| 质粒名称: | pAAV-hSyn-GRAB_HA1m |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000150 |
| 产品用途: | Express the medium-affinity histamine sensor GRAB_HA1m in neurons |
| 存储实验室: | Yulong Li Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | hSyn |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | GRAB_HA1m |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Genetically encoded sensors for measuring histamine release both in vitro and in vivo
Dong H., Li M., Yan Y., Qian T., Lin Y., Ma X., Vischer H.F., Liu C., Li G., Wang H., Leurs R., Li Y.
Neuron 111, 1564-1576.e6 (2023)
|
买家导航
pAAV-hSyn-GRAB_HA1m载体质粒基本信息
pAAV-hSyn-GRAB_HA1m质粒图谱载体图谱和pAAV-hSyn-GRAB_HA1m载体序列质粒序列多克隆位点信息
pAAV-hSyn-GRAB_HA1m质粒载体简介
pAAV-hSyn-GRAB_HA1m质粒序列载体序列
LOCUS pAAV-hSyn-GRAB_HA1m 6539 bp DNA circular SYN 21-APR-2023
DEFINITION Express the medium-affinity histamine sensor GRAB_HA1m in neurons.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6539)
AUTHORS Dong H, Li M, Yan Y, Qian T, Lin Y, Ma X, Vischer HF, Liu C, Li G,
Wang H, Leurs R, Li Y
TITLE Genetically encoded sensors for measuring histamine release both in
vitro and in vivo
JOURNAL bioRxiv 2022.08.19.504485
REFERENCE 2 (bases 1 to 6539)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv
2022.08.19.504485"
FEATURES Location/Qualifiers
source 1..6539
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
promoter 131..574
/label=hSyn
regulatory 581..590
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
sig_peptide 587..649
/codon_start=1
/product="leader sequence from mouse immunoglobulin kappa
light chain"
/label=Ig-kappa leader
/translation="METDTLLLWVLLLWVPGSTGD"
protein_bind 650..674
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 674..2641
/codon_start=1
/label=GRAB_HA1m
/translation="TTGMEPAGAGGAAILLDDDDDYYLTTPGYPGSGSNASLSPPADLF
PAASNHSTAVVVSGDLDLYVIIPLGIFLAVLSLVTFAGNVMVLHALRTEKKLQTVSNIF
IMSLAIADCSVGLFAMPLSAVYALLGYWPFGLVVCNMWLSMDYVASTASIFNLFALSLD
RFWSIISPLRYLKKRTRKRAIIMCSSVWLASLLWIIPVVGWHQMFNDGRRDVPVEECDT
EFADEQVFKVLTAIFNFYVPMAAMIALYGRIFHEIKLRAASQPAPAGPRDTDALDLEEG
GNVYIKADKQKNGIKANFVIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSK
LSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVRKGEELFTGVVPILVELDG
DVNGHKFSVSGEGEGDATEGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMK
QHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILG
HKLEYNTGAAARWRAKKAAFQLGVILGAFCLCFMPYFISFIITAFCKDCVSPAVHSIFT
WLGYVNSTTNPFLYTWTNANFRSAFRKILRMKETPKKPVYMQMPHKTPRPKKQPLEARV
SGTASGGTTAPARKSDDTRL"
primer_bind 1280..1301
/label=WKG150-F
primer_bind 1726..1747
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(1853..1874)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2114..2133)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
misc_feature 2666..3254
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2719..2739)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3137..3148)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3286..3762
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(3485..3504)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
repeat_region 3802..3942
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 3802..3931
/label=AAV2 ITR
rep_origin 4017..4472
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4104..4123)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4314..4335
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4489..4508)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4608..4630
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4668..4686)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4754..4858
/gene="bla"
/label=AmpR promoter
CDS 4859..5719
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5077..5096)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5890..6478
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6379..6398
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtatgagg cggggtgggg gtgcctacct gacgaccgac
181 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag
241 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg
301 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg
361 ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc
421 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc
481 gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga
541 ggagtcgtgt cgtgcctgag agcgcagtcg agaaaccggt gccaccatgg agacagacac
601 actcctgcta tgggtactgc tgctctgggt tccaggttcc actggtgaca caagtttgta
661 caaaaaagtt ggcaccaccg gtatggagcc cgccggcgcc ggcggcgccg ccattctgct
721 ggacgacgac gacgactact acctcacaac ccccggctac cccggctccg gctctaacgc
781 ctccctgtct cctccagccg acctgttccc cgccgcctct aaccactcga ccgctgtcgt
841 ggtgtctggc gacctggacc tgtacgtgat cattccactg ggcatcttcc tcgccgtgct
901 gtctctcgtg acattcgccg gcaacgtgat ggtgctgcac gccctcagaa cagagaagaa
961 gctccaaaca gtgtctaaca ttttcatcat gtctctcgcc attgccgact gctctgtggg
1021 cctgttcgcc atgccactga gcgccgtgta cgccctgctg ggctactggc ctttcggcct
1081 cgtggtgtgc aacatgtggt tgagtatgga ctacgtggcc agcacagcct ctattttcaa
1141 cctgttcgcc ctgtccctcg accggttctg gtctattatt tctccactca gatacctcaa
1201 gaagcggaca cgcaagcggg ccattattat gtgctctagc gtgtggctcg cctctctcct
1261 gtggattatc ccggttgtag gatggcacca gatgttcaac gacggcaggc gggacgtgcc
1321 agtggaggag tgcgacaccg agttcgccga cgagcaggtg ttcaaggtgc tgacagccat
1381 cttcaacttc tacgtgccta tggccgccat gattgccctg tacggcagga ttttccacga
1441 gattaagctc agagccgcgt ctcagccagc accggccggg ccgcgcgaca ccgacgcgct
1501 ggacctggag gagggaggaa acgtctatat caaggccgac aagcagaaga acggcatcaa
1561 ggcgaacttc gtgatccgcc acaacatcga ggacggcggc gtgcagctcg cctaccacta
1621 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
1681 cgtgcagtcc aaactttcga aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
1741 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtaccgg
1801 agggagcatg gtgagaaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
1861 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggtg agggcgatgc
1921 caccgagggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
1981 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
2041 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacatcc aggagcgcac
2101 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
2161 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
2221 ggggcacaag ctggagtaca acaccggagc agcagcacgc tggcgcgcga agaaggccgc
2281 cttccagctg ggcgtgatcc tgggcgcctt ctgcctgtgc ttcatgcctt acttcattag
2341 cttcatcatt accgccttct gcaaggactg cgtgtctcct gccgtgcact ctattttcac
2401 atggctgggc tacgtgaact ctacaacaaa ccctttcctg tacacatgga caaacgccaa
2461 cttccggtcc gccttccgga agatcctccg gatgaaggag acaccaaaga agccagtgta
2521 catgcagatg cctcacaaga cccctagacc aaagaagcag ccattagaag ctagggtgag
2581 cggcacagcc tctggcggca ccacagcccc agcccgcaag tctgacgaca ccagactcta
2641 agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
2701 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
2761 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
2821 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
2881 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
2941 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
3001 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
3061 atcatcgtcc tttccttggc tgctcgccta tgttgccacc tggattctgc gcgggacgtc
3121 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
3181 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
3241 ggccgcctcc ccgcatcgat accgagcgct gctcgagaga tctacgggtg gcatccctgt
3301 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
3361 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
3421 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
3481 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
3541 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
3601 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
3661 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
3721 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt gtaggtaacc
3781 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
3841 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
3901 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
3961 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
4021 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
4081 acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
4141 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
4201 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
4261 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
4321 cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg
4381 gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc
4441 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
4501 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
4561 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
4621 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
4681 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
4741 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
4801 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
4861 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
4921 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
4981 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
5041 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
5101 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
5161 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
5221 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
5281 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
5341 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
5401 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
5461 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
5521 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
5581 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
5641 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
5701 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
5761 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
5821 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
5881 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
5941 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
6001 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
6061 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
6121 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
6181 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
6241 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
6301 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
6361 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
6421 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
6481 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
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