| 质粒名称: | pDEST53-LRRK2-G2019S |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 25045 |
| 存储实验室: | Mark Cookson |
|
载体骨架基本信息 |
|
| 载体名称: | pN1pcDNA-DEST53 |
| 载体出品公司: | Invitrogen |
| 空载体大小: | 7767 bp |
| 完整质粒大小: | 13749 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | LRRK2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 7584 bp |
| 基因突变: | Glycine 2019 mutated to Serine |
| 标签/融合蛋白: | GFP (N terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | attR1 (not destroyed) |
| 3’克隆位点: | attR2 (not destroyed) |
| 5’测序引物 | CMV-F, GFP-F |
| 3’测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pDEST53-LRRK2-G2019S was a gift from Mark Cookson (Addgene plasmid # 25045 ; http://n2t.net/addgene:25045 ; RRID:Addgene_25045) |
| 参考文献部分: | Kinase activity is required for the toxic effects of mutant LRRK2/dardarin. Greggio E, Jain S, Kingsbury A, Bandopadhyay R, Lewis P, Kaganovich A, van der Brug MP, Beilina A, Blackinton J, Thomas KJ, Ahmad R, Miller DW, Kesavapany S, Singleton A, Lees A, Harvey RJ, Harvey K, Cookson MR. Neurobiol Dis. 2006 Aug . 23(2):329-41. 10.1016/j.nbd.2006.04.001 PubMed 16750377 |
买家导航
pDEST53-LRRK2-G2019S载体质粒基本信息
pDEST53-LRRK2-G2019S质粒图谱载体图谱和pDEST53-LRRK2-G2019S载体序列质粒序列多克隆位点信息
pDEST53-LRRK2-G2019S质粒载体简介
pDEST53-LRRK2-G2019S质粒序列载体序列
LOCUS pDEST53-LRRK2-G2019S 13749 bp DNA circular SYN 14-APR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13749)
AUTHORS Greggio E, Jain S, Kingsbury A, Bandopadhyay R, Lewis P, Kaganovich
A, van der Brug MP, Beilina A, Blackinton J, Thomas KJ, Ahmad R,
Miller DW, Kesavapany S, Singleton A, Lees A, Harvey RJ, Harvey K,
Cookson MR
TITLE Kinase activity is required for the toxic effects of mutant
LRRK2/dardarin.
JOURNAL Neurobiol Dis. 2006 Aug . 23(2):329-41.
PUBMED 16750377
REFERENCE 2 (bases 1 to 13749)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Neurobiol
Dis. 2006 Aug . 23(2):329-41."
COMMENT Sequence Label: pDEST53-LRRK2-G2019S
FEATURES Location/Qualifiers
source 1..13749
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind complement(66..90)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
primer_bind complement(122..139)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 128..352
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 398..826
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(485..504)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 695..716
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(835..855)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 840..1169
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 1020..1155
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 1082..1101
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 1236..2030
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(1290..1309)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 1900..1919
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 2204..2325
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2241..2260)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2295..2314
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(2374..2390)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2374..2390)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(2387..2409)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 2398..2414
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(2422..2428,2429..2446,2447..2452))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2467..2488
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(2605..2622)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2776..3364)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2856..2875)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(3535..4326,4327..4395))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4158..4177
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4396..4500)
/gene="bla"
/label=AmpR promoter
primer_bind complement(4575..4594)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 4766..5145
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 5146..5349
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 5300..5320
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 5394..5413
/label=T7
/note="T7 promoter, forward primer"
promoter 5394..5412
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS join(5436..5438,5439..5441,5442..6152)
/codon_start=1
/product="Cycle 3 GFP (Crameri et al., 1996)"
/label=Cycle 3 GFP
/note="mammalian codon-optimized"
/translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITHGMDELYK"
primer_bind complement(5469..5497)
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind 6092..6112
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
protein_bind 6174..6198
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
ORIGIN
1 tgagaaaaga attagctgaa aaaatgagac gaacatctgt tgagtaagag agaagggcga
61 attcgaccca gctttcttgt acaaagtggt gataattaat taagataaac ccgctgatca
121 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
181 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
241 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
301 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
361 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
421 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
481 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
541 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
601 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
661 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
721 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
781 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
841 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
901 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
961 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
1021 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
1081 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
1141 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
1201 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
1261 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
1321 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
1381 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
1441 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
1501 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
1561 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
1621 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
1681 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
1741 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
1801 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
1861 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
1921 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
1981 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
2041 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
2101 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
2161 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
2221 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
2281 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
2341 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
2401 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
2461 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
2521 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
2581 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
2641 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
2701 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
2761 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
2821 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
2881 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
2941 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
3001 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
3061 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
3121 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
3181 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
3241 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
3301 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
3361 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
3421 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
3481 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
3541 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
3601 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
3661 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
3721 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
3781 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
3841 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
3901 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
3961 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
4021 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
4081 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
4141 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
4201 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
4261 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
4321 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
4381 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
4441 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
4501 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
4561 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
4621 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
4681 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
4741 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
4801 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
4861 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
4921 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
4981 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
5041 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
5101 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
5161 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
5221 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
5281 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
5341 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
5401 actcactata gggagaccca agctggctag acaccatggc cagcaaagga gaagaacttt
5461 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
5521 ctgtcagtgg agagggtgaa ggtgatgcta catacggaaa gcttaccctt aaatttattt
5581 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc tcttatggtg
5641 ttcaatgctt ttcccgttat ccggatcata tgaaacggca tgactttttc aagagtgcca
5701 tgcccgaagg ttatgtacag gaacgcacta tatctttcaa agatgacggg aactacaaga
5761 cgcgtgctga agtcaagttt gaaggtgata cccttgttaa tcgtatcgag ttaaaaggta
5821 ttgattttaa agaagatgga aacattctcg gacacaaact cgagtacaac tataactcac
5881 acaatgtata catcacggca gacaaacaaa agaatggaat caaagctaac ttcaaaattc
5941 gccacaacat tgaagatgga tccgttcaac tagcagacca ttatcaacaa aatactccaa
6001 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcgacacaa tctgcccttt
6061 cgaaagatcc caacgaaaag cgtgaccaca tggtccttct tgagtttgta actgctgctg
6121 ggattacaca tggcatggat gagctctaca aaagcggttc cggaccggat caaacaagtt
6181 tgtacaaaaa agcaggctcc gaattcgccc ttatggctag tggcagctgt caggggtgcg
6241 aagaggacga ggaaactctg aagaagttga tagtcaggct gaacaatgtc caggaaggaa
6301 aacagataga aacgctggtc caaatcctgg aggatctgct ggtgttcacg tactccgagc
6361 acgcctccaa gttatttcaa ggcaaaaata tccatgtgcc tctgttgatc gtcttggact
6421 cctatatgag agtcgcgagt gtgcagcagg tgggttggtc acttctgtgc aaattaatag
6481 aagtctgtcc aggtacaatg caaagcttaa tgggacccca ggatgttgga aatgattggg
6541 aagtccttgg tgttcaccaa ttgattctta aaatgctaac agttcataat gccagtgtaa
6601 acttgtcagt gattggactg aagaccttag atctcctcct aacttcaggt aaaatcacct
6661 tgctgatatt ggatgaagaa agtgatattt tcatgttaat ttttgatgcc atgcactcat
6721 ttccagccaa tgatgaagtc cagaaacttg gatgcaaagc tttacatgtg ctgtttgaga
6781 gagtctcaga ggagcaactg actgaatttg ttgagaacaa agattatatg atattgttaa
6841 gtgcgttaac aaattttaaa gatgaagagg aaattgtgct tcatgtgctg cattgtttac
6901 attccctagc gattccttgc aataatgtgg aagtcctcat gagtggcaat gtcaggtgtt
6961 ataatattgt ggtggaagct atgaaagcat tccctatgag tgaaagaatt caagaagtga
7021 gttgctgttt gctccatagg cttacattag gtaatttttt caatatcctg gtattaaacg
7081 aagtccatga gtttgtggtg aaagctgtgc agcagtaccc agagaatgca gcattgcaga
7141 tctcagcgct cagctgtttg gccctcctca ctgagactat tttcttaaat caagatttag
7201 aggaaaagaa tgagaatcaa gagaatgatg atgaggggga agaagataaa ttgttttggc
7261 tggaagcctg ttacaaagca ttaacgtggc atagaaagaa caagcacgtg caggaggccg
7321 catgctgggc actaaataat ctccttatgt accaaaacag tttacatgag aagattggag
7381 atgaagatgg ccatttccca gctcataggg aagtgatgct ctccatgctg atgcattctt
7441 catcaaagga agttttccag gcatctgcga atgcattgtc aactctctta gaacaaaatg
7501 ttaatttcag aaaaatactg ttatcaaaag gaatacacct gaatgttttg gagttaatgc
7561 agaagcatat acattctccc gaagtggctg aaagtggctg taaaatgcta aatcatcttt
7621 ttgaaggaag caacacttcc ctggatataa tggcagcagt ggtccccaaa atactaacag
7681 ttatgaaacg tcatgagaca tcattaccag tgcagctgga ggcgcttcga gctattttac
7741 attttatagt gcctggcatg ccagaagaat ccagggagga tacagaattt catcataagc
7801 taaatatggt taaaaaacag tgtttcaaga atgatattca caaactggtc ctagcagctt
7861 tgaacaggtt cattggaaat cctgggattc agaaatgtgg attaaaagta atttcttcta
7921 ttgtacattt tcctgatgca ttagagatgt tatccctgga aggtgctatg gattcagtgc
7981 ttcacacact gcagatgtat ccagatgacc aagaaattca gtgtctgggt ttaagtctta
8041 taggatactt gattacaaag aagaatgtgt tcataggaac tggacatctg ctggcaaaaa
8101 ttctggtttc cagcttatac cgatttaagg atgttgctga aatacagact aaaggatttc
8161 agacaatctt agcaatcctc aaattgtcag catctttttc taagctgctg gtgcatcatt
8221 catttgactt agtaatattc catcaaatgt cttccaatat catggaacaa aaggatcaac
8281 agtttctaaa cctctgttgc aagtgttttg caaaagtagc tatggatgat tacttaaaaa
8341 atgtgatgct agagagagcg tgtgatcaga ataacagcat catggttgaa tgcttgcttc
8401 tattgggagc agatgccaat caagcaaagg agggatcttc tttaatttgt caggtatgtg
8461 agaaagagag cagtcccaaa ttggtggaac tcttactgaa tagtggatct cgtgaacaag
8521 atgtacgaaa agcgttgacg ataagcattg ggaaaggtga cagccagatc atcagcttgc
8581 tcttaaggag gctggccctg gatgttgcca acaatagcat ttgccttgga ggattttgta
8641 taggaaaagt tgaaccttct tggcttggtc ctttatttcc agataagact tctaatttaa
8701 ggaaacaaac aaatatagca tctacactag caagaatggt gatcagatat cagatgaaaa
8761 gtgctgtgga agaaggaaca gcctcaggca gcgatggaaa tttttctgaa gatgtgctgt
8821 ctaaatttga tgaatggacc tttattcctg actcttctat ggacagtgtg tttgctcaaa
8881 gtgatgacct ggatagtgaa ggaagtgaag gctcatttct tgtgaaaaag aaatctaatt
8941 caattagtgt aggagaattt taccgagatg ccgtattaca gcgttgctca ccaaatttgc
9001 aaagacattc caattccttg gggcccattt ttgatcatga agatttactg aagcgaaaaa
9061 gaaaaatatt atcttcagat gattcactca ggtcatcaaa acttcaatcc catatgaggc
9121 attcagacag catttcttct ctggcttctg agagagaata tattacatca ctagaccttt
9181 cagcaaatga actaagagat attgatgccc taagccagaa atgctgtata agtgttcatt
9241 tggagcatct tgaaaagctg gagcttcacc agaatgcact cacgagcttt ccacaacagc
9301 tatgtgaaac tctgaagagt ttgacacatt tggacttgca cagtaataaa tttacatcat
9361 ttccttctta tttgttgaaa atgagttgta ttgctaatct tgatgtctct cgaaatgaca
9421 ttggaccctc agtggtttta gatcctacag tgaaatgtcc aactctgaaa cagtttaacc
9481 tgtcatataa ccagctgtct tttgtacctg agaacctcac tgatgtggta gagaaactgg
9541 agcagctcat tttagaagga aataaaatat cagggatatg ctcccccttg agactgaagg
9601 aactgaagat tttaaacctt agtaagaacc acatttcatc cctatcagag aactttcttg
9661 aggcttgtcc taaagtggag agtttcagtg ccagaatgaa ttttcttgct gctatgcctt
9721 tcttgcctcc ttctatgaca atcctaaaat tatctcagaa caaattttcc tgtattccag
9781 aagcaatttt aaatcttcca cacttgcggt ctttagatat gagcagcaat gatattcagt
9841 acctaccagg tcccgcacac tggaaatctt tgaacttaag ggaactctta tttagccata
9901 atcagatcag catcttggac ttgagtgaaa aagcatattt atggtctaga gtagagaaac
9961 tgcatctttc tcacaataaa ctgaaagaga ttcctcctga gattggctgt cttgaaaatc
10021 tgacatctct ggatgtcagt tacaacttgg aactaagatc ctttcccaat gaaatgggga
10081 aattaagcaa aatatgggat cttcctttgg atgaactgca tcttaacttt gattttaaac
10141 atataggatg taaagccaaa gacatcataa ggtttcttca acagcgatta aaaaaggctg
10201 tgccttataa ccgaatgaaa cttatgattg tgggaaatac tgggagtggt aaaaccacct
10261 tattgcagca attaatgaaa accaagaaat cagatcttgg aatgcaaagt gccacagttg
10321 gcatagatgt gaaagactgg cctatccaaa taagagacaa aagaaagaga gatctcgtcc
10381 taaatgtgtg ggattttgca ggtcgtgagg aattctatag tactcatccc cattttatga
10441 cgcagcgagc attgtacctt gctgtctatg acctcagcaa gggacaggct gaagttgatg
10501 ccatgaagcc ttggctcttc aatataaagg ctcgcgcttc ttcttcccct gtgattctcg
10561 ttggcacaca tttggatgtt tctgatgaga agcaacgcaa agcctgcatg agtaaaatca
10621 ccaaggaact cctgaataag cgagggttcc ctgccatacg agattaccac tttgtgaatg
10681 ccaccgagga atctgatgct ttggcaaaac ttcggaaaac catcataaac gagagcctta
10741 atttcaagat ccgagatcag cttgttgttg gacagctgat tccagactgc tatgtagaac
10801 ttgaaaaaat cattttatcg gagcgtaaaa atgtgccaat tgaatttccc gtaattgacc
10861 ggaaacgatt attacaacta gtgagagaaa atcagctgca gttagatgaa aatgagcttc
10921 ctcacgcagt tcactttcta aatgaatcag gagtccttct tcattttcaa gacccagcac
10981 tgcagttaag tgacttgtac tttgtggaac ccaagtggct ttgtaaaatc atggcacaga
11041 ttttgacagt gaaagtggaa ggttgtccaa aacaccctaa gggaattatt tcgcgtagag
11101 atgtggaaaa atttctttca aaaaaaagga aatttccaaa gaactacatg tcacagtatt
11161 ttaagctcct agaaaaattc cagattgctt tgccaatagg agaagaatat ttgctggttc
11221 caagcagttt gtctgaccac aggcctgtga tagagcttcc ccattgtgag aactctgaaa
11281 ttatcatccg actatatgaa atgccttatt ttccaatggg attttggtca agattaatca
11341 atcgattact tgagatttca ccttacatgc tttcagggag agaacgagca cttcgcccaa
11401 acagaatgta ttggcgacaa ggcatttact taaattggtc tcctgaagct tattgtctgg
11461 taggatctga agtcttagac aatcatccag agagtttctt aaaaattaca gttccttctt
11521 gtagaaaagg ctgtattctt ttgggccaag ttgtggacca cattgattct ctcatggaag
11581 aatggtttcc tgggttgctg gagattgata tttgtggtga aggagaaact ctgttgaaga
11641 aatgggcatt atatagtttt aatgatggcg aagaacatca aaaaatctta cttgatgact
11701 tgatgaagaa agcagaggaa ggagatctct tagtaaatcc agatcaacca aggctcacca
11761 ttccaatatc tcagattgcc cctgacttga ttttggctga cctgcctaga aatattatgt
11821 tgaataatga tgagttggaa tttgaacaag ctccagagtt tctcctaggt gatggcagtt
11881 ttggatcagt ttaccgagca gcctatgaag gagaagaagt ggctgtgaag atttttaata
11941 aacatacatc actcaggctg ttaagacaag agcttgtggt gctttgccac ctccaccacc
12001 ccagtttgat atctttgctg gcagctggga ttcgtccccg gatgttggtg atggagttag
12061 cctccaaggg ttccttggat cgcctgcttc agcaggacaa agccagcctc actagaaccc
12121 tacagcacag gatcgcactc cacgtagctg atggtttgag atacctccac tcagccatga
12181 ttatataccg agacctgaaa ccccacaatg tgctgctttt cacactgtat cccaatgctg
12241 ccatcatcgc aaagattgct gactacagca ttgctcagta ctgctgtaga atggggataa
12301 aaacatcaga gggcacacca gggtttcgtg cacctgaagt tgccagagga aatgtcattt
12361 ataaccaaca ggctgatgtt tattcatttg gtttactact ctatgacatt ttgacaactg
12421 gaggtagaat agtagagggt ttgaagtttc caaatgagtt tgatgaatta gaaatacaag
12481 gaaaattacc tgatccagtt aaagaatatg gttgtgcccc atggcctatg gttgagaaat
12541 taattaaaca gtgtttgaaa gaaaatcctc aagaaaggcc tacttctgcc caggtctttg
12601 acattttgaa ttcagctgaa ttagtctgtc tgacgagacg cattttatta cctaaaaacg
12661 taattgttga atgcatggtt gctacacatc acaacagcag gaatgcaagc atttggctgg
12721 gctgtgggca caccgacaga ggacagctct catttcttga cttaaatact gaaggataca
12781 cttctgagga agttgctgat agtagaatat tgtgcttagc cttggtgcat cttcctgttg
12841 aaaaggaaag ctggattgtg tctgggacac agtctggtac tctcctggtc atcaataccg
12901 aagatgggaa aaagagacat accctagaaa agatgactga ttctgtcact tgtttgtatt
12961 gcaattcctt ttccaagcaa agcaaacaaa aaaattttct tttggttgga accgctgatg
13021 gcaagttagc aatttttgaa gataagactg ttaagcttaa aggagctgct cctttgaaga
13081 tactaaatat aggaaatgtc agtactccat tgatgtgttt gagtgaatcc acaaattcaa
13141 cggaaagaaa tgtaatgtgg ggaggatgtg gcacaaagat tttctccttt tctaatgatt
13201 tcaccattca gaaactcatt gagacaagaa caagccaact gttttcttat gcagctttca
13261 gtgattccaa catcataaca gtggtggtag acactgctct ctatattgct aagcaaaata
13321 gccctgttgt ggaagtgtgg gataagaaaa ctgaaaaact ctgtggacta atagactgcg
13381 tgcacttttt aagggaggta atggtaaaag aaaacaagga atcaaaacac aaaatgtctt
13441 attctgggag agtgaaaacc ctctgccttc agaagaacac tgctctttgg ataggaactg
13501 gaggaggcca tattttactc ctggatcttt caactcgtcg acttatacgt gtaatttaca
13561 acttttgtaa ttcggtcaga gtcatgatga cagcacagct aggaagcctt aaaaatgtca
13621 tgctggtatt gggctacaac cggaaaaata ctgaaggtac acaaaagcag aaagagatac
13681 aatcttgctt gaccgtttgg gacatcaatc ttccacatga agtgcaaaat ttagaaaaac
13741 acattgaag
//
