| 质粒名称: | pLZ140 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002587 |
| 产品用途: | Mammalian PhCMV-driven constitutive KRAS(G12C) expression vector (PhCMV-KRAS(G12C)-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
CMV
|
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | KRAS(G12C) |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
pLZ140
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLZ140载体质粒基本信息
pLZ140质粒图谱载体图谱和pLZ140载体序列质粒序列多克隆位点信息
pLZ140质粒载体简介
pLZ140质粒序列载体序列
LOCUS pLZ140 5940 bp DNA circular SYN 28-DEC-2020
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM recombinant plasmid
REFERENCE 1 (bases 1 to 5940)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5940
/mol_type="other DNA"
/organism="recombinant plasmid"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 897..975
/label=oLZ192 Kras(G12V) F
primer_bind 897..975
/label=oLZ214 KRas(G12D) F
primer_bind 897..975
/label=oLZ215 Kras(G12A) F
primer_bind 897..975
/label=oLZ216 Kras(G12C) F
primer_bind 897..975
/label=oLZ217 Kras(G12S) F
primer_bind 897..975
/label=oLZ218 Kras(G12R) F
primer_bind 897..954
/label=oLZ188 Kras F
primer_bind 922..950
/label=oLZ190 Kras F
CDS 923..1489
/codon_start=1
/label=KRas(G12C)
/translation="MTEYKLVVVGACGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
RKHKEKMSKDGKKKKKKSKTKCVIM"
primer_bind complement(1405..1435)
/label=oLZ232 Kras(-CaaX) R
primer_bind complement(1457..1509)
/label=oLZ189 Kras R
primer_bind complement(1457..1486)
/label=oLZ191 Kras R
primer_bind complement(1487..1509)
/label=oLZ232 Kras(-CaaX) R
terminator 1537..1764
/label=bGH PA
misc_feature 2432..2509
/label=SV40 ORI
CDS 2651..3442
/label=Kan/neoR
primer_bind complement(3782..3802)
/label=M13-rev
misc_binding complement(3808..3830)
/label=LacO
promoter complement(3835..3864)
/label=lac
CDS complement(4947..5606)
/label=AmpR
promoter complement(5846..5874)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttgcca ccatgactga atataaactt gtggtagttg gagcttgtgg
961 cgtaggcaag agtgccttga cgatacagct aattcagaat cattttgtgg acgaatatga
1021 tccaacaata gaggattcct acaggaagca agtagtaatt gatggagaaa cctgtctctt
1081 ggatattctc gacacagcag gtcaagagga gtacagtgca atgagggacc agtacatgag
1141 gactggggag ggctttcttt gtgtatttgc cataaataat actaaatcat ttgaagatat
1201 tcaccattat agagaacaaa ttaaaagagt taaggactct gaagatgtac ctatggtcct
1261 agtaggaaat aaatgtgatt tgccttctag aacagtagac acaaaacagg ctcaggactt
1321 agcaagaagt tatggaattc cttttattga aacatcagca aagacaagac agggtgttga
1381 tgatgccttc tatacattag ttcgagaaat tcgaaaacat aaagaaaaga tgagcaaaga
1441 tggtaaaaag aagaaaaaga agtcaaagac aaagtgtgta attatgtaag cggccgctcg
1501 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
1561 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
1621 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
1681 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
1741 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
1801 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
1861 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
1921 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
1981 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
2041 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
2101 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
2161 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
2221 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
2281 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
2341 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
2401 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
2461 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
2521 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
2581 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
2641 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
2701 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
2761 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
2821 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
2881 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
2941 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
3001 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
3061 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
3121 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
3181 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
3241 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
3301 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
3361 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
3421 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
3481 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
3541 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
3601 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
3661 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
3721 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
3781 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
3841 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
3901 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
3961 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
4021 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
4081 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
4141 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
4201 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
4261 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
4321 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
4381 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
4441 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
4501 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
4561 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
4621 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
4681 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag
4741 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
4801 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
4861 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
4921 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
4981 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
5041 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
5101 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
5161 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
5221 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
5281 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
5341 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
5401 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
5461 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
5521 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
5581 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
5641 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
5701 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
5761 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
5821 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
5881 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
pLZ140载体图谱质粒图谱pdf版和pLZ140载体序列质粒序列等相关资料下载
pLZ140质粒载体应用举例
- 上一篇：pLZ141
- 下一篇：没有了
