| 质粒名称: | pYK211 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000801 |
| 产品用途: | Mammalian PhCMV-driven constitutive NLS-HNRNPA1-AG-NS3a(H1) expression vector (PhCMV-NLS-HNRNPA1-AG-NS3a(H1)-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | NLS-HNRNPA1-AG-NS3a(H1) |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK211
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK211载体质粒基本信息
pYK211质粒图谱载体图谱和pYK211载体序列质粒序列多克隆位点信息
pYK211质粒载体简介
pYK211质粒序列载体序列
LOCUS pYK211 7998 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7998)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 27, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7998
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 880..900
/label=oDJ173-1
primer_bind 881..991
/label=oYK211_HNRNPA1_F
primer_bind 881..904
/label=oYK432_NHE_F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
primer_bind 881..900
/label=oYK78_HNRNPA1_F
primer_bind 881..900
/label=oYK30-NS3a_Head-F
CDS 908..964
/codon_start=1
/label=NLS2
/translation="PKKKRKVEASASPKKKRKV"
primer_bind 932..979
/label=oYK78_HNRNPA1_F
primer_bind 964..989
/label=oDJ173-1
primer_bind 964..981
/label=oCW33
CDS 965..1924
/codon_start=1
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/product="heterogeneous nuclear ribonucleoprotein A1isoform
b"
/label=HNRNPA1
/note="isoform b is encoded by transcript variant 2;
heterogeneous nuclear ribonucleoprotein B2 protein; nuclear
ribonucleoprotein particle A1 protein; helix-destabilizing
protein; single-strand DNA-binding protein UP1;
heterogeneous nuclear ribonucleoprotein core protein A1;
heterogeneous nuclear ribonucleoprotein A1B protein; hnRNP
core protein A1-like 3; single-strand RNA-binding protein;
putative heterogeneous nuclear ribonucleoprotein A1-like 3;
epididymis secretory sperm binding protein"
/db_xref="CCDS:CCDS44909.1"
/db_xref="GeneID:3178"
/db_xref="HGNC:HGNC:5031"
/db_xref="MIM:164017"
/protein_id="NP_112420.1"
/translation="MSKSESPKEPEQLRKLFIGGLSFETTDESLRSHFEQWGTLTDCVV
MRDPNTKRSRGFGFVTYATVEEVDAAMNARPHKVDGRVVEPKRAVSREDSQRPGAHLTV
KKIFVGGIKEDTEEHHLRDYFEQYGKIEVIEIMTDRGSGKKRGFAFVTFDDHDSVDKIV
IQKYHTVNGHNCEVRKALSKQEMASASSSQRGRSGSGNFGGGRGGGFGGNDNFGRGGNF
SGRGGFGGSRGGGGYGGSGDGYNGFGNDGSNFGGGGSYNDFGNYNNQSSNFGPMKGGNF
GGRSSGPYGGGGQYFAKPRNQGGYGGSSSSSSYGSGRRF"
gene 965..1924
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/label=HNRNPA1
/note="heterogeneous nuclear ribonucleoprotein A1"
/db_xref="GeneID:3178"
/db_xref="HGNC:HGNC:5031"
/db_xref="MIM:164017"
primer_bind 965..993
/label=oYK526_HNRNPA1_F
misc_feature 965..967
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="N-acetylmethionine.
/evidence=ECO:0007744|PubMed:22814378; propagated from
UniProtKB/Swiss-Prot (P09651.5); acetylation site"
misc_feature 968..970
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Phosphoserine./evidence=ECO:0007744|PubMed:20068231,
ECO:0007744|PubMed:23186163; propagated from
UniProtKB/Swiss-Prot (P09651.5); phosphorylation site"
misc_feature 968..970
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="N-acetylserine, in Heterogeneous nuclear
ribonucleoprotein A1, N-terminally processed.
/evidence=ECO:0000269|Ref.9, ECO:0007744|PubMed:20068231,
ECO:0007744|PubMed:22814378; propagated from
UniProtKB/Swiss-Prot (P09651.5); acetylation site"
misc_feature 971..973
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="N6-acetyllysine, alternate.
/evidence=ECO:0007744|PubMed:19608861; propagated from
UniProtKB/Swiss-Prot (P09651.5); acetylation site"
misc_feature 974..1246
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="propagated from UniProtKB/Swiss-Prot
(P09651.5);Region: Globular A domain"
misc_feature 974..976
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Phosphoserine./evidence=ECO:0007744|PubMed:20068231,
ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163;
propagated from UniProtKB/Swiss-Prot (P09651.5);
phosphorylation site"
exon 980..1096
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/inference="alignment:same species:2.1.0"
misc_feature 980..982
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Phosphoserine./evidence=ECO:0007744|PubMed:18669648,
ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692,
ECO:0007744|PubMed:23186163; propagated from
UniProtKB/Swiss-Prot (P09651.5); phosphorylation site"
misc_feature 1028..1030
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Phosphoserine./evidence=ECO:0000250|UniProtKB:P49312
; propagated from UniProtKB/Swiss-Prot (P09651.5);
phosphorylation site"
exon 1097..1243
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/inference="alignment:same species:2.1.0"
exon 1244..1454
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/inference="alignment:same species:2.1.0"
misc_feature 1247..1519
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="propagated from UniProtKB/Swiss-Prot
(P09651.5);Region: Globular B domain"
exon 1455..1547
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/inference="alignment:same species:2.1.0"
misc_feature 1538..1540
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Phosphoserine, by MKNK2.
/evidence=ECO:0000269|PubMed:16111636; propagated from
UniProtKB/Swiss-Prot (P09651.5); phosphorylation site"
misc_feature 1544..1546
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Asymmetric dimethylarginine, alternate.
/evidence=ECO:0000250|UniProtKB:P09867; Dimethylated
arginine, alternate. /evidence=ECO:0007744|PubMed:15782174;
Omega-N-methylarginine, alternate.
/evidence=ECO:0000250|UniProtKB:P49312; propagated from
UniProtKB/Swiss-Prot (P09651.5); methylation site"
exon 1548..1640
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/inference="alignment:same species:2.1.0"
misc_feature 1559..1561
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Phosphoserine./evidence=ECO:0007744|PubMed:21406692;
propagated from UniProtKB/Swiss-Prot (P09651.5);
phosphorylation site"
misc_feature 1580..1582
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Asymmetric dimethylarginine, alternate.
/evidence=ECO:0000269|Ref.9, ECO:0007744|PubMed:24129315;
Dimethylated arginine, alternate.
/evidence=ECO:0007744|PubMed:15782174;
Omega-N-methylarginine, alternate.
/evidence=ECO:0000269|Ref.9, ECO:0007744|PubMed:24129315;
propagated from UniProtKB/Swiss-Prot (P09651.5);
methylation site"
misc_feature 1616..1684
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="propagated from UniProtKB/Swiss-Prot
(P09651.5);Region: RNA-binding RGG-box"
misc_feature 1616..1618
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Asymmetric dimethylarginine, alternate.
/evidence=ECO:0007744|PubMed:24129315;
Omega-N-methylarginine, alternate.
/evidence=ECO:0007744|PubMed:24129315; propagated from
UniProtKB/Swiss-Prot (P09651.5); methylation site"
misc_feature 1637..1639
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Asymmetric dimethylarginine, alternate.
/evidence=ECO:0000269|Ref.9, ECO:0007744|PubMed:24129315;
Dimethylated arginine, alternate.
/evidence=ECO:0007744|PubMed:15782174;
Omega-N-methylarginine, alternate.
/evidence=ECO:0000269|Ref.9, ECO:0007744|PubMed:24129315;
propagated from UniProtKB/Swiss-Prot (P09651.5);
methylation site"
exon 1641..1715
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/inference="alignment:same species:2.1.0"
misc_feature 1658..1660
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Asymmetric dimethylarginine, alternate.
/evidence=ECO:0000250|UniProtKB:P04256;
Omega-N-methylarginine, alternate.
/evidence=ECO:0007744|PubMed:24129315; propagated from
UniProtKB/Swiss-Prot (P09651.5); methylation site"
exon 1717..1871
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/inference="alignment:same species:2.1.0"
misc_feature 1766..1879
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="propagated from UniProtKB/Swiss-Prot
(P09651.5);Region: Nuclear targeting sequence (M9)"
misc_feature 1814..1816
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Omega-N-methylarginine.
/evidence=ECO:0007744|PubMed:24129315; propagated from
UniProtKB/Swiss-Prot (P09651.5); methylation site"
misc_feature 1817..1819
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Phosphoserine./evidence=ECO:0007744|PubMed:23186163;
propagated from UniProtKB/Swiss-Prot (P09651.5);
phosphorylation site"
misc_feature 1856..1858
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="N6-acetyllysine, alternate.
/evidence=ECO:0007744|PubMed:19608861; propagated from
UniProtKB/Swiss-Prot (P09651.5); acetylation site"
misc_feature 1862..1864
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Omega-N-methylarginine.
/evidence=ECO:0007744|PubMed:24129315; propagated from
UniProtKB/Swiss-Prot (P09651.5); methylation site"
exon 1872..1924
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/inference="alignment:same species:2.1.0"
misc_feature 1889..1891
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Phosphoserine./evidence=ECO:0007744|PubMed:23186163;
propagated from UniProtKB/Swiss-Prot (P09651.5);
phosphorylation site"
misc_feature 1892..1894
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Phosphoserine, by MKNK2.
/evidence=ECO:0000269|PubMed:16111636; propagated from
UniProtKB/Swiss-Prot (P09651.5); phosphorylation site"
misc_feature 1895..1897
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Phosphoserine, by MKNK2.
/evidence=ECO:0000269|PubMed:16111636; propagated from
UniProtKB/Swiss-Prot (P09651.5); phosphorylation site"
misc_feature 1898..1900
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Phosphoserine, by MKNK2.
/evidence=ECO:0000269|PubMed:16111636,
ECO:0007744|PubMed:23186163; propagated from
UniProtKB/Swiss-Prot (P09651.5); phosphorylation site"
primer_bind complement(1899..1941)
/label=oDJ173-2
misc_feature 1901..1903
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Phosphoserine./evidence=ECO:0007744|PubMed:21406692,
ECO:0007744|PubMed:23186163; propagated from
UniProtKB/Swiss-Prot (P09651.5); phosphorylation site"
primer_bind complement(1902..1944)
/label=oYK78_HNRNPA1_R
primer_bind complement(1909..1924)
/label=oCW34
misc_feature 1910..1912
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Phosphoserine./evidence=ECO:0007744|PubMed:18669648,
ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163;
propagated from UniProtKB/Swiss-Prot (P09651.5);
phosphorylation site"
misc_feature 1916..1918
/gene="HNRNPA1"
/gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1;
HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
/note="Omega-N-methylarginine.
/evidence=ECO:0007744|PubMed:24129315; propagated from
UniProtKB/Swiss-Prot (P09651.5); methylation site"
misc_feature 1925..2152
/label=Long_Linker
primer_bind 1925..1944
/label=oYK78_AG_ANR1_F
primer_bind 1925..1942
/label=oYK69_AG_ANR1_F
CDS 1925
/codon_start=1
/gene="FUS"
/gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2;
POMP75;TLS"
/product="RNA-binding protein FUS isoform 1"
/label=FUS
/note="isoform 1 is encoded by transcript variant 1;
heterogeneous nuclear ribonucleoprotein P2; fused in
sarcoma; fusion gene in myxoid liposarcoma; 75 kDa
DNA-pairing protein; RNA-binding protein FUS; oncogene TLS;
fus-like protein; oncogene FUS; translocated in liposarcoma
protein"
/db_xref="CCDS:CCDS10707.1"
/db_xref="GeneID:2521"
/db_xref="HGNC:HGNC:4010"
/db_xref="MIM:137070"
/protein_id="NP_004951.1"
/translation=""
misc_feature 2153..2827
/label=AG
primer_bind 2601..2611
/label=oSCL1104
primer_bind complement(2815..2835)
/label=oYK65-AG-R
misc_feature 2828..2878
/label=Short_Linker
primer_bind complement(2854..2878)
/label=oYK33-35-PB1-AG-R
primer_bind 2859..2899
/label=oYK153_NS3a_F
CDS 2879..3466
/codon_start=1
/label=NS3a H1
/translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
SRSLTPCTCGSSDLYLVTRHADVIPVRGRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
primer_bind 2879..2914
/label=oSCL1082
primer_bind 2879..2899
/label=oyk03-03-F
primer_bind 2879..2899
/label=oYK04-ns3a-F
primer_bind 2879..2897
/label=oYK25-NS3a-F
primer_bind 2879..2896
/label=oYK30-NS3a_Head-F
primer_bind 2896..2954
/label=oSCL1083
primer_bind 2935..2993
/label=oSCL1084
primer_bind 2972..3030
/label=oSCL1085
primer_bind 3009..3067
/label=oSCL1086
primer_bind complement(3037..3056)
/label=oSCL1106
primer_bind 3038..3076
/label=oSCL1104
primer_bind complement(3273..3331)
/label=oSCL1105
primer_bind complement(3313..3384)
/label=oSCL1087
primer_bind 3315..3336
/label=oSCL1107
primer_bind 3366..3384
/label=oSCL1088
primer_bind complement(3447..3472)
/label=oYK433_NS3a2_R
primer_bind complement(3448..3495)
/label=oSCL1089
primer_bind complement(3451..3498)
/label=oYK30-NS3a_head-R
primer_bind complement(3467..3515)
/label=oYK03-03-R
primer_bind complement(3467..3486)
/label=oSCL1130
primer_bind complement(3475..3495)
/label=olyl382
primer_bind complement(3496..3518)
/label=oYK57-MCP-mCherry-R
primer_bind complement(3496..3515)
/label=oYK25-NS3a-R
primer_bind complement(3519..3539)
/label=oCW34
primer_bind complement(3520..3539)
/label=oSCL1089
terminator 3592..3819
/label=bGH PA
misc_feature 3865..4293
/label=f1 origin
misc_feature 4298..4641
/label=SV40 early promoter and origin
misc_feature 4487..4564
/label=SV40 ORI
CDS 4706..5497
/label=Kan/neoR
misc_feature 5671..5801
/label=SV40 early polyadenylation signal
primer_bind 5775..5794
/label=olyl382
primer_bind complement(5837..5857)
/label=M13-rev
misc_binding complement(5863..5885)
/label=LacO
promoter complement(5890..5919)
/label=lac
primer_bind 6034..6045
/label=oYK69_AG_ANR1_F
misc_feature complement(6184..6857)
/label=pUC origin
CDS complement(7005..7664)
/label=AmpR
primer_bind 7321..7332
/label=oyk03-03-F
primer_bind 7321..7332
/label=oYK04-ns3a-F
primer_bind 7321..7332
/label=oYK153_NS3a_F
promoter complement(7904..7932)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 caccatgccc aagaagaagc gcaaggtgga agctagcgct tccccgaaga aaaagcggaa
961 agtcatgtct aagtcagagt ctcctaaaga gcccgaacag ctgaggaagc tcttcattgg
1021 agggttgagc tttgaaacaa ctgatgagag cctgaggagc cattttgagc aatggggaac
1081 gctcacggac tgtgtggtaa tgagagatcc aaacaccaag cgctccaggg gctttgggtt
1141 tgtcacatat gccactgtgg aggaggtgga tgcagctatg aatgcaaggc cacacaaggt
1201 ggatggaaga gttgtggaac caaagagagc tgtctccaga gaagattctc aaagaccagg
1261 tgcccactta actgtgaaaa agatatttgt tggtggcatt aaagaagaca ctgaagaaca
1321 tcacctaaga gattattttg aacagtatgg aaaaattgaa gtgattgaaa tcatgactga
1381 ccgaggcagt ggcaagaaaa ggggctttgc ctttgtaacc tttgacgacc atgactccgt
1441 ggataagatt gtcattcaga aataccatac tgtgaatggc cacaactgtg aagttagaaa
1501 agccctgtca aagcaagaga tggctagtgc ttcatccagc caaagaggtc gaagtggttc
1561 tggaaacttt ggtggtggtc gtggaggtgg tttcggtggg aatgacaact tcggtcgtgg
1621 aggaaacttc agtggtcgtg gtggctttgg tggcagccgt ggtggtggtg gatatggtgg
1681 cagtggggat ggctataatg gatttggtaa tgatggaagc aattttggag gtggtggaag
1741 ctacaatgat tttgggaatt acaacaatca gtcttcaaat tttggaccca tgaagggagg
1801 aaattttgga ggcagaagct ctggccccta tggcggtgga ggccaatact ttgcaaaacc
1861 acgaaaccaa ggtggctatg gcggttccag cagcagcagt agctatggca gtggcagaag
1921 atttggttct gggagtggcg gtagtggtgc tggaggcagc gcaggctctg gagcaggcgg
1981 cagtgccgga agtggcgctg gagggtctgc cggatctgga gccggtggtt ctgccggctc
2041 cggcgctggt gggagcgctt ccggaagtag tcccggtaat tccgctgacg gcggcggagg
2101 atcgggtggt agtggtggtt caggaggagg atcgacccaa ggaggatcca tggtgagcgt
2161 gatcaagccc gagatgaaga tcaagctgtg catgaggggc accgtgaacg gccacaactt
2221 cgtgatcgag ggcgagggca agggcaaccc ctacgagggc acccagatcc tggacctgaa
2281 cgtgaccgag ggcgcccccc tgcccttcgc ctacgacatc ctgaccaccg tgttccagta
2341 cggcaacagg gccttcacca agtaccccgc cgacatccag gactacttca agcagacctt
2401 ccccgagggc taccactggg agaggagcat gacctacgag gaccagggca tctgcaccgc
2461 caccagcaac atcagcatga ggggcgactg cttcttctac gacatcaggt tcgacggcgt
2521 gaacttcccc cccaacggcc ccgtgatgca gaagaagacc ctgaagtggg agcccagcac
2581 cgagaagatg tacgtgaggg acggcgtgct gaagggcgac gtgaacatgg ccctgctgct
2641 ggagggcggc ggccactaca ggtgcgactt caagaccacc tacaaggcca agaaggacgt
2701 gaggctgccc gactaccact tcgtggacca caggatcgag atcctgaagc acgacaagga
2761 ctacaacaag gtgaagctgt acgagaacgc cgtggccagg tacagcatgc tgcccagcca
2821 ggccaagggt accggaactg cagcagagaa ttcgggaaac tcgagaacaa agcttgaaaa
2881 gaagaagggc agcgtggtga tcgtgggcag aatcaacctg agcggcgaca ccgcctacag
2941 ccagcagacc agaggcctgg agggctgcca ggagaccagc cagaccggca gagacaagaa
3001 ccaggtggag ggcgaggtgc aggtggtgag caccgccacc cagagcttcc tggccaccag
3061 catcaacggc gtgctgtgga ccgtgtacca cggcgccggc accagaacca tcgccagccc
3121 caagggcccc gtgacccaga tgtacacgaa cgtggacaag gacttagtgg gctggcaggc
3181 cccccaaggc agcagaagcc tgaccccctg cacctgcggc agcagcgacc tgtacctggt
3241 gaccagacac gccgacgtga tccccgtgag aggaagaggc gacagcagag gcagcctgct
3301 gagccccaga cccatcagct atctgaaagg ctcaagcggc ggccccctgt tgtgtcccgc
3361 cggccatgcc gtgggcatct tcagagccgc cgtgagcacc agaggcgtgg ccaaggccgt
3421 ggacttcatc cccgtggaga gcctggagac caccatgaga agccccgcta gctagaaatt
3481 ctgcagatat ccagcggatc cactagtcca gtgtggtgga attctgcaga tatccagcac
3541 agtggcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
3601 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
3661 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
3721 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
3781 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
3841 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
3901 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
3961 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
4021 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
4081 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
4141 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
4201 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
4261 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
4321 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
4381 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
4441 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
4501 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
4561 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
4621 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
4681 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
4741 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
4801 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
4861 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
4921 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
4981 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
5041 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
5101 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
5161 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
5221 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
5281 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
5341 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
5401 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
5461 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
5521 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
5581 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
5641 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
5701 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
5761 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
5821 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
5881 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
5941 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
6001 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
6061 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
6121 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
6181 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
6241 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
6301 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
6361 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
6421 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
6481 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
6541 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
6601 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
6661 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
6721 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
6781 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
6841 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
6901 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
6961 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
7021 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
7081 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
7141 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
7201 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
7261 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
7321 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
7381 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
7441 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
7501 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
7561 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
7621 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
7681 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
7741 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
7801 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
7861 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
7921 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
7981 aaagtgccac ctgacgtc
//
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