pVgRxR

LOCUS       pVgRXR                  8854 bp ds-DNA     circular SYN 11-NOV-2022
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 11, 2022 from SnapGene Viewer 5.1.4.1 to Vector
            NTI(R) format
            https://www.snapgene.com
COMMENT     VNTDATE|1001808000|
COMMENT     VNTDBDATE|1001808000|
COMMENT     VNTNAME|pVgRXR|
FEATURES             Location/Qualifiers
     promoter        81..410
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      261..396
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        840..887
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        879..897
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      901..910
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             907..1281
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
     polyA_signal    1345..1479
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1643..2231)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        2316..2619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2620..2823
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        2958..2976
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3080..3089
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3089..3322
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
     polyA_signal    5586..5634
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        6403..6664
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     promoter        complement(8115..8133)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    8159..8383
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8429..8809
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ncbcdnarvg rrvgrrbcnv tvgrrgvgrr rdrybcntch ybcncyrght cattatggtg
       61 accggatccg gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
      121 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
      181 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
      241 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
      301 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
      361 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctggctc
      421 gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggtgagtcg
      481 cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaggctc
      541 caagggagcg ccggacaaag gcccggtctc gacctgagct ctaaacttac ctagactcag
      601 ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc
      661 tgttctgcgc cgttacaact tcaaggtatg cgctgggacc tggcaggcgg catctgggac
      721 ccctaggaag ggcttggggg tcctcgtgcc caaggcaggg aacatagtgg tcccaggaag
      781 gggagcagag gcatcagggt gtccactttg tctccgcagc tcctgagcct gcagcacgtg
      841 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgactca ctataggagg
      901 gccaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
      961 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
     1021 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
     1081 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
     1141 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
     1201 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
     1261 gtggccgagg agcaggactg accgacgccg accaacaccg ccggtccgac gcggcccgac
     1321 gggtccgagg ggggtcgacc tcgaaacttg tttattgcag cttataatgg ttacaaataa
     1381 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     1441 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc ctcggagatc gatctgggcc
     1501 catgcggccg gccgcggatc gatgctcact caaaggcggt aatacggtta tccacagaat
     1561 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1621 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1681 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1741 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     1801 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     1861 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1921 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1981 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2041 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     2101 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2161 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2221 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2281 actcacgtta agggattttg gtcatggcta gagtccgtta cataacttac ggtaaatggc
     2341 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2401 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     2461 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2521 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2581 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2641 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     2701 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     2761 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     2821 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     2881 agaagacacc gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacggacctg
     2941 cagcacgtgt tgacaattaa tacgactcac tatagggaga cccaagcttg gtaccagatc
     3001 ttttaaagct agctctagag taccgagctc ggatcccccg ggccagtgtg atggatatct
     3061 gcagaattca gggtttaaag ccaccatggc ccccccgacc gatgtcagcc tgggggacga
     3121 actccactta gacggcgagg acgtggcgat ggcgcatgcc gacgcgctag acgatttcga
     3181 tctggacatg ttgggggacg gggattcccc aggtccggga tttacccccc acgactccgc
     3241 cccctacggc gctctggata tggccgactt cgagtttgag cagatgttta ccgatgccct
     3301 tggaattgac gagtacggtg ggaagcttct aggtacctct agaagaatat caaattctat
     3361 atcttcaggt cgcgatgatc tctcgccttc gagcagcttg aacggatact cggcgaacga
     3421 aagctgcgat gcgaagaaga gcaagaaggg acctgcgcca cgggtgcaag aggagctgtg
     3481 cctggtttgc ggcgacaggg cctccggcta ccactacaac gccctcacct gtggatcctg
     3541 caaggtgttc tttcgacgca gcgttacgaa gagcgccgtc tactgctgca agttcgggcg
     3601 cgcctgcgaa atggacatgt acatgaggcg aaagtgtcag gagtgccgcc tgaaaaagtg
     3661 cctggccgtg ggtatgcggc cggaatgcgt cgtcccggag aaccaatgtg cgatgaagcg
     3721 gcgcgaagag aaggcccaga aggagaagga caaaatgacc acttcgccga gctctcagca
     3781 tggcggcaat ggcagcttgg cctctggtgg cggccaagac tttgttaaga aggagattct
     3841 tgaccttatg acatgcgagc cgccccagca tgccactatt ccgctactac ctgatgaaat
     3901 attggccaag tgtcaagcgc gcaatatacc ttccttaacg tacaatcagt tggccgttat
     3961 atacaagtta atttggtacc aggatggcta tgagcagcca tctgaagagg atctcaggcg
     4021 tataatgagt caacccgatg agaacgagag ccaaacggac gtcagctttc ggcatataac
     4081 cgagataacc atactcacgg tccagttgat tgttgagttt gctaaaggtc taccagcgtt
     4141 tacaaagata ccccaggagg accagatcac gttactaaag gcctgctcgt cggaggtgat
     4201 gatgctgcgt atggcacgac gctatgacca cagctcggac tcaatattct tcgcgaataa
     4261 tagatcatat acgcgggatt cttacaaaat ggccggaatg gctgataaca ttgaagacct
     4321 gctgcatttc tgccgccaaa tgttctcgat gaaggtggac aacgtcgaat acgcgcttct
     4381 cactgccatt gtgatcttct cggaccggcc gggcctggag aaggcccagc tagtcgaagc
     4441 gatccagagc tactacatcg acacgctacg catttatata ctcaaccgcc actgcggcga
     4501 ctcaatgagc ctcgtcttct acgcaaagct gctctcgatc ctcaccgagc tgcgtacgct
     4561 gggcaaccag aacgccgaga tgtgtttctc actaaagctc aaaaaccgca aactgcccaa
     4621 gttcctcgag gagatctggg acgttcatgc catcccgcca tcggtccagt cgcaccttca
     4681 gattacccag gaggagaacg agcgtctcga gcgggctgag cgtatgcggg catcggttgg
     4741 gggcgccatt accgccggca ttgattgcga ctctgcctcc acttcggcgg cggcagccgc
     4801 ggcccagcat cagcctcagc ctcagcccca gccccaaccc tcctccctga cccagaacga
     4861 ttcccagcac cagacacagc cgcagctaca acctcagcta ccacctcagc tgcaaggtca
     4921 actgcaaccc cagctccaac cacagcttca gacgcaactc cagccacaga ttcaaccaca
     4981 gccacagctc cttcccgtct ccgctcccgt gcccgcctcc gtaaccgcac ctggttcctt
     5041 gtccgcggtc agtacgagca gcgaatacat gggcggaagt gcggccatag gaccaatcac
     5101 gccggcaacc accagcagta tcacggctgc cgttaccgct agctccacca catcagcggt
     5161 accgatgggc aacggagttg gagtcggtgt tggggtgggc ggcaacgtca gcatgtatgc
     5221 gaacgcccag acggcgatgg ccttgatggg tgtagccctg cattcgcacc aagagcagct
     5281 tatcggggga gtggcggtta agtcggagca ctcgacgact gcatagccag tgtgctggaa
     5341 gtaccgagct cgatcgtacc cagcttggag gtgcagggtc gatgcgacgc aatcgtccga
     5401 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     5461 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccggat
     5521 cgggagatgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     5581 tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac
     5641 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     5701 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     5761 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc cactggcccc gtgggttagg
     5821 gacggggtcc ccatggggaa tggtttatgg ttcgtggggg ttattatttt gggcgttgcg
     5881 tggggtcagg tccacgactg gactgagcag acagacccat ggtttttgga tggcctgggc
     5941 atggaccgca tgtactggcg cgacacgaac accgggcgtc tgtggctgcc aaacaccccc
     6001 gacccccaaa aaccaccgcg cggatttctg gcgtgccaag ctagtcgatc gaccaattct
     6061 catgtttgac agcttatcat cgcagatccg tatggtgcac tctcagtaca atctgctctg
     6121 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     6181 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     6241 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgtatct
     6301 gaggggacta gggtgtgttt aggcgaaaag cggggcttcg gttgtacgcg gttaggagtc
     6361 ccctcaggat atagtagttt cgcttttgca tagggagggg gaaatgtagt cttatgcaat
     6421 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     6481 aaagcaccgt gcatgccgat aggtggaagt aaggtggtac gatcgtgcct tattaggaag
     6541 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttccgcattg cagagatatt
     6601 gtatttaagt gcctagctcg atacaataaa cgccatttga ccattcacca cattggagtg
     6661 cacctccaag ctgggtacga tatcaccatg gacaccaaac atttcctgcc gctcgatttc
     6721 tccacccagg tgaactcctc cctcacctcc ccgacggggc gaggctccat ggctgccccc
     6781 tcgctgcacc cgtccctggg gcctggcatc ggctccccgg gacagctgca ttctcccatc
     6841 agcaccctga gctcccccat caacggcatg ggcccgcctt tctcggtcat cagctccccc
     6901 atgggccccc actccatgtc ggtgcccacc acacccaccc tgggcttcag cactggcagc
     6961 ccccagctca gctcacctat gaaccccgtc agcagcagcg aggacatcaa gccccccctg
     7021 ggcctcaatg gcgtcctcaa ggtccccgcc cacccctcag gaaacatggc ttccttcacc
     7081 aagcacatct gcgccatctg cggggaccgc tcctcaggca agcactatgg agtgtacagc
     7141 tgcgaggggt gcaagggctt cttcaagcgg acggtgcgca aggacctgac ctacacctgc
     7201 cgcgacaaca aggactgcct gattgacaag cggcagcgga accggtgcca gtactgccgc
     7261 taccagatgt gcctggccat gggcatgaag cgggaagccg tgcaggagga gcggcagcgt
     7321 ggcaaggacc ggaacgagaa tgaggtggag tcgaccagca gcgccaacga ggacgtgccg
     7381 gtggagagga tcctggaggc tgagctggcc gtggagccca agaccgagac ctacgtggag
     7441 gcaaacgtgg ggctgaaccc cagctcgccg aacgaccctg tcaccaacat ttgccaagca
     7501 gccgacaaac agcttttcac cctggtggag tgggccaagc ggatcccaca cttctcagag
     7561 ctgcccctgg acgaccaggt catcctgctg cgggcaggct ggaatgagct gctcatcgcc
     7621 tccttctccc accgctccat cgccgtgaag gacgggatcc tcctggccac cgggctgcac
     7681 gtccaccgga acagcgccca cagcgcaggg gtgggcgcca tctttgacag ggtgctgacg
     7741 gagcttgtgt ccaagatgcg ggacatgcag atggacaaga cggagctggg ctgcctgcgc
     7801 gccatcgtcc tctttaaccc tgactccaag gggctctcga acccggccga ggtggaggcg
     7861 ctgagggaga aggtctatgc gtccttggag gcctactgca agcacaagta cccagagcag
     7921 ccgggaaggt tcgctaagct cttgctccgc ctgccggctc tgcgctccat cgggctcaaa
     7981 tgcctggaac atctcttctt cttcaagctc atcggggaca cacccattga caccttcctt
     8041 atggagatgc tggaggcgcc gcaccaaatg acttaggcgg ccgctcgagc atgcatctag
     8101 ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact
     8161 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     8221 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     8281 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     8341 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     8401 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     8461 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     8521 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     8581 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     8641 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     8701 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     8761 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     8821 aaaaatgagc tgatttaaca aaaatttaac gcga
//