| 质粒名称: | pAAVLINK-ASH1L-B |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001933 |
| 产品用途: | Body AAVLINK plasmid for ASH1L expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: |
Promoterless
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ASH1L |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-ASH1L-B
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-ASH1L-B载体质粒基本信息
pAAVLINK-ASH1L-B质粒图谱载体图谱和pAAVLINK-ASH1L-B载体序列质粒序列多克隆位点信息
pAAVLINK-ASH1L-B质粒载体简介
pAAVLINK-ASH1L-B质粒序列载体序列
LOCUS pAAVLINK-ASH1L-B 7039 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7039)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7039
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
protein_bind complement(145..178)
/label=loxJTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..4195
/codon_start=1
/gene="ASH1L"
/gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
/product="histone-lysine N-methyltransferase ASH1L
isoform2"
/EC_number="2.1.1.359; 2.1.1.367"
/label=ASH1L
/note="isoform 2 is encoded by transcript variant
2;probable histone-lysine N-methyltransferase ASH1L;
ASH1-like protein; lysine N-methyltransferase 2H; absent
small and homeotic disks protein 1 homolog; ash1 (absent,
small, or homeotic)-like"
/db_xref="GI:110349788"
/db_xref="CCDS:CCDS1113.2"
/db_xref="GeneID:55870"
/db_xref="HGNC:HGNC:19088"
/db_xref="MIM:607999"
/protein_id="NP_060959.2"
/translation="KVQSKLHNTVSSLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKT
VLNGILSGSPTSLAVLEQTAQQAAGSALGQILPPLLPSSASSSEILPSPICSQSSGTSG
GQSPVSSDAGFVEPSSVPYLHLHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLS
ELTSLKEATPSPISESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHVSLIP
PETSTVLSSLKEKHKHKCKRRNHDYLSYDKMKRQKRKRKKKYPQLRNRQDPDFIAELEE
LISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPSFPLDPLHYIRKPDLKKKRGR
PPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLTAAPIGLGYYGRYPPTLYPP
PPSPSFTTPLPPPSYMHAGHLLLNPAKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVP
PEMAYGWMVEHKHRHRHKHREHRSSEQPQVSMDTGSSRSVLESLKRYRFGKDAVGERYK
HKEKHRCHMSCPHLSPSKSLINREEQWVHREPSESSPLALGLQTPLQIDCSESSPSLSL
GGFTPNSEPASSDEHTNLFTSAIGSCRVSNPNSSGRKKLTDSPGLFSAQDTSLNRLHRK
ESLPSNERAVQTLAGSQPTSDKPSQRPSESTNCSPTRKRSSSESTSSTVNGVPSRSPRL
VASGDDSVDSLLQRMVQNEDQEPMEKSIDAVIATASAPPSSSPGRSHSKDRTLGKPDSL
LVPAVTSDSCNNSISLLSEKLTSSCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLD
HVNKILKAKKLQRQARTGNNFVKRRPGRPRKCPLQAVVSMQAFQAAQFVNPELNRDEEG
AALHLSPDTVTDVIEAVVQSVNLNPEHKKGLKRKGWLLEEQTRKKQKPLPEEEEQENNK
SFNEAPVEIPSPSETPAKPSEPESTLQPVLSLIPREKKPPRPPKKKYQKAGLYSDVYKT
TDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQL
YKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPDDDTRKGCVDDCLNRMIFAECSP
NTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQE
FRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCDPNCEMQKWSVNGVYRI
GLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFEKCRGIIGGKSQRVNGLTSSKNSQ
PMATHKKSGRSKEKRKSKHKLKK"
misc_feature 708..713
/label=SDS
misc_feature 723..728
/label=SDS
misc_feature 903..908
/label=SDS
misc_feature 1467..1472
/label=SDS
misc_feature 1503..1508
/label=SDS
misc_feature 1582..1587
/label=SDS
misc_feature 1613..1618
/label=SDS
misc_feature 1644..1649
/label=SDS
intron 4196..4236
/label=Splicing Donor
misc_feature 4240..4273
/label=lox2272-JT1571
repeat_region 4302..4442
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4302..4431
/label=AAV2 ITR
repeat_region 4303..4443
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4517..4972
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5254..5358
/gene="bla"
/label=AmpR promoter
CDS 5359..6219
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6390..6978
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagca tacattatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag ggaaagtgca atccaaactc cataatacgg
301 tatcaagtct tgctgccaca tttggctcta aattgggcca acagataaat gtcagcaaga
361 aaggaaccat ttatatagga aagagaagag gtcgcaaacc aaaaactgtc ttaaatggta
421 ttctttctgg tagtcctact agccttgctg ttcttgagca aacagctcaa caggcagctg
481 ggtcagcatt aggacagatt cttcccccat tactgccttc atctgctagt agttctgaga
541 ttcttccatc acctatttgc tctcagtctt ctgggactag tggaggtcag agccctgtaa
601 gtagtgatgc aggttttgtt gaacccagtt cagtgccata tttgcattta cactccagac
661 agggcagtat gattcagact cttgcaatga agaaggcctc aaaggggagg aggcggttat
721 ctcctcctac tttgttgcca aattctcctt cgcacttgag tgaactcaca tctctaaaag
781 aagctactcc ttccccaatc agtgagtctc atagtgatga gaccattccc agtgatagtg
841 gaattggaac agataataac agcacatcag acagggcaga gaaattttgt gggcaaaaaa
901 agaggaggca ttcttttgag catgtttctc tgattccccc tgaaacctct acagtgctaa
961 gcagtcttaa agaaaaacat aaacacaaat gtaagcgcag gaatcatgat tacctcagct
1021 atgacaagat gaaaaggcag aaacgaaaac ggaaaaagaa atatccccag cttcgaaata
1081 gacaggatcc agactttatt gcagagctgg aggaactaat aagtcgccta agtgaaattc
1141 ggatcactca tcgaagtcat cattttatcc cccgagatct tctgccaact atctttcgaa
1201 tcaactttaa tagtttctat acacatcctt ctttcccctt agaccctttg cactacattc
1261 gaaaacctga cttaaaaaag aaaagaggga gaccccctaa gatgagggag gcaatggctg
1321 aaatgccttt tatgcacagc cttagttttc ctctttctag tactggattc tatccatctt
1381 atggtatgcc ttactctcct tcacccctta cagctgctcc cataggatta ggttactatg
1441 gaaggtatcc tcccactctt tatccacctc ctccatctcc ttctttcacc acgccacttc
1501 cacctccttc ctatatgcat gctggtcatt tacttctcaa tcctgccaaa taccataaga
1561 aaaagcataa gctacttcga caggaggcct ttcttacaac cagcaggact cccctccttt
1621 ccatgagtac ctaccccagt gttcctcctg agatggccta tggttggatg gttgagcaca
1681 aacacaggca ccgtcacaaa cacagagaac accgttcttc tgaacaaccc caggtttcta
1741 tggacactgg ctcttcccga tctgtcctgg aatctttgaa gcgctataga tttggaaagg
1801 atgctgttgg agagcgatat aagcataagg aaaagcaccg ttgtcacatg tcctgccctc
1861 atctctctcc ttcaaaaagc ttaataaaca gagaggaaca gtgggtccac cgagagcctt
1921 cagaatctag tccattggcc ttgggattgc agacaccttt acagattgac tgttcagaaa
1981 gttctccaag cttatccctt ggaggattca ctcccaactc tgagccagcc agcagtgatg
2041 aacatacaaa ccttttcaca agtgcaatag gcagctgcag agtttcaaac cctaactcca
2101 gtggccggaa gaaattaact gacagccctg gactcttttc tgcacaggac acttcactaa
2161 atcggcttca cagaaaggag tcactgcctt ctaacgaaag ggcagtacag actttggcag
2221 gctcccagcc aacctctgat aaaccctccc agcggccatc agagagcaca aattgtagcc
2281 ctacccggaa aaggtcttca tctgagagta cttcttcaac agtaaacgga gttccctctc
2341 gaagtccaag attagttgct tctggggatg actctgtgga tagtctgctg cagcggatgg
2401 tacaaaatga ggaccaagag cccatggaga aaagtattga tgctgtgatt gcaactgcct
2461 ctgcaccacc ttcttccagt ccaggccgta gccacagcaa ggaccgaacc ctgggaaaac
2521 cagacagcct tttagtgcct gcagtcacaa gtgactcttg caataatagc atctcactcc
2581 tatctgaaaa gttgacaagc agctgttccc cccatcatat caagagaagt gtagtggaag
2641 ctatgcaacg ccaagctcgg aaaatgtgca attacgacaa aatcttggcc acaaagaaaa
2701 acctagacca tgtcaataaa atcttaaaag ccaaaaaact tcaaaggcag gccaggacag
2761 ggaataactt tgtgaaacgt aggccaggtc gacctcggaa atgtcccctt caggctgtcg
2821 tatcaatgca agcattccag gctgctcagt ttgtcaaccc agaattgaac agagacgagg
2881 aaggagcagc actgcacctc agtcctgaca cagttacaga tgtaattgag gctgttgttc
2941 agagtgtaaa tctgaaccca gaacataaaa aggggttgaa gagaaaaggt tggctattgg
3001 aagaacagac cagaaaaaag cagaagccat taccagagga agaagagcaa gagaataata
3061 aaagctttaa tgaagcacca gttgagattc ccagtccttc tgaaacccca gctaaacctt
3121 ctgaacctga aagtaccttg cagcctgtgc tttctctcat cccaagggaa aagaagcccc
3181 cacgtccccc aaagaagaag tatcagaaag cagggctgta ttctgacgtt tacaaaacta
3241 cagacccaaa gagtcgattg atccaattaa agaaagagaa gctggagtat actccaggag
3301 agcatgaata tggattattt ccagcgccca ttcatgttgg aaagtatcta agacaaaaga
3361 gaattgactt ccagcttcct tatgatatcc tttggcagtg gaaacacaat cagctataca
3421 aaaagccaga tgtcccacta tataagaaaa ttcgttcaaa tgtctacgtt gatgtcaaac
3481 ccctttctgg ttacgaagct accacctgta actgtaagaa gccagatgat gacaccagga
3541 agggctgtgt tgatgactgc ctcaatagaa tgatctttgc tgagtgttcc cccaacactt
3601 gcccatgtgg cgagcaatgc tgtaaccaga ggatacagag gcatgaatgg gtgcaatgtc
3661 tagaacgatt tcgagctgag gaaaaaggtt ggggaatcag aaccaaagag cccctaaaag
3721 ctgggcagtt catcattgaa tacctagggg aggtcgtcag tgaacaggag ttcaggaaca
3781 ggatgattga gcagtatcat aatcacagtg accactactg cctgaacctg gatagtggga
3841 tggtgattga cagttaccgc atgggaaatg aggcccgatt catcaaccat agctgtgacc
3901 caaattgtga aatgcagaaa tggtctgtta atggagtata ccggattgga ctctatgctc
3961 ttaaagacat gccagctggg actgaactca cttatgatta taactttcat tccttcaatg
4021 tggaaaaaca gcaactttgt aagtgtggct ttgagaaatg tcgaggaatc atcggaggca
4081 agagtcagcg tgtgaatgga ctcaccagca gcaaaaacag ccagcccatg gccacacaca
4141 aaaaatctgg acggtcaaaa gagaagagaa agtctaagca caagctgaag aaaaggtaag
4201 tatcaaggtt acaagacagg tttaaggaga ccaataagct attattcgta taggatactt
4261 tatacgaagt tatgaattcc acgtgcggac cgagcggccg caggaacccc tagtgatgga
4321 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
4381 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
4441 ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
4501 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
4561 acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc
4621 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
4681 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
4741 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
4801 acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc
4861 tattcttttg atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg
4921 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
4981 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
5041 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
5101 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
5161 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
5221 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
5281 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
5341 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
5401 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
5461 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
5521 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
5581 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
5641 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
5701 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
5761 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
5821 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
5881 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
5941 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
6001 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
6061 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
6121 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
6181 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
6241 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
6301 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
6361 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
6421 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
6481 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
6541 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
6601 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
6661 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
6721 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
6781 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
6841 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
6901 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
6961 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
7021 tggccttttg ctcacatgt
//
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pAAVLINK-ASH1L-B质粒载体应用举例
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