| 质粒名称: | pAAVLINK-EFS-HA-5'-BTAF1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001868 |
| 产品用途: | 5' AAVLINK plasmid for BTAF1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EFS |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | BTAF1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EFS-HA-5'-BTAF1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EFS-HA-5'-BTAF1载体质粒基本信息
pAAVLINK-EFS-HA-5'-BTAF1质粒图谱载体图谱和pAAVLINK-EFS-HA-5'-BTAF1载体序列质粒序列多克隆位点信息
pAAVLINK-EFS-HA-5'-BTAF1质粒载体简介
pAAVLINK-EFS-HA-5'-BTAF1质粒序列载体序列
LOCUS pAAVLINK-EFS-HA-5'-BTAF1 7364 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7364)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7364
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 145..356
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
CDS 372..398
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 405..4504
/codon_start=1
/gene="BTAF1"
/gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
/product="TATA-binding protein-associated factor 172"
/label=BTAF1
/note="TBP-associated factor 172; B-TFIID transcription
factor-associated 170 kDa subunit; ATP-dependent helicase
BTAF1; BTAF1 RNA polymerase II, B-TFIID transcription
factor-associated, 170kDa (Mot1 homolog, S. cerevisiae)"
/db_xref="GI:27477070"
/db_xref="CCDS:CCDS7419.1"
/db_xref="GeneID:9044"
/db_xref="HGNC:HGNC:17307"
/db_xref="MIM:605191"
/protein_id="NP_003963.1"
/translation="AVSRLDRLFILLDTGTTPVTRKAAAQQLGEVVKLHPHELNNLLSK
VLIYLRSANWDTRIAAGQAVEAIVKNVPEWNPVPRTRQEPTSESSMEDSPTTERLNFDR
FDICRLLQHGASLLGSAGAEFEVQDEKSGEVDPKERIARQRKLLQKKLGLNMGEAIGMS
TEELFNDEDLDYTPTSASFVNKQPTLQAAELIDSEFRAGMSNRQKNKAKRMAKLFAKQR
SRDAVETNEKSNDSTDGEPEEKRRKIANVVINQSANDSKVLIDNIPDSSSLIEETNEWP
LESFCEELCNDLFNPSWEVRHGAGTGLREILKAHGKSGGKMGDSTLEEMIQQHQEWLED
LVIRLLCVFALDRFGDFVSDEVVAPVRETCAQTLGVVLKHMNETGVHKTVDVLLKLLTQ
EQWEVRHGGLLGIKYALAVRQDVINTLLPKVLTRIIEGLQDLDDDVRAVAAASLVPVVE
SLVYLQTQKVPFIINTLWDALLELDDLTASTNSIMTLLSSLLTYPQVQQCSIQQSLTVL
VPRVWPFLHHTISSVRRAALETLFTLLSTQDQNSSSWLIPILPDMLRHIFQFCVLESSQ
EILDLIHKVWMELLSKASVQYVVAAACPWMGAWLCLMMQPSHLPIDLNMLLEVKARAKE
KTGGKVRQGQSQNKEVLQEYIAGADTIMEDPATRDFVVMRARMMAAKLLGALCCCICDP
GVNVVTQEIKPAESLGQLLLFHLNSKSALQRISVALVICEWAALQKECKAVTLAVQPRL
LDILSEHLYYDEIAVPFTRMQNECKQLISSLADVHIEVGNRVNNNVLTIDQASDLVTTV
FNEATSSFDLNPQVLQQLDSKRQQVQMTVTETNQEWQVLQLRVHTFAACAVVSLQQLPE
KLNPIIKPLMETIKKEENTLVQNYAAQCIAKLLQQCTTRTPCPNSKIIKNLCSSLCVDP
YLTPCVTCPVPTQSGQENSKGSTSEKDGMHHTVTKHRGIITLYRHQKAAFAITSRRGPT
PKAVKAQIADLPAGSSGNILVELDEAQKPYLVQRRGAEFALTTIVKHFGGEMAVKLPHL
WDAMVGPLRNTIDINNFDGKSLLDKGDSPAQELVNSLQVFETAAASMDSELHPLLVQHL
PHLYMCLQYPSTAVRHMAARCVGVMSKIATMETMNIFLEKVLPWLGAIDDSVKQEGAIE
ALACVMEQLDVGIVPYIVLLVVPVLGRMSDQTDSVRFMATQCFATLIRLMPLEAGIPDP
PNMSAELIQLKAKERHFLEQLLDGKKLENYKIPVPINAELRKYQQDGVNWLAFLNKYKL
HGILCDDMGLGKTLQSICILAGDHCHRAQEYARSKLAECMPLPSLVVCPPTLTGHWVDE
VGKFCSREYLNPLHYTGPPTERI"
misc_feature 1291..1296
/label=SDS
misc_feature 3611..3616
/label=SDS
intron 4505..4545
/label=Splicing Donor
misc_feature 4549..4582
/label=lox2272-JT1571
repeat_region 4627..4767
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4627..4756
/label=AAV2 ITR
repeat_region 4628..4768
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4842..5297
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5579..5683
/gene="bla"
/label=AmpR promoter
CDS 5684..6544
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6715..7303
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgggcag agcgcacatc gcccacagtc cccgagaagt
181 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg
241 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
301 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagcgta
361 cggccaccat gtacccttat gacgtacctg actatgctgg atctgcggtc tccaggctag
421 atcgcctttt tattttactg gatactggca ctactcctgt tacaagaaaa gctgctgcac
481 agcaacttgg agaagtggtg aagcttcatc cccatgaact aaataatctc ctgtctaaag
541 tgttgatata tttaaggagt gcaaattggg atacccggat tgcagcagga caagctgttg
601 aagctatagt gaaaaatgta cctgagtgga atccagtgcc gagaaccaga caagaaccta
661 cttccgaaag ttctatggaa gattcaccta ctacagagcg attgaatttt gacagatttg
721 atatatgtag attgttacaa catggtgcat cactcctggg atctgctggt gccgaatttg
781 aagtccaaga tgaaaaatca ggtgaagtgg atcctaaaga gaggatagca cgccaacgaa
841 aattattaca gaagaaactt ggccttaata tgggagaagc aattggaatg agtactgaag
901 aacttttcaa tgatgaggat ttggattata ccccaacttc agcatccttt gttaacaaac
961 aacctactct tcaggcagct gaattgattg actcagagtt tcgagcagga atgagcaata
1021 gacaaaagaa caaagctaaa agaatggcca agttatttgc aaaacagaga tccagggatg
1081 cagtggaaac taatgagaag agcaatgata gcactgatgg ggagccagaa gaaaagagac
1141 ggaaaatagc aaatgttgtt attaatcagt ctgcaaatga ttccaaagtc ttgattgata
1201 atattccaga cagctcttcc ttaattgaag agacaaatga atggcctttg gaaagctttt
1261 gtgaagaact ttgcaatgac ctttttaatc cctcctggga ggttcgacat ggtgcaggca
1321 ctggacttag ggaaattctt aaagctcatg ggaaaagtgg tggtaaaatg ggtgacagca
1381 ctttagaaga gatgattcag cagcatcaag agtggttgga agacttggtt attagactcc
1441 tttgtgtttt tgcattagac agatttggag actttgtttc tgatgaagtt gtggcaccag
1501 ttcgtgaaac ttgtgctcaa acattaggtg tggttttaaa acacatgaac gaaacaggag
1561 ttcataagac tgtggatgtg ctgctaaaat tacttacaca agaacaatgg gaagttagac
1621 atggtggtct gctgggaata aaatatgctt tggcagtccg tcaggatgta attaatactt
1681 tattgcctaa agttttaact agaataattg aaggactcca ggatcttgat gatgatgtca
1741 gagctgttgc tgcagcatca ttagtgcctg tagtagaaag ccttgtctat cttcagacac
1801 aaaaggtgcc cttcattata aatacattgt gggatgctct tctggaatta gatgatctaa
1861 cagcttcaac aaatagtatt atgactctcc tttcatcctt gttaacttac cctcaggtcc
1921 aacaatgcag tattcagcag tcactgacag ttttagttcc acgtgtctgg ccttttttgc
1981 atcacactat atcatcagtt cgaagagcag cattggaaac tctgtttacg ttattatcaa
2041 cacaggacca gaactcttca tcttggctta tacctatact gcctgatatg ctccgacaca
2101 tttttcagtt ctgtgttttg gaaagcagcc aggaaattct ggaccttatt cacaaggttt
2161 ggatggaact gttgagtaag gcttcagttc agtatgtggt agcagctgct tgcccatgga
2221 tgggtgcttg gctttgcttg atgatgcagc cttctcattt acctatcgat ttaaatatgt
2281 tgctagaagt aaaagctaga gccaaggaaa aaacaggtgg taaggtgcgc caaggccaaa
2341 gccagaataa agaagtactt caggagtata ttgcaggtgc cgacaccatc atggaagacc
2401 cagccaccag ggattttgta gttatgcggg ccagaatgat ggcagccaag ttgttaggag
2461 cactttgttg ctgtatttgt gatccaggtg taaatgtggt aactcaagaa attaaaccag
2521 ctgaatccct gggccagtta ctactcttcc atttgaactc caagtctgct ttacagagga
2581 ttagtgtagc tttggtaatc tgtgaatggg ctgctttaca aaaggagtgt aaagctgtta
2641 ccttagctgt gcagccgcgt ttacttgata tcctttcaga acatttatat tatgacgaaa
2701 ttgccgttcc attcacacga atgcagaatg aatgtaaaca acttatatca tcattagctg
2761 atgtacatat tgaagttggt aatcgagtaa acaacaatgt tttaacaata gatcaagcta
2821 gtgacttggt cactactgtt tttaatgaag ccacatcatc tttcgattta aatcctcaag
2881 tgttacaaca gttagatagt aaacgacagc aggtccaaat gacagttaca gagaccaacc
2941 aggagtggca agtgttgcag ttgagagtgc atacttttgc tgcctgtgca gttgtgagct
3001 tgcagcagct tccggagaaa ttaaatccta tcataaaacc attaatggag acaattaaaa
3061 aagaagagaa tacactagtg caaaactatg cagctcagtg catagctaaa ctccttcagc
3121 agtgcacaac aaggacgccc tgtcccaatt caaaaattat taaaaacctc tgtagctcac
3181 tttgtgtgga cccatatcta actccttgtg tcacatgtcc agtaccaaca caaagtggcc
3241 aggaaaattc taaaggatcc acctcagaaa aagatggaat gcaccatact gtcaccaagc
3301 acagaggtat aattacactc tacaggcacc agaaagctgc ctttgctatc acaagtaggc
3361 gaggtcctac ccccaaagca gtaaaagctc aaatagcaga tcttcctgca ggaagtagtg
3421 gaaatattct tgttgaactt gatgaggccc agaagcctta cctggtacaa cggagaggag
3481 ctgaatttgc tttgacaact atagtaaagc attttggtgg tgaaatggca gtgaagttgc
3541 cacatctctg ggatgctatg gttggcccat tgaggaatac aatcgacata aataattttg
3601 atggaaaatc cctcctggat aagggagata gccctgctca agaattggtg aattctctgc
3661 aggtttttga aacagcagca gcttcaatgg attctgagct tcatcccttg ttggtccagc
3721 atttgccaca tctttatatg tgccttcaat accccagtac tgcagtgagg cacatggctg
3781 ctcgttgtgt aggtgtcatg agcaaaatag ctaccatgga aacaatgaat atttttttgg
3841 agaaggttct tccgtggctg ggagcaattg atgacagtgt caaacaagag ggtgcaattg
3901 aagcacttgc ctgtgtaatg gaacaactag atgttggtat tgttccatat attgtccttt
3961 tagttgttcc tgtattagga agaatgagtg atcagacaga cagtgtgaga ttcatggcca
4021 cgcagtgctt tgcaacgcta attagactca tgccacttga ggcaggcatt ccagaccctc
4081 caaacatgtc agcagaatta atccaattga aagccaagga gcgacacttt ttggagcaat
4141 tgttagatgg gaaaaaattg gaaaattata aaattccagt accaatcaat gctgaactca
4201 gaaaatatca gcaggatggt gtgaactggt tagcatttct taataagtat aaacttcatg
4261 gaattctgtg tgatgacatg ggtttaggaa aaactttaca gtccatctgc attctagcag
4321 gagatcattg tcatagggcc caggaatatg caagatcaaa attagcagaa tgtatgccac
4381 ttccttcctt agtggtttgt ccgccaacat taacaggcca ttgggtggat gaagtaggta
4441 aattttgctc tagagaatat ctcaacccgt tgcattacac tggacctccc actgaaagaa
4501 taaggtaagt atcaaggtta caagacaggt ttaaggagac caataagcta ttattcgtat
4561 aggatacttt atacgaagtt attaagttat ggagaccggt gtggcacgtg cggaccgagc
4621 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
4681 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
4741 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
4801 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
4861 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt
4921 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
4981 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
5041 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
5101 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
5161 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
5221 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
5281 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
5341 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
5401 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
5461 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
5521 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
5581 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
5641 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
5701 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
5761 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
5821 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
5881 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
5941 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
6001 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
6061 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
6121 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
6181 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
6241 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
6301 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
6361 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
6421 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
6481 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
6541 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
6601 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
6661 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
6721 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
6781 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
6841 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
6901 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
6961 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
7021 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
7081 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
7141 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
7201 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
7261 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
7321 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
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