcttcgac aagtttacaa cctacttctc cggcttttat
     2281 gagaacagga agaacgtgtt cagcgccgag gatatcagca cagccatccc acaccgcatc
     2341 gtgcaggaca acttccccaa gtttaaggag aattgtcaca tcttcacacg cctgatcacc
     2401 gccgtgccca gcctgcggga gcactttgag aacgtgaaga aggccatcgg catcttcgtg
     2461 agcacctcca tcgaggaggt gttttccttc cctttttata accagctgct gacacagacc
     2521 cagatcgacc tgtataacca gctgctggga ggaatctctc gggaggcagg caccgagaag
     2581 atcaagggcc tgaacgaggt gctgaatctg gccatccaga agaatgatga gacagcccac
     2641 atcatcgcct ccctgccaca cagattcatc cccctgttta agcagatcct gtccgatagg
     2701 aacaccctgt ctttcatcct ggaggagttt aagagcgacg aggaagtgat ccagtccttc
     2761 tgcaagtaca agacactgct gagaaacgag aacgtgctgg agacagccga ggccctgttt
     2821 aacgagctga acagcatcga cctgacacac atcttcatca gccacaagaa gctggagaca
     2881 atcagcagcg ccctgtgcga ccactgggat acactgagga atgccctgta tgagcggaga
     2941 atctccgagc tgacaggcaa gatcaccaag tctgccaagg agaaggtgca gcgcagcctg
     3001 aagcacgagg atatcaacct gcaggagatc atctctgccg caggcaagga gctgagcgag
     3061 gccttcaagc agaaaaccag cgagatcctg tcccacgcac acgccgccct ggatcagcca
     3121 ctgcctacaa ccctgaagaa gcaggaggag aaggagatcc tgaagtctca gctggacagc
     3181 ctgctgggcc tgtaccacct gctggactgg tttgccgtgg atgagtccaa cgaggtggac
     3241 cccgagttct ctgcccggct gaccggcatc aagctggaga tggagccttc tctgagcttc
     3301 tacaacaagg ccagaaatta tgccaccaag aagccctact ccgtggagaa gttcaagctg
     3361 aactttcaga tgcctacact ggcctctggc tgggacgtga ataaggagaa gaacaatggc
     3421 gccatcctgt ttgtgaagaa cggcctgtac tatctgggca tcatgccaaa gcagaagggc
     3481 aggtataagg ccctgagctt cgagcccaca gagaaaacca gcgagggctt tgataagatg
     3541 tactatgact acttccctga tgccgccaag atgatcccaa agtgcagcac ccagctgaag
     3601 gccgtgacag cccactttca gacccacaca acccccatcc tgctgtccaa caatttcatc
     3661 gagcctctgg agatcacaaa ggagatctac gacctgaaca atcctgagaa ggagccaaag
     3721 aagtttcaga cagcctacgc caagaaaacc ggcgaccaga agggctacag agaggccctg
     3781 tgcaagtgga tcgacttcac aagggatttt ctgtccaagt ataccaagac aacctctatc
     3841 gatctgtcta gcctgcggcc atcctctcag tataaggacc tgggcgagta ctatgccgag
     3901 ctgaatcccc tgctgtacca catcagcttc cagagaatcg ccgagaagga gatcatggat
     3961 gccgtggaga caggcaagct gtacctgttc cagatctata acaaggactt tgccaagggc
     4021 caccacggca agcctaatct gcacacactg tattggaccg gcctgttttc tccagagaac
     4081 ctggccaaga caagcatcaa gctgaatggc caggccgagc tgttctaccg ccctaagtcc
     4141 aggatgaaga ggatggcaca ccggctggga gagaagatgc tgaacaagaa gctgaaggat
     4201 cagaaaaccc caatccccga caccctgtac caggagctgt acgactatgt gaatcacaga
     4261 ctgtcccacg acctgtctga tgaggccagg gccctgctgc ccaacgtgat caccaaggag
     4321 gtgtctcacg agatcatcaa ggataggcgc tttaccagcg acaagttctt tttccacgtg
     4381 cctatcacac tgaactatca ggccgccaat tccccatcta agttcaacca gagggtgaat
     4441 gcctacctga aggagcaccc cgagacacct atcatcggca tcgatcgggg cgagagaaac
     4501 ctgatctata tcacagtgat cgactccacc ggcaagatcc tggagcagcg gagcctgaac
     4561 accatccagc agtttgatta ccagaagaag ctggacaaca gggagaagga gagggtggca
     4621 gcaaggcagg cctggtctgt ggtgggcaca atcaaggatc tgaagcaggg ctatctgagc
     4681 caggtcatcc acgagatcgt ggacctgatg atccactacc aggccgtggt ggtgctggag
     4741 aacctgaatt tcggctttaa gagcaagagg accggcatcg ccgagaaggc cgtgtaccag
     4801 cagttcgaga agatgctgat cgataagctg aattgcctgg tgctgaagga ctatccagca
     4861 gagaaagtgg gaggcgtgct gaacccatac cagctgacag accagttcac ctcctttgcc
     4921 aagatgggca cccagtctgg cttcctgttt tacgtgcctg ccccatatac atctaagatc
     4981 gatcccctga ccggcttcgt ggaccccttc gtgtggaaaa ccatcaagaa tcacgagagc
     5041 cgcaagcact tcctggaggg cttcgacttt ctgcactacg acgtgaaaac cggcgacttc
     5101 atcctgcact ttaagatgaa cagaaatctg tccttccaga ggggcctgcc cggctttatg
     5161 cctgcatggg atatcgtgtt cgagaagaac gagacacagt ttgacgccaa gggcacccct
     5221 ttcatcgccg gcaagagaat cgtgccagtg atcgagaatc acagattcac cggcagatac
     5281 cgggacctgt atcctgccaa cgagctgatc gccctgctgg aggagaaggg catcgtgttc
     5341 agggatggct ccaacatcct gccaaagctg ctggagaatg acgattctca cgccatcgac
     5401 accatggtgg ccctgatccg cagcgtgctg cagatgcgga actccaatgc cgccacaggc
     5461 gaggactata tcaacagccc cgtgcgcgat ctgaatggcg tgtgcttcga ctcccggttt
     5521 cagaacccag agtggcccat ggacgccgat gccaatggcg cctaccacat cgccctgaag
     5581 ggccagctgc tgctgaatca cctgaaggag agcaaggatc tgaagctgca gaacggcatc
     5641 tccaatcagg actggctggc ctacatccag gagctgcgca acaaaaggcc ggcggccacg
     5701 aaaaaggccg gccaggcaaa aaagaaaaag ggaagcggag ctactaactt cagcctgctg
     5761 aagcaggctg gagacgtgga ggagaaccct ggacctatgg tgagcaaggg cgaggaggat
     5821 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     5881 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     5941 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     6001 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     6061 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga tgacggcggc
     6121 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     6181 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaaaac catgggctgg
     6241 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     6301 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     6361 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     6421 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     6481 ggcggcatgg acgagctgta caagtaagaa ttcctagagc tcgctgatca gcctcgactg
     6541 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     6601 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     6661 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     6721 aagagaatag caggcatgct ggggagcggc cgcaggaacc cctagtgatg gagttggcca
     6781 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     6841 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     6901 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     6961 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     7021 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
     7081 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     7141 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     7201 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     7261 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
     7321 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
     7381 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     7441 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     7501 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     7561 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     7621 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     7681 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7741 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7801 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7861 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7921 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7981 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8041 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8101 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8161 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     8221 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8281 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8341 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8401 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8461 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8521 gcgtggaagc cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8581 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8641 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8701 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8761 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8821 agaaaagatc aaaggatctt c
//