| 质粒名称: | pAAVLINK-3'-CEP290-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001720 |
| 产品用途: | 3' AAVLINK plasmid for CEP290 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: |
CEP290
|
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-3'-CEP290-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-3'-CEP290-FLAG载体质粒基本信息
pAAVLINK-3'-CEP290-FLAG质粒图谱载体图谱和pAAVLINK-3'-CEP290-FLAG载体序列质粒序列多克隆位点信息
pAAVLINK-3'-CEP290-FLAG质粒载体简介
pAAVLINK-3'-CEP290-FLAG质粒序列载体序列
LOCUS pAAVLINK-3'-CEP290-FLAG 7227 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7227)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7227
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
protein_bind 145..178
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..3966
/codon_start=1
/label=CEP290
/translation="ALYYARLEGRNRAKHLRQTIQSLRRQFSGALPLAQQEKFSKTMIQ
LQNDKLKIMQEMKNSQQEHRNMENKTLEMELKLKGLEELISTLKDTKGAQKVINWHMKI
EELRLQELKLNRELVKDKEEIKYLNNIISEYERTISSLEEEIVQQNKFHEERQMAWDQR
EVDLERQLDIFDRQQNEILNAAQKFEEATGSIPDPSLPLPNQLEIALRKIKENIRIILE
TRATCKSLEEKLKEKESALRLAEQNILSRDKVINELRLRLPATAEREKLIAELGRKEME
PKSHHTLKIAHQTIANMQARLNQKEEVLKKYQRLLEKAREEQREIVKKHEEDLHILHHR
LELQADSSLNKFKQTAWDLMKQSPTPVPTNKHFIRLAEMEQTVAEQDDSLSSLLVKLKK
VSQDLERQREITELKVKEFENIKLQLQENHEDEVKKVKAEVEDLKYLLDQSQKESQCLK
SELQAQKEANSRAPTTTMRNLVERLKSQLALKEKQQKALSRALLELRAEMTAAAEERII
SATSQKEAHLNVQQIVDRHTRELKTQVEDLNENLLKLKEALKTSKNRENSLTDNLNDLN
NELQKKQKAYNKILREKEEIDQENDELKRQIKRLTSGLQGKPLTDNKQSLIEELQRKVK
KLENQLEGKVEEVDLKPMKEKNAKEELIRWEEGKKWQAKIEGIRNKLKEKEGEVFTLTK
QLNTLKDLFAKADKEKLTLQRKLKTTGMTVDQVLGIRALESEKELEELKKRNLDLENDI
LYMRAHQALPRDSVVEDLHLQNRYLQEKLHALEKQFSKDTYSKPSISGIESDDHCQREQ
ELQKENLKLSSENIELKFQLEQANKDLPRLKNQVRDLKEMCEFLKKEKAEVQRKLGHVR
GSGRSGKTIPELEKTIGLMKKVVEKVQRENEQLKKASGILTSEKMANIEQENEKLKAEL
EKLKAHLGHQLSMHYESKTKGTEKIIAENERLRKELKKETDAAEKLRIAKNNLEILNEK
MTVQLEETGKRLQFAESRGPQLEGADSKSWKSIVVTRMYETKLKELETDIAKKNQSITD
LKQLVKEATEREQKVNKYNEDLEQQIKILKHVPEGAETEQGLKRELQVLRLANHQLDKE
KAELIHQIEANKDQSGAESTIPDADQLKEKIKDLETQLKMSDLEKQHLKEEIKKLKKEL
ENFDPSFFEEIEDLKYNYKEEVKKNILLEEKVKKLSEQLGVELTSPVAASEEFEDEEES
PVNFPIY"
misc_feature 3737..3742
/label=SDS
CDS 3973..4038
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4042..4473
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4186..4191
/label=SDS
repeat_region 4490..4630
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4490..4619
/label=AAV2 ITR
rep_origin 4705..5160
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5442..5546
/gene="bla"
/label=AmpR promoter
CDS 5547..6407
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6578..7166
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag gctctctatt atgctcgttt ggagggaaga
301 aacagagcaa aacatctgcg ccaaacaatt cagtctctac gacgacagtt tagtggagct
361 ttacccttgg cacaacagga aaagttctcc aaaacaatga ttcaactaca aaatgacaaa
421 cttaagataa tgcaagaaat gaaaaattct caacaagaac atagaaatat ggagaacaaa
481 acattggaga tggaattaaa attaaagggc ctggaagagt taataagcac tttaaaggat
541 accaaaggag cccaaaaggt aatcaactgg catatgaaaa tagaagaact tcgtcttcaa
601 gaacttaaac taaatcggga attagtcaag gataaagaag aaataaaata tttgaataac
661 ataatttctg aatatgaacg tacaatcagc agtcttgaag aagaaattgt gcaacagaac
721 aagtttcatg aagaaagaca aatggcctgg gatcaaagag aagttgacct ggaacgccaa
781 ctagacattt ttgaccgtca gcaaaatgaa atactaaatg cggcacaaaa gtttgaagaa
841 gctacaggat caatccctga ccctagtttg ccccttccaa atcaacttga gatcgctcta
901 aggaaaatta aggagaacat tcgaataatt ctagaaacac gggcaacttg caaatcacta
961 gaagagaaac taaaagagaa agaatctgct ttaaggttag cagaacaaaa tatactgtca
1021 agagacaaag taatcaatga actgaggctt cgattgcctg ccactgcaga aagagaaaag
1081 ctcatagctg agctaggcag aaaagagatg gaaccaaaat ctcaccacac attgaaaatt
1141 gctcatcaaa ccattgcaaa catgcaagca aggttaaatc aaaaagaaga agtattaaag
1201 aagtatcaac gtcttctaga aaaagccaga gaggagcaaa gagaaattgt gaagaaacat
1261 gaggaagacc ttcatattct tcatcacaga ttagaactac aggctgatag ttcactaaat
1321 aaattcaaac aaacggcttg ggatttaatg aaacagtctc ccactccagt tcctaccaac
1381 aagcatttta ttcgtctggc tgagatggaa cagacagtag cagaacaaga tgactctctt
1441 tcctcactct tggtcaaact aaagaaagta tcacaagatt tggagagaca aagagaaatc
1501 actgaattaa aagtaaaaga atttgaaaat atcaaattac agcttcaaga aaaccatgaa
1561 gatgaagtga aaaaagtaaa agcggaagta gaggatttaa agtatcttct ggaccagtca
1621 caaaaggagt cacagtgttt aaaatctgaa cttcaggctc aaaaagaagc aaattcaaga
1681 gctccaacaa ctacaatgag aaatctagta gaacggctaa agagccaatt agccttgaag
1741 gagaaacaac agaaagcact tagtcgggca cttttagaac tccgggcaga aatgacagca
1801 gctgctgaag aacgtattat ttctgcaact tctcaaaaag aggcccatct caatgttcaa
1861 caaatcgttg atcgacatac tagagagcta aagacacaag ttgaagattt aaatgaaaat
1921 cttttaaaat tgaaagaagc acttaaaaca agtaaaaaca gagaaaactc actaactgat
1981 aatttgaatg acttaaataa tgaactgcaa aagaaacaaa aagcctataa taaaatactt
2041 agagagaaag aggaaattga tcaagagaat gatgaactga aaaggcaaat taaaagacta
2101 accagtggat tacagggcaa acccctgaca gataataaac aaagtctaat tgaagaactc
2161 caaaggaaag ttaaaaaact agagaaccaa ttagagggaa aggtggagga agtagaccta
2221 aaacctatga aagaaaagaa tgctaaagaa gaattaatta ggtgggaaga aggtaaaaag
2281 tggcaagcca aaatagaagg aattcgaaac aagttaaaag agaaagaggg ggaagtcttt
2341 actttaacaa agcagttgaa tactttgaag gatctttttg ccaaagccga taaagagaaa
2401 cttactttgc agaggaaact aaaaacaact ggcatgactg ttgatcaggt tttgggaata
2461 cgagctttgg agtcagaaaa agaattggaa gaattaaaaa agagaaatct tgacttagaa
2521 aatgatatat tgtatatgag ggcccaccaa gctcttcctc gagattctgt tgtagaagat
2581 ttacatttac aaaatagata cctccaagaa aaacttcatg ctttagaaaa acagttttca
2641 aaggatacat attctaagcc ttcaatttca ggaatagagt cagatgatca ttgtcagaga
2701 gaacaggagc ttcagaagga aaacttgaag ttgtcatctg aaaatattga actgaaattt
2761 cagcttgaac aagcaaataa agatttgcca agattaaaga atcaagtcag agatttgaag
2821 gaaatgtgtg aatttcttaa gaaagaaaaa gcagaagttc agcggaaact tggccatgtt
2881 agagggtctg gtagaagtgg aaagacaatc ccagaactgg aaaaaaccat tggtttaatg
2941 aaaaaagtag ttgaaaaagt ccagagagaa aatgaacagt tgaaaaaagc atcaggaata
3001 ttgactagtg aaaaaatggc taatattgag caggaaaatg aaaaattgaa ggctgaatta
3061 gaaaaactta aagctcatct tgggcatcag ttgagcatgc actatgaatc caagaccaaa
3121 ggcacagaaa aaattattgc tgaaaatgaa aggcttcgta aagaacttaa aaaagaaact
3181 gatgctgcag agaaattacg gatagcaaag aataatttag agatattaaa tgagaagatg
3241 acagttcaac tagaagagac tggtaagaga ttgcagtttg cagaaagcag aggtccacag
3301 cttgaaggtg ctgacagtaa gagctggaaa tccattgtgg ttacaagaat gtatgaaacc
3361 aagttaaaag aattggaaac tgatattgcc aaaaaaaatc aaagcattac tgaccttaaa
3421 cagcttgtaa aagaagcaac agagagagaa caaaaagtta acaaatacaa tgaagacctt
3481 gaacaacaga ttaagattct taaacatgtt cctgaaggtg ctgagacaga gcaaggcctt
3541 aaacgggagc ttcaagttct tagattagct aatcatcagc tggataaaga gaaagcagaa
3601 ttaatccatc agatagaagc taacaaggac caaagtggag ctgaaagcac catacctgat
3661 gctgatcaac taaaggaaaa aataaaagat ctagagacac agctcaaaat gtcagatcta
3721 gaaaagcagc atttgaagga ggaaataaag aagctgaaaa aagaactgga aaattttgat
3781 ccttcatttt ttgaagaaat tgaagatctt aagtataatt acaaggaaga agtgaagaag
3841 aatattctct tagaagagaa ggtaaaaaaa ctttcagaac aattgggagt tgaattaact
3901 agccctgttg ctgcttctga agagtttgaa gatgaagaag aaagtcctgt taatttcccc
3961 atttactgta cagactacaa agaccatgac ggtgattata aagatcatga catcgactac
4021 aaggatgacg atgacaagta actcgagata atcaacctct ggattacaaa atttgtgaaa
4081 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
4141 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
4201 cctggttagt tcttgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
4261 gggctcggct gttgggcact gacaattccg tggtgtttat ttgtgaaatt tgtgatgcta
4321 ttgctttatt tgtaaccatc tagctttatt tgtgaaattt gtgatgctat tgctttattt
4381 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
4441 caggttcagg gggagatgtg ggaggttttt taacggaccg agcggccgca ggaaccccta
4501 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
4561 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
4621 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
4681 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
4741 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg
4801 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
4861 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
4921 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
4981 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta
5041 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa
5101 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
5161 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
5221 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
5281 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
5341 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
5401 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
5461 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
5521 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
5581 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
5641 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
5701 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
5761 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
5821 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
5881 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
5941 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
6001 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
6061 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
6121 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
6181 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
6241 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
6301 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
6361 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
6421 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
6481 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
6541 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
6601 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
6661 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
6721 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
6781 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
6841 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
6901 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
6961 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
7021 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
7081 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
7141 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
7201 ccttttgctg gccttttgct cacatgt
//
