| 质粒名称: | pUDP082 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 103875 |
| 存储实验室: | Jean-Marc Daran |
|
载体骨架基本信息 |
|
| 载体名称: | pUDP002 |
| 载体出品公司: | Clontech |
| 空载体大小: | 10046 bp |
| 完整质粒大小: | 10237bp |
| 载体类型: | Yeast Expression, CRISPR |
| 筛选标记: | Hygromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: |
HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus
gRNA/shRNA
|
| 物种来源: | Kluyveromyces marxianus |
| 启动子: | ScTDH3 |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BsaI (destroyed during cloning) |
| 3’克隆位点: | BsaI (destroyed during cloning) |
| 5’测序引物 | AAGTATATAAAGACGGTAGG |
| 3’测序引物 | GATATCGACAAAGGAAAAGGGGCC |
|
文献引用方法 |
|
| 材料和方法部分: | pUDP082 was a gift from Jean-Marc Daran (Addgene plasmid # 103875 ; http://n2t.net/addgene:103875 ; RRID:Addgene_103875) |
| 参考文献部分: | Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA co-expression plasmid. Juergens H, Varela JA, Gorter de Vries AR, Perli T, Gast VJM, Gyurchev NY, Rajkumar AS, Mans R, Pronk JT, Morrissey JP, Daran JG. FEMS Yeast Res. 2018 May 1;18(3). pii: 4847887. doi: 10.1093/femsyr/foy012. 10.1093/femsyr/foy012 PubMed 29438517 |
买家导航
pUDP082载体质粒基本信息
pUDP082质粒图谱载体图谱和pUDP082载体序列质粒序列多克隆位点信息
pUDP082质粒载体简介
pUDP082质粒序列载体序列
LOCUS pUDP082 10237 bp DNA circular SYN 14-APR-2025
DEFINITION Broad-host-range Cas9/gRNA co-expression plasmid with gRNA
targetting ADE2 from Kluyveromyces marxianus.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10237)
AUTHORS Juergens H, Varela JA, Gorter de Vries AR, Perli T, Gast VJM,
Gyurchev NY, Rajkumar AS, Mans R, Pronk JT, Morrissey JP, Daran JG
TITLE Genome editing in Kluyveromyces and Ogataea yeasts using a
broad-host-range Cas9/gRNA co-expression plasmid.
JOURNAL FEMS Yeast Res. 2018 May 1;18(3). pii: 4847887. doi:
10.1093/femsyr/foy012.
PUBMED 29438517
REFERENCE 2 (bases 1 to 10237)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast
Res."; date: "2018-05-1"; volume: "18(3). pii: 4847887. doi"; pages:
" 10.1093/femsyr/foy012"
COMMENT Sequence Label: pUDP082
FEATURES Location/Qualifiers
source 1..10237
/mol_type="other DNA"
/organism="synthetic DNA construct"
gene 11..1588
/label=hphMX6
/note="yeast selectable marker conferring hygromycin
resistance"
CDS 359..1387
/codon_start=1
/product="hygromycin B phosphotransferase"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
KE"
CDS 359..1387
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
KE"
CDS 1966..1989
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 1993..2013
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 2014..6114
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTYKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSITHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 6118..6138
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
promoter 7057..7723
/gene="S. cerevisiae TDH3"
/label=GAP promoter
/note="promoter for glyceraldehyde-3-phosphate
dehydrogenase; also known as the TDH3 promoter"
primer_bind 7593..7616
/label=GPDpro-F
/note="S. cerevisiae GPD promoter, forward primer"
ncRNA 7730..7766
/product="self-cleaving hammerhead ribozyme (Ferre-D'Amare
and Scott, 2010)"
/label=hammerhead ribozyme
/ncRNA_class="hammerhead_ribozyme"
misc_RNA 7787..7862
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind complement(7976..7994)
/label=CYC1
/note="CYC1 transcription termination signal, reverse
primer"
CDS complement(join(8428..9219,9220..9288))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9051..9070
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9289..9393)
/gene="bla"
/label=AmpR promoter
rep_origin 9496..10084
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9985..10004
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 caataggcac gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc
61 aggggcatga tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt
121 tgcatccata cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctgca
181 gacctgcgag cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc
241 cctgtagaga aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc
301 ttttaaaatc ttgctaggat acagttctca catcacatcc gaacataaac aaggatccat
361 gggtaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
421 cagcgtgtcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
481 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
541 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
601 tggggaattt agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
661 gcaagacctg cctgaaaccg aactgcccgc tgttctgcaa ccggtcgcgg aggccatgga
721 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
781 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
841 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
901 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
961 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
1021 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
1081 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
1141 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
1201 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
1261 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
1321 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
1381 ggaataactc gaggacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt
1441 tttttatatt gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg
1501 cctcgacatc atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa
1561 tcgtatgtga atgctggtcg ctatactgga gctctcgaga accctcacct ttcgagagga
1621 cgatgcccgt gtctaaatga ttcgaccagc ctaagaatgt tcaacaatct ataatcagtc
1681 catagtcaac aagagccctc aaagttgata tcgtttagtg atctccctcg agatgagcgg
1741 aggatggttg ctttttgggg agccctaagt ggtacgtgcg gcgagcggga tgtttcccca
1801 ttaggcaaag gacgaccggg taacccctcg agaaaaaaaa tttttcgccg ctaatctggt
1861 gttatataaa gctccccctg ctcctggaat ttttttcctt gtcaactcac accggaaatc
1921 aaggcatttc attctgagta gttcttaaaa acataatcaa caatggatta taaagatgac
1981 gatgacaaac ctccaaaaaa gaagagaaag gtcgataaga aatactcaat aggcttagat
2041 atcggcacaa atagcgtcgg atgggcggtg atcactgatg aatataaggt tccgtctaaa
2101 aagttcaagg ttctgggaaa tacagaccgc cacagtatca aaaaaaatct tataggggct
2161 cttttatttg acagtggaga gacagcggaa gcgactcgtc tcaaacggac agctcgtaga
2221 aggtatacac gtcggaagaa tcgtatttgt tatctacagg agattttttc aaatgagatg
2281 gcgaaagtag atgatagttt ctttcatcga cttgaagagt cttttttggt ggaagaagac
2341 aagaagcatg aacgtcatcc tatttttgga aatatagtag atgaagttgc ttatcatgag
2401 aaatatccaa ctatctatca tctgcgaaaa aaattggtag attctactta taaagcggat
2461 ttgcgcttaa tctatttggc cttagcgcat atgattaagt ttcgtggtca ttttttgatt
2521 gagggagatt taaatcctga taatagtgat gtggacaaac tatttatcca gttggtacaa
2581 acctacaatc aattatttga agaaaaccct attaacgcaa gtggagtaga tgctaaagcg
2641 attctttctg cacgattgag taaatcaaga cgattagaaa atctcattgc tcagctcccc
2701 ggtgagaaga aaaatggctt atttgggaat ctcattgctt tgtcattggg tttgacccct
2761 aattttaaat caaattttga tttggcagaa gatgctaaat tacagctttc aaaagatact
2821 tacgatgatg atttagataa tttattggcg caaattggag atcaatatgc tgatttgttt
2881 ttggcagcta agaatttatc agatgctatt ttactttcag atatcctaag agtaaatact
2941 gaaataacta aggctcccct atcagcttca atgattaaac gctacgatga acatcatcaa
3001 gacttgactc ttttaaaagc tttagttcga caacaacttc cagaaaagta taaagaaatc
3061 ttttttgatc aatcaaaaaa cggatatgca ggttatattg atgggggagc tagccaagaa
3121 gaattttata aatttatcaa accaatttta gaaaaaatgg atggtactga ggaattattg
3181 gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga cctttgacaa cggctctatt
3241 acccatcaaa ttcacttggg tgagctgcat gctattttga gaagacaaga agacttttat
3301 ccatttttaa aagacaatcg tgagaagatt gaaaaaatct tgacttttcg aattccttat
3361 tatgttggtc cattggcgcg tggcaatagt cgttttgcat ggatgactcg gaagtctgaa
3421 gaaacaatta ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc agctcaatca
3481 tttattgaac gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt actaccaaaa
3541 catagtttgc tttatgagta ttttacggtt tataacgaat tgacaaaggt caaatatgtt
3601 actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc cattgttgat
3661 ttactcttca aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa
3721 aaaatagaat gttttgatag tgttgaaatt tcaggagttg aagatagatt taatgcttca
3781 ttaggtacct accatgattt gctaaaaatt attaaagata aagatttttt ggataatgaa
3841 gaaaatgaag atatcttaga ggatattgtt ttaacattga ccttatttga agatagggag
3901 atgattgagg aaagacttaa aacatatgct cacctctttg atgataaggt gatgaaacag
3961 cttaaacgtc gccgttatac tggttgggga cgtttgtctc gaaaattgat taatggtatt
4021 agggataagc aatctggcaa aacaatatta gattttttga aatcagatgg ttttgccaat
4081 cgcaatttta tgcagctgat ccatgatgat agtttgacat ttaaagaaga cattcaaaaa
4141 gcacaagtgt ctggacaagg cgatagttta catgaacata ttgcaaattt agctggtagc
4201 cctgctatta aaaaaggtat tttacagact gtaaaagttg ttgatgaatt ggtcaaagta
4261 atggggcggc ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact
4321 caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa
4381 ttaggaagtc agattcttaa agagcatcct gttgaaaata ctcaattgca aaatgaaaag
4441 ctctatctct attatctcca aaatggaaga gacatgtatg tggaccaaga attagatatt
4501 aatcgtttaa gtgattatga tgtcgatcac attgttccac aaagtttcct taaagacgat
4561 tcaatagaca ataaggtctt aacgcgttct gataaaaatc gtggtaaatc ggataacgtt
4621 ccaagtgaag aagtagtcaa aaagatgaaa aactattgga gacaacttct aaacgccaag
4681 ttaatcactc aacgtaagtt tgataattta acgaaagctg aacgtggagg tttgagtgaa
4741 cttgataaag ctggttttat caaacgccaa ttggttgaaa ctcgccaaat cactaagcat
4801 gtggcacaaa ttttggatag tcgcatgaat actaaatacg atgaaaatga taaacttatt
4861 cgagaggtta aagtgattac cttaaaatct aaattagttt ctgacttccg aaaagatttc
4921 caattctata aagtacgtga gattaacaat taccatcatg cccatgatgc gtatctaaat
4981 gccgtcgttg gaactgcttt gattaagaaa tatccaaaac ttgaatcgga gtttgtctat
5041 ggtgattata aagtttatga tgttcgtaaa atgattgcta agtctgagca agaaataggc
5101 aaagcaaccg caaaatattt cttttactct aatatcatga acttcttcaa aacagaaatt
5161 acacttgcaa atggagagat tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga
5221 gaaattgtct gggataaagg gcgagatttt gccacagtgc gcaaagtatt gtccatgccc
5281 caagtcaata ttgtcaagaa aacagaagta cagacaggcg gattctccaa ggagtcaatt
5341 ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa aagactggga tccaaaaaaa
5401 tatggtggtt ttgatagtcc aacggtagct tattcagtcc tagtggttgc taaggtggaa
5461 aaagggaaat cgaagaagtt aaaatccgtt aaagagttac tagggatcac aattatggaa
5521 agaagttcct ttgaaaaaaa tccgattgac tttttagaag ctaaaggata taaggaagtt
5581 aaaaaagact taatcattaa actacctaaa tatagtcttt ttgagttaga aaacggtcgt
5641 aaacggatgc tggctagtgc cggagaatta caaaaaggaa atgagctggc tctgccaagc
5701 aaatatgtga attttttata tttagctagt cattatgaaa agttgaaggg tagtccagaa
5761 gataacgaac aaaaacaatt gtttgtggag cagcataagc attatttaga tgagattatt
5821 gagcaaatca gtgaattttc taagcgtgtt attttagcag atgccaattt agataaagtt
5881 cttagtgcat ataacaaaca tagagacaaa ccaatacgtg aacaagcaga aaatattatt
5941 catttattta cgttgacgaa tcttggagct cccgctgctt ttaaatattt tgatacaaca
6001 attgatcgta aacgatatac gtctacaaaa gaagttttag atgccactct tatccatcaa
6061 tccatcactg gtctttatga aacacgcatt gatttgagtc agctaggagg tgaccctcca
6121 aaaaagaaga gaaaggtctg agcggttttg tataactaaa taatattgga aactaaatac
6181 gaatacccaa attttttatc taaattttgc cgaaagatta aaatctgcag agatatccga
6241 aacaggtaaa tggatgtttc aatccctgta gtcagtcagg aacccatatt atattacagt
6301 attagtcgcc gcttaggcac gcctttaatt agcaaaatca aaccttaagt gcatatgccg
6361 tataagggaa actcaaagaa ctggcatcgc aaaaatgaaa acatacgttg aaactacggc
6421 aaaggattgg tcagatcgct tcatacaggg aaagttcggc atcaacatct ttggataata
6481 tcagaatgag aaagaacaga tacgcagtac gttttttggt gagctctttg cacttcttta
6541 gttctttcca tcaatatcag ttttttaaac ttttaggact aaaagtgatg tttaacttca
6601 aaatgtttaa aattttgttc ttcccgacgt tcattaagaa tactaataca ctttaataat
6661 tattttaata ttttgttcta aataatgact tttaattaaa aaagataaaa tataaaaaca
6721 tcataataac tcaccagagg ttaagaacaa aaaaacaaat tagatatctg ctaatccaat
6781 atagttaaat caatctttcc ttggtataat gggtatatta catatatttc aaggaccgac
6841 actcctacca aatatctaaa atttaccata ttaacataac atgtatataa acgtcaaatc
6901 ataatcagca ctaacgtctc acggatcgta tatgccgtag cgacaatcta agaactatgc
6961 gaggacacgc tagggccaag gcaaaaagat tccttgatta cgtaagggag ttagaatcat
7021 tttgaataaa aaacacgctt tttcagttcg agtttatcat tatcaatact gccatttcaa
7081 agaatacgta aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattagc
7141 cttttaattc tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata
7201 taacatcgta ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc
7261 ccgcttttta agctggcatc cagaaaaaaa aagaatccca gcaccaaaat attgttttct
7321 tcaccaacca tcagttcata ggtccattct cttagcgcaa ctacagagaa caggggcaca
7381 aacaggcaaa aaacgggcac aacctcaatg gagtgatgca acctgcctgg agtaaatgat
7441 gacacaaggc aattgaccca cgcatgtatc tatctcattt tcttacacct tctattacct
7501 tctgctctct ctgatttgga aaaagctgaa aaaaaaggtt gaaaccagtt ccctgaaatt
7561 attcccctac ttgactaata agtatataaa gacggtaggt attgattgta attctgtaaa
7621 tctatttctt aaacttctta aattctactt ttatagttag tctttttttt agttttaaaa
7681 caccaagaac ttagtttcga ataaacacac ataaacaaac aaagtcaagc tgatgagtcc
7741 gtgaggacga aacgagtaag ctcgtcgccc atttttctgc gtataggttt tagagctaga
7801 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
7861 gcttttggtc ggcatggtcc cagcctcctc gctggcgccg gctgggcaac atgcttcggc
7921 atggcgaatg ggacacaggc cccttttcct ttgtcgatat catgtaatta gttatgtcac
7981 gcttacattc acgccctccc cccacatccg ctctaaccga aaaggaagga gttagacaac
8041 ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta
8101 tatttcaaat ttttcttttt tttctgtaca aacgcgtgta cgcatgtaac attatactga
8161 aaaccttgct tgagaaggtt ttgggacaag cttacgtctc acggatcgta tatgccgtag
8221 cgacaatcta agaactatgc gaggacacgc tagcagctgt attcacgtag acggataggt
8281 atagccagac atcagcagca tacttcggga accgtaggcc accgagcggc cgcgattatc
8341 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
8401 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
8461 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
8521 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcggg acccacgctc
8581 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
8641 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
8701 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
8761 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
8821 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
8881 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
8941 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
9001 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
9061 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
9121 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
9181 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
9241 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
9301 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
9361 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgt
9421 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
9481 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
9541 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
9601 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
9661 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
9721 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
9781 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
9841 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
9901 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
9961 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
10021 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
10081 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
10141 catgttcttt cctgcgttat cccctgattc tgtggataac cgtgcggact atatgtgaag
10201 gcatggctat ggcacggcag acattccgcc agatcat
//
