pSL1014

LOCUS       pSL1014            11497 bp DNA     circular SYN 11-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11497)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Aug 17, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    232..819
                     /label=CMV
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     863..882
                     /label=T7
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    895..900
                     /label=NheI
     misc_feature    911..916
                     /label=New Feature
     misc_feature    911..916
                     /label=New Feature
     primer_bind     938..984
                     /label=oSCL1926
     misc_feature    952..956
                     /label=EcoRI
     regulatory      958..967
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             964..3744
                     /codon_start=1
                     /label=BCR
                     /translation="MVDPVGFAEAWKAQFPDSEPPRMELRSVGDIEQELERCKASIRRL
                     EQEVNQERFRMIYLQTLLAKEKKSYDRQRWGFRRAAQAPDGASEPRASASRPQPAPADG
                     ADPPPAEEPEARPDGEGSPGKARPGTARRPGAAASGERDDRGPPASVAALRSNFERIRK
                     GHGQPGADAEKPFYVNVEFHHERGLVKVNDKEVSDRISSLGSQAMQMERKKSQHGAGSS
                     VGDASRPPYRGRSSESSCGVDGDYEDAELNPRFLKDNLIDANGGSRPPWPPLEYQPYQS
                     IYVGGMMEGEGKGPLLRSQSTSEQEKRLTWPRRSYSPRSFEDCGGGYTPDCSSNENLTS
                     SEEDFSSGQSSRVSPSPTTYRMFRDKSRSPSQNSQQSFDSSSPPTPQCHKRHRHCPVVV
                     SEATIVGVRKTGQIWPNDGEGAFHGDADGSFGTPPGYGCAADRAEEQRRHQDGLPYIDD
                     SPSSSPHLSSKGRGSRDALVSGALESTKASELDLEKGLEMRKWVLSGILASEETYLSHL
                     EALLLPMKPLKAAATTSQPVLTSQQIETIFFKVPELYEIHKEFYDGLFPRVQQWSHQQR
                     VGDLFQKLASQLGVYRAFVDNYGVAMEMAEKCCQANAQFAEISENLRARSNKDAKDPTT
                     KNSLETLLYKPVDRVTRSTLVLHDLLKHTPASHPDHPLLQDALRISQNFLSSINEEITP
                     RRQSMTVKKGEHRQLLKDSFMVELVEGARKLRHVFLFTDLLLCTKLKKQSGGKTQQYDC
                     KWYIPLTDLSFQMVDELEAVPNIPLVPDEELDALKIKISQIKSDIQREKRANKGSKATE
                     RLKKKLSEQESLLLLMSPSMAFRVHSRNGKSYTFLISSDYERAEWRENIREQQKKCFRS
                     FSLTSVELQMLTNSCVKLQTVHSIPLTINKEDDESPGLYGFLNVIVHSATGFKQSS"
     primer_bind     964..984
                     /label=BCR-1
     primer_bind     1793..1812
                     /label=BCR-2
     primer_bind     2665..2682
                     /label=BCR-3
     primer_bind     3490..3507
                     /label=BCR-4
     CDS             3748..7056
                     /codon_start=1
                     /product="NP_005148.2 tyrosine-protein kinase ABL1 isoform 
                     a [Homo sapiens]"
                     /label=ABL
                     /translation="ALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDF
                     VASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGP
                     VSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSES
                     RFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTVYGVSPNYDKWEMERTDITMKHKLG
                     GGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTRE
                     PPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAAR
                     NCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFG
                     VLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPS
                     FAEIHQAFETMFQESSISDEVEKELGKQGVRGAVSTLLQAPELPTKTRTSRRAAEHRDT
                     TDVPEMPHSKGQGESDPLDHEPAVSPLLPRKERGPPEGGLNEDERLLPKDKKTNLFSAL
                     IKKKKKTAPTPPKRSSSFREMDGQPERRGAGEEEGRDISNGALAFTPLDTADPAKSPKP
                     SNGAGVPNGALRESGGSGFRSPHLWKKSSTLTSSRLATGEEEGGGSSSKRFLRSCSASC
                     VPHGAKDTEWRSVTLPRDLQSTGRQFDSSTFGGHKSEKPALPRKRAGENRSDQVTRGTV
                     TPPPRLVKKNEEAADEVFKDIMESSPGSSPPNLTPKPLRRQVTVAPASGLPHKEEAGKG
                     SALGTPAAAEPVTPTSKAGSGAPGGTSKGPAEESRVRRHKHSSESPGRDKGKLSRLKPA
                     PPPPPAASAGKAGGKPSQSPSQEAAGEAVLGAKTKATSLVDAVNSDAAKPSQPGEGLKK
                     PVLPATPKPQSAKPSGTPISPAPVPSTLPSASSALAGDQPSSTAFIPLISTRVSLRKTR
                     QPPERIASGAITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQ
                     QMRNKFAFREAINKLENNLRELQICPATAGSGPAATQDFSKLLSSVKEISDIVQR"
     primer_bind     4303..4323
                     /label=ABL-1
     primer_bind     5125..5142
                     /label=ABL-2
     primer_bind     6030..6049
                     /label=ABL-3
     primer_bind     6811..6827
                     /label=ABL-4
     primer_bind     complement(7038..7079)
                     /label=oSCL1927
     misc_feature    7061..7065
                     /label=New Feature
     misc_feature    7061..7065
                     /label=New Feature
     terminator      7094..7321
                     /label=bGH PA
     polyA_signal    7097..7321
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7367..7795
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7809..8138
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7989..8124
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    7989..8066
                     /label=SV40 ORI
     CDS             8205..8999
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8208..8999
                     /label=Kan/neoR
     polyA_signal    9173..9294
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9339..9359)
                     /label=M13-rev
     primer_bind     complement(9343..9359)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(9365..9387)
                     /label=LacO
     protein_bind    9367..9383
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9391..9421)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        complement(9392..9421)
                     /label=lac
     protein_bind    9436..9457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9745..10330)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10501..11361)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11362..11466)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(11403..11431)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc
      961 accatggtgg acccggtggg cttcgcggag gcgtggaagg cgcagttccc ggactcagag
     1021 cccccgcgca tggagctgcg ctcagtgggc gacatcgagc aggagctgga gcgctgcaag
     1081 gcctccattc ggcgcctgga gcaggaggtg aaccaggagc gcttccgcat gatctacctg
     1141 cagacgttgc tggccaagga aaagaagagc tatgaccggc agcgatgggg cttccggcgc
     1201 gcggcgcagg cccccgacgg cgcctccgag ccccgagcgt ccgcgtcgcg cccgcagcca
     1261 gcgcccgccg acggagccga cccgccgccc gccgaggagc ccgaggcccg gcccgacggc
     1321 gagggttctc cgggtaaggc caggcccggg accgcccgca ggcccggggc agccgcgtcg
     1381 ggggaacggg acgaccgggg accccccgcc agcgtggcgg cgctcaggtc caacttcgag
     1441 cggatacgca agggccatgg ccagcccggg gcggacgccg agaagccctt ctacgtgaac
     1501 gtcgagtttc accacgagcg cggcctggtg aaggtcaacg acaaagaggt gtcggaccgc
     1561 atcagctccc tgggcagcca ggccatgcag atggagcgca aaaagtccca gcacggcgcg
     1621 ggctcgagcg tgggggatgc atccaggccc ccttaccggg gacgctcctc ggagagcagc
     1681 tgcggcgtcg acggcgacta cgaggacgcc gagttgaacc cccgcttcct gaaggacaac
     1741 ctgatcgacg ccaatggcgg tagcaggccc ccttggccgc ccctggagta ccagccctac
     1801 cagagcatct acgtcggggg catgatggaa ggggagggca agggcccgct cctgcgcagc
     1861 cagagcacct ctgagcagga gaagcgcctt acctggcccc gcaggtccta ctccccccgg
     1921 agttttgagg attgcggagg cggctatacc ccggactgca gctccaatga gaacctcacc
     1981 tccagcgagg aggacttctc ctctggccag tccagccgcg tgtccccaag ccccaccacc
     2041 taccgcatgt tccgggacaa aagccgctct ccctcgcaga actcgcaaca gtccttcgac
     2101 agcagcagtc cccccacgcc gcagtgccat aagcggcacc ggcactgccc ggttgtcgtg
     2161 tccgaggcca ccatcgtggg cgtccgcaag accgggcaga tctggcccaa cgatggcgag
     2221 ggcgccttcc atggagacgc agatggctcg ttcggaacac cacctggata cggctgcgct
     2281 gcagaccggg cagaggagca gcgccggcac caagatgggc tgccctacat tgatgactcg
     2341 ccctcctcat cgccccacct cagcagcaag ggcaggggca gccgggatgc gctggtctcg
     2401 ggagccctgg agtccactaa agcgagtgag ctggacttgg aaaagggctt ggagatgaga
     2461 aaatgggtcc tgtcgggaat cctggctagc gaggagactt acctgagcca cctggaggca
     2521 ctgctgctgc ccatgaagcc tttgaaagcc gctgccacca cctctcagcc ggtgctgacg
     2581 agtcagcaga tcgagaccat cttcttcaaa gtgcctgagc tctacgagat ccacaaggag
     2641 ttctatgatg ggctcttccc ccgcgtgcag cagtggagcc accagcagcg ggtgggcgac
     2701 ctcttccaga agctggccag ccagctgggt gtgtaccggg ccttcgtgga caactacgga
     2761 gttgccatgg aaatggctga gaagtgctgt caggccaatg ctcagtttgc agaaatctcc
     2821 gagaacctga gagccagaag caacaaagat gccaaggatc caacgaccaa gaactctctg
     2881 gaaactctgc tctacaagcc tgtggaccgt gtgacgagga gcacgctggt cctccatgac
     2941 ttgctgaagc acactcctgc cagccaccct gaccacccct tgctgcagga cgccctccgc
     3001 atctcacaga acttcctgtc cagcatcaat gaggagatca caccccgacg gcagtccatg
     3061 acggtgaaga agggagagca ccggcagctg ctgaaggaca gcttcatggt ggagctggtg
     3121 gagggggccc gcaagctgcg ccacgtcttc ctgttcaccg acctgcttct ctgcaccaag
     3181 ctcaagaagc agagcggagg caaaacgcag cagtatgact gcaaatggta cattccgctc
     3241 acggatctca gcttccagat ggtggatgaa ctggaggcag tgcccaacat ccccctggtg
     3301 cccgatgagg agctggacgc tttgaagatc aagatctccc agatcaagag tgacatccag
     3361 agagagaaga gggcgaacaa gggcagcaag gctacggaga ggctgaagaa gaagctgtcg
     3421 gagcaggagt cactgctgct gcttatgtct cccagcatgg ccttcagggt gcacagccgc
     3481 aacggcaaga gttacacgtt cctgatctcc tctgactatg agcgtgcaga gtggagggag
     3541 aacatccggg agcagcagaa gaagtgtttc agaagcttct ccctgacatc cgtggagctg
     3601 cagatgctga ccaactcgtg tgtgaaactc cagactgtcc acagcattcc gctgaccatc
     3661 aataaggaag atgatgagtc tccggggctc tatgggtttc tgaatgtcat cgtccactca
     3721 gccactggat ttaagcagag ttcaaaagcc cttcagcggc cagtagcatc tgactttgag
     3781 cctcagggtc tgagtgaagc cgctcgttgg aactccaagg aaaaccttct cgctggaccc
     3841 agtgaaaatg accccaacct tttcgttgca ctgtatgatt ttgtggccag tggagataac
     3901 actctaagca taactaaagg tgaaaagctc cgggtcttag gctataatca caatggggaa
     3961 tggtgtgaag cccaaaccaa aaatggccaa ggctgggtcc caagcaacta catcacgcca
     4021 gtcaacagtc tggagaaaca ctcctggtac catgggcctg tgtcccgcaa tgccgctgag
     4081 tatctgctga gcagcgggat caatggcagc ttcttggtgc gtgagagtga gagcagtcct
     4141 ggccagaggt ccatctcgct gagatacgaa gggagggtgt accattacag gatcaacact
     4201 gcttctgatg gcaagctcta cgtctcctcc gagagccgct tcaacaccct ggccgagttg
     4261 gttcatcatc attcaacggt ggccgacggg ctcatcacca cgctccatta tccagcccca
     4321 aagcgcaaca agcccactgt ctatggtgtg tcccccaact acgacaagtg ggagatggaa
     4381 cgcacggaca tcaccatgaa gcacaagctg ggcgggggcc agtacgggga ggtgtacgag
     4441 ggcgtgtgga agaaatacag cctgacggtg gccgtgaaga ccttgaagga ggacaccatg
     4501 gaggtggaag agttcttgaa agaagctgca gtcatgaaag agatcaaaca ccctaacctg
     4561 gtgcagctcc ttggggtctg cacccgggag cccccgttct atatcatcac tgagttcatg
     4621 acctacggga acctcctgga ctacctgagg gagtgcaacc ggcaggaggt gaacgccgtg
     4681 gtgctgctgt acatggccac tcagatctcg tcagccatgg agtacctgga gaagaaaaac
     4741 ttcatccaca gagatcttgc tgcccgaaac tgcctggtag gggagaacca cttggtgaag
     4801 gtagctgatt ttggcctgag caggttgatg acaggggaca cctacacagc ccatgctgga
     4861 gccaagttcc ccatcaaatg gactgcaccc gagagcctgg cctacaacaa gttctccatc
     4921 aagtccgacg tctgggcatt tggagtattg ctttgggaaa ttgctaccta tggcatgtcc
     4981 ccttacccgg gaattgacct gtcccaggtg tatgagctgc tagagaagga ctaccgcatg
     5041 gagcgcccag aaggctgccc agagaaggtc tatgaactca tgcgagcatg ttggcagtgg
     5101 aatccctctg accggccctc ctttgctgaa atccaccaag cctttgaaac aatgttccag
     5161 gaatccagta tctcagacga agtggaaaag gagctgggga aacaaggcgt ccgtggggct
     5221 gtgagtacct tgctgcaggc cccagagctg cccaccaaga cgaggacctc caggagagct
     5281 gcagagcaca gagacaccac tgacgtgcct gagatgcctc actccaaggg ccagggagag
     5341 agcgatcctc tggaccatga gcctgccgtg tctccattgc tccctcgaaa agagcgaggt
     5401 cccccggagg gcggcctgaa tgaagatgag cgccttctcc ccaaagacaa aaagaccaac
     5461 ttgttcagcg ccttgatcaa gaagaagaag aagacagccc caacccctcc caaacgcagc
     5521 agctccttcc gggagatgga cggccagccg gagcgcagag gggccggcga ggaagagggc
     5581 cgagacatca gcaacggggc actggctttc acccccttgg acacagctga cccagccaag
     5641 tccccaaagc ccagcaatgg ggctggggtc cccaatggag ccctccggga gtctgggggc
     5701 tcaggcttcc ggtctcccca cctgtggaag aagtccagca cgctgaccag cagccgccta
     5761 gccaccggcg aggaggaggg cggtggcagc tccagcaagc gcttcctgcg ctcttgctcc
     5821 gcctcctgcg ttccccatgg ggccaaggac acggagtgga ggtcagtcac gctgcctcgg
     5881 gacttgcagt ccacgggaag acagtttgac tcgtccacat ttggagggca caaaagtgag
     5941 aagccggctc tgcctcggaa gagggcaggg gagaacaggt ctgaccaggt gacccgaggc
     6001 acagtaacgc ctccccccag gctggtgaaa aagaatgagg aagctgctga tgaggtcttc
     6061 aaagacatca tggagtccag cccgggctcc agcccgccca acctgactcc aaaacccctc
     6121 cggcggcagg tcaccgtggc ccctgcctcg ggcctccccc acaaggaaga agctggaaag
     6181 ggcagtgcct tagggacccc tgctgcagct gagccagtga cccccaccag caaagcaggc
     6241 tcaggtgcac cagggggcac cagcaagggc cccgccgagg agtccagagt gaggaggcac
     6301 aagcactcct ctgagtcgcc agggagggac aaggggaaat tgtccaggct caaacctgcc
     6361 ccgccgcccc caccagcagc ctctgcaggg aaggctggag gaaagccctc gcagagcccg
     6421 agccaggagg cggccgggga ggcagtcctg ggcgcaaaga caaaagccac gagtctggtt
     6481 gatgctgtga acagtgacgc tgccaagccc agccagccgg gagagggcct caaaaagccc
     6541 gtgctcccgg ccactccaaa gccacagtcc gccaagccgt cggggacccc catcagccca
     6601 gcccccgttc cctccacgtt gccatcagca tcctcggccc tggcagggga ccagccgtct
     6661 tccaccgcct tcatccctct catatcaacc cgagtgtctc ttcggaaaac ccgccagcct
     6721 ccagagcgga tcgccagcgg cgccatcacc aagggcgtgg tcctggacag caccgaggcg
     6781 ctgtgcctcg ccatctctag gaactccgag cagatggcca gccacagcgc agtgctggag
     6841 gccggcaaaa acctctacac gttctgcgtg agctatgtgg attccatcca gcaaatgagg
     6901 aacaagtttg ccttccgaga ggccatcaac aaactggaga ataatctccg ggagcttcag
     6961 atctgcccgg cgacagcagg cagtggtcca gcggccactc aggacttcag caagctcctc
     7021 agttcggtga aggaaatcag tgacatagtg cagaggtagt ctagagggcc cgtttaaacc
     7081 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     7141 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     7201 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     7261 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     7321 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     7381 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     7441 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     7501 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     7561 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     7621 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     7681 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     7741 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     7801 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     7861 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     7921 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     7981 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8041 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     8101 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     8161 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     8221 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     8281 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     8341 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     8401 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     8461 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     8521 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     8581 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     8641 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     8701 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     8761 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     8821 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     8881 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     8941 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     9001 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     9061 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     9121 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     9181 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     9241 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     9301 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     9361 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     9421 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     9481 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     9541 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     9601 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     9661 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     9721 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     9781 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     9841 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     9901 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     9961 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    10021 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    10081 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    10141 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    10201 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    10261 acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    10321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    10381 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    10441 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    10501 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    10561 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    10621 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    10681 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    10741 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    10801 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    10861 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10921 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10981 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    11041 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    11101 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    11161 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    11221 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    11281 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    11341 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    11401 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    11461 tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//