| 质粒名称: | pSF11-wElectra1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000200 |
| 产品用途: | Expresses Electra1 in C. elegans neurons |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pSF11 |
| 表达系统: | 哺乳细胞表达 |
| 启动子: | tag-168 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Electra1 |
| 物种来源: | Synthetic |
| 密码子优化: | 蠕虫表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Dual-expression system for blue fluorescent protein optimization
Papadaki S., Wang X., Wang Y., Zhang H., Jia S., Liu S., Yang M., Zhang D., Jia J., Köster R.W., Namikawa K., Piatkevich K.D.
Sci. Reports 2022 121 12, 1–16 (2022)
|
pSF11-wElectra1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSF11-wElectra1载体质粒基本信息
pSF11-wElectra1质粒图谱载体图谱和pSF11-wElectra1载体序列质粒序列多克隆位点信息
pSF11-wElectra1质粒载体简介
pSF11-wElectra1质粒序列载体序列
LOCUS pSF11-wElectra1 6859 bp DNA circular SYN 29-MAY-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6859)
AUTHORS 111
TITLE Direct Submission
JOURNAL Exported Jun 29, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6859
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 54..2717
/label=tag-168 promoter
primer_bind 2725..2747
/label=pSF11-F
misc_feature 2828..3608
/label=wElectra1-1intron
primer_bind complement(3685..3711)
/label=pSF11-R
promoter 4738..4842
/gene="bla"
/label=AmpR promoter
CDS 4843..5703
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5874..6462
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 6750..6771
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6786..6816
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6824..6840
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind join(6848..6859,1..5)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gccggatcca
61 taggtatgcc tgtcattctc tatttttaaa cgttcctgca gcagtagcac aacagagcca
121 gcagttttcc tcttcttctc catcttcttc tgcttcttcc tctcttccca aaaaaaaaaa
181 aaaaaaaaaa gtgagggggc tcactgcccc cccccccctc ccccccccct cttctccttg
241 aagctcatcc agacgtcgct gtcctcctcc tcctcgtccc cggggcgctg tgtgtgtgtg
301 tgtgtgctcc gtgagcatgt agcagcttca gcccatttaa aggcgcacac tctctgcatc
361 tctgcgtgtc tcgacactcg ttttttctct tcttttctgc tgcacacaca cacacacaca
421 cacacacgct catgccagag cacttcttgc tccggggggc tccgcgcgga gcccgagagg
481 acctcctcct cctttttctt cttcgctttt tagttcttct tgcctcggtc tcgccccttg
541 ttttgctctc catgtagtat atacatctgg gtgtgtgtgt gtgtgtgcac ctttggagca
601 ttttttgaac ttttccagaa ctttggagca acattttcag cttgttcagt gcacttttta
661 ggctgaaaat cctattttta ggttttcgaa agtttttttg gagcattttt cgtgcaattt
721 aggtgcaaaa ttaaaaaaaa gggttttgta gtgaattaaa atttttttga aactttcgcc
781 tttttaaaaa aatgtttcta atatctttaa aaattggaat attttggaaa acaatttgtt
841 ttcaattggc aatttattaa ttctgagctt ctgagcgtaa attttttttt attttggagc
901 aattctgaga tgtcttagtg catttttcgg gcatacataa aaaaaaattt atcttaataa
961 taaaaacctt aaatttaggc ttcaccgtct ggaaattcaa ttttttcagc tgaaaaactt
1021 tcaaaaaaaa aaaaattaaa ttttcacatt tattttctct ggaattttgg agcaatttca
1081 aagctgtctt agtgcatttt tagtacacaa atattttttc atttttctta aattttaata
1141 atctaagctt aaataaaggc ttcaccgtct gaaaatgcaa cttttttcag atgaaatatt
1201 aaaaaaaaaa acctctttta ttaattttgt gctccaaact ttcccacggt acgcaaatca
1261 tgtaatatgt acttttaaac attatttttc caatcaaaaa tatccaacag cttttcctta
1321 caatttgaaa atttttatta aaatttcaaa aaaatttttt ttttttttta atttcgaatt
1381 tttttaaact tgttgtaact tttttccctg tcggcgccaa ttaacaaaat acgtagaatt
1441 ttgcgcttat tgttttgtta gttaacaaat ttttgacaac tttcttatca gctgagattt
1501 aggtgtttca gttcgcaatc tgcgtctctc cttgctctcc tctattttaa aacccccata
1561 actttgttct tgtgagagtt atcaaaaaat tgtcaactaa caaaatatag agcacaaaaa
1621 tatgcacatt ttgtatgttg acaatttttt gatacctttt ttagttttcg agatatctta
1681 gttttagttt agctgtacct ttttctggca aaaaacttcc aaatttcggc aatttttcgg
1741 caaatttccc ggcaaatttc gccaagtaca tcgggctctc agacaagtgg tctgtgagtg
1801 gaagtcgata aaagtagttt acgtgctctc cgtccccccg cttcttctcc gtctctgctt
1861 ctatttctcc attgactagt cccactactt tttacggggc ggagggctct cttgcactcc
1921 agcttcttct ctttttgtgc tcttttttgc ccgtatgtgg gtgctcattt atttttcttt
1981 ggttttaaaa taaattcggt ttattaggct actcgtaaaa atgaagaaaa aacttcaaat
2041 tcaaacgaca ttaaaaaaaa atttaataaa aattttttga attgctcaaa agccccacct
2101 atatatatat atagctccgc ctacttctcg cacagacccc gcccactttt tgactccgcc
2161 cccttattgg caaaagttct tgaaaaaaat aaaaataaaa attcaaattg aaacaatttt
2221 tttttaatga agtagttttt ttctaaattg atgaaaattt ctaaacagtt tacaagcacc
2281 gcccactttt tgactccgcc cctttgttgg aaaaattctt gtaaaaaaaa ataaaaaata
2341 aaagcttctc tttcaaacga tttttagttg gtttttgtat gaaaagtttt tttcctaaaa
2401 tagaaatgtc tgaataattt gcaagctccg cccttttctt acccagactc cgccccttta
2461 ttgactaatt tttttttgtc catttacctg aagttcaaaa aaaaaattta cagtttcata
2521 gagtaataat ttttcaaaaa tctctttttc caatgtttaa tgtgtcatta taaacccgcc
2581 cagttttaag ctccgcccat ttaggccccg cccatttatg acccaaatat ttttttctct
2641 attcacccgt ggatttccct ccaacaagtt accccaaaaa taaaaataaa aattcctgca
2701 ggcgcccacc gggatccggc gcgcctctag aggatccccg ggattggcca aaggacccaa
2761 aggtatgttt cgaatgatac taacataaca tagaacattt tcaggaggac ccttggctag
2821 cgtcgacggt accaaaaatg gtttccaagg gagaggaact tatcgaagag aacatgcgca
2881 tgaaggttgt tatggaggga tctgttaacg gacatcagtt caagtgcact ggagagggag
2941 agggacgccc atacgaggga gttcaaacta tgcgcatcaa ggttatcgaa ggaggaccac
3001 ttccattcgc tttcgacatc ctcgctacct ctttcctttt cggatctaag actttcatta
3061 agtacccagc tgatatccca gacttcttca agcaatcttt cccagaagga ttcacctggg
3121 agcgcgtcac ccgctacgag gacggaggag ttgttaccgt tacccaagat acctccctcg
3181 aggacggagg acttgtttac aacgttaagg taagtttaaa catatatata ctaactaacc
3241 ctgattattt aaattttcag gttcgtggag ttaatttccc atctaacgga ccagttatgc
3301 aaaagaagac tgaaggatgg gaaccattca ctgaaatgat gtacccagct aacggaggac
3361 ttcgcggata caccgacatc gctcttaagg tcgacggaga cggacacctt cacgctaaca
3421 tcgttaccac ctaccgctct aagaagaccg ttggagatat taagatgcca ggagttcacg
3481 ctgtcgacta ccgtcttgag cgtgttgagg agtctgataa cgagacctac gttgtccttc
3541 gtgaggtcgc tgtcgctaag tactctaacc tcggaggagg aatggatgag ctttacaagt
3601 aagagctccg catcggccgc tgtcatcaga tcgccatctc gcgcccgtgc ctctgacttc
3661 taagtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta
3721 tttttgttat tatcaaaaaa acttcttctt aatttctttg ttttttagct tcttttaagt
3781 cacctctaac aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg
3841 aaaaaaattg tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt
3901 tctgtgtaca cttcttatgt tttttttact tctgataaat tttttttgaa acatcataga
3961 aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac cgcaattttt
4021 atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt
4081 ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt tcctgctttt
4141 gctttttggg ggtttcccct attgtttgtc aagagtttcg aggacggcgt ttttcttgct
4201 aaaatcacaa gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag
4261 gctcagtgga aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt
4321 ttgccttaaa tgacagaata cattcccaat ataccaaaca taactgtttc ctactagtcg
4381 gccgtacggg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
4441 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
4501 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
4561 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
4621 aaaataccgc atcaggcggc cttaattaag ggcctcgtga tacgcctatt tttataggtt
4681 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
4741 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
4801 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
4861 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
4921 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
4981 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
5041 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
5101 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
5161 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
5221 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
5281 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
5341 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
5401 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
5461 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
5521 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
5581 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
5641 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
5701 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
5761 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
5821 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
5881 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
5941 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
6001 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
6061 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
6121 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
6181 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
6241 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
6301 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
6361 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
6421 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
6481 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
6541 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
6601 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
6661 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
6721 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
6781 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
6841 tttcacacag gaaacagct
//
