pCDH-CMV-mCherry-Caprin1

LOCUS       pCDH-CMV-mCherry-Caprin1               9276 bp DNA     circular SYN 26-APR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 3, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1904..1953
                     /label=Primer 4
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2496..3203
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2909..2928
                     /label=mCherry-F
     misc_feature    3204..3232
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    3219..3257
                     /label=Primer F for CAPRIN1 in PCDH-mCherry
     CDS             3240..5366
                     /codon_start=1
                     
                     /translation="PSATSHSGSGSKSSGPPPPSGSSGSEAAAGAGAAAPASQHPATGT
                     GAVQTEAMKQILGVIDKKLRNLEKKKGKLDDYQERMNKGERLNQDQLDAVSKYQEVTNN
                     LEFAKELQRSFMALSQDIQKTIKKTARREQLMREEAEQKRLKTVLELQYVLDKLGDDEV
                     RTDLKQGLNGVPILSEEELSLLDEFYKLVDPERDMSLRLNEQYEHASIHLWDLLEGKEK
                     PVCGTTYKVLKEIVERVFQSNYFDSTHNHQNGLCEEEEAASAPAVEDQVPEAEPEPAEE
                     YTEQSEVESTEYVNRQFMAETQFTSGEKEQVDEWTVETVEVVNSLQQQPQAASPSVPEP
                     HSLTPVAQADPLVRRQRVQDLMAQMQGPYNFIQDSMLDFENQTLDPAIVSAQPMNPTQN
                     MDMPQLVCPPVHSESRLAQPNQVPVQPEATQVPLVSSTSEGYTASQPLYQPSHATEQRP
                     QKEPIDQIQATISLNTDQTTASSSLPAASQPQVFQAGTSKPLHSSGINVNAAPFQSMQT
                     VFNMNAPVPPVNEPETLKQQNQYQASYNQSFSSQPHQVEQTELQQEQLQTVVGTYHGSP
                     DQSHQVTGNHQQPPQQNTGFPRSNQPYYNSRGVSRGGSRGARGLMNGYRGPANGFRGGY
                     DGYRPSFSNTPNSGYTQSQFSAPRDYSGYQRDGYQQNFKRGSGQSGPRGAPRGRGGPPR
                     PNRGMPQMNTQQVN"
     misc_feature    complement(5349..5387)
                     /label=Primer R for CAPRIN1 in PCDH-mCherry
     misc_feature    5373..5960
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5844..5855)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6033..6266
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6338..6459
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6478..6613
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6651..6667)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6675..6691
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6699..6729)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6744..6765
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7053..7641)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7812..8672)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8673..8777)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9251..9267
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     2521 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2701 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2761 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2821 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2881 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2941 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3001 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3061 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3121 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3181 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcggaattcc
     3241 cctcggccac cagccacagc gggagcggca gcaagtcgtc cggaccgcca ccgccgtcgg
     3301 gttcctccgg gagtgaggcg gccgcgggag ccggggccgc cgcgccggct tctcagcacc
     3361 ccgcaaccgg caccggcgct gtccagaccg aggccatgaa gcagattctc ggggtgatcg
     3421 acaagaaact tcggaacctg gagaagaaaa agggtaagct tgatgattac caggaacgaa
     3481 tgaacaaagg ggaaaggctt aatcaagatc agctggatgc cgtttctaag taccaggaag
     3541 tcacaaataa tttggagttt gcaaaagaat tacagaggag tttcatggca ctaagtcaag
     3601 atattcagaa aacaataaag aagacagcac gtcgggagca gcttatgaga gaagaagctg
     3661 aacagaaacg tttaaaaact gtacttgagc tacagtatgt tttggacaaa ttgggagatg
     3721 atgaagtgcg gactgacctg aaacaaggtt tgaatggagt gccaatattg tccgaagagg
     3781 agttgtcatt gttggatgaa ttctataagc tagtagaccc tgaacgggac atgagcttga
     3841 ggttgaatga acagtatgaa catgcctcca ttcacctgtg ggacctgctg gaagggaagg
     3901 aaaaacctgt atgtggaacc acctataaag ttctaaagga aattgttgag cgtgtttttc
     3961 agtcaaacta ctttgacagc acccacaacc accagaatgg gctgtgtgag gaagaagagg
     4021 cagcctcagc acctgcagtt gaagaccagg tacctgaagc tgaacctgag ccagcagaag
     4081 agtacactga gcaaagtgaa gttgaatcaa cagagtatgt aaatagacag ttcatggcag
     4141 aaacacagtt caccagtggt gaaaaggagc aggtagatga gtggacagtt gaaacggttg
     4201 aggtggtaaa ttcactccag cagcaacctc aggctgcatc cccttcagta ccagagcccc
     4261 actctttgac tccagtggct caggcagatc cccttgtgag aagacagcga gtacaagacc
     4321 ttatggcaca aatgcagggt ccctataatt tcatacagga ttcaatgctg gattttgaaa
     4381 atcagacact tgatcctgcc attgtatctg cacagcctat gaatccaaca caaaacatgg
     4441 acatgcccca gctggtttgc cctccagttc attctgaatc tagacttgct cagcctaatc
     4501 aagttcctgt acaaccagaa gcgacacagg ttcctttggt atcatccaca agtgaggggt
     4561 acacagcatc tcaacccttg taccagcctt ctcatgctac agagcaacga ccacagaagg
     4621 aaccaattga tcagattcag gcaacaatct ctttaaatac agaccagact acagcatcat
     4681 catcccttcc tgctgcgtct cagcctcaag tatttcaggc tgggacaagc aaacctttac
     4741 atagcagtgg aatcaatgta aatgcagctc cattccaatc catgcaaacg gtgttcaata
     4801 tgaatgcccc agttcctcct gttaatgaac cagaaacttt aaaacagcaa aatcagtacc
     4861 aggccagtta taaccagagc ttttctagtc agcctcacca agtagaacaa acagagcttc
     4921 agcaagaaca gcttcaaaca gtggttggca cttaccatgg ttccccagac cagtcccatc
     4981 aagtgactgg taaccaccag cagcctcctc agcagaacac tggatttcca cgtagcaatc
     5041 agccctatta caatagtcgt ggtgtgtctc gtggaggctc ccgtggtgct agaggcttga
     5101 tgaatggata ccggggccct gccaatggat tcagaggagg atatgatggt taccgccctt
     5161 cattctctaa cactccaaac agtggttata cacagtctca gttcagtgct ccccgggatt
     5221 actctggcta tcaacgggat ggatatcagc agaatttcaa gcgaggctct gggcagagtg
     5281 gaccacgggg agccccacga ggtcgtggag ggcccccaag acccaacaga gggatgccgc
     5341 aaatgaacac tcagcaagtg aattaaggat ccaatcaacc tctggattac aaaatttgtg
     5401 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     5461 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     5521 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     5581 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     5641 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     5701 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     5761 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     5821 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     5881 tgctgccggc tctgcggcct cttccgcgtc tccgccttcg ccctcagacg agtcggatct
     5941 ccctttggcc gcctccccgc cggtaccttt aagaccaatg acttacaagg cagctgtaga
     6001 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa
     6061 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     6121 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6181 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6241 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     6301 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     6361 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6421 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     6481 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     6541 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     6601 ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg
     6661 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6721 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6781 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6841 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6901 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6961 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7021 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7081 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7141 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7201 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7261 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7321 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7381 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7441 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     7501 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7561 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7621 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7681 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7741 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7801 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7861 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7921 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     7981 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8041 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8101 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8161 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8221 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8281 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8341 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8401 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8461 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8521 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8581 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     8641 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     8701 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     8761 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     8821 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     8881 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     8941 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     9001 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     9061 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
     9121 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     9181 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     9241 tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//