JME4390

LOCUS       JME4390               12297 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  LEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA 
            cloning using GoldenGate.
ACCESSION   .
VERSION     .
KEYWORDS    JME4390
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12297)
  AUTHORS   Larroude M, Trabelsi H, Nicaud JM, Rossignol T
  TITLE     A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting 
            wild-type strain diversity.
  JOURNAL   Biotechnol Lett. 2020 Jan 23. pii: 10.1007/s10529-020-02805-4. doi: 
            10.1007/s10529-020-02805-4.
  PUBMED    31974649
REFERENCE   2  (bases 1 to 12297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12297
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      12..69
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(93..110)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(264..852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(344..363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1023..1883)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1646..1665
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1884..1988)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      2078..2121
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     protein_bind    2700..2721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2736..2766
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2774..2790
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2808..2819
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2826..3503
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      3548..3619
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3635..3662
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    4007..4040
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     complement(5910..5932)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6032..6051
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6245..6267
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6259..6276
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6260..6276
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             7889..11992
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             12005..12025
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      12036..12283
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(12052..12070)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 tcttcctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
       61 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
      121 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      181 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      241 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
      301 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      361 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      421 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      481 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      541 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      601 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      661 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
      721 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      781 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
      841 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
      901 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
      961 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1021 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1081 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1141 cccagtgctg caatgatacc gcgcgaccca cgctcaccgg ctccagattt atcagcaata
     1201 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1261 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1321 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1381 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1441 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1501 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1561 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     1621 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     1681 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     1741 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     1801 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     1861 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     1921 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     1981 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     2041 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
     2101 gctttcgcta aggatgattt ctggaattcg aagagcctct gacctgacgt ccccagttgc
     2161 aaaagttgac acaactctag atctgcttcc aaatatagaa tcataacaag ggttagggtg
     2221 tgattatata atattggtct taattgatgt gctagggctt taaaagttgg ttaaaataac
     2281 gctctaatgc ctttttaata tattgtcttt ttcaaaatct caaatcggac acttcttcgt
     2341 gtatgagact ccattttttg gctccgtcac gtgatatgta ttatcagcta tagtggtgta
     2401 aacaaagttt tttactagct gtaatggcat tttgtcggag tggtaaatcg ccttcttgtt
     2461 gtgcgttcga gttctggact ctgcactggg ctactttgaa aaatacctct aatgcgccga
     2521 tggtttagtg gtaaaatcca tcgttgccat cgatgggccc ccggttcgag agacggggcc
     2581 ggccatctgg gccctagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2641 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2701 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     2761 atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat
     2821 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
     2881 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
     2941 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
     3001 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
     3061 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
     3121 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
     3181 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
     3241 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag
     3301 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
     3361 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
     3421 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag
     3481 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc
     3541 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     3601 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     3661 cgtttatata ggccatctgg gccgggggcg tctctgagct agaaatagca agttaaaata
     3721 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tttacgtcta
     3781 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcccagatc
     3841 ctctagagtc gaagcggccg ctatgtctga taaaaggatg taacataggc aagctgctcg
     3901 tgagtgttga gtacgaacct tagatccaaa tcacccgcac ccacggatat acttgcttga
     3961 atatacagta gtatgcggcc gctaggtatt accctgttat ccctacataa cttcgtatag
     4021 catacattat acgaagttat tctgaattcc gcctgagtca tcatttattt accagttggc
     4081 cacaaaccct tgacgatctc gtatgtcccc tccgacatac tcccggccgg ctgggtacgt
     4141 tcgatagcgc tatcggcatc gacaaggttt gggtccctag ccgataccgc actacctgag
     4201 tcacaatctt cggaggttta gtcttccaca tagcacgggc aaaagtgcgt atatatacaa
     4261 gagcgtttgc cagccacaga ttttcactcc acacaccaca tcacacatac aaccacacac
     4321 atccacaatg gaacccgaaa ctaagaagac caagactgac tccaagaaga ttgttcttct
     4381 cggcggcgac ttctgtggcc ccgaggtgat tgccgaggcc gtcaaggtgc tcaagtctgt
     4441 tgctgaggcc tccggcaccg agtttgtgtt cgaggaccga ctcattggag gagctgccat
     4501 tgagaaggag ggcgagccca tcaccgacgc tactctcgac atctgccgaa aggctgactc
     4561 tattatgctc ggtgctgtcg gaggcgctgc caacaccgta tggaccactc ccgacggacg
     4621 aaccgacgtg cgacccgagc agggcctcct caagctgcga aaggacctga acctgtacgc
     4681 caacctgcga ccctgccagc tgctgtcgcc caagctcgcc gatctctccc ccatccgaaa
     4741 cgttgagggc accgacttca tcattgtccg agagctcgtc ggaggtatct actttggaga
     4801 gcgaaaggag gatgacggat ctggcgtcgc ttccgacacc gagacttact ccgttcctga
     4861 ggttgagcga attgcccgaa tggccgcctt cctggccctt cagcacaacc cccctcttcc
     4921 cgtgtggtcc cttgacaagg ccaacgtgct ggcctcctct cgactttggc gaaagactgt
     4981 cacccgagtc ctcaaggacg agttccccca gctggagctc aaccaccagc tgatcgactc
     5041 ggccgccatg atcctcatca agcagccctc caagatgaat ggtatcatca tcaccaccaa
     5101 catgtttggc gatatcatct ccgacgaggc ctccgtcatc cccggttctc tgggtctgct
     5161 gccctccgcc tctctggctt ctctgcccga caccaacgag gcgttcggtc tgtacgagcc
     5221 ctgtcacgga tctgcccccg atctcggcaa gcagaaggtc aaccccattg ccaccattct
     5281 gtctgccgcc atgatgctca agttctctct taacatgaag cccgccggtg acgctgttga
     5341 ggctgccgtc aaggagtccg tcgaggctgg tatcactacc gccgatatcg gaggctcttc
     5401 ctccacctcc gaggtcggag actttgttgc caacaaggtc aaggagctgc tcaagaagga
     5461 gtaagtcgtt tctacgacgc attgatggaa ggagcaaact gacgcgcctg cgggttggtc
     5521 taccggcagc agccctgcta atgaaatgat gatttataat ttaccggtgt agcaaccttg
     5581 actagaagaa gcagattggg tgtgtttgta gtggaggaca gtggtacgtt ttggaaacag
     5641 tcttcttgaa agtgtcttgt ctacagtata ttcactcata acctcaatag ccaagggtgt
     5701 agtcggttta ttaaaggaag ggagttgtgg ctgatgtgga tagatatctt taaagctggc
     5761 gactgcaccc aacgagtgtg gtggtagctt gttactgtat attcgaattc gtataacttc
     5821 gtatagcagg agttatccga agcgataatt accctgttat ccctaacggg gccctttcgt
     5881 cacgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggtgacggtc
     5941 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     6001 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     6061 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     6121 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     6181 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     6241 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcgagctcgg tacccgcttt
     6301 tcgtagataa tggaatacaa atggatatcc agagtataca catggatagt atacactgac
     6361 acgacaattc tgtatctctt tatgttaact actgtgaggc gttaaataga gcttgatata
     6421 taaaatgtta catttcacag tctgaacttt tgcagattac ctaatttggt aagatattaa
     6481 ttatgaactg aaagttgatg gcatccctaa atttgatgaa agggggaacc cccgggttcg
     6541 aaggtaccaa ggaagcatgc ggtacccgaa ttcgcttcga tacgcgtatc gatgatacgc
     6601 gtccatggtg aggtgtctca caagtgccgt gcagtcccgc ccccacttgc ttctctttgt
     6661 gtgtagtgta cgtacattat cgagagggtt gttcccgccc acctcgatcc ggcatgctga
     6721 ggtgtctcac aagtgccgtg cagtcccgcc cccacttgct tctctttgtg tgtagtgtac
     6781 gtacattatc gagagggttg ttcccgccca cctcgatccg gcatgctgag gtgtctcaca
     6841 agtgccgtgc agtcccgccc ccacttgctt ctctttgtgt gtagtgtacg tacattatcg
     6901 agagggttgt tcccgcccac ctcgatccgg catgctgagg tgtctcacaa gtgccgtgca
     6961 gtcccgcccc cacttgcttc tctttgtgtg tagtgtacgt acattatcga gagggttgtt
     7021 cccgcccacc tcgatccggc atgctgaggt gtctcacaag tgccgtgcag tcccgccccc
     7081 acttgcttct ctttgtgtgt agtgtacgta cattatcgag agggttgttc ccgcccacct
     7141 cgatccggca tgctgaggtg tctcacaagt gccgtgcagt cccgccccca cttgcttctc
     7201 tttgtgtgta gtgtacgtac attatcgaga gggttgttcc cgcccacctc gatccggcat
     7261 gctgaggtgt ctcacaagtg ccgtgcagtc ccgcccccac ttgcttctct ttgtgtgtag
     7321 tgtacgtaca ttatcgagag ggttgttccc gcccacctcg atccgacatg ttgaggtgtc
     7381 tcacaagtgc cgtgcagtcc cgcccccact tgcttctctt tgtgtgtagt gtacgtacat
     7441 tatcgagagg gttgttcccg cccacctcga tccggcatgc actgatcacg ggcaaaagtg
     7501 cttcgataga gagggggttg gcggcgcatt tgtgtcccaa aaaacagccc caattgcccc
     7561 aattgacccc aaattgaccc agtagcggac ccaaccccgg cgagagcccc cttcacccca
     7621 catatcaaac ctcccccggt tcccacactt gccgttaagg gcgtagggta ctgcagtctg
     7681 gaatctacgc ttgttcagac tttgtactag tttctttgtc tggccatccg ggtaacccat
     7741 gccggacgca aaatagacta ctgaaaattt ttttgctttg tggttgggac tttagccaag
     7801 ggtataaaag accaccgtcc ccgaattacc tttcctcttc ttttctctct ctccttgtca
     7861 actcacaccc gaaggatcca caaccacaat ggataagaaa tactccattg gcctggacat
     7921 cggaaccaac tccgtgggtt gggccgtgat caccgatgag tacaaggtgc cctctaagaa
     7981 attcaaggtc ctgggcaaca ccgaccgaca ctccatcaag aagaacctga tcggcgctct
     8041 gctcttcgac tctggcgaga ctgctgaggc cacccgactg aagcgaaccg ctcgaagacg
     8101 atacacccga agaaagaacc gaatctgtta cctgcaggag atcttctcta acgagatggc
     8161 caaggtggac gactctttct tccaccgact ggaggagtct ttcctggtgg aggaggacaa
     8221 gaagcacgag cgacacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa
     8281 gtaccccacc atctaccacc tgcgaaagaa gctggtggac tctaccgaca aggccgacct
     8341 gcgactgatc tacctggccc tggcccacat gatcaagttc cgaggccact tcctgatcga
     8401 gggcgacctg aaccccgaca actctgacgt ggacaagctg ttcatccagc tggtgcagac
     8461 ctacaaccag ctcttcgaag agaaccccat taacgcttct ggcgtggatg ctaaggccat
     8521 cctgtctgcc cgactgtcta agtctcgacg actcgagaac ctgattgctc agctccccgg
     8581 agagaagaag aacggtctgt tcggaaacct gattgctctg tccctgggtc tgacccctaa
     8641 cttcaagtcc aacttcgatc tggctgagga cgctaagctg cagctgtcta aggacaccta
     8701 cgacgatgac ctggataacc tgctcgccca gattggcgac cagtacgccg acctgttcct
     8761 ggccgccaag aacctgtctg acgccatcct gctgtctgac atcctgcgag tgaacaccga
     8821 gatcaccaag gcccccctgt ctgcctccat gattaagcga tacgatgagc accaccagga
     8881 tctgaccctc ctcaaggctc tggtccgaca gcagctgccc gagaagtaca aggagatttt
     8941 cttcgaccag tctaagaacg gctacgccgg ctacatcgac ggcggcgcct ctcaggagga
     9001 gttctacaag ttcattaagc ccatcctgga gaagatggac ggaaccgagg aactgctcgt
     9061 gaagctgaac cgagaggacc tcctgcgaaa gcagcgaacc ttcgacaacg gctctatccc
     9121 ccaccagatc cacctgggcg agctgcacgc catcctgcga cgacaggagg acttctaccc
     9181 cttcctgaag gacaaccgag agaagatcga gaagatcctg accttccgaa tcccctacta
     9241 cgtgggaccc ctggcccgag gaaactctcg attcgcttgg atgacccgaa agtctgagga
     9301 gactattacc ccctggaact tcgaggaggt ggtggataag ggcgcctctg ctcagtcttt
     9361 catcgagcga atgaccaact tcgacaagaa cctccccaac gagaaggtcc tgcccaagca
     9421 ctctctgctc tacgagtact tcaccgtcta caacgagctc accaaggtca agtacgtgac
     9481 cgagggaatg cgaaagcccg ctttcctgtc tggagagcag aagaaggcta ttgtggatct
     9541 gctcttcaag actaaccgaa aggtcaccgt caagcagctg aaggaggatt acttcaagaa
     9601 gattgagtgt ttcgattctg tcgagatctc cggcgtcgag gaccgattca acgcctctct
     9661 gggtacctac cacgacctgc tgaagattat caaggacaag gatttcctgg ataacgagga
     9721 gaacgaggat attctcgagg acattgtcct gaccctcacc ctgttcgagg atcgagagat
     9781 gattgaggag cgactcaaga cctacgctca cctgttcgac gacaaggtga tgaagcagct
     9841 gaagcgacga cgatacaccg gctggggccg actgtctcga aagctgatca acggcatccg
     9901 agacaagcag tctggcaaga ccatcctgga cttcctgaag tctgacggct tcgccaaccg
     9961 aaacttcatg cagctgatcc acgacgactc tctgaccttc aaggaggaca tccagaaggc
    10021 ccaggtgtct ggccagggcg actctctgca cgagcacatc gccaacctgg ccggctctcc
    10081 cgccattaag aaaggtatcc tgcagaccgt caaggtggtc gatgagctcg tcaaggtgat
    10141 gggccgacac aagcccgaga acattgtcat tgagatggct cgagagaacc agactactca
    10201 gaagggtcag aaaaactccc gagagcgaat gaagcgaatt gaggaaggta ttaaggagct
    10261 gggctcccag attctcaagg agcatcccgt ggagaacact cagctccaga acgagaagct
    10321 gtacctgtac tatctgcaga acggtcgaga catgtacgtc gaccaggagc tggatatcaa
    10381 ccgactctcc gactacgatg tggaccacat tgtgccccag tccttcctga aggacgattc
    10441 tatcgataac aaggtgctga cccgatccga caagaaccga ggcaagtctg acaacgtgcc
    10501 ctccgaggag gtggtcaaga agatgaagaa ctactggcga cagctgctga acgccaagct
    10561 gattacccag cgaaagttcg acaacctgac caaggccgag cgaggcggcc tgtctgagct
    10621 ggacaaggcc ggcttcatca agcgacagct ggtggagact cgacagatca ccaagcacgt
    10681 ggcccagatc ctggactctc gaatgaacac caagtacgac gagaacgaca agctgatccg
    10741 agaggtgaag gtgatcaccc tgaagtctaa gctggtgtct gacttccgaa aggacttcca
    10801 gttctacaag gtgcgagaga ttaacaacta ccaccacgcc cacgatgcct acctgaacgc
    10861 tgtcgtgggc accgccctca tcaagaagta tcccaagctg gagtccgagt tcgtctacgg
    10921 cgactacaag gtctacgatg tgcgaaaaat gattgccaag tccgagcagg agattggcaa
    10981 ggctaccgcc aagtacttct tctactccaa cattatgaac ttcttcaaga ccgagattac
    11041 cctggctaac ggcgagattc gaaagcgacc cctcattgag actaacggag agactggtga
    11101 gatcgtgtgg gacaagggac gagacttcgc caccgtgcga aaggtgctgt ctatgcccca
    11161 ggtgaacatc gtgaagaaga ccgaggtgca gaccggaggt ttctctaagg agtccatcct
    11221 gcccaagcga aactctgaca agctgatcgc ccgaaagaag gactgggacc ccaagaagta
    11281 cggaggtttc gactctccca ccgtggctta ctctgtgctg gtggtggcca aggtggagaa
    11341 gggcaagtct aagaagctga agtctgtgaa ggagctgctg ggcattacca tcatggagcg
    11401 atcttctttc gagaagaacc ccattgactt cctggaggcc aagggataca aggaggtgaa
    11461 gaaagatctg attatcaagc tccccaagta ctctctgttc gagctggaga acggacgaaa
    11521 gcgaatgctg gcctctgccg gcgagctgca gaagggaaac gagctggccc tgccctccaa
    11581 gtacgtcaac ttcctgtacc tcgcctccca ttacgagaag ctgaagggct ctcccgagga
    11641 taacgagcag aagcagctct tcgtggagca gcataagcac tacctggacg agatcatcga
    11701 gcagatttct gagttctcta agcgagtgat cctggctgac gccaacctgg ataaggtgct
    11761 gtctgcttac aacaagcacc gagacaagcc cattcgagag caggctgaga acatcattca
    11821 cctgttcacc ctgaccaacc tgggagcccc cgctgccttc aagtacttcg acaccaccat
    11881 cgaccgaaag cgatacacct ctaccaagga ggtgctggac gccaccctga tccaccagtc
    11941 tatcaccggc ctgtacgaga ctcgaatcga cctgtctcag ctgggcggcg actctcgagc
    12001 cgaccccaag aagaagcgaa aggtgtaagc tagcctcatg taattagtta tgtcacgctt
    12061 acattcacgc cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga
    12121 agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt
    12181 tcaaattttt cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac
    12241 cttgcttgag aaggttttgg gacgctcgaa ggctttaatt tgcaagcttg gcggtct
//