| 质粒名称: | EGFR L858R |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 11012 |
| 存储实验室: | Matthew Meyerson |
|
载体骨架基本信息 |
|
| 载体名称: | pBABE puro |
| 载体出品公司: | available at Addgene |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5169 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | EGFR L858R,epidermal growth factor receptor,ERBB |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 3639 bp |
| 基因突变: | L858R (tumor-derived) |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SnaBI（克隆过程中销毁） |
| 3' 克隆位点: | SnaBI（克隆过程中销毁） |
| 5' 测序引物 | pBABE5' |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | EGFR L858R was a gift from Matthew Meyerson (Addgene plasmid # 11012 ; http://n2t.net/addgene:11012 ; RRID:Addgene_11012) |
| 参考文献部分: | Oncogenic Transformation by Inhibitor-Sensitive and -Resistant EGFR Mutants. Greulich H, Chen TH, Feng W, Janne PA, Alvarez JV, Zappaterra M, Bulmer SE, Frank DA, Hahn WC, Sellers WR, Meyerson M. PLoS Med. 2005 Oct 4. 2(11):e313. 10.1371/journal.pmed.0020313 PubMed 16187797 |
买家导航
EGFR L858R载体 EGFRL858R质粒基本信息
EGFR L858R质粒图谱 EGFRL858R载体图谱和EGFR L858R载体序列 EGFRL858R质粒序列多克隆位点信息
EGFR L858R质粒 EGFRL858R载体简介
EGFR L858R质粒序列 EGFRL858R载体序列
LOCUS EGFR L858R 8725 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Retroviral construct for expressing human EGFR with L858R mutation
in human cells.
ACCESSION .
VERSION .
KEYWORDS EGFR L858R
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8725)
AUTHORS Greulich H, Chen TH, Feng W, Janne PA, Alvarez JV, Zappaterra M,
Bulmer SE, Frank DA, Hahn WC, Sellers WR, Meyerson M
TITLE Oncogenic Transformation by Inhibitor-Sensitive and -Resistant EGFR
Mutants.
JOURNAL PLoS Med. 2005 Oct 4. 2(11):e313.
PUBMED 16187797
REFERENCE 2 (bases 1 to 8725)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8725
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1..3633
/codon_start=1
/product="precursor of human epidermal growth factor
receptor"
/label=EGFR
/translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGRAKLLGAEEKEYHA
EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
primer_bind complement(3670..3690)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3675..4004
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3855..3990
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3917..3936
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4014..4613
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4014..4033)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 4510..4530
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 4834..5303
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(5433..5450)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5604..6192)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5684..5703)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6352..7212)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6975..6994
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7213..7317)
/gene="bla"
/label=AmpR promoter
LTR 7348..7817
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 7882..8081
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 8282..8698
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 8628..8650
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 8666..8682
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
ORIGIN
1 atgcgaccct ccgggacggc cggggcagcg ctcctggcgc tgctggctgc gctctgcccg
61 gcgagtcggg ctctggagga aaagaaagtt tgccaaggca cgagtaacaa gctcacgcag
121 ttgggcactt ttgaagatca ttttctcagc ctccagagga tgttcaataa ctgtgaggtg
181 gtccttggga atttggaaat tacctatgtg cagaggaatt atgatctttc cttcttaaag
241 accatccagg aggtggctgg ttatgtcctc attgccctca acacagtgga gcgaattcct
301 ttggaaaacc tgcagatcat cagaggaaat atgtactacg aaaattccta tgccttagca
361 gtcttatcta actatgatgc aaataaaacc ggactgaagg agctgcccat gagaaattta
421 caggaaatcc tgcatggcgc cgtgcggttc agcaacaacc ctgccctgtg caacgtggag
481 agcatccagt ggcgggacat agtcagcagt gactttctca gcaacatgtc gatggacttc
541 cagaaccacc tgggcagctg ccaaaagtgt gatccaagct gtcccaatgg gagctgctgg
601 ggtgcaggag aggagaactg ccagaaactg accaaaatca tctgtgccca gcagtgctcc
661 gggcgctgcc gtggcaagtc ccccagtgac tgctgccaca accagtgtgc tgcaggctgc
721 acaggccccc gggagagcga ctgcctggtc tgccgcaaat tccgagacga agccacgtgc
781 aaggacacct gccccccact catgctctac aaccccacca cgtaccagat ggatgtgaac
841 cccgagggca aatacagctt tggtgccacc tgcgtgaaga agtgtccccg taattatgtg
901 gtgacagatc acggctcgtg cgtccgagcc tgtggggccg acagctatga gatggaggaa
961 gacggcgtcc gcaagtgtaa gaagtgcgaa gggccttgcc gcaaagtgtg taacggaata
1021 ggtattggtg aatttaaaga ctcactctcc ataaatgcta cgaatattaa acacttcaaa
1081 aactgcacct ccatcagtgg cgatctccac atcctgccgg tggcatttag gggtgactcc
1141 ttcacacata ctcctcctct ggatccacag gaactggata ttctgaaaac cgtaaaggaa
1201 atcacagggt ttttgctgat tcaggcttgg cctgaaaaca ggacggacct ccatgccttt
1261 gagaacctag aaatcatacg cggcaggacc aagcaacatg gtcagttttc tcttgcagtc
1321 gtcagcctga acataacatc cttgggatta cgctccctca aggagataag tgatggagat
1381 gtgataattt caggaaacaa aaatttgtgc tatgcaaata caataaactg gaaaaaactg
1441 tttgggacct ccggtcagaa aaccaaaatt ataagcaaca gaggtgaaaa cagctgcaag
1501 gccacaggcc aggtctgcca tgccttgtgc tcccccgagg gctgctgggg cccggagccc
1561 agggactgcg tctcttgccg gaatgtcagc cgaggcaggg aatgcgtgga caagtgcaac
1621 cttctggagg gtgagccaag ggagtttgtg gagaactctg agtgcataca gtgccaccca
1681 gagtgcctgc ctcaggccat gaacatcacc tgcacaggac ggggaccaga caactgtatc
1741 cagtgtgccc actacattga cggcccccac tgcgtcaaga cctgcccggc aggagtcatg
1801 ggagaaaaca acaccctggt ctggaagtac gcagacgccg gccatgtgtg ccacctgtgc
1861 catccaaact gcacctacgg atgcactggg ccaggtcttg aaggctgtcc aacgaatggg
1921 cctaagatcc cgtccatcgc cactgggatg gtgggggccc tcctcttgct gctggtggtg
1981 gccctgggga tcggcctctt catgcgaagg cgccacatcg ttcggaagcg cacgctgcgg
2041 aggctgctgc aggagaggga gcttgtggag cctcttacac ccagtggaga agctcccaac
2101 caagctctct tgaggatctt gaaggaaact gaattcaaaa agatcaaagt gctgggctcc
2161 ggtgcgttcg gcacggtgta taagggactc tggatcccag aaggtgagaa agttaaaatt
2221 cccgtcgcta tcaaggaatt aagagaagca acatctccga aagccaacaa ggaaatcctc
2281 gatgaagcct acgtgatggc cagcgtggac aacccccacg tgtgccgcct gctgggcatc
2341 tgcctcacct ccaccgtgca actcatcacg cagctcatgc ccttcggctg cctcctggac
2401 tatgtccggg aacacaaaga caatattggc tcccagtacc tgctcaactg gtgtgtgcag
2461 atcgcaaagg gcatgaacta cttggaggac cgtcgcttgg tgcaccgcga cctggcagcc
2521 aggaacgtac tggtgaaaac accgcagcat gtcaagatca cagattttgg gagggccaaa
2581 ctgctgggtg cggaagagaa agaataccat gcagaaggag gcaaagtgcc tatcaagtgg
2641 atggcattgg aatcaatttt acacagaatc tatacccacc agagtgatgt ctggagctac
2701 ggggtgaccg tttgggagtt gatgaccttt ggatccaagc catatgacgg aatccctgcc
2761 agcgagatct cctccatcct ggagaaagga gaacgcctcc ctcagccacc catatgtacc
2821 atcgatgtct acatgatcat ggtcaagtgc tggatgatag acgcagatag tcgcccaaag
2881 ttccgtgagt tgatcatcga attctccaaa atggcccgag acccccagcg ctaccttgtc
2941 attcaggggg atgaaagaat gcatttgcca agtcctacag actccaactt ctaccgtgcc
3001 ctgatggatg aagaagacat ggacgacgtg gtggatgccg acgagtacct catcccacag
3061 cagggcttct tcagcagccc ctccacgtca cggactcccc tcctgagctc tctgagtgca
3121 accagcaaca attccaccgt ggcttgcatt gatagaaatg ggctgcaaag ctgtcccatc
3181 aaggaagaca gcttcttgca gcgatacagc tcagacccca caggcgcctt gactgaggac
3241 agcatagacg acaccttcct cccagtgcct gaatacataa accagtccgt tcccaaaagg
3301 cccgctggct ctgtgcagaa tcctgtctat cacaatcagc ctctgaaccc cgcgcccagc
3361 agagacccac actaccagga cccccacagc actgcagtgg gcaaccccga gtatctcaac
3421 actgtccagc ccacctgtgt caacagcaca ttcgacagcc ctgcccactg ggcccagaaa
3481 ggcagccacc aaattagcct ggacaaccct gactaccagc aggacttctt tcccaaggaa
3541 gccaagccaa atggcatctt taagggctcc acagctgaaa atgcagaata cctaagggtc
3601 gcgccacaaa gcagtgaatt tattggagca tgagatgtag gaattcgcca gcacagtggt
3661 cgaccctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
3721 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
3781 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
3841 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
3901 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
3961 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt accatgaccg
4021 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
4081 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
4141 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
4201 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
4261 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
4321 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
4381 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
4441 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
4501 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
4561 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
4621 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg ataaaataaa
4681 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
4741 agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga
4801 agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg
4861 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc
4921 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc
4981 cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc
5041 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
5101 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg
5161 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
5221 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
5281 tacccgtcag cgggggtctt tcacatgcag catgtatcaa aattaatttg gttttttttc
5341 ttaagtattt acattaaatg gccatagttg cattaatgaa tcggccaacg cgcggggaga
5401 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
5461 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
5521 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
5581 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
5641 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
5701 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
5761 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
5821 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
5881 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
5941 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
6001 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
6061 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
6121 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
6181 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
6241 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
6301 ttgcggccgc aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
6361 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
6421 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
6481 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
6541 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
6601 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
6661 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
6721 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
6781 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
6841 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
6901 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
6961 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
7021 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
7081 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
7141 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
7201 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
7261 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
7321 atttccccga aaagtgccac ctgcagcctg aatatgggcc aaacaggata tctgtggtaa
7381 gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca
7441 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
7501 tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
7561 acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt
7621 cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact cggggcgcca
7681 gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg
7741 catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg
7801 tcagcggggg tctttcattt gggggctcgt ccgggatcgg gagacccctg cccagggacc
7861 accgacccac caccgggagg caagctggcc agcaacttat ctgtgtctgt ccgattgtct
7921 agtgtctatg actgatttta tgcgcctgcg tcggtactag ttagctaact agctctgtat
7981 ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg
8041 tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt cgatgtggaa
8101 tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac agttcccgcc
8161 tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg
8221 ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta
8281 gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag atgtcgagcg
8341 gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct tctgctctgc
8401 agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc gagacctcat
8461 cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag accaggtccc
8521 ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca agccctttgt
8581 acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc ttgaacctcc
8641 tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc taggcgccgg
8701 ccggatccca gtgtggtggt acatc
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EGFR L858R质粒 EGFRL858R载体应用举例
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