| 质粒名称: | pREDawn-AmpR-MCS |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 188978 |
| 存储实验室: | Heikki Takala |
|
载体骨架基本信息 |
|
| 载体名称: | pUC |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 9077bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 克隆方法: | 限制性内切酶 |
| 3’测序引物 | T7term |
|
文献引用方法 |
|
| 材料和方法部分: | pREDawn-AmpR-MCS was a gift from Heikki Takala (Addgene plasmid # 188978 ; http://n2t.net/addgene:188978 ; RRID:Addgene_188978) |
| 参考文献部分: | Optogenetic Control of Bacterial Expression by Red Light. Multamaki E, Garcia de Fuentes A, Sieryi O, Bykov A, Gerken U, Ranzani AT, Kohler J, Meglinski I, Moglich A, Takala H. ACS Synth Biol. 2022 Aug 23. doi: 10.1021/acssynbio.2c00259. 10.1021/acssynbio.2c00259 PubMed 35998606 |
买家导航
pREDawn-AmpR-MCS载体质粒基本信息
pREDawn-AmpR-MCS质粒图谱载体图谱和pREDawn-AmpR-MCS载体序列质粒序列多克隆位点信息
pREDawn-AmpR-MCS质粒载体简介
pREDawn-AmpR-MCS质粒序列载体序列
LOCUS pREDawn-AmpR-MCS 9077 bp DNA circular SYN 14-APR-2025
DEFINITION For red light-induced bacterial expression of gene of interest
(AmpR).
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9077)
AUTHORS Multamaki E, Garcia de Fuentes A, Sieryi O, Bykov A, Gerken U,
Ranzani AT, Kohler J, Meglinski I, Moglich A, Takala H
TITLE Optogenetic Control of Bacterial Expression by Red Light.
JOURNAL ACS Synth Biol. 2022 Aug 23. doi: 10.1021/acssynbio.2c00259.
PUBMED 35998606
REFERENCE 2 (bases 1 to 9077)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
Biol. 2022 Aug 23. doi: 10.1021/acssynbio.2c00259."
COMMENT Sequence Label: pREDawn-AmpR-MCS
FEATURES Location/Qualifiers
source 1..9077
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator complement(26..73)
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
primer_bind 69..87
/label=T7 Term
/note="T7 terminator, reverse primer"
CDS complement(140..157)
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS complement(205..237)
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/label=T7 tag (gene 10 leader)
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS complement(241..258)
/codon_start=1
/product="thrombin recognition and cleavage site"
/label=thrombin site
/translation="LVPRGS"
CDS complement(268..285)
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
RBS 304..326
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
terminator complement(402..429)
/label=T7Te terminator
/note="phage T7 early transcription terminator"
terminator complement(445..516)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(564..596)
/codon_start=1
/product="C-terminal peptide that mediates degradation in
bacteria through the ClpXP and ClpAP proteases (McGinness
et al., 2006)"
/label=ssrA tag (LVA)
/note="mutant LVA variant that confers accelerated
degradation under some conditions (Andersen et al., 1998)"
/translation="AANDENYALVA"
CDS complement(597..1307)
/codon_start=1
/gene="cI"
/product="phage lambda repressor"
/label=lambda repressor
/translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
VGKVIASQWPEETFG"
RBS 1314..1325
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
primer_bind complement(1648..1667)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
promoter 1873..1950
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
CDS 6226..6417
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
primer_bind complement(6411..6433)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 6519..6658
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 6574..6593
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(6673..6690)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6844..7432)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6924..6943)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
promoter 7554..7658
/gene="bla"
/label=AmpR promoter
CDS join(7659..7727,7728..8519)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7877..7896)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin complement(8611..9066)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8748..8769)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 8960..8979
/label=F1ori-R
/note="F1 origin, reverse primer"
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatatg
241 gctgccgcgc ggcaccaggc cgctgctgtg atgatgatga tgatggctgc tgcccatggt
301 atatctcctt cttaaagtta aacaaaatta tttctagagc aaccattatc accgccagag
361 gtaaaatagt caacacgcac ggtgttactc tagtatataa acgcagaaag gcccacccga
421 aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga taaaacgaaa
481 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggctctag tagcgatcta
541 cactagcact atcagcgtta ttaagctact aaagcgtagt tttcgtcgtt tgcagcgcca
601 aacgtctctt caggccactg actagcgata actttcccca caacggaaca actctcattg
661 catgggatca ttgggtactg tgggtttagt ggttgtaaaa acacctgacc gctatccctg
721 atcagtttct tgaaggtaaa ctcatcaccc ccaagtctgg ctatgcagaa atcacctggc
781 tcaacagcct gctcagggtc aacgagaatt aacattccgt caggaaagct tggcttggag
841 cctgttggtg cggtcatgga attaccttca acctcaagcc agaatgcaga atcactggct
901 tttttggttg tgcttaccca tctctccgca tcacctttgg taaaggttct aagctcaggt
961 gagaacatcc ctgcctgaac atgagaaaaa acagggtact catactcact tctaagtgac
1021 ggctgcatac taaccgcttc atacatctcg tagatttctc tggcgattga agggctaaat
1081 tcttcaacgc taactttgag aatttttgca agcaatgcgg cgttataagc atttaatgca
1141 ttgatgccat taaataaagc accaacgcct gactgcccca tccccatctt gtctgcgaca
1201 gattcctggg ataagccaag ttcatttttc tttttttcat aaattgcttt aaggcgacgt
1261 gcgtcctcaa gctgctcttg tgttaatggt ttcttttttg tgctcatcta gtatttctcc
1321 tcttttctag actccgttgt gatgacgcat tggtacgcgg tatcgggagg ttcgaaaatt
1381 tcgagcgata tcttaagggg ggtgccttac gtagaacccc gtaggtcatg cccgaggccg
1441 gtcctggatg gcgcggcgga tacgcttgag caggttttcg tcgagaagcg gcttcaaaac
1501 cacgtctttt acgccggcct cggcggcccg ggtcgagatg ttttcgtccg gatagccggt
1561 gatcaggatc acgggcgtag atctcgatcc tctacgccgg acgcatcgtg gccggcatca
1621 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
1681 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
1741 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc
1801 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
1861 gtcgagatcc cggacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc
1921 ccggaagaga gtcaattgag ggtggtgaat gtgctccgac tcatgatcat gaagctttta
1981 caaagggaga ccctggacct gcaccgcgag gtcgaggccc tgatgcccgt gatgagtgag
2041 acgctgaccc ggcggggcta tctggaggtg ctgcggcgcc tgcaagcggt ggtcgcgccg
2101 ctggaagcac aactcgacgc cctgccgctg ccggacgagc tggcgtggcc cgagcgccgc
2161 aaggcccatc tgctcgctct ggaccttgcc gtaatggggg aagggccgtc aaatcagcag
2221 gcgccactgt ctgtgcccga gctgaccact ctggcgcagg ccttcggagc gctttacgtg
2281 cttgaaggcg cgacgctagg cggacagctc atcagccgcc atctcaggcg cacgctgggg
2341 ctaagccctg agcagggcag cgcctatttc tccggctatg gcccccagac cggcccgcgc
2401 tggcgctcgt tcggggaagt gctggaagcc agcgtgccgg cagaagacgc ggcagaggtc
2461 gtggcgggag cgcggcagac cttcggggcc ttccgccgcg ccttgcaggg gctgtcggaa
2521 gctgaagcgg tccaagtgcc tgaggtcgcc cacgcatgag ccgggacccg ttgccctttt
2581 ttccaccgct ttaccttggt ggcccggaaa ttaccaccga gaactgcgag cgcgagccga
2641 ttcatattcc cggcagcatc cagccgcacg gcgccctgct cactgccgac gggcacagcg
2701 gcgaggtgct ccagatgagc ctcaacgcgg ccacttttct gggacaggaa cccacagtgc
2761 tgcgcggaca gaccctcgcc gcactgctgc ccgagcagtg gcccgcgctg caagcggccc
2821 tgccccccgg ctgccccgac gccctgcaat accgcgcaac gctggactgg cctgccgccg
2881 ggcacctttc gctgacggtg caccgggtcg gcgagttgct gattctggaa ttcgagccga
2941 cggaggcctg ggacagcacc gggccgcacg cgctgcgcaa cgcgatgttc gcgctcgaaa
3001 gtgcccccaa cctgcgggcg ctggccgagg tggcgaccca gacggtccgc gagctgacgg
3061 gctttgaccg ggtgatgctc tacaaatttg cccccgacgc caccggcgaa gtgattgccg
3121 aggcccgccg tgaggggctg cacgcctttc tgggccaccg ttttcccgcg tcggacattc
3181 cggcgcaggc ccgcgcgctc tacacccggc acctgctgcg cctgaccgcc gacacccgcg
3241 ccgccgccgt gccgctcgat cccgtcctca acccgcagac gaatgcgccc accccgctgg
3301 gcggcgccgt gctgcgcgcc acctcgccca tgcacatgca gtacctgcgg aacatgggcg
3361 tcgggtcgag cctgtcggtg tcggtggtgg tcggcggcca gctctggggc ctgatcgcct
3421 gccaccacca gacgccctac gtgttgccgc ccgacctgcg aaccacgctc gaatacctgg
3481 gccgcttgct gagcctgcaa gttcaggtca aggaagcggc ggacgtggcg gcctttcgcc
3541 agagcctgcg ggagcaccac gcgcgggtgg ccctcgcggc ggcgcactcg ctctcgccgc
3601 acgacaccct cagtgacccg gcgcttgacc tgctgggcct gatgcgggcc gggggcctga
3661 ttctgcgttt cgagggccgc tggcagacgt tgggtgaagt gccgcctgcc ccggcggtgg
3721 acgcgctgct ggcgtggctc gaaacccagc cgggcgccct ggtccagacc gacgcgctag
3781 gccaactgtg gcccgccggc gccgatctcg cccccagcgc agcgggcctg ctcgccatca
3841 gcgtgggcga gggctggtcg gagtgcctcg tctggctgcg gcccgaactg cggctggagg
3901 tcgcctgggg cggggccact cctgaccagg cgaaagacga cctcgggccg cgccactcat
3961 tcgacaccta cctcgaagaa aaacgcggct acgccgagcc ctggcatccc ggcgaaatcg
4021 aggaggcgca ggatctacgt gacacattga ccggggcgct gggcgagcgc ctgctccagg
4081 aactgcaatc cgagctcgtc cacgtctcca ggctgagcgc catgggcgaa atggcgtccg
4141 cgctcgcgca cgagctcaac cagccgctgg cggcgatcag caactacatg aagggctcgc
4201 ggcggctgct tgccggcagc agtgatccga acacaccgaa ggtcgaaagc gccctggacc
4261 gcgccgccga gcaggcgctg cgcgccggcc agatcatccg gcgcctgcgc gacttcgttg
4321 cccgcggcga atcggagaag cgggtcgaga gtctctccaa gctgatcgag gaggccggcg
4381 cgctcgggct tgccggcgcg cgcgagcaga acgtgcagct ccgcttcagt ctcgatccgg
4441 gcgccgatct cgttctcgcc gaccgggtgc agatccagca ggtcctggtc aacctgttcc
4501 gcaacgcgct ggaagcgatg gctcagtcgc agcgacgcga gctcgtcgtc accaacaccc
4561 ccgccgccga cgacatgatc gaggtcgaag tgtccgacac cggcagcggt ttccaggacg
4621 acgtcattcc gaacctgttt cagactttct tcaccaccaa ggacaccggc atgggcgtgg
4681 gactgtccat cagccgctcg atcatcgaag ctcacggcgg gcgcatgtgg gccgagagca
4741 acgcatcggg cggggcgacc ttccgcttca ccctcccggc agccgacgag atgataggag
4801 gtctagcatg acgaccaagg gacatatcta cgtcatcgac gacgacgcgg cgatgcggga
4861 ttcgctgaat ttcctgctgg attctgccgg cttcggcgtc acgctgtttg acgacgcgca
4921 agcctttctc gacgccctgc cgggtctctc cttcggctgc gtcgtctccg acgtgcgcat
4981 gccgggcctt gacggcatcg agctgttgaa gcggatgaag gcgcagcaaa gcccctttcc
5041 gatcctcatc atgaccggtc acggcgacgt gccgctcgcg gtcgaggcga tgaagttagg
5101 ggcggtcgac tttctggaaa agcctttcga ggacgaccgc ctcaccgcca tgatcgaatc
5161 ggcgatccgc caggccgagc cggccgccaa gagcgaggcc gtcgcgcagg atatcgccgc
5221 ccgcgtcgcc tcgttgagcc ccagggagcg ccaggtcatg gaagggctga tcgccggcct
5281 ttccaacaag ctgatcgccc gcgagtacga catcagcccg cgcaccatcg aggtgtatcg
5341 ggccaacgtc atgaccaaga tgcaggccaa cagcctttcg gagctggttc gcctcgcgat
5401 gcgcgccggc atgctcaacg attgacaatt gatgtaagtt agctcactca ttaggcaccg
5461 ggatctcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
5521 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
5581 gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg
5641 atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact
5701 ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggcccca
5761 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt
5821 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa
5881 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg
5941 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
6001 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
6061 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
6121 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
6181 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa
6241 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
6301 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
6361 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
6421 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
6481 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
6541 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
6601 tgcaccattg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
6661 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
6721 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
6781 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
6841 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
6901 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
6961 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
7021 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
7081 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
7141 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
7201 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
7261 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
7321 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
7381 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
7441 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
7501 ggtcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttcgcggaa
7561 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
7621 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
7681 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
7741 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
7801 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
7861 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
7921 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
7981 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
8041 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
8101 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
8161 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt
8221 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
8281 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
8341 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
8401 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
8461 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaag
8521 aattaattca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
8581 ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat
8641 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
8701 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
8761 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
8821 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
8881 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
8941 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
9001 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
9061 gcgcgtccca ttcgcca
//
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pREDawn-AmpR-MCS质粒载体应用举例
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