| 质粒名称: | pCS2-nCas9n |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 47929 |
| 存储实验室: | Wenbiao Chen |
|
载体骨架基本信息 |
|
| 载体名称: | pCS2+ |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4100 bp |
| 完整质粒大小: | 8300 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达,CRISPR |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | nls-zcas9-nls |
| 物种来源: | Synthetic |
| 插入大小: | 4200 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | XbaI(未破坏) |
| 5' 测序引物 | SP6 |
| 3' 测序引物 | M13 Rev |
|
文献引用方法 |
|
| 材料和方法部分: | pCS2-nCas9n was a gift from Wenbiao Chen (Addgene plasmid # 47929 ; http://n2t.net/addgene:47929 ; RRID:Addgene_47929) |
| 参考文献部分: | Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Jao LE, Wente SR, Chen W. Proc Natl Acad Sci U S A. 2013 Aug 5. 10.1073/pnas.1308335110 PubMed 23918387 |
买家导航
pCS2-nCas9n载体 pCS2nCas9n质粒基本信息
pCS2-nCas9n质粒图谱 pCS2nCas9n载体图谱和pCS2-nCas9n载体序列 pCS2nCas9n质粒序列多克隆位点信息
pCS2-nCas9n质粒 pCS2nCas9n载体简介
pCS2-nCas9n质粒序列 pCS2nCas9n载体序列
LOCUS pCS2-nCas9n 8269 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION expression of an optimized Cas9 for genome-editing in zebrafish.
ACCESSION .
VERSION .
KEYWORDS pCS2-nCas9n
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8269)
AUTHORS Jao LE, Wente SR, Chen W
TITLE Efficient multiplex biallelic zebrafish genome editing using a
CRISPR nuclease system.
JOURNAL Proc Natl Acad Sci U S A. 2013 Aug 5.
PUBMED 23918387
REFERENCE 2 (bases 1 to 8269)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8269
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 30..1007
/label=CMV IE94 promoter
/note="enhancer/promoter region of simian cytomegalovirus
major immediate early transcription unit IE94"
promoter 1042..1060
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 1042..1059
/label=SP6
/note="SP6 promoter, forward primer"
regulatory 1106..1115
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1124..1144
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1151..5251
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes MGAS15252 Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKK
NLIGALLFGSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLADSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQIYNQLFEENPINASRVDAKAILSARLSKSRRL
ENLIAQLPGEKRNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGAYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDRGMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGHSLHEQIANLAGSPAIKKGILQTVKIVDELVKVMGH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
RBS 5164..5172
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 5267..5287
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
promoter complement(5306..5323)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 5328..5462
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5352..5371)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 5406..5425
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(5575..5595)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(5575..5593)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(5614..5630)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5614..5630)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5627..5649)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5638..5654
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5662..5692)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5707..5728
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5845..5862)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6016..6604)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6096..6115)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6775..7635)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7398..7417
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7636..7740)
/gene="bla"
/label=AmpR promoter
rep_origin complement(7766..8221)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7903..7924)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 8115..8134
/label=F1ori-R
/note="F1 origin, reverse primer"
ORIGIN
1 gggcctcttc gctattacgc cagtcgaccg ccaattcaat atggcgtata tggactcatg
61 ccaattcaat atggtggatc tggacctgtg ccaattcaat atggcgtata tggactcgtg
121 ccaattcaat atggtggatc tggaccccag ccaattcaat atggcggact tggcaccatg
181 ccaattcaat atggcggacc tggcactgtg ccaactgggg aggggtctac ttggcacggt
241 gccaagtttg aggaggggtc ttggccctgt gccaagtccg ccatattgaa ttggcatggt
301 gccaataatg gcggccatat tggctatatg ccaggatcaa tatataggca atatccaata
361 tggccctatg ccaatatggc tattggccag gttcaatact atgtattggc cctatgccat
421 atagtattcc atatatgggt tttcctattg acgtagatag cccctcccaa tgggcggtcc
481 catataccat atatggggct tcctaatacc gcccatagcc actcccccat tgacgtcaat
541 ggtctctata tatggtcttt cctattgacg tcatatgggc ggtcctattg acgtatatgg
601 cgcctccccc attgacgtca attacggtaa atggcccgcc tggctcaatg cccattgacg
661 tcaataggac cacccaccat tgacgtcaat gggatggctc attgcccatt catatccgtt
721 ctcacgcccc ctattgacgt caatgacggt aaatggccca cttggcagta catcaatatc
781 tattaatagt aacttggcaa gtacattact attggaagta cgccagggta cattggcagt
841 actcccattg acgtcaatgg cggtaaatgg cccgcgatgg ctgccaagta catccccatt
901 gacgtcaatg gggaggggca atgacgcaaa tgggcgttcc attgacgtaa atgggcggta
961 ggcgtgccta atgggaggtc tatataagca atgctcgttt agggaaccgc cattctgcct
1021 ggggacgtcg gagcaagctt gatttaggtg acactataga atacaagcta cttgttcttt
1081 ttgcaggatc cgatccaatg tcgacgccac catggcttct ccacctaaga agaagagaaa
1141 ggtgggaagc atggataaga agtatagcat cggcctggat attggaacta actccgtggg
1201 ttgggcagtg attacagacg actacaaggt ccctagcaag aaatttaagg tgctgggtaa
1261 caccgacagg cacagcatca agaaaaatct gattggagcc ctgctgttcg gttctggaga
1321 gactgccgaa gcaacacgcc tgaaaagaac agcaagaagg cgctatacca gaaggaagaa
1381 tagaatctgt tacctgcagg agattttctc taacgaaatg gctaaggtgg acgattcatt
1441 ctttcatagg ctggaggaaa gtttcctggt cgaggaagat aagaaacacg agcgccatcc
1501 tatctttgga aacattgtgg acgaggtcgc ctatcacgaa aaatacccaa ccatctatca
1561 tctgcgcaag aaactggctg actctactga taaagccgac ctgagactga tctatctggc
1621 tctggcccac atgattaagt tcaggggtca ttttctgatc gagggcgatc tgaaccccga
1681 caattccgat gtggacaagc tgttcatcca gctggtccag atttacaatc agctgtttga
1741 ggaaaaccct attaatgctt ccagagtgga cgcaaaagct atcctgtcag ccaggctgtc
1801 caagtcacgc agactggaga acctgattgc acagctgccc ggagaaaaga ggaacggtct
1861 gtttggaaat ctgatcgctc tgagtctggg cctgactcct aacttcaaaa gcaattttga
1921 tctggctgag gacgccaaac tgcagctgtc aaaggacaca tatgacgatg acctggataa
1981 cctgctggca cagatcggag atcagtacgc tgacctgttc ctggctgcca aaaatctgtc
2041 cgacgcaatc ctgctgtcag atattctgag agtgaacagc gagattacaa aagctcctct
2101 gagtgccagc atgatcaaga gatatgacga gcaccatcag gatctgaccc tgctgaaggc
2161 tctggtcagg cagcagctgc cagagaagta caaggaaatt ttctttgatc agtccaagaa
2221 cggctacgcc ggttatatcg acggaggcgc atcacaggag gaattctaca agtttatcaa
2281 acctattctg gagaagatgg acggaactga ggaactgctg gtgaaactga atagagagga
2341 cctgctgagg aagcagcgca catttgataa cggttccatc ccacaccaga ttcatctggg
2401 agagctgcac gctatcctga ggcgccagga agacttctac ccctttctga aagataaccg
2461 cgagaagatc gaaaaaattc tgaccttcag aatcccttac tatgtgggtc cactggctcg
2521 cggaaacagc agatttgcct ggatgactcg caaatccgag gaaaccatta ctccttggaa
2581 cttcgaggaa gtggtcgata agggcgcctc tgcacagtcc ttcatcgaga gaatgactaa
2641 ttttgacaaa aacctgccca atgagaaagt gctgcctaag cactccctgc tgtacgagta
2701 tttcactgtc tataacgaac tgacaaaggt gaaatacgtc accgagggca tgagaaagcc
2761 agccttcctg tcaggagagc agaagaaagc aatcgtggat ctgctgttta aaaccaacag
2821 gaaagtgact gtcaagcagc tgaaggagga ctacttcaag aaaattgaat gcttcgattc
2881 cgtggagatc agcggagtcg aagacagatt taacgcaagc ctgggcgctt accacgatct
2941 gctgaagatc attaaggata aagacttcct ggacaacgag gaaaatgagg atatcctgga
3001 agacattgtg ctgacactga ccctgtttga ggacagagga atgatcgagg aaagactgaa
3061 aacctatgct catctgttcg atgacaaggt gatgaaacag ctgaagagaa ggcgctacac
3121 tggctggggt agactgagca ggaagctgat caacggcatt agggataaac agtcaggaaa
3181 gacaatcctg gactttctga aaagtgatgg cttcgccaac cgcaatttta tgcagctgat
3241 tcacgatgac agtctgacct tcaaagagga catccagaag gctcaggtgt ctggacaggg
3301 ccactccctg catgagcaga ttgcaaacct ggctggaagc ccagccatca agaaaggcat
3361 tctgcagaca gtgaaaatcg tcgatgagct ggtgaaagtc atgggccata agcccgaaaa
3421 catcgtgatt gagatggctc gcgaaaatca gacaacccag aagggtcaga agaacagtag
3481 agagaggatg aaaagaatcg aggaaggcat taaggagctg ggtagccaga tcctgaaaga
3541 gcacccagtg gaaaacacac agctgcagaa tgagaagctg tatctgtact atctgcagaa
3601 tggaagagat atgtacgtgg accaggagct ggatattaac aggctgtctg attacgacgt
3661 ggatcatatc gtcccccaga gtttcatcaa agatgacagc attgacaaca aggtgctgac
3721 caggtccgac aaaaacagag gaaaatcaga taatgtccct agtgaggaag tggtcaagaa
3781 aatgaagaac tactggagac agctgctgaa tgccaaactg atcactcaga ggaagtttga
3841 taacctgaca aaagcagagc gcggtggact gtcagaactg gacaaagctg gattcatcaa
3901 gaggcagctg gtggaaacac gccagatcac taaacacgtc gcacagattc tggatagtcg
3961 catgaacaca aagtacgatg agaatgacaa actgatcaga gaagtgaagg tcattaccct
4021 gaagagtaaa ctggtcagcg actttaggaa agatttccag ttttataagg tccgcgagat
4081 taacaattat caccatgccc atgacgcata cctgaacgcc gtggtcggta ccgcactgat
4141 caagaaatac ccaaaactgg agagcgaatt cgtgtacgga gactataagg tgtacgatgt
4201 cagaaaaatg atcgccaagt ccgagcagga aattggaaaa gctactgcca agtatttctt
4261 ttactcaaac atcatgaatt tctttaagac agagatcacc ctggccaatg gagaaatccg
4321 caaaaggccc ctgattgaga caaacggaga gacaggcgaa atcgtgtggg acaaaggcag
4381 agattttgca accgtgagga aggtcctgag catgcctcaa gtgaatatcg tcaagaaaac
4441 tgaggtgcag acaggcggtt tctcaaaaga aagtattctg ccaaaacgca actctgataa
4501 gctgatcgct agaaagaaag actgggaccc taagaagtat ggaggctttg actctcccac
4561 tgtggcatac tccgtcctgg tggtcgctaa ggtggagaag ggcaaaagca agaaactgaa
4621 atctgtcaag gagctgctgg gtatcacaat tatggagaga agctctttcg agaagaaccc
4681 aatcgatttt ctggaggcca aaggttataa ggaagtgaag aaagacctga tcattaaact
4741 gcccaagtac agtctgtttg agctggaaaa cggcaggaaa cgcatgctgg caagcgctgg
4801 agagctgcag aaaggcaatg aactggccct gccttctaag tacgtgaact tcctgtatct
4861 ggcaagccac tacgagaagc tgaaaggatc tccagaggat aacgaacaga aacagctgtt
4921 tgtggagcag cacaagcatt atctggacga gatcattgaa cagattagcg agttctctaa
4981 aagagtgatc ctggccgacg caaatctgga taaggtcctg tctgcttaca acaaacacag
5041 agataagccc atcagggagc aggccgaaaa tatcattcat ctgttcactc tgacaaacct
5101 gggcgcacct gcagctttca agtacttcga cactacaatc gatagaaaga ggtacacctc
5161 cactaaggag gtgctggacg ctacactgat ccatcagagt attaccggcc tgtacgaaac
5221 aaggattgac ctgtctcagc tgggtggcga ctcaccggtg agatctccta agaagaagag
5281 aaaggtgtga ccgcggaatc tagaactata gtgagtcgta ttacgtagat ccagacatga
5341 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
5401 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
5461 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
5521 tttaattcgc ggccgcggcg ccaatgcatt gggcccggta cccagctttt gttcccttta
5581 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
5641 ttatccgctc acaattccac acaacatacg agccgggagc ataaagtgta aagcctgggg
5701 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
5761 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
5821 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
5881 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
5941 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
6001 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
6061 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
6121 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
6181 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
6241 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
6301 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
6361 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
6421 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
6481 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
6541 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
6601 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
6661 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
6721 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
6781 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
6841 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
6901 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
6961 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
7021 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
7081 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
7141 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
7201 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
7261 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
7321 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
7381 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
7441 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
7501 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
7561 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
7621 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
7681 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
7741 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
7801 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
7861 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
7921 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
7981 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
8041 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
8101 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
8161 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
8221 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgc
//
