| 质粒名称: | pAAVLINK-U6-sgRNA-EF1alpha-5'-YE1-BE4max |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001913 |
| 产品用途: | 5' AAVLINK plasmid for YE1-BE4max expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1alpha |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | YE1-BE4max |
| 物种来源: | 未知 |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-U6-sgRNA-EF1alpha-5'-YE1-BE4max
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-U6-sgRNA-EF1alpha-5'-YE1-BE4max载体质粒基本信息
pAAVLINK-U6-sgRNA-EF1alpha-5'-YE1-BE4max质粒图谱载体图谱和pAAVLINK-U6-sgRNA-EF1alpha-5'-YE1-BE4max载体序列质粒序列多克隆位点信息
pAAVLINK-U6-sgRNA-EF1alpha-5'-YE1-BE4max质粒载体简介
pAAVLINK-U6-sgRNA-EF1alpha-5'-YE1-BE4max质粒序列载体序列
LOCUS pAAVLINK-U6-sgRNA-EF1alpha-5'-YE1-BE4max 7464 bp DNA circular SYN 22-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS 1913-pAAVLINK-U6-sgRNA-EF1alpha-5'-YE1-BE4max
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7464)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 15, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT Alias: 7464bp
FEATURES Location/Qualifiers
source 1..7464
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 145..393
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA
(Domitrovich & Kunkel, 2003)"
promoter 145..385
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
misc_RNA 415..490
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 504..1046
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 533..744
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 757..1025
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 851..856
/label=SDS
CDS 1095..1115
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1116..1799
/codon_start=1
/label=YE1-rA1
/translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSYSPCGECSRAITEFLS
RYPHVTLFIYIARLYHHADPENRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
WATGLK"
CDS 1896..4598
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKLKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTK"
CDS 1896..4457
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKLKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNK"
CDS 1896..3611
/codon_start=1
/product="N-terminal portion of Streptococcus pyogenes Cas9
(Zetsche et al., 2015)"
/label=Cas9(N)
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKLKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
intron 4599..4639
/label=Splicing Donor
misc_feature 4649..4682
/label=lox2272-JT15
repeat_region 4727..4867
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4727..4856
/label=AAV2 ITR
repeat_region 4728..4868
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4942..5397
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5679..5783
/gene="bla"
/label=AmpR promoter
CDS 5784..6644
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6815..7403
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgagggc ctatttccca tgattccttc atatttgcat
181 atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata
241 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
301 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
361 gctttatata tcttgtggaa aggacgaaac accgccaggt tccatgggat gctcgtttta
421 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc
481 gagtcggtgc tttttttgta caggatctgc gatcgctccg gtgcccgtca gtgggcagag
541 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
601 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
661 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
721 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
781 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
841 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
901 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
961 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
1021 agatccaagc tgtgaccggc gcctaccgta cggccaccat gaaacggaca gccgacggaa
1081 gcgagttcga gtcaccaaag aagaagcgga aagtctcctc agagactggg cctgtcgccg
1141 tcgatccaac cctgcgccgc cggattgaac ctcacgagtt tgaagtgttc tttgaccccc
1201 gggagctgag aaaggagaca tgcctgctgt acgagatcaa ctggggaggc aggcactcca
1261 tctggaggca cacctctcag aacacaaata agcacgtgga ggtgaacttc atcgagaagt
1321 ttaccacaga gcggtacttc tgccccaata ccagatgtag catcacatgg tttctgagct
1381 actccccttg cggagagtgt agcagggcca tcaccgagtt cctgtccaga tatccacacg
1441 tgacactgtt tatctacatc gccaggctgt atcaccacgc agacccagag aataggcagg
1501 gcctgcgcga tctgatcagc tccggcgtga ccatccagat catgacagag caggagtccg
1561 gctactgctg gcggaacttc gtgaattatt ctcctagcaa cgaggcccac tggcctaggt
1621 acccacacct gtgggtgcgc ctgtacgtgc tggagctgta ttgcatcatc ctgggcctgc
1681 ccccttgtct gaatatcctg cggagaaagc agccccagct gaccttcttt acaatcgccc
1741 tgcagtcttg tcactatcag aggctgccac cccacatcct gtgggccaca ggcctgaagt
1801 ctggaggatc tagcggagga tcctctggca gcgagacacc aggaacaagc gagtcagcaa
1861 caccagagag cagtggcggc agcagcggcg gcagcgacaa gaagtacagc atcggcctgg
1921 ccatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca
1981 aaaaattaaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag
2041 ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa
2101 gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga
2161 tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg
2221 ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg
2281 agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg
2341 acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga
2401 tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc
2461 agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg
2521 ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc
2581 ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc
2641 ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca
2701 cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt
2761 ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca
2821 ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc
2881 aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga
2941 ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg
3001 aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc
3061 tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca
3121 tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt
3181 acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct
3241 actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg
3301 aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga
3361 gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca
3421 agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg
3481 tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg
3541 acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca
3601 agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct
3661 ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg
3721 aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag
3781 agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc
3841 agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca
3901 tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca
3961 acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga
4021 aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca
4081 gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag
4141 tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca
4201 cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag
4261 agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga
4321 agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca
4381 tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg
4441 actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg
4501 tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca
4561 agctgattac ccagagaaag ttcgacaatc tgaccaaggt aagtatcaag gttacaagac
4621 aggtttaagg agaccaataa gcgctagcaa ttattcgtat aggatacttt atacgaagtt
4681 attaagttat ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg
4741 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
4801 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
4861 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
4921 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
4981 tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt
5041 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
5101 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
5161 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
5221 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct
5281 cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg
5341 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
5401 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
5461 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
5521 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
5581 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
5641 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
5701 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
5761 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
5821 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
5881 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
5941 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
6001 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
6061 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
6121 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
6181 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
6241 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
6301 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
6361 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
6421 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
6481 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
6541 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
6601 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
6661 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
6721 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
6781 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
6841 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
6901 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
6961 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
7021 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
7081 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
7141 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
7201 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
7261 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
7321 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
7381 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
7441 tttgctggcc ttttgctcac atgt
//
pAAVLINK-U6-sgRNA-EF1alpha-5'-YE1-BE4max载体图谱质粒图谱pdf版和pAAVLINK-U6-sgRNA-EF1alpha-5'-YE1-BE4max载体序列质粒序列等相关资料下载
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