pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SBF1-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SBF1-FLAG                7141 bp DNA     circular SYN 25-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3854
                     /codon_start=2
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /product="myotubularin-related protein 5 isoform 1"
                     /label=SBF1
                     /note="isoform 1 is encoded by transcript variant 1; 
                     myotubularin-related protein 5; DENN/MADD domain containing
                     7A; inactive phosphatidylinositol 3-phosphatase 5"
                     /db_xref="GI:239735519"
                     /db_xref="CCDS:CCDS14091.2"
                     /db_xref="GeneID:6305"
                     /db_xref="HGNC:HGNC:10542"
                     /db_xref="MIM:603560"
                     /protein_id="NP_002963.2"
                     /translation="ACCRDYQRLGLGTLSSSLSRAKSEPFRISPVNRMYAICRSYPGLL
                     IVPQSVQDNALQRVSRCYRQNRFPVVCWRSGRSKAVLLRSGGLHGKGVVGLFKAQNAPS
                     PGQSQADSSSLEQEKYLQAVVSSMPRYADASGRNTLSGFSSAHMGSHVPSPRARVTTLS
                     NPMAASASRRTAPRGKWGSVRTSGRSSGLGTDVGSRLAGRDALAPPQANGGPPDPGFLR
                     PQRAALYILGDKAQLKGVRSDPLQQWELVPIEVFEARQVKASFKKLLKACVPGCPAAEP
                     SPASFLRSLEDSEWLIQIHKLLQVSVLVVELLDSGSSVLVGLEDGWDITTQVVSLVQLL
                     SDPFYRTLEGFRLLVEKEWLSFGHRFSHRGAHTLAGQSSGFTPVFLQFLDCVHQVHLQF
                     PMEFEFSQFYLKFLGYHHVSRRFRTFLLDSDYERIELGLLYEEKGERRGQVPCRSVWEY
                     VDRLSKRTPVFHNYMYAPEDAEVLRPYSNVSNLKVWDFYTEETLAEGPPYDWELAQGPP
                     EPPEEERSDGGAPQSRRRVVWPCYDSCPRAQPDAISRLLEELQRLETELGQPAERWKDT
                     WDRVKAAQRLEGRPDGRGTPSSLLVSTAPHHRRSLGVYLQEGPVGSTLSLSLDSDQSSG
                     STTSGSRQAARRSTSTLYSQFQTAESENRSYEGTLYKKGAFMKPWKARWFVLDKTKHQL
                     RYYDHRVDTECKGVIDLAEVEAVAPGTPTMGAPKTVDEKAFFDVKTTRRVYNFCAQDVP
                     SAQQWVDRIQSCLSDA"
     regulatory      2041..2050
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2398..2403
                     /label=SDS
     misc_feature    3341..3346
                     /label=SDS
     misc_feature    3394..3399
                     /label=SDS
     CDS             3861..3926
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3930..4361
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4074..4079
                     /label=SDS
     repeat_region   4404..4544
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4404..4533
                     /label=AAV2 ITR
     repeat_region   4405..4545
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4619..5074
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5356..5460
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5461..6321
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6492..7080
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggct tgctgtcgcg
     1561 actaccagcg cctcggtctg ggcaccctga gcagcagcct gagccgggcc aagtctgagc
     1621 ccttccgcat ttctccggtc aaccgcatgt atgccatctg ccgcagctac ccagggctgc
     1681 tgatcgtgcc ccagagtgtc caggacaacg ccctgcagcg cgtgtcccgc tgctaccgcc
     1741 agaaccgctt ccccgtggtc tgctggcgca gcgggcggtc caaggcggtg ctgctgcgct
     1801 ctggaggcct gcatggcaaa ggtgtcgtcg gcctcttcaa ggcccagaac gcaccttctc
     1861 caggccagtc ccaggcggac tcgagtagcc tggagcagga gaagtacctg caggctgtgg
     1921 tcagctccat gccccgctac gccgacgcgt cgggacgcaa cacgcttagc ggcttctcct
     1981 cagcccacat gggcagtcac gttcccagcc ccagagccag ggtcaccacg ctgtccaacc
     2041 ccatggcggc ctcggcctcc agacggaccg caccccgagg taagtggggc agtgtccgga
     2101 ccagtggacg cagcagtggc cttggcaccg atgtgggctc ccggctagct ggcagagacg
     2161 cgctggcccc accccaggcc aacgggggcc ctcccgaccc gggcttcctg cgtccgcagc
     2221 gagcagccct ctatatcctt ggggacaaag cccagctcaa gggtgtgcgg tcagaccccc
     2281 tgcagcagtg ggagctggtg cccattgagg tattcgaggc acggcaggtg aaggctagct
     2341 tcaagaagct gctgaaagca tgtgtcccag gctgccccgc tgctgagccc agcccagcct
     2401 ccttcctgcg ctcactggag gactcagagt ggctgatcca gatccacaag ctgctgcagg
     2461 tgtctgtgct ggtggtggag ctcctggatt caggctcctc cgtgctggtg ggcctggagg
     2521 atggctggga catcaccacc caggtggtat ccttggtgca gctgctctca gaccccttct
     2581 accgcacgct ggagggcttt cgcctgctgg tggagaagga gtggctgtcc ttcggccatc
     2641 gcttcagcca ccgtggagct cacaccctgg ccgggcagag cagcggcttc acacccgtct
     2701 tcctgcagtt cctggactgc gtacaccagg tccacctgca gttccccatg gagtttgagt
     2761 tcagccagtt ctacctcaag ttcctcggct accaccatgt gtcccgccgt ttccggacct
     2821 tcctgctcga ctctgactat gagcgcattg agctggggct gctgtatgag gagaaggggg
     2881 aacgcagggg ccaggtgccg tgcaggtctg tgtgggagta tgtggaccgg ctgagcaaga
     2941 ggacgcctgt gttccacaat tacatgtatg cgcccgagga cgcagaggtc ctgcggccct
     3001 acagcaacgt gtccaacctg aaggtgtggg acttctacac tgaggagacg ctggccgagg
     3061 gccctcccta tgactgggaa ctggcccagg ggccccctga acccccagag gaagaacggt
     3121 ctgatggagg cgctccccag agcaggcgcc gcgtggtgtg gccctgttac gacagctgcc
     3181 cgcgggccca gcctgacgcc atctcacgcc tgctggagga gctgcagagg ctggagacag
     3241 agttgggcca acccgctgag cgctggaagg acacctggga ccgggtgaag gctgcacagc
     3301 gcctcgaggg ccggccagac ggccgtggca cccctagctc cctccttgtg tccaccgcac
     3361 cccaccaccg tcgctcgctg ggtgtgtacc tgcaggaggg gcccgtgggc tccaccctga
     3421 gcctcagcct ggacagcgac cagagtagtg gctcaaccac atccggctcc cgtcaggctg
     3481 cccgccgcag caccagcacc ctgtacagcc agttccagac agcagagagt gagaacaggt
     3541 cctacgaggg cactctgtac aagaaggggg ccttcatgaa gccttggaag gcccgctggt
     3601 tcgtgctgga caagaccaag caccagctgc gctactacga ccaccgtgtg gacacagagt
     3661 gcaagggtgt catcgacttg gcggaggtgg aggctgtggc acctggcacg cccactatgg
     3721 gtgcccctaa gaccgtggac gagaaggcct tctttgacgt gaagacaacg cgtcgcgttt
     3781 acaacttctg tgcccaggac gtgccctcgg cccagcagtg ggtggaccgg atccagagct
     3841 gcctgtcgga cgcctgtaca gactacaaag accatgacgg tgattataaa gatcatgaca
     3901 tcgactacaa ggatgacgat gacaagtaac tcgagataat caacctctgg attacaaaat
     3961 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     4021 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4081 gtataaatcc tggttagttc ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4141 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgtttattt gtgaaatttg
     4201 tgatgctatt gctttatttg taaccatcta gctttatttg tgaaatttgt gatgctattg
     4261 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     4321 ttatgtttca ggttcagggg gagatgtggg aggtttttta aagatctgga gaccggtgtg
     4381 gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     4441 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     4501 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     4561 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     4621 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4681 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4741 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4801 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     4861 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4921 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
     4981 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5041 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     5101 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     5161 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     5221 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     5281 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     5341 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5401 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5461 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5521 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5581 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5641 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5701 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5761 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5821 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5881 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5941 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     6001 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     6061 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     6121 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6181 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6241 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6301 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6361 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6421 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6481 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6541 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6601 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6661 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6721 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6781 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6841 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6901 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6961 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7021 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7081 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7141 t
//