| 质粒名称: | pYK650 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000832 |
| 产品用途: | Mammalian PhCMV-driven constitutive GNCR-ZFP1-3xFLAG-NLS expression vector (PhCMV-GNCR-ZFP1-3xFLAG-NLS-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | GNCR-ZFP1-3xFLAG-NLS |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK650
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK650载体质粒基本信息
pYK650质粒图谱载体图谱和pYK650载体序列质粒序列多克隆位点信息
pYK650质粒载体简介
pYK650质粒序列载体序列
LOCUS pYK650 6837 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6837)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6837
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 880..946
/label=oYF0019
primer_bind 881..918
/label=oYK421_GNCR_F
primer_bind 881..902
/label=oYK31-mCherry-NS3a-F
primer_bind 881..899
/label=oYK06-ANR-mEGFP-F
primer_bind 881..899
/label=oYK1125_ZF1_F
primer_bind 910..952
/label=oYF005
primer_bind 916..937
/label=oYK481_GNCR_F
primer_bind 916..937
/label=oYK483_LCK1_F
primer_bind 916..936
/label=oYK490_P2A_GNCR1_F
CDS 919..1617
/codon_start=1
/label=GNCR1-N
/translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
KAEEVKERCKS"
primer_bind 919..952
/label=oYF0011
primer_bind 919..943
/label=oLZ146 GNCR1-N F
primer_bind 919..943
/label=oSCL762
primer_bind 1585..1597
/label=oYK03-02-F
primer_bind 1585..1596
/label=oYK31-mCherry-NS3a-F
primer_bind complement(1596..1653)
/label=oYF0020
primer_bind complement(1596..1647)
/label=oYF006
primer_bind complement(1596..1618)
/label=oYF0012
primer_bind complement(1605..1624)
/label=oYK465_GNCR1_R
primer_bind 1615..1637
/label=oSCL1129
primer_bind 1615..1637
/label=oSCL1192
primer_bind 1615..1635
/label=oLZ147 NS3a F
primer_bind 1615..1634
/label=oYF0033
primer_bind 1617..1637
/label=oLZ048 NSP3-F
primer_bind 1617..1634
/label=oYK419_GS_F
primer_bind 1618..1647
/label=oSCL388
primer_bind 1618..1633
/label=oYK28-mCherry-NS3a-F
primer_bind complement(1619..1667)
/label=oLZ145 DNCR2-GNCR1 R
primer_bind complement(1622..1637)
/label=oYK520_GS_R
primer_bind complement(1625..1643)
/label=Oscl18-4
primer_bind complement(1628..1648)
/label=oSCL1119
primer_bind complement(1628..1647)
/label=oSCL075
primer_bind complement(1628..1647)
/label=oSCL075a
primer_bind complement(1628..1647)
/label=oSCL075`b
primer_bind complement(1628..1647)
/label=oSCL075`c
primer_bind complement(1631..1647)
/label=oYK472_NLS_R
primer_bind 1632..1652
/label=oLZ146 GNCR1-N F
primer_bind complement(1632..1648)
/label=oYK92_L7Ae_R
primer_bind complement(1634..1662)
/label=oYK489_GNCR1_R
misc_feature 1634..1653
/label=linker
primer_bind complement(1636..1648)
/label=oYK75_NLS_mcp_R
primer_bind complement(1645..1668)
/label=oYK421_GNCR3_R
primer_bind 1649..1686
/label=oYK650_ZF_F
misc_feature 1654..1668
/label=NSP3
primer_bind 1668..1689
/label=oYK1125_ZF1_F
CDS 1669..2196
/codon_start=1
/label=ZF1
/translation="SRPGERPFQCRICMRNFSEEANLRRHTRTHTGEKPFQCRICMRNF
SDHSSLKRHLRTHTGSQKPFQCRICMRNFSQSANLLRHTRTHTGEKPFQCRICMRNFSD
PSSLKRHLRTHTGSQKPFQCRICMRNFSQQTNLTRHTRTHTGEKPFQCRICMRNFSDAT
QLVRHLRTHLRGS"
primer_bind 1669..1696
/label=oYK1126_ZF1_F
primer_bind 2201..2224
/label=oYK563_Flag_F
CDS 2206..2271
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
primer_bind complement(2246..2309)
/label=oYK502_ZF_R
CDS 2278..2298
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind 2300..2321
/label=oSCL1129
primer_bind 2300..2321
/label=oSCL1192
primer_bind 2300..2319
/label=oLZ147 NS3a F
primer_bind 2300..2318
/label=oYF0033
misc_feature 2302..2331
/label=linker
primer_bind 2302..2331
/label=oSCL388
misc_feature 2302..2322
/label=linker
primer_bind 2302..2321
/label=oLZ048 NSP3-F
primer_bind 2302..2318
/label=oYK419_GS_F
primer_bind 2302..2317
/label=oYK28-mCherry-NS3a-F
primer_bind complement(2303..2331)
/label=oLZ145 DNCR2-GNCR1 R
primer_bind complement(2306..2321)
/label=oYK520_GS_R
primer_bind complement(2309..2327)
/label=Oscl18-4
primer_bind complement(2312..2331)
/label=oSCL075
primer_bind complement(2312..2331)
/label=oSCL075a
primer_bind complement(2312..2331)
/label=oSCL075`b
primer_bind complement(2312..2331)
/label=oSCL1119
primer_bind complement(2312..2331)
/label=oSCL075`c
primer_bind complement(2315..2357)
/label=oYK472_NLS_R
primer_bind 2316..2331
/label=oLZ146 GNCR1-N F
primer_bind complement(2316..2331)
/label=oYK92_L7Ae_R
primer_bind complement(2318..2331)
/label=oYK489_GNCR1_R
primer_bind complement(2320..2331)
/label=oYK75_NLS_mcp_R
misc_feature 2327
/label=linker
primer_bind complement(2335..2357)
/label=oYK57-MCP-mCherry-R
terminator 2431..2658
/label=bGH PA
misc_feature 2704..3132
/label=f1 origin
misc_feature 3137..3480
/label=SV40 early promoter and origin
misc_feature 3326..3403
/label=SV40 ORI
CDS 3545..4336
/label=Kan/neoR
misc_feature 4510..4640
/label=SV40 early polyadenylation signal
primer_bind complement(4676..4696)
/label=M13-rev
misc_binding complement(4702..4724)
/label=LacO
promoter complement(4729..4758)
/label=lac
misc_feature complement(5023..5696)
/label=pUC origin
CDS complement(5844..6503)
/label=AmpR
promoter complement(6743..6771)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gctggctagg ccaccatgga catcgagaag ctgtgcaaga aggcggaaga ggaggccaag
961 gaggcccagg agaaggccga cgagctgaga caacgacacc ccgattcgca ggcggcggaa
1021 gacgcggagg atctcgcaaa tctggctgtc gccgccgtgc tgaccgcttg tctgctggcc
1081 caggagcatc ccaacgccga tatcgctaag ctgtgcatca aggccgcgag cgaagctgcg
1141 gaggccgcgt caaaagccgc cgagctggcc caacggcacc cagacagtca agcagccaga
1201 gatgctatca aactcgcctc tcaagccgcc cgcgccgtga tcctggccat catgctcgcc
1261 gcagagaatc ccaacgctga tattgcaaag ctatgtatta aggcggccag cgaagccgca
1321 gaggctgcct cgaaagctgc ggaacttgca cagcggcacc ccgactccca ggccgcccga
1381 gatgcaatta aactggccag tcaggccgct gaggcggtgg agagagccat ctggctggcc
1441 gccgagaacc ccaatgccga tatcgccaaa aaatgcatca aagcggcctc cgaagcagcc
1501 gaggaggcct ctaaagctgc cgaagaggct caaagacacc cagacagcca gaaggcacgc
1561 gacgagatca aggaggcctc tcagaaggcc gaggaggtga aggagagatg caagagcgcc
1621 agcggaagtg gcggaggagg cgacgtcgct agcatgctca agatggagtc taggcctgga
1681 gagaggcctt tccagtgcag gatctgcatg aggaactttt ctgaggaggc caacctgagg
1741 aggcacacca gaacccacac cggagagaag ccctttcagt gcaggatttg catgaggaat
1801 ttctccgacc acagcagcct gaagagacac ctgagaaccc acacaggaag ccagaagccc
1861 ttccagtgca gaatctgcat gagaaacttc agccagagcg ccaacctgct gagacacacc
1921 agaacacaca ccggagaaaa gcccttccag tgtagaatct gcatgcggaa cttctccgac
1981 cccagcagcc tgaaaagaca cctgaggacc cacacaggca gccagaagcc attccagtgc
2041 cgcatctgca tgcgcaactt cagccagcag accaacctga ccagacacac ccgcacccac
2101 accggcgaaa agcctttcca gtgtcgcatc tgcatgagaa atttcagcga cgccacccag
2161 ctggtgagac acctgcggac acacctgaga ggcagcacct gcagagacta caaagaccat
2221 gacggcgact ataaagatca cgacatcgat tacaaagacg acgacgacaa gatggctcca
2281 aagaagaaga gaaaggtggg tgccagcgga agtggcggag gaggcgacgt ctaaggatcc
2341 actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta
2401 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
2461 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
2521 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
2581 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
2641 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
2701 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
2761 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
2821 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
2881 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
2941 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
3001 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
3061 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
3121 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
3181 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
3241 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
3301 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
3361 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
3421 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
3481 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
3541 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
3601 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
3661 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
3721 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
3781 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
3841 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
3901 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
3961 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
4021 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
4081 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
4141 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
4201 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
4261 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
4321 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
4381 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
4441 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
4501 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
4561 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
4621 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
4681 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
4741 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
4801 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
4861 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
4921 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
4981 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
5041 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
5101 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
5161 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
5221 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
5281 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
5341 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
5401 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
5461 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
5521 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
5581 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
5641 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
5701 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
5761 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
5821 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
5881 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
5941 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
6001 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
6061 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
6121 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
6181 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
6241 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
6301 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
6361 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
6421 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
6481 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
6541 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
6601 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
6661 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
6721 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
6781 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
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