| 质粒名称: | pAAVLINK-3'-SPTBN1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001714 |
| 产品用途: | 3' AAVLINK plasmid for SPTBN1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SPTBN1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-3'-SPTBN1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-3'-SPTBN1-FLAG载体质粒基本信息
pAAVLINK-3'-SPTBN1-FLAG质粒图谱载体图谱和pAAVLINK-3'-SPTBN1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINK-3'-SPTBN1-FLAG质粒载体简介
pAAVLINK-3'-SPTBN1-FLAG质粒序列载体序列
LOCUS pAAVLINK-3'-SPTBN1-FLAG 7233 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7233)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 3, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7233
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
protein_bind 145..178
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..3972
/codon_start=1
/label=SPTBN1
/translation="GQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFL
RDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRR
LVSDGNINSDRIQEKVDSIDDRHRKNRETASELLMRLKDNRDLQKFLQDCQELSLWINE
KMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVK
EKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKD
LTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMEL
LEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQT
LQKEIQGHQPRIDDIFERSQNIVTDSSSLSAEAIRQRLADLKQLWGLLIEETEKRHRRL
EEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAET
VHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNRE
VDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHLADEL
INSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDK
HKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQ
KRENEVLEAWKSLLDACESRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRD
VSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKE
MIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHE
AFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKVSEEAESQQQW
DTSKGEQVSQNGLPAEQGSPRMAETVDTSEMVNGATEQRTSSKESSPIPSPTSDRKAKT
ALPAQSAATLPARTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFY
KDAKTAASGIPYHSEVPVSLKEAVCEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMN
TWIQAISSAISSDKHEVSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEK
RFSLFGKKK"
misc_feature 1646..1651
/label=SDS
misc_feature 2247..2252
/label=SDS
misc_feature 2471..2476
/label=SDS
misc_feature 2565..2570
/label=SDS
CDS 3979..4044
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4048..4479
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4192..4197
/label=SDS
repeat_region 4496..4636
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4496..4625
/label=AAV2 ITR
rep_origin 4711..5166
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5448..5552
/gene="bla"
/label=AmpR promoter
CDS 5553..6413
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6584..7172
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag gggcagaccg atgcccagta catgtttctg
301 cggcagcggc tgcaggccct ggacactgga tggaacgagc tccacaagat gtgggagaac
361 agacaaaatc tcctatccca gtcacatgcc taccagcagt tcctcagaga cacgaagcaa
421 gccgaagcct ttcttaacaa ccaggagtat gttctggctc acactgaaat gcctaccacc
481 ttggaaggag ctgaagcagc aattaaaaag caagaggact tcatgaccac catggacgcc
541 aatgaggaga agatcaatgc tgtggtggag actggccgga ggctggtgag cgatgggaac
601 atcaactcag atcgcatcca ggagaaggtg gactctattg atgacagaca taggaagaat
661 cgtgagacag ccagtgaact tttgatgagg ttgaaggaca acagggatct acagaaattc
721 ctgcaagatt gtcaagagct gtctctctgg atcaatgaga agatgctcac agcccaggac
781 atgtcttacg atgaagccag aaatctgcac agtaaatggt tgaagcatca agcatttatg
841 gcagaacttg catccaacaa agaatggctt gacaaaatcg agaaggaagg aatgcagctc
901 atttcagaaa agcctgagac ggaagctgtg gtgaaggaga aactcactgg tttacataaa
961 atgtgggaag tccttgaatc cactacccag acaaaggccc agcggctctt tgatgcaaac
1021 aaggccgaac ttttcaccca gagctgtgca gatctagaca aatggctgca cggcctggag
1081 agtcagattc agtctgatga ctatggcaaa gacctgacca gtgtcaatat cctgctgaaa
1141 aagcaacaga tgctggagaa tcagatggaa gtgcggaaga aggagatcga agagctccaa
1201 agccaagccc aggccctgag tcaggaaggg aagagcaccg acgaggtaga cagcaagcgc
1261 ctcaccgtgc agaccaagtt catggagttg ctggagccct tgaacgagag gaagcataac
1321 ctgctggcct ccaaagagat ccatcagttc aacagggatg tggaggacga gatcttgtgg
1381 gttggagaga ggatgccttt ggcaacttcc acggatcatg gccacaacct ccagactgtg
1441 cagctgttaa taaagaaaaa tcagaccctc cagaaagaaa tccaggggca ccagcctcgc
1501 attgacgaca tctttgagag gagccaaaac atcgtcactg acagcagcag cctcagcgct
1561 gaggccatca gacagaggct tgccgacctg aagcagctgt ggggtctcct cattgaggag
1621 acagagaaac gccacaggcg gctggaggag gcgcacaggg cccagcagta ctactttgac
1681 gctgctgagg ccgaagcctg gatgagcgag caggagctgt acatgatgtc agaggagaag
1741 gccaaggatg agcagagtgc tgtctccatg ttgaagaagc accagatctt agaacaagct
1801 gtggaggact atgcagagac cgtgcatcag ctctccaaga ccagccgggc cctggtggcc
1861 gacagccatc ctgaaagtga gcgcattagc atgcggcagt ccaaagtgga taaactgtac
1921 gctggtctga aagaccttgc tgaagagaga agaggcaagc tggatgagag acacaggtta
1981 ttccagctca accgggaggt ggacgacctg gagcagtgga tcgctgagag ggaggtggtc
2041 gcagggtccc atgaactggg acaggactat gagcatgtca cgatgttaca agaacgattc
2101 cgggagtttg cccgagacac cgggaacatt gggcaggagc gcgtggacac ggtcaatcac
2161 ctggcagatg agctcatcaa ctctggacat tcagatgccg ccaccatcgc tgaatggaag
2221 gatggcctca atgaagcctg ggccgacctc ctggagctca ttgacacaag aacacagatt
2281 cttgccgctt cctatgaact gcacaagttt taccacgatg ccaaggagat ctttgggcgt
2341 atacaggaca aacacaagaa actccctgag gagcttggga gagatcagaa cacagtggag
2401 accttacaga gaatgcacac tacatttgag catgacatcc aggctctggg cacacaggtg
2461 aggcagctgc aggaggatgc agcccgcctc caggcggcct atgcgggtga caaggccgac
2521 gatatccaga agcgcgagaa cgaggtcctg gaagcctgga agtccctcct ggacgcctgt
2581 gagagccgca gggtgcggct ggtggacaca ggggacaagt tccgcttctt cagcatggtg
2641 cgcgacctca tgctctggat ggaggatgtc atccggcaga tcgaggccca ggagaagcca
2701 agggatgtat catctgttga actcttaatg aataatcatc aaggcatcaa agctgaaatt
2761 gatgcacgta atgacagttt cacaacctgc attgaacttg ggaaatccct gttggcgaga
2821 aaacactatg catctgagga gatcaaggaa aaattactgc agttgacgga aaagaggaaa
2881 gaaatgatcg acaagtggga agaccgatgg gaatggttaa gactgattct ggaggtccat
2941 cagttctcaa gagacgccag tgtggccgag gcctggctgc ttggacagga gccgtaccta
3001 tccagccgag agataggcca gagcgtggac gaggtggaga agctcatcaa gcgccacgag
3061 gcatttgaaa agtctgcagc aacctgggat gagaggttct ctgccctgga aaggctgact
3121 acattggagt tactggaagt gcgcagacag caagaggaag aggagaggaa gaggcggcca
3181 ccttctcccg agccgagcac gaaggtttca gaggaagccg agtcccagca gcagtgggat
3241 acttcaaaag gagaacaagt ttcccaaaac ggtttgccag ctgaacaggg atctccacgg
3301 atggcagaaa cggtggacac aagcgaaatg gtcaacggcg ctacagaaca aaggacgagc
3361 tctaaagagt ccagccccat cccctccccg acctctgatc gtaaagccaa gactgccctc
3421 ccagcccaga gtgccgccac cttaccagcc agaacccagg agacaccttc ggcccagatg
3481 gaaggcttcc tcaatcggaa acacgagtgg gaggcccaca ataagaaagc ctcaagcagg
3541 tcctggcaca atgtttattg tgtcataaat aaccaagaaa tgggtttcta caaagatgca
3601 aagactgctg cttctggaat tccctaccac agcgaggtcc ctgtgagttt gaaagaagct
3661 gtctgcgaag tggcccttga ttacaaaaag aagaaacacg tattcaagct aagactaaat
3721 gatggcaatg agtacctctt ccaagccaaa gacgatgagg aaatgaacac atggatccag
3781 gctatctctt ccgccatctc ctctgataaa cacgaggtgt ctgccagcac ccagagcacg
3841 ccagcatcca gccgcgcgca gaccctcccc accagcgtcg tcaccatcac cagcgagtcc
3901 agtcccggca agcgggaaaa ggacaaagag aaagacaaag agaagcggtt cagccttttt
3961 ggcaaaaaga aatgtacaga ctacaaagac catgacggtg attataaaga tcatgacatc
4021 gactacaagg atgacgatga caagtaactc gagataatca acctctggat tacaaaattt
4081 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
4141 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
4201 ataaatcctg gttagttctt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
4261 ggacaggggc tcggctgttg ggcactgaca attccgtggt gtttatttgt gaaatttgtg
4321 atgctattgc tttatttgta accatctagc tttatttgtg aaatttgtga tgctattgct
4381 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
4441 atgtttcagg ttcaggggga gatgtgggag gttttttaac ggaccgagcg gccgcaggaa
4501 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
4561 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
4621 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
4681 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
4741 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
4801 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
4861 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
4921 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
4981 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
5041 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
5101 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
5161 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
5221 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
5281 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
5341 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
5401 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
5461 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
5521 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
5581 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
5641 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
5701 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
5761 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
5821 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
5881 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
5941 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
6001 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
6061 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
6121 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
6181 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
6241 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
6301 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
6361 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
6421 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
6481 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
6541 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
6601 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
6661 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
6721 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
6781 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
6841 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
6901 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
6961 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
7021 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
7081 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
7141 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
7201 tcctggcctt ttgctggcct tttgctcaca tgt
//
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