| 出品公司: | Origene |
|---|---|
| 载体名称: | pCas-Scramble |
| 质粒类型: | 哺乳动物细胞载体；crispr/Cas9基因敲除载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 8.0 kb |
| 5’测序引物: | -- |
| 3’测序引物: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5 alpha; 等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 产品目录号: | GE100003 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 备注: |
买家导航
pCas-Scramble载体质粒基本信息
pCas-Scramble质粒图谱载体图谱和pCas-Scramble载体序列质粒序列多克隆位点信息
pCas-Scramble质粒载体简介
pCas-Scramble质粒序列载体序列
LOCUS pCas-Scramble 7997 bp DNA circular SYN 03-NOV-2024
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7997)
AUTHORS Ming
TITLE Direct Submission
JOURNAL Exported Nov 3, 2024 from SnapGene 6.0.2 to Vector NTI(R) format
https://www.biofeng.com
COMMENT VNTDATE|1073001600|
COMMENT VNTDBDATE|1073001600|
COMMENT VNTNAME|pCas-Scramble|
COMMENT VNTAUTHORNAME|Ming|
FEATURES Location/Qualifiers
promoter 90..330
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
misc_RNA 359..434
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
enhancer 486..865
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 866..1069
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 1095..1113
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1172..5275
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
CDS 5276..5296
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
CDS 5303..5323
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
CDS 5345..5374
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
CDS 5393..5416
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
primer_bind complement(5436..5452)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
polyA_signal 5463..6085
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
rep_origin complement(6206..6794)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6958..7818)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
primer_bind 7933..7949
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 7954..7972
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 gaattcccca gtggaaagac gcgcaggcaa aacgcaccac gtgacggagc gtgaccgcgc
61 gccgagcgcg cgccaaggtc gggcaggaag agggcctatt tcccatgatt ccttcatatt
121 tgcatatacg atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa
181 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
241 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
301 tcttgggttt atatatcttg tggaaaggac gcgggatcgc actaccagag ctaactcagt
361 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
421 caccgagtcg gtgctttttt tggtgtacat ttatattggc tcatgtccaa tatgaccgcc
481 atgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
541 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
601 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
661 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
721 acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc
781 cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta
841 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca atgggcgtgg
901 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
961 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg ccccgttgac
1021 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
1081 ccgtcagaat tttgtaatac gactcactat agggcggccg ggaattcgtc gactggaacc
1141 ggtaccgagg agatctgccg ccgcgatcgc catggataag aaatactcaa taggactgga
1201 tattggcaca aatagcgtcg gatgggctgt gatcactgat gaatataagg ttccttctaa
1261 aaagttcaag gttctgggaa atacagaccg ccacagtatc aaaaaaaatc ttataggggc
1321 tcttctgttt gacagtggag agacagccga agctactaga ctcaaacgga cagctaggag
1381 aaggtataca agacggaaga ataggatttg ttatctccag gagatttttt caaatgagat
1441 ggccaaagtg gatgatagtt tctttcatag acttgaagag tcttttttgg tggaagaaga
1501 caagaagcat gaaagacatc ctatttttgg aaatatagtg gatgaagttg cttatcacga
1561 gaaatatcca actatctatc atctgagaaa aaaattggtg gattctactg ataaagccga
1621 tttgcgcctg atctatttgg ccctggccca catgattaag tttagaggtc attttttgat
1681 tgagggcgat ctgaatcctg ataatagtga tgtggacaaa ctgtttatcc agttggtgca
1741 aacctacaat caactgtttg aagaaaaccc tattaacgca agtggagtgg atgctaaagc
1801 cattctttct gcaagattga gtaaatcaag aagactggaa aatctcattg ctcagctccc
1861 cggtgagaag aaaaatggcc tgtttgggaa tctcattgct ttgtcattgg gtttgacccc
1921 taattttaaa tcaaattttg atttggcaga agatgctaaa ctccagcttt caaaagatac
1981 ttacgatgat gatctggata atctgttggc tcaaattgga gatcaatatg ctgatttgtt
2041 tttggcagct aagaatctgt cagatgctat tctgctttca gacatcctga gagtgaatac
2101 tgaaataact aaggctcccc tgtcagcttc aatgattaaa cgctacgatg aacatcatca
2161 agacttgact cttctgaaag ccctggttag acaacaactt ccagaaaagt ataaagaaat
2221 cttttttgat caatcaaaaa acggatatgc aggttatatt gatggcggcg caagccaaga
2281 agaattttat aaatttatca aaccaattct ggaaaaaatg gatggtactg aggaactgtt
2341 ggtgaaactg aatagagaag atttgctgcg caagcaacgg acctttgaca acggctctat
2401 tccccatcaa attcacttgg gtgagctgca tgctattttg agaagacaag aagactttta
2461 tccatttctg aaagacaata gagagaagat tgaaaaaatc ttgactttta ggattcctta
2521 ttatgttggt ccattggcca gaggcaatag taggtttgca tggatgactc ggaagtctga
2581 agaaacaatt accccatgga attttgaaga agttgtcgat aaaggtgctt cagctcaatc
2641 atttattgaa cgcatgacaa actttgataa aaatcttcca aatgaaaaag tgctgccaaa
2701 acatagtttg ctttatgagt attttaccgt ttataacgaa ttgacaaagg tcaaatatgt
2761 tactgaagga atgagaaaac cagcatttct ttcaggtgaa cagaagaaag ccattgttga
2821 tctgctcttc aaaacaaata ggaaagtgac cgttaagcaa ctgaaagaag attatttcaa
2881 aaaaatagaa tgttttgata gtgttgaaat ttcaggagtt gaagatagat ttaatgcttc
2941 actgggtaca taccatgatt tgctgaaaat tattaaagat aaagattttt tggataatga
3001 agaaaatgaa gacatcctgg aggatattgt tctgacattg accctgtttg aagataggga
3061 gatgattgag gaaagactta aaacatacgc tcacctcttt gatgataagg tgatgaaaca
3121 gcttaaaaga cgcagatata ctggttgggg aaggttgtcc agaaaattga ttaatggtat
3181 tagggataag caatctggca aaacaatact ggattttttg aaatcagatg gttttgccaa
3241 tcgcaatttt atgcagctca tccatgatga tagtttgaca tttaaagaag acatccaaaa
3301 agcacaagtg tctggacaag gcgatagtct gcatgaacat attgcaaatc tggctggtag
3361 ccctgctatt aaaaaaggta ttctccagac tgtgaaagtt gttgatgaat tggtcaaagt
3421 gatggggcgg cataagccag aaaatatcgt tattgaaatg gcaagagaaa atcagacaac
3481 tcaaaagggc cagaaaaatt ccagagagag gatgaaaaga atcgaagaag gtatcaaaga
3541 actgggaagt cagattctta aagagcatcc tgttgaaaat actcaattgc aaaatgaaaa
3601 gctctatctc tattatctcc aaaatggaag agatatgtat gtggaccaag aactggatat
3661 taataggctg agtgattatg atgtcgatca cattgttcca caaagtttcc ttaaagacga
3721 ttcaatagac aataaggtcc tgaccaggtc tgataaaaat agaggtaaat ccgataacgt
3781 tccaagtgaa gaagtggtca aaaagatgaa aaactattgg agacaacttc tgaacgccaa
3841 gctgatcact caaaggaagt ttgataatct gaccaaagct gaaagaggag gtttgagtga
3901 acttgataaa gctggtttta tcaaacgcca attggttgaa actcgccaaa tcactaagca
3961 tgtggcacaa attttggata gtcgcatgaa tactaaatac gatgaaaatg ataaacttat
4021 tagagaggtt aaagtgatta ccctgaaatc taaactggtt tctgacttca gaaaagattt
4081 ccaattctat aaagtgagag agattaacaa ttaccatcat gcccatgatg cctatctgaa
4141 tgccgtcgtt ggaactgctt tgattaagaa atatccaaaa cttgaaagcg agtttgtcta
4201 tggtgattat aaagtttatg atgttaggaa aatgattgct aagtctgagc aagaaatagg
4261 caaagcaacc gcaaagtatt tcttttactc taatatcatg aacttcttca aaacagaaat
4321 tacacttgca aatggagaga ttcgcaaacg ccctctgatc gaaactaatg gggaaactgg
4381 agaaattgtc tgggataaag ggagagattt tgccacagtg cgcaaagtgt tgtccatgcc
4441 ccaagtcaat atcgtcaaga aaacagaagt gcagacaggc ggattctcta aggagtcaat
4501 tctgccaaaa agaaattccg acaagctgat tgctaggaaa aaagactggg acccaaaaaa
4561 atatggtggt tttgatagtc caaccgtggc ttattcagtc ctggtggttg ctaaggtgga
4621 aaaagggaaa tccaagaagc tgaaatccgt taaagagctg ctggggatca caattatgga
4681 aagaagttcc tttgaaaaaa atcccattga ctttctggaa gctaaaggat ataaggaagt
4741 taaaaaagac ctgatcatta aactgcctaa atatagtctt tttgagctgg aaaacggtag
4801 gaaacggatg ctggctagtg ccggagaact gcaaaaagga aatgagctgg ctctgccaag
4861 caaatatgtg aattttctgt atctggctag tcattatgaa aagttgaagg gtagtccaga
4921 agataacgaa caaaaacaat tgtttgtgga gcagcataag cattatctgg atgagattat
4981 tgagcaaatc agtgaatttt ctaagagagt tattctggca gatgccaatc tggataaagt
5041 tcttagtgca tataacaaac atagagacaa accaataaga gaacaagcag aaaatatcat
5101 tcatctgttt accttgacca atcttggagc acccgctgct tttaaatact ttgatacaac
5161 aattgatagg aaaagatata cctctacaaa agaagttctg gatgccactc ttatccatca
5221 atccatcact ggtctttatg aaacacgcat tgatttgagt cagctgggag gtgaccccaa
5281 gaaaaaacgc aaggtggaag atcctaagaa aaagcggaaa gtggacacgc gtacgcggcc
5341 gctcgagcag aaactcatct cagaagagga tctggcagca aatgatatcc tggattacaa
5401 ggatgacgac gataaggttt aaacggccgg ccgcggtcat agctgtttcc tgaacagatc
5461 ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc
5521 cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt
5581 ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga
5641 caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt
5701 ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt
5761 tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagaggc
5821 ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg atctacccac
5881 cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc
5941 tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg ctacctggcc
6001 atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca tgcgttgggt
6061 ccactcagta gatgcctgtt gaattgggta cgcggccagc ggcgagcggt atcagctcac
6121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtcc
6181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
6241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
6301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
6361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
6421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
6481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
6541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
6601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
6661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
6721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
6781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
6841 acgcgtaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
6901 atccttttgc ggccgcaaat caatctaaag tatatatgag taaacttggt ctgacagtta
6961 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
7021 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
7081 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
7141 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
7201 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
7261 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
7321 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
7381 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
7441 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
7501 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
7561 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
7621 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
7681 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
7741 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
7801 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
7861 ttgtctcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacacg
7921 ggccagagct gccaggaaac agctatgacc atgtaatacg actcactata ggggatatca
7981 gctggatggc agttaac
//
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