| 质粒名称: | pAAV-CaMKII-monArch-PP-KGC-GFP-Kv2.1-ER2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001084 |
| 产品用途: | voltage imaging |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CaMKII |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10, DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | monArch, GFP |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Engineering of Genetically Encoded Bright Near-Infrared Fluorescent Voltage Indicator
Xiao X., Yang A., Zhang H., Park D., Wang Y., Szabo B., Boyden E.S., Piatkevich K.D.
Int. J. Mol. Sci. 2025, Vol. 26, Page 1442 26, 1442 (2025)
|
pAAV-CaMKII-monArch-PP-KGC-GFP-Kv2.1-ER2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-CaMKII-monArch-PP-KGC-GFP-Kv2.1-ER2载体质粒基本信息
pAAV-CaMKII-monArch-PP-KGC-GFP-Kv2.1-ER2质粒图谱载体图谱和pAAV-CaMKII-monArch-PP-KGC-GFP-Kv2.1-ER2载体序列质粒序列多克隆位点信息
pAAV-CaMKII-monArch-PP-KGC-GFP-Kv2.1-ER2质粒载体简介
pAAV-CaMKII-monArch-PP-KGC-GFP-Kv2.1-ER2质粒序列载体序列
LOCUS pAAV-CaMKII-monArch-PP-KGC-GFP-Kv2.1-ER2 6870 bp DNA circular SYN 19-JUN-2020
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6870)
AUTHORS Kiryl Piatkevich
TITLE Direct Submission
JOURNAL Exported Aug 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6870
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 34..223
/label=ITR
promoter 237..1527
/label=CAMKII
primer_bind 1449..1468
/label=CaMK-Fw-Seq
primer_bind 1512..1557
/label=0609-CaMKII-BamHI-monArch-Fw
misc_feature 1535
/label=MCS
/note="multiple cloning site"
regulatory 1536..1541
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1542..2300
/codon_start=1
/label=monArch
/translation="MVSIALQAGYDLLGDGRPEILWLGIGTLLILIGTFYFLVRGWGVT
NKDAREYYTVTILVSGIAGAAYLLMFFGIGLAEVSVGGEMLVIYYARYAEWLFCTPLLL
LDLALLAKVDRVIIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYVLA
TSLRSAAKERGPEVASTFNILTALVLVLWTAYPIIWIIGTEGAGVVGLGIETLLFMVLD
VTGKVGFGFILLRSRAILGVTEAPEPSAGAD"
misc_feature 2265..2267
/label=D?
primer_bind complement(2279..2327)
/label=0609-KGC-PP-monArch-Rv
primer_bind 2279..2327
/label=0609-monArch-PP-KGC-Fw
CDS 2307..2366
/codon_start=1
/label=KGC
/translation="KSRITSEGEYIPLDQIDINV"
CDS 2367..3079
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELY"
CDS 3081..3097
/codon_start=1
/label=Linker
/translation="GGSGG"
CDS 3099..3293
/codon_start=1
/label=Kv2.1
/translation="QSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVIDMRS
MSSIDSFISCATDFPEATRF"
CDS 3294..3299
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="YK"
CDS 3300..3320
/codon_start=1
/label=ER2
/translation="FCYENEV"
primer_bind complement(3316..3347)
/label=0609-WPRE-EcoRI-ER2-Rv
misc_feature 3350..3937
/label=WPRE
misc_feature complement(3976..4095)
/label=SV40_PA_terminator
misc_feature complement(4203..4392)
/label=ITR
misc_feature complement(4613..5232)
/label=pBR322_origin
misc_feature complement(5387..6247)
/label=Ampicilin
misc_feature 6289..6317
/label=Ampr promoter
ORIGIN
1 gcgatcggtg cgggcctctt cgctattacg ccagctgcgc gctcgctcgc tcactgaggc
61 cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg
121 agcgcgcaga gagggagtgg ccaactccat cactaggggt tccttgtagt taatgattaa
181 cccgccatgc tacttatcta cgtagccatg ctctaggaag atcgtaggtt aattaacatt
241 atggccttag gtcacttcat ctccatgggg ttcttcttct gattttctag aaaatgagat
301 gggggtgcag agagcttcct cagtgacctg cccagggtca catcagaaat gtcagagcta
361 gaacttgaac tcagattact aatcttaaat tccatgcctt gggggcatgc aagtacgata
421 tacagaagga gtgaactcat tagggcagat gaccaatgag tttaggaaag aagagtccag
481 ggcagggtac atctacacca cccgcccagc cctgggtgag tccagccacg ttcacctcat
541 tatagttgcc tctctccagt cctaccttga cgggaagcac aagcagaaac tgggacagga
601 gccccaggag accaaatctt catggtccct ctgggaggat gggtggggag agctgtggca
661 gaggcctcag gaggggccct gctgctcagt ggtgacagat aggggtgaga aagcagacag
721 agtcattccg tcagcattct gggtctgttt ggtacttctt ctcacgctaa ggtggcggtg
781 tgatatgcac aatggctaaa aagcagggag agctggaaag aaacaaggac agagacagag
841 gccaagtcaa ccagaccaat tcccagagga agcaaagaaa ccattacaga gactacaagg
901 gggaagggaa ggagagatga attagcttcc cctgtaaacc ttagaaccca gctgttgcca
961 gggcaacggg gcaatacctg tctcttcaga ggagatgaag ttgccagggt aactacatcc
1021 tgtctttctc aaggaccatc ccagaatgtg gcacccacta gccgttacca tagcaactgc
1081 ctctttgccc cacttaatcc catcccgtct gttaaaaggg ccctatagtt ggaggtgggg
1141 gaggtaggaa gagcgatgat cacttgtgga ctaagtttgt tcacatcccc ttctccaacc
1201 ccctcagtac atcaccctgg gagaacaagg tccacttgct tctgggccca cacagtcctg
1261 cagtattgtg tatataaggc cagggcaacg gaggagcagg ttttgaagtg aaaggcaggc
1321 aggtgttggg gaggcagtta ccggggcaac gggaacaggg cgtttcggag gtggttgcca
1381 tggggacctg gatgctgacg aaggctcgcg aggctgtgag cagccacagt gccctgctca
1441 gaagccccaa gctcgtcaat caagctggtt ctccatttgc actcaggagc acgggcaggc
1501 gagtggcccc tagttctggg ggcagcgggg gatccgccac catggtttct atcgcactgc
1561 aggctggtta tgacctcctc ggagatggta gacctgaaat cctgtggctc ggaattggta
1621 cactgctgat actgatcgga accttctact tcctggttag agggtgggga gtgactaaca
1681 aggatgcacg ggagtactac acagtgacca tcctggtcag cggtattgcc ggtgcagctt
1741 atctgttaat gttctttggt atcggcctcg cagaggtgtc agttggagga gagatgctgg
1801 tcatctacta cgcaagatac gcagagtggc tgttctgcac accactgctg ctgctggacc
1861 tggcactcct ggccaaagtt gatagagtga tcatcggcac tctcgttggc gtcgatgctc
1921 tgatgattgt cacaggcctg ataggagcac tgtcacatac tgccattgcc agatactctt
1981 ggtggctgtt ctcaactatc tgtatgatcg tcgttctgta tgtcctggcc acttctctgc
2041 ggtcagccgc taaggagagg ggcccagagg ttgcctcaac cttcaacatc ctcaccgcac
2101 tggttctggt cctgtggact gcttacccta tcatctggat tatcggcacc gagggtgccg
2161 gagttgtggg cctgggaatc gaaactctgc tgttcatggt gctggacgtc accggcaagg
2221 tgggcttcgg gtttatcctg ctcaggtcaa gagccattct gggagttaca gaagcacctg
2281 aaccttcagc tggagctgat ccccccaaga gcaggatcac cagcgagggc gagtacatcc
2341 ccctggacca gatcgacatc aacgtggtga gcaagggcga ggagctgttc accggggtgg
2401 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
2461 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatttgc accaccggca
2521 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
2581 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
2641 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
2701 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
2761 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
2821 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
2881 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
2941 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
3001 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
3061 gcatggacga gctgtacaag ggtgggagcg gaggtaccca gagccagcct atcctgaaca
3121 ccaaggagat ggcccctcag agcaagcctc ctgaggagct ggagatgagc agcatgccta
3181 gccctgtggc ccctctgcct gccaggaccg agggcgtgat cgacatgagg agcatgagca
3241 gcatcgacag cttcatcagc tgcgccaccg acttccctga ggccaccagg ttctacaagt
3301 tctgctacga gaacgaggtg taagaattcg atatcaagct tatcgataat caacctctgg
3361 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
3421 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
3481 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
3541 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
3601 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
3661 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
3721 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
3781 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
3841 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
3901 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cccgggcggc
3961 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
4021 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
4081 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
4141 gggagatgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata
4201 aggatcttcc tagagcatgg ctacgtagat aagtagcatg gcgggttaat cattaactac
4261 aaggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
4321 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
4381 cgagcgcgca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
4441 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
4501 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
4561 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
4621 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
4681 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
4741 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
4801 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
4861 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
4921 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
4981 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
5041 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
5101 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
5161 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
5221 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
5281 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
5341 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
5401 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
5461 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
5521 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
5581 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
5641 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
5701 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
5761 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
5821 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
5881 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
5941 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
6001 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
6061 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
6121 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
6181 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
6241 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
6301 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
6361 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
6421 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
6481 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
6541 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
6601 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
6661 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
6721 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
6781 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
6841 gccattcagg ctgcgcaact gttgggaagg
//
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