pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KIF13B-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KIF13B-FLAG              7236 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7236)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3949
                     /codon_start=1
                     /gene="KIF13B"
                     /gene_synonym="GAKIN"
                     /product="kinesin-like protein KIF13B"
                     /label=KIF13B
                     /note="kinesin 13B; guanylate kinase associated kinesin; 
                     kinesin-like protein GAKIN"
                     /db_xref="GI:46852172"
                     /db_xref="CCDS:CCDS55217.1"
                     /db_xref="GeneID:23303"
                     /db_xref="HGNC:HGNC:14405"
                     /db_xref="MIM:607350"
                     /protein_id="NP_056069.2"
                     /translation="GQSRRVQVEVKSVQESGTLPLMEECILSVGIGCVKVRPLRAPRTH
                     ETFHEEEEDMDSYQDRDLERLRRKWLNALTKRQEYLDQQLQKLVSKRDKTEDDADREAQ
                     LLEMRLTLTEERNAVMVPSAGSGIPGAPAEWTPVPGMETHIPVIFLDLNADDFSSQDNL
                     DDPEAGGWDATLTGEEEEEFFELQIVKQHDGEVKAEASWDSAVHGCPQLSRGTPVDERL
                     FLIVRVTVQLSHPADMQLVLRKRICVNVHGRQGFAQSLLKKMSHRSSIPGCGVTFEIVS
                     NIPEDAQGVEEREALARMAANVENPASADSEAYIEKYLRSVLAVENLLTLDRLRQEVAV
                     KEQLTGKGKLSRRSISSPNVNRLSGSRQDLIPSYSLGSNKGRWESQQDVSQTTVSRGIA
                     PAPALSVSPQNNHSPDPGLSNLAASYLNPVKSFVPQMPKLLKSLFPVRDEKRGKRPSPL
                     AHQPVPRIMVQSASPDIRVTRMEEAQPEMGPDVLVQTMGAPALKICDKPAKVPSPPPVI
                     AVTAVTPAPEAQDGPPSPLSEASSGYFSHSVSTATLSDALGPGLDAAAPPGSMPTAPEA
                     EPEAPISHPPPPTAVPAEEPPGPQQLVSPGRERPDLEAPAPGSPFRVRRVRASELRSFS
                     RMLAGDPGCSPGAEGNAPAPGAGGQALASDSEEADEVPEWLREGEFVTVGAHKTGVVRY
                     VGPADFQEGTWVGVELDLPSGKNDGSIGGKQYFRCNPGYGLLVRPSRVRRATGPVRRRS
                     TGLRLGAPEARRSATLSGSATNLASLTAALAKADRSHKNPENRKSWAS"
     misc_feature    2145..2150
                     /label=SDS
     misc_feature    2527..2532
                     /label=SDS
     misc_feature    2600..2605
                     /label=SDS
     misc_feature    2988..2993
                     /label=SDS
     CDS             3956..4021
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4025..4456
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4169..4174
                     /label=SDS
     repeat_region   4499..4639
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4499..4628
                     /label=AAV2 ITR
     repeat_region   4500..4640
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4714..5169
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5451..5555
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5556..6416
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6587..7175
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggggc agtcccggag
     1561 agttcaagtc gaagtgaagt cagtgcagga atctgggact ttaccactga tggaagaatg
     1621 tatactgtct gttggcattg gatgtgtcaa agttagaccg ctcagagccc ccagaacaca
     1681 tgagaccttc catgaggaag aggaagacat ggacagctac caggatcgag atttagagag
     1741 acttcgtaga aaatggctaa atgcattaac aaaacgtcag gagtacttgg atcaacaatt
     1801 gcaaaagctt gtcagtaaac gtgataaaac agaggatgat gctgaccgtg aagcgcagct
     1861 tctggagatg cggttgaccc taactgagga gaggaacgcg gtgatggtcc cctctgctgg
     1921 cagtggtatt ccaggggccc cagcagaatg gaccccagta cctgggatgg agacacacat
     1981 tcctgttata ttcctggact taaatgctga tgatttcagc tctcaggata atcttgatga
     2041 cccagaagct ggtggatggg atgcgacctt gactggggaa gaagaagagg agttctttga
     2101 attgcagatt gtgaagcagc atgatgggga ggtgaaagca gaagcctcct gggactccgc
     2161 ggtgcatggc tgccctcagc tcagcagggg cacgcccgtg gacgagcggt tgttcctgat
     2221 cgtgcgcgtg acggtccagc tcagccaccc tgctgacatg caactggtgt tacgcaagag
     2281 aatctgtgtc aatgttcacg gccgccaggg ttttgcacag agtctcctaa aaaagatgtc
     2341 tcatcgaagt tctattcctg gctgtggagt gacttttgaa attgtctcca atattccaga
     2401 ggatgcccag ggagtggaag aacgggaagc attagcaaga atggcagcca atgttgaaaa
     2461 cccagcttct gctgactcgg aggcttatat tgaaaagtac ctcaggagcg tgctggctgt
     2521 agaaaacctc ctgactttag atcgtctgcg ccaggaagtt gcagtgaagg aacagttaac
     2581 aggaaaagga aagttgagca ggaggagtat cagttctcca aatgtgaaca gattgtctgg
     2641 aagccgacaa gatctcattc catcatacag tctaggcagc aacaagggcc ggtgggaaag
     2701 tcagcaggat gtatcccaaa ccacagtttc cagaggaata gctcctgccc ccgccctctc
     2761 tgtttctccc caaaataacc attctccaga tccaggactc agtaaccttg cagcatccta
     2821 cttgaatcct gtcaaatcct tcgtgccgca aatgccaaag ctcctcaagt ctctctttcc
     2881 cgtccgcgat gagaagaggg gcaagcggcc gtctcccctc gcacaccagc ccgtgccccg
     2941 catcatggtg cagtcagcca gcccggacat cagggtgacc aggatggagg aggctcagcc
     3001 ggagatgggc cctgacgtgc tggtgcagac gatgggggcc ccggccttga agatctgcga
     3061 caaacctgcc aaagtgcctt ccccaccgcc tgtcatagct gtcacagcgg tcaccccggc
     3121 tccggaggca caggacgggc cccccagccc cctgagtgaa gcctctagcg ggtacttctc
     3181 ccacagcgtc tccaccgcga ccctgtcgga cgccctgggc cccggcctgg acgctgcggc
     3241 cccgccgggg tccatgccca ccgcccctga ggccgagccc gaggcgccca tcagccaccc
     3301 cccaccgccc acggccgtcc ccgccgagga gccccctggc ccccagcagc tcgtgagccc
     3361 cggtcgggag cgccccgacc tcgaggcccc ggcgcccggc tccccgttcc gcgtccggag
     3421 ggtgcgggcc tcggagttgc gctccttctc gcgcatgctg gctggggacc ccggctgctc
     3481 cccgggggcc gaggggaatg cgccggcccc gggcgccggg ggacaggccc tggcctctga
     3541 ttccgaggaa gctgacgagg tcccggagtg gctccgagag ggcgagttcg tcaccgtggg
     3601 cgcccacaaa acgggcgtgg tgagatacgt ggggcctgcc gacttccaag agggcacgtg
     3661 ggtcggcgtg gagctcgacc tgccctcagg taagaatgac ggttccatcg gcgggaagca
     3721 gtacttcagg tgtaaccctg gctacgggct gctggtcagg cccagccggg tccgcagggc
     3781 cacgggccct gtgcggcggc gcagcacagg actccggctg ggtgcccccg aggcccgccg
     3841 gagcgccacc ctctcgggct ccgccaccaa cctggcctcg ctgacagctg ccctggccaa
     3901 ggccgacagg agccacaaga accctgagaa ccggaaatcc tgggccagct gtacagacta
     3961 caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa
     4021 gtaactcgag ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     4081 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     4141 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt agttcttgcc
     4201 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4261 actgacaatt ccgtggtgtt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     4321 atctagcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     4381 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat
     4441 gtgggaggtt ttttaaagat ctggagaccg gtgtggcacg tgcggaccga gcggccgcag
     4501 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     4561 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     4621 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     4681 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
     4741 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg
     4801 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4861 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4921 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4981 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5041 tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt
     5101 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     5161 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     5221 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5281 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5341 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     5401 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5461 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5521 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5581 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5641 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5701 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5761 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5821 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5881 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5941 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6001 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6061 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6121 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6181 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6241 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6301 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6361 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6421 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6481 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6541 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6601 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6661 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6721 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6781 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6841 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6901 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6961 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7021 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7081 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7141 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7201 ggttcctggc cttttgctgg ccttttgctc acatgt
//