pSL1517

LOCUS       pSL1517             7911 bp DNA     circular SYN 22-JUL-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     213..252
                     /label=oSCL2112
     misc_feature    232
                     /label=CMV
     misc_feature    233..716
                     /label=RSV promoter
                     /note="RSV promoter"
     primer_bind     301..321
                     /label=oSCL2112
     misc_feature    745..811
                     /label=Exon1
                     /note="Exon1"
     misc_feature    812..1011
     misc_feature    1012..1070
                     /label=PSEx
                     /note="PSEx"
     misc_feature    1039..1047
                     /label=KozacATG
                     /note="KozacATG"
     misc_feature    1071..1270
     misc_feature    1271..1305
                     /label=Exon2
                     /note="Exon2"
     primer_bind     complement(1297..1336)
                     /label=oSCL2183
     primer_bind     complement(1297..1316)
                     /label=oSCL2113
     primer_bind     1297..1316
                     /label=oSCL2116
     primer_bind     complement(1316..1336)
                     /label=oSCL1109
     misc_feature    1317..1376
                     /label=P2A
     primer_bind     complement(1356..1376)
                     /label=oSCL2238
     primer_bind     1357..1415
                     /label=oSCL2185
     primer_bind     1371..1415
                     /label=oSCL2116
     misc_feature    1377..2930
                     /label=SEAP
     primer_bind     complement(1562..1598)
                     /label=oSCL545
     primer_bind     1579..1607
                     /label=oSCL544
     primer_bind     complement(2395..2426)
                     /label=oSCL546
     primer_bind     2407..2439
                     /label=oSCL547
     primer_bind     2492..2522
                     /label=Oscl50-1
     primer_bind     complement(2492..2522)
                     /label=Oscl50-2
     primer_bind     complement(2881..2930)
                     /label=oSCL795
     primer_bind     complement(2894..2943)
                     /label=oSCL2520
     primer_bind     complement(2894..2930)
                     /label=Primer 1
     primer_bind     complement(2915..2935)
                     /label=oSCL475
     primer_bind     complement(2917..2930)
                     /label=Oscl31-4
     primer_bind     complement(2917..2930)
                     /label=oSCL457
     primer_bind     complement(2917..2930)
                     /label=oSCL548
     primer_bind     complement(2917..2930)
                     /label=oSCL776
     primer_bind     complement(2917..2930)
                     /label=oSCL083
     primer_bind     complement(2917..2930)
                     /label=oSCL2117
     primer_bind     2924..2981
                     /label=oSCL2519
     polyA_signal    2925..2930
                     /label=SV40 late polyA
     misc_feature    2961..3470
                     /label=NLuc (nano Luciferase)
                     /label=nonstandard type:  reporter
     misc_feature    2991..2993
                     /label=cyt-STOP (TGG->TGA)
     primer_bind     complement(3450..3493)
                     /label=Nluc-2
     primer_bind     complement(3450..3473)
                     /label=oSCL1849
     primer_bind     complement(3460..3470)
                     /label=oSCL085
     primer_bind     complement(3460..3470)
                     /label=oSCL1109
     primer_bind     3474..3493
                     /label=oSCL2184
     primer_bind     complement(3474..3493)
                     /label=oSCL2117
     misc_feature    3474
                     /label=SEAP
     polyA_signal    3474
                     /label=SV40 late polyA
     terminator      3508..3735
                     /label=bGH PA
     misc_feature    4403..4480
                     /label=SV40 ORI
     CDS             4622..5413
                     /label=Kan/neoR
     primer_bind     complement(5753..5773)
                     /label=M13-rev
     misc_binding    complement(5779..5801)
                     /label=LacO
     promoter        complement(5806..5835)
                     /label=lac
     CDS             complement(6918..7577)
                     /label=AmpR
     promoter        complement(7817..7845)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtcgagcct
      241 gcaggcgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
      301 gtacgggcca gatatacgcg tatctgaggg gactagggtg tgtttaggcg aaaagcgggg
      361 cttcggttgt acgcggttag gagtcccctc aggatatagt agtttcgctt ttgcataggg
      421 agggggaaat gtagtcttat gcaatacact tgtagtcttg caacatggta acgatgagtt
      481 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
      541 ggtacgatcg tgccttatta ggaaggcaac agacaggtct gacatggatt ggacgaacca
      601 ctgaattgcg cattgcagag ataattgtat ttaagtgcct agctcgatac aataaacgcc
      661 atttgaccat tcaccacatt ggtgtgcacc tccaaggcca cagatccgtt taaacaagct
      721 tggcaatccg gtactgttgg taaatttctg tacaacttaa ccttgcagag agccactggc
      781 atcagctttg ccattcttgg aaacttttct ggtaagttct ctcgttacca tcttttgaaa
      841 ttttaagtga attaatacat atcttgctta gtctcttgtg caggaaatgt tttccattta
      901 tgacaaaaca ggaattgtgt gaaatttcaa atattggatt aggaaataca aagttactga
      961 aagtgaggta ctaatgttta taaaataaaa actttttctt gccatttgca gatttaacat
     1021 ttttgagtca atccaagtgc caccatgcag gaggttcatg attgtgtaga gtaagacata
     1081 attttgttga ggtttaactc tgaatactta atgtggtact gaatacttaa tgtggtactg
     1141 agaggcagcc taactgacca cacagcattc acgcctggct aatttttgta tttttagtag
     1201 agacggggtt tcaccatggt ggccaggctg ggttctggtt gtttatgatc tttatttttt
     1261 ggtgatctag gaaccaaaca acaagaaatt gttgtttccc gtgggtaaga ggatccggag
     1321 caaccaactt ttccctgctg aagcaggcag gcgacgtgga ggagaatcct ggacccctgc
     1381 tgctgctgct gctgctgggc ctgaggctac agctctccct gggcatcatc ccagttgagg
     1441 aggagaaccc ggacttctgg aaccgcgagg cagccgaggc cctgggtgcc gccaagaagc
     1501 tgcagcctgc acagacagcc gccaagaacc tcatcatctt cctgggcgat gggatggggg
     1561 tgtctacggt gacagctgcc agaatcctaa aagggcagaa gaaggacaaa ctggggcctg
     1621 agatacccct ggccatggac cgcttcccat atgtggctct gtccaagaca tacaatgtag
     1681 acaaacatgt gccagacagt ggagccacag ccacggccta cctgtgcggg gtcaagggca
     1741 acttccagac cattggcttg agtgcagccg cccgctttaa ccagtgcaac acgacacgcg
     1801 gcaacgaggt catctccgtg atgaatcggg ccaagaaagc agggaagtca gtgggagtgg
     1861 taaccaccac acgagtgcag cacgcctcgc cagccggcac ctacgcccac acggtgaacc
     1921 gcaactggta ctcggacgcc gacgtgcctg cctcggcccg ccaggagggg tgccaggaca
     1981 tcgctacgca gctcatctcc aacatggaca ttgacgtgat cctaggtgga ggccgaaagt
     2041 acatgtttcg catgggaacc ccagaccctg agtacccaga tgactacagc caaggtggga
     2101 ccaggctgga cgggaagaat ctggtgcagg aatggctggc gaagcgccag ggtgcccggt
     2161 atgtgtggaa ccgcactgag ctcatgcagg cttccctgga cccgtctgtg acccatctca
     2221 tgggtctctt tgagcctgga gacatgaaat acgagatcca ccgagactcc acactggacc
     2281 cctccctgat ggagatgaca gaggctgccc tgcgcctgct gagcaggaac ccccgcggct
     2341 tcttcctctt cgtggagggt ggtcgcatcg accatggtca tcatgaaagc agggcttacc
     2401 gggcactgac tgagaccatc atgttcgacg acgccattga gagggcgggc cagctcacca
     2461 gcgaggagga cacgctgagc ctcgtcactg ccgaccactc ccacgtgttc tccttcggag
     2521 gctaccccct gcgagggagc tccatcttcg ggctggcccc tggcaaggcc cgggacagga
     2581 aggcctacac ggtcctccta tacggaaacg gtccaggcta tgtgctcaag gacggcgccc
     2641 ggccggatgt taccgagagc gagagcggga gccccgagta tcggcagcag tcagcagtgc
     2701 ccctggacga agagacccac gcaggcgagg acgtggcggt gttcgcgcgc ggcccgcagg
     2761 cgcacctggt tcacggcgtg caggagcaga ccttcatagc gcacgtcatg gccttcgccg
     2821 cctgcctgga gccctacacc gcctgcgacc tggcgccccc cgccggcacc accgacgccg
     2881 cgcacccggg ttacagcaga gtcggggcgg ccggccgctt cgagcagaca gccagcggaa
     2941 gtggcggagg aggcgacgtc gtcttcacac tcgaagattt cgttggggac tggcgacaga
     3001 cagccggcta caacctggac caagtccttg aacagggagg tgtgtccagt ttgtttcaga
     3061 atctcggggt gtccgtaact ccgatccaaa ggattgtcct gagcggtgaa aatgggctga
     3121 agatcgacat ccatgtcatc atcccgtatg aaggtctgag cggcgaccaa atgggccaga
     3181 tcgaaaaaat ttttaaggtg gtgtaccctg tggatgatca tcactttaag gtgatcctgc
     3241 actatggcac actggtaatc gacggggtta cgccgaacat gatcgactat ttcggacggc
     3301 cgtatgaagg catcgccgtg ttcgacggca aaaagatcac tgtaacaggg accctgtgga
     3361 acggcaacaa aattatcgac gagcgcctga tcaaccccga cggctccctg ctgttccgag
     3421 taaccatcaa cggagtgacc ggctggcggc tgtgcgaacg cattctggcg tgaactagag
     3481 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     3541 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     3601 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     3661 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     3721 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     3781 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     3841 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     3901 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     3961 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     4021 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4081 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4141 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4201 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     4261 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     4321 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     4381 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4441 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     4501 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     4561 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     4621 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     4681 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     4741 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     4801 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     4861 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     4921 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     4981 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     5041 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     5101 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     5161 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     5221 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     5281 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     5341 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     5401 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     5461 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     5521 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     5581 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5641 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5701 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     5761 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     5821 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     5881 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     5941 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6001 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6061 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6121 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6181 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6241 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6301 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6361 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6421 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6481 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6541 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6601 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6661 tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt
     6721 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6781 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6841 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6901 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6961 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7021 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7081 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7141 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7201 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7261 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7321 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7381 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7441 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7501 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     7561 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7621 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7681 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     7741 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     7801 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     7861 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//