pAAVLINK-EF1alpha-HA-5'-SCN2A

LOCUS      pAAVLINK-EF1alpha-HA-5'-SCN2A              7179 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        158..700
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..4319
                     /codon_start=1
                     /gene="SCN2A"
                     /gene_synonym="BFIC3; BFIS3; BFNIS; DEE11; EA9; EIEE11;HBA;
                     HBSCI; HBSCII; Na(v)1.2; NAC2; Nav1.2; SCN2A1; SCN2A2"
                     /product="sodium channel protein type 2 subunit 
                     alphaisoform 1"
                     /label=SCN2A
                     /note="isoform 1 is encoded by transcript variant 2;sodium 
                     channel, voltage-gated, type II, alpha 2 polypeptide; 
                     sodium channel, voltage-gated, type II, alpha 1 
                     polypeptide; sodium channel protein, brain type 2 alpha 
                     subunit; voltage-gated sodium channel subtype II; sodium 
                     channel protein type II subunit alpha; sodium channel 
                     protein brain II subunit alpha; voltage-gated sodium 
                     channel subunit alpha Nav1.2; sodium channel, 
                     voltage-gated, type II, alpha subunit; voltage-gated sodium
                     channel type II alpha subunit"
                     /db_xref="GI:93141212"
                     /db_xref="CCDS:CCDS33314.1"
                     /db_xref="GeneID:6326"
                     /db_xref="HGNC:HGNC:10588"
                     /db_xref="MIM:182390"
                     /protein_id="NP_001035232.1"
                     /translation="MAQSVLVPPGPDSFRFFTRESLAAIEQRIAEEKAKRPKQERKDED
                     DENGPKPNSDLEAGKSLPFIYGDIPPEMVSVPLEDLDPYYINKKTFIVLNKGKAISRFS
                     ATPALYILTPFNPIRKLAIKILVHSLFNMLIMCTILTNCVFMTMSNPPDWTKNVEYTFT
                     GIYTFESLIKILARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVL
                     RALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQW
                     PPDNSSFEINITSFFNNSLDGNGTTFNRTVSIFNWDEYIEDKSHFYFLEGQNDALLCGN
                     SSDAGQCPEGYICVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGK
                     TYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQE
                     EAQAAAAAASAESRDFSGAGGIGVFSESSSVASKLSSKSEKELKNRRKKKKQKEQSGEE
                     EKNDRVRKSESEDSIRRKGFRFSLEGSRLTYEKRFSSPHQSLLSIRGSLFSPRRNSRAS
                     LFSFRGRAKDIGSENDFADDEHSTFEDNDSRRDSLFVPHRHGERRHSNVSQASRASRVL
                     PILPMNGKMHSAVDCNGVVSLVGGPSTLTSAGQLLPEGTTTETEIRKRRSSSYHVSMDL
                     LEDPTSRQRAMSIASILTNTMEELEESRQKCPPCWYKFANMCLIWDCCKPWLKVKHLVN
                     LVVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLSVGNLVFTGIFTAEMFLKIIA
                     MDPYYYFQEGWNIFDGFIVSLSLMELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLI
                     KIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKISNDCELPRWHMHDFFH
                     SFLIVFRVLCGEWIETMWDCMEVAGQTMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSD
                     NLAATDDDNEMNNLQIAVGRMQKGIDFVKRKIREFIQKAFVRKQKALDEIKPLEDLNNK
                     KDSCISNHTTIEIGKDLNYLKDGNGTTSGIGSSVEKYVVDESDYMSFINNPSLTVTVPI
                     AVGESDFENLNTEEFSSESDMEESKEKLNATSSSEGSTVDIGAPAEGEQPEVEPEESLE
                     PEACFTEDCVRKFKCCQISIEEGK"
     intron          4320..4360
                     /label=Splicing Donor
     misc_feature    4364..4397
                     /label=lox2272-JT1571
     repeat_region   4442..4582
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4442..4571
                     /label=AAV2 ITR
     repeat_region   4443..4583
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4657..5112
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5394..5498
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5499..6359
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6530..7118
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctat ggcacagtca gtgctggtac cgccaggacc
      781 tgacagcttc cgcttcttta ccagggaatc ccttgctgct attgaacaac gcattgcaga
      841 agagaaagct aagagaccca aacaggaacg caaggatgag gatgatgaaa atggcccaaa
      901 gccaaacagt gacttggaag caggaaaatc tcttccattt atttatggag acattcctcc
      961 agagatggtg tcagtgcccc tggaggatct ggacccctac tatatcaata agaaaacgtt
     1021 tatagtattg aataaaggga aagcaatctc tcgattcagt gccacccctg ccctttacat
     1081 tttaactccc ttcaacccta ttagaaaatt agctattaag attttggtac attctttatt
     1141 caatatgctc attatgtgca cgattcttac caactgtgta tttatgacca tgagtaaccc
     1201 tccagactgg acaaagaatg tggagtatac ctttacagga atttatactt ttgaatcact
     1261 tattaaaata cttgcaaggg gcttttgttt agaagatttc acatttttac gggatccatg
     1321 gaattggttg gatttcacag tcattacttt tgcatatgtg acagagtttg tggacctggg
     1381 caatgtctca gcgttgagaa cattcagagt tctccgagca ttgaaaacaa tttcagtcat
     1441 tccaggcctg aagaccattg tgggggccct gatccagtca gtgaagaagc tttctgatgt
     1501 catgatcttg actgtgttct gtctaagcgt gtttgcgcta ataggattgc agttgttcat
     1561 gggcaaccta cgaaataaat gtttgcaatg gcctccagat aattcttcct ttgaaataaa
     1621 tatcacttcc ttctttaaca attcattgga tgggaatggt actactttca ataggacagt
     1681 gagcatattt aactgggatg aatatattga ggataaaagt cacttttatt ttttagaggg
     1741 gcaaaatgat gctctgcttt gtggcaacag ctcagatgca ggccagtgtc ctgaaggata
     1801 catctgtgtg aaggctggta gaaaccccaa ctatggctac acgagctttg acacctttag
     1861 ttgggccttt ttgtccttat ttcgtctcat gactcaagac ttctgggaaa acctttatca
     1921 actgacacta cgtgctgctg ggaaaacgta catgatattt tttgtgctgg tcattttctt
     1981 gggctcattc tatctaataa atttgatctt ggctgtggtg gccatggcct atgaggaaca
     2041 gaatcaggcc acattggaag aggctgaaca gaaggaagct gaatttcagc agatgctcga
     2101 acagttgaaa aagcaacaag aagaagctca ggcggcagct gcagccgcat ctgctgaatc
     2161 aagagacttc agtggtgctg gtgggatagg agttttttca gagagttctt cagtagcatc
     2221 taagttgagc tccaaaagtg aaaaagagct gaaaaacaga agaaagaaaa agaaacagaa
     2281 agaacagtct ggagaagaag agaaaaatga cagagtccga aaatcggaat ctgaagacag
     2341 cataagaaga aaaggtttcc gtttttcctt ggaaggaagt aggctgacat atgaaaagag
     2401 attttcttct ccacaccagt ccttactgag catccgtggc tcccttttct ctccaagacg
     2461 caacagtagg gcgagccttt tcagcttcag aggtcgagca aaggacattg gctctgagaa
     2521 tgactttgct gatgatgagc acagcacctt tgaggacaat gacagccgaa gagactctct
     2581 gttcgtgccg cacagacatg gagaacggcg ccacagcaat gtcagccagg ccagccgtgc
     2641 ctccagggtg ctccccatcc tgcccatgaa tgggaagatg catagcgctg tggactgcaa
     2701 tggtgtggtc tccctggtcg ggggcccttc taccctcaca tctgctgggc agctcctacc
     2761 agagggcaca actactgaaa cagaaataag aaagagacgg tccagttctt atcatgtttc
     2821 catggattta ttggaagatc ctacatcaag gcaaagagca atgagtatag ccagtatttt
     2881 gaccaacacc atggaagaac ttgaagaatc cagacagaaa tgcccaccat gctggtataa
     2941 atttgctaat atgtgtttga tttgggactg ttgtaaacca tggttaaagg tgaaacacct
     3001 tgtcaacctg gttgtaatgg acccatttgt tgacctggcc atcaccatct gcattgtctt
     3061 aaatacactc ttcatggcta tggagcacta tcccatgacg gagcagttca gcagtgtact
     3121 gtctgttgga aacctggtct tcacagggat cttcacagca gaaatgtttc tcaagataat
     3181 tgccatggat ccatattatt actttcaaga aggctggaat atttttgatg gttttattgt
     3241 gagccttagt ttaatggaac ttggtttggc aaatgtggaa ggattgtcag ttctccgatc
     3301 attccggctg ctccgagttt tcaagttggc aaaatcttgg ccaactctaa atatgctaat
     3361 taagatcatt ggcaattctg tgggggctct aggaaacctc accttggtat tggccatcat
     3421 cgtcttcatt tttgctgtgg tcggcatgca gctctttggt aagagctaca aagaatgtgt
     3481 ctgcaagatt tccaatgatt gtgaactccc acgctggcac atgcatgact ttttccactc
     3541 cttcctgatc gtgttccgcg tgctgtgtgg agagtggata gagaccatgt gggactgtat
     3601 ggaggtcgct ggccaaacca tgtgccttac tgtcttcatg atggtcatgg tgattggaaa
     3661 tctagtggtt ctgaacctct tcttggcctt gcttttgagt tccttcagtt ctgacaatct
     3721 tgctgccact gatgatgata acgaaatgaa taatctccag attgctgtgg gaaggatgca
     3781 gaaaggaatc gattttgtta aaagaaaaat acgtgaattt attcagaaag cctttgttag
     3841 gaagcagaaa gctttagatg aaattaaacc gcttgaagat ctaaataata aaaaagacag
     3901 ctgtatttcc aaccatacca ccatagaaat aggcaaagac ctcaattatc tcaaagacgg
     3961 aaatggaact actagtggca taggcagcag tgtagaaaaa tatgtcgtgg atgaaagtga
     4021 ttacatgtca tttataaaca accctagcct cactgtgaca gtaccaattg ctgttggaga
     4081 atctgacttt gaaaatttaa atactgaaga attcagcagc gagtcagata tggaggaaag
     4141 caaagagaag ctaaatgcaa ctagttcatc tgaaggcagc acggttgata ttggagctcc
     4201 cgccgaggga gaacagcctg aggttgaacc tgaggaatcc cttgaacctg aagcctgttt
     4261 tacagaagac tgtgtacgga agttcaagtg ttgtcagata agcatagaag aaggcaaagg
     4321 taagtatcaa ggttacaaga caggtttaag gagaccaata agctattatt cgtataggat
     4381 actttatacg aagttattaa gttatggaga ccggtgtggc acgtgcggac cgagcggccg
     4441 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     4501 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     4561 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     4621 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     4681 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
     4741 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4801 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4861 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4921 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4981 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
     5041 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5101 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5161 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5221 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5281 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5341 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5401 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5461 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5521 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5581 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5641 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5701 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5761 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5821 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5881 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5941 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6001 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6061 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6121 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6181 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6241 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6301 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6361 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6421 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6481 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6541 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6601 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6661 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6721 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6781 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6841 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6901 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6961 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7021 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7081 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7141 tacggttcct ggccttttgc tggccttttg ctcacatgt
//