pLenti6-H2B-mCherry

LOCUS       pLenti6-H2B-mCherry        8245 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentivirus destination vector for stable expression of 
            mCherry-tagged histone H2B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Pemble H, Kumar P, van Haren J, Wittmann T
  TITLE     GSK3-mediated CLASP2 phosphorylation modulates kinetochore dynamics.
  JOURNAL   J Cell Sci. 2017 Apr 15;130(8):1404-1412. doi: 10.1242/jcs.194662. 
            Epub 2017 Feb 23.
  PUBMED    28232523
REFERENCE   2  (bases 1 to 8245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.194662"; journalName: "J Cell Sci"; date: "2017-04-15- 
            15"; volume: "130"; issue: "8"; pages: "1404-1412"
COMMENT     Sequence Label: pLenti6-H2B-mCherry
FEATURES             Location/Qualifiers
     source          1..8245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     182..202
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(239..280)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    333..357
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(378..1088)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(656..675)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     925..943
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      1085..1094
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1107..1484)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     protein_bind    complement(1505..1529)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1625..1649)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(1646..1849)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1675..1695)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1850..2153
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2498..2542)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2727..2960
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3453..3578
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3625..3805
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3806..4032)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4060..4080)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4060..4078)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4099..4115)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4099..4115)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4112..4134)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4123..4139
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4147..4153,4154..4171,4172..4177))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4192..4213
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4330..4347)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4501..5089)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4581..4600)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5260..6051,6052..6120))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5883..5902
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6121..6225)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6251..6706)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6388..6409)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6600..6619
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6833..6855
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6847..6864
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6848..6864
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6874..6893
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6874..6892
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      6913..7048
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7088..7209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7099..7118)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7153..7172
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             7281..7514
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(7601..7999)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(8018..8065)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(join(8113..8245,1..197))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(8127..8245,1..17)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga
       61 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct
      121 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
      181 caccctaact gacacacatt ccacagaatt aattccaaac tcattactaa ccggtacgcg
      241 tagaatcgag accgaggaga gggttaggga taggcttacc ttcgaaccgc gggccctcta
      301 gactcgagcg gccgccactg tgctggatat caaccacttt gtacaagaaa gctgggtcgg
      361 cgcgcccacc cttgcgctta cttgtacagc tcgtccatgc cgccggtgga gtggcggccc
      421 tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccaac
      481 ttgatgttga cgttgtaggc gccgggcagc tgcacgggct tcttggcctt gtaggtggtc
      541 ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca gcctctgctt gatctcgccc
      601 ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg cctcccagcc catggtcttc
      661 ttctgcatta cggggccgtc ggaggggaag ttggtgccgc gcagcttcac cttgtagatg
      721 aactcgccgt cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc gtcctcgaag
      781 ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcaagta gtcggggatg
      841 tcggcggggt gcttcacgta ggccttggag ccgtacatga actgagggga caggatgtcc
      901 caggcgaagg gcagggggcc acccttggtc accttcagct tggcggtctg ggtgccctcg
      961 taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcac ggagccctcc
     1021 atgtgcacct tgaagcgcat gaactccttg atgatggcca tgttatcctc ctcgcccttg
     1081 ctcaccatgg tggcgaccgg tggatcctta gcgctggtgt acttggtgat ggccttagta
     1141 ccctcggaca cggcgtgctt ggccaactcc ccaggcagca gcaggcgcac ggccgtctgg
     1201 atctccctgg aggtgatggt cgagcgcttg ttgtaatgcg ccaggcggga agcctcacct
     1261 gcgatgcgct cgaaaatgtc gttcacaaac gaattcatga tgcccatggc cttggacgaa
     1321 atgccggtgt cagggtggac ctgcttcaga accttgtaca catagatgga atagctctcc
     1381 ttgcggctgc gcttgcgctt cttgccgcct ttcttctgcg ccttagtcac cgccttcttg
     1441 gagccctttt tcggggcggg agcagacttc gctggctctg gcatggtgaa gggggcggcc
     1501 gcggagcctg cttttttgta caaacttgtt gatatctgca gaattccacc acactggact
     1561 agtggatcct ctagagtcgg tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc
     1621 aggcgatctg acggttcact aaacgagctc tgcttatata gacctcccac cgtacacgcc
     1681 taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat
     1741 tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg
     1801 agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag
     1861 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg
     1921 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat
     1981 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
     2041 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
     2101 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
     2161 cccaagctta tcgcagaccc agtcgctagc gttaacgtcg acatcgcaga cccagtcgct
     2221 agcgttaacg tcgacatcga taccgtcgag atccgttcac taatcgaatg gatctgtctc
     2281 tgtctctctc tccaccttct tcttctattc cttcgggcct gtcgggtccc ctcggggttg
     2341 ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa
     2401 gtacagcaaa aactattctt aaacctacca agcctcctac tatcattatg aataatttta
     2461 tataccacag ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt
     2521 ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt aaggagtgta
     2581 ttaagcttgt gtaattgtta atttctctgt cccactccat ccaggtcgtg tgattccaaa
     2641 tctgttccag agatttatta ctccaactag cattccaagg cacagcagtg gtgcaaatga
     2701 gttttccaga gcaaccccaa atccccagga gctgttgatc ctttaggtat ctttccacag
     2761 ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac agatgctgtt
     2821 gcgcctcaat agccctcagc aaattgttct gctgctgcac tataccagac aataattgtc
     2881 tggcctgtac cgtcagcgtc attgacgctg cgcccatagt gcttcctgct gctcccaaga
     2941 acccaaggaa caaagctcct attcccactg ctcttttttc tctctgcacc actcttctct
     3001 ttgccttggt gggtgctact cctaatggtt caatttttac tactttatat ttatataatt
     3061 cacttctcca attgtccctc atatctcctc ctccaggtct gaagatcagc ggccgcttgc
     3121 tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc ttccttggtg
     3181 tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt atataatgat
     3241 ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat ttgtctacag
     3301 ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc cctgcttgcc
     3361 catactatat gttttaattt atattttttc tttccccctg gccttaaccg aattttttcc
     3421 catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc tctctccttc
     3481 tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc
     3541 gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt ccctttcgct
     3601 ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa aagggtctga
     3661 gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag
     3721 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc
     3781 agatctggtc taaccagaga gacccgttta tgtatcgagc taggcactta aatacaatat
     3841 ctctgcaatg cggcaattca gtggttcgtc caatccatgt cagacccgtc tgttgccttc
     3901 ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcacgg tgctttttct
     3961 ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac aagagtattg
     4021 cataagacta cattaagctt gcagctccag cttttgttcc ctttagtgag ggttaattgc
     4081 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4141 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     4201 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4261 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4321 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4381 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4441 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4501 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4561 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4621 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4681 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4741 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4801 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4861 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4921 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4981 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5041 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5101 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5161 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5221 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5281 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5341 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5401 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5461 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5521 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5581 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5641 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5701 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5761 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5821 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5881 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5941 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6001 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6061 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6121 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6181 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6241 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     6301 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     6361 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     6421 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     6481 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     6541 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     6601 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     6661 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     6721 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     6781 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     6841 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
     6901 ggtacgtccc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg
     6961 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
     7021 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggata gctagagcca
     7081 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     7141 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     7201 aaacaagttc ctctcactct ctgatattca tttctttgca agttataaat actgaataat
     7261 aagatgacat gaactactac tgctagagat tttccacact gactaaaagg gtctgaggga
     7321 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
     7381 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
     7441 ctggtctaac cagagagacc cagtacaagc aaaaagcaga tcttgtcttc gttgggagtg
     7501 aattagccct tccagtcccc ccttttcttt taaaaagtgg ctaagatcta cagctgcctt
     7561 gtaagtcatt ggtcttaaag gtaccgagct cgaattgtgc ttagccctcc cacacataac
     7621 cagagggcag caattcacga atcccaactg ccgtcggctg tccatcactg tccttcacta
     7681 tggctttgat cccaggatgc agatcgagaa gcacctgtcg gcaccgtccg caggggctca
     7741 agatgcccct gttctcattt ccgatcgcga cgatacaagt caggttgcca gctgccgcag
     7801 cagcagcagt gcccagcacc acgagttctg cacaaggtcc cccagtaaaa tgatatacat
     7861 tgacaccagt gaagatgcgg ccgtcgctag agagagctgc gctggcgacg ctgtagtctt
     7921 cagagatggg gatgctgttg attgtagccg ttgctctttc aatgagggtg gattcttctt
     7981 gagacaaagg cttggccatg gtttagttcc tcaccttgtc gtattatact atgccgatat
     8041 actatgccga tgattaattg tcaacacgtg ctgatcagat ccgaaaatgg atatacaagc
     8101 tcccgggagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa
     8161 tagctcagag gcagaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg
     8221 gggcggagaa tgggcggaac tgggc
//