pMAL-c5x-UBAP2L

LOCUS       pMAL-c5x-UBAP2L                8982 bp DNA     circular SYN 04-MAR-2025
DEFINITION  TSN20250106-0571-00015_949_20250107-BAN39_B02_B02_i60.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 17, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(959..1150)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        1646..1723
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             1724..2806
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        3049..3077
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    3085..3101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3171..4271
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     CDS             4320..4331
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4371..4391
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             4401..7661
                     /codon_start=1
                     /label=UBAP2L (NM_001375619.1)
                     /translation="MMTSVGTNRARGNWEQPQNQNQTQHKQRPQATAEQIRLAQMISDH
                     NDADFEEKVKQLIDITGKNQDECVIALHDCNGDVNRAINVLLEGNPDTHSWEMVGKKKG
                     VSGQKDGGQTESNEEGKENRDRDRDYSRRRGGPPRRGRGASRGREFRGQENGLDGTKSG
                     GPSGRGTERGRRGRGRGRGGSGRRGGRFSAQGMGTFNPADYAEPANTDDNYGNSSGNTW
                     NNTGHFEPDDGTSAWRTATEEWGTEDWNEDLSETKIFTASNVSSVPLPAENVTITAGQR
                     IDLAVLLGKTPSTMENDSSNLDPSQAPSLAQPLVFSNSKQTAISQPASGNTFSHHSMVS
                     MLGKGFGDVGEAKGGSTTGSQFLEQFKTAQALAQLAAQHSQSGSTTTSSWDMGSTTQSP
                     SLVQYDLKNPSDSAVHSPFTKRQAFTPSSTMMEVFLQEKSPAVATSTAAPPPPSSPLPS
                     KSTSAPQMSPGSSDNQSSSPQPAQQKLKQQKKKASLTSKIPALAVEMPGSADISGLNLQ
                     FGALQFGSEPVLSDYESTPTTSASSSQAPSSLYTSTASESSSTISSNQSQESGYQSGPI
                     QSTTYTSQNNAQGPLYEQRSTQTRRYPSSISSSPQKDLTQAKNGFSSVQATQLQTTQSV
                     EGATGSAVKSDSPSTSSIPPLNETVSAASLLTTTNQHSSSLGGLSHSEEIPNTTTTQHS
                     STLSTQQNTLSSSTSSGRTSTSTLLHTSVESEANLHSSSSTFSTTSSTVSAPPPVVSVS
                     SSLNSGSSLGLSLGSNSTVTASTRSSVATTSGKAPPNLPPGVPPLLPNPYIMAPGLLHA
                     YPPQVYGYDDLQMLQTRFPLDYYSIPFPTPTTPLTGRDGSLASNPYSGDLTKFGRGDAS
                     SPAPATTLAQPQQNQTQTHHTTQQTFLNPALPPGYSYTSLPYYTGVPGLPSTFQYGPAV
                     FPVAPTSSKQHGVNVSVNASATPFQQPSGYGSHGYNTGVSVTSSNTGVPDISGSVYSKT
                     QQSFEKQGFHSGTPAASFNLPSALGSGGPINPATAAAYPPAPFMHILTPHQQPHSQILH
                     HHLQQDGQTGSGQRSQTSSIPQKPQTNKSAYNSYSWGAN"
     CDS             7668..7685
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      7711..7797
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7889..7916
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        7944..8034
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8035..8895
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatataag gtgcactgtg
      841 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
      901 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
      961 agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt
     1021 ggtcgtgcag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct
     1081 ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct
     1141 gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga
     1201 tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg
     1261 aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc
     1321 agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc
     1381 atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac
     1441 tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc
     1501 agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc
     1561 aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc
     1621 aggacccaac gctgcccgaa attccgacac catcgaatgg tgcaaaacct ttcgcggtat
     1681 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt
     1741 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
     1801 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa
     1861 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt
     1921 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
     1981 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
     2041 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
     2101 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
     2161 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
     2221 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
     2281 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
     2341 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
     2401 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
     2461 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
     2521 ggatatttcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt
     2581 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
     2641 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
     2701 aaaaaccacc ctggcgccca atacacaaac cgcctctccc cgcgcgttgg ccgattcatt
     2761 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2821 atgtaagtta gctcactcat taggcacaat tctcatgttt gacagcttat catcgactgc
     2881 acggtgcacc aatgcttctg gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag
     2941 gtcgtaaatc actgcataat tcgtgtcgct caaggcgcac tcccgttctg gataatgttt
     3001 tttgcgccga catcataacg gttctggcaa atattctgaa atgagctgtt gacaattaat
     3061 catcggctcg tataatgtgt ggaattgtga gcggataaca atttcacaca ggaaacagcc
     3121 agtccgttta ggtgttttca cgagcaattg accaacaagg accatagatt atgaaaatcg
     3181 aagaaggtaa actggtaatc tggattaacg gcgataaagg ctataacggt ctcgctgaag
     3241 tcggtaagaa attcgagaaa gataccggaa ttaaagtcac cgttgagcat ccggataaac
     3301 tggaagagaa attcccacag gttgcggcaa ctggcgatgg ccctgacatt atcttctggg
     3361 cacacgaccg ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc accccggaca
     3421 aagcgttcca ggacaagctg tatccgttta cctgggatgc cgtacgttac aacggcaagc
     3481 tgattgctta cccgatcgct gttgaagcgt tatcgctgat ttataacaaa gatctgctgc
     3541 cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga taaagaactg aaagcgaaag
     3601 gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg ctgattgctg
     3661 ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgacattaaa gacgtgggcg
     3721 tggataacgc tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt aaaaacaaac
     3781 acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa ggcgaaacag
     3841 cgatgaccat caacggcccg tgggcatggt ccaacatcga caccagcaaa gtgaattatg
     3901 gtgtaacggt actgccgacc ttcaagggtc aaccatccaa accgttcgtt ggcgtgctga
     3961 gcgcaggtat taacgccgcc agtccgaaca aagagctggc aaaagagttc ctcgaaaact
     4021 atctgctgac tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg ggtgccgtag
     4081 cgctgaagtc ttacgaggaa gagttggtga aagatccgcg tattgccgcc actatggaaa
     4141 acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc tggtatgccg
     4201 tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa gccctgaaag
     4261 acgcgcagac taattcgagc tcgaacaaca acaacaataa caataacaac aacctcggga
     4321 tcgagggaag gatttcacat atgtccatgg gcggccgcga tatcgtcgac gaaaacctgt
     4381 attttcagtc ggatggatcc atgatgacat cggtgggcac taaccgagcc cggggaaact
     4441 gggaacaacc tcaaaaccaa aaccagacac agcacaagca gcggccacag gccactgcag
     4501 aacaaattag acttgcacag atgatttcgg accataatga tgctgacttt gaggagaagg
     4561 tgaaacaatt gattgatatt acaggcaaga accaggatga atgtgtgatt gctttgcatg
     4621 actgcaatgg agatgtcaac agagctatca atgttcttct ggaaggaaac ccagacacgc
     4681 attcctggga gatggtcggg aagaagaagg gagtctcagg ccagaaggat ggtggccaga
     4741 cggaatccaa tgaggaaggc aaagaaaatc gagaccggga cagagactat agtcggcgac
     4801 gtggtgggcc accaagacgg gggagaggtg ccagccgtgg acgagagttt cgaggtcagg
     4861 aaaatggatt ggatggcacc aagagtggag ggccttctgg aagaggaaca gaaagaggca
     4921 gaaggggccg tggccgaggc agaggtggct ctggtaggcg aggaggaagg ttttctgctc
     4981 aaggaatggg aacctttaac ccagctgatt atgcagagcc agccaatact gatgataact
     5041 atggcaatag cagcggcaat acgtggaaca acactggcca ctttgaacca gatgatggga
     5101 cgagtgcatg gaggactgca acagaggagt gggggactga agattggaat gaagatcttt
     5161 ctgagaccaa gatcttcact gcctctaatg tgtcttcagt gcctctgcct gcggagaatg
     5221 tgacaatcac tgctggtcag agaattgacc ttgctgttct gctggggaag acaccatcta
     5281 caatggagaa tgattcatct aatctggatc cgtctcaggc tccttctctg gcccagcctc
     5341 tggtgttcag taattcgaag cagactgcca tatcacagcc tgcttcaggg aacacatttt
     5401 ctcatcacag tatggtgagc atgttaggga aaggatttgg tgatgtcggt gaagctaaag
     5461 gcggcagtac tacaggctcc cagttcttgg agcaattcaa gactgcccaa gccctggctc
     5521 agttggcagc tcagcattct cagtctggaa gcaccaccac ctcctcttgg gacatgggct
     5581 cgacgacaca atccccatca ctggtgcagt atgatttgaa gaacccaagt gattcagcag
     5641 tgcacagccc ctttacaaag cgccaggctt ttaccccatc ttcaaccatg atggaggtgt
     5701 tccttcagga gaagtcacct gcagtggcta cctccacagc tgcacctcca cctccgtctt
     5761 ctcctctgcc aagcaaatcc acatcggctc cacagatgtc gcctggatct tcagacaacc
     5821 agtcctctag ccctcagccg gctcagcaga aactgaaaca gcagaagaaa aaagcctcct
     5881 tgacttctaa gattcctgct ctggctgtgg agatgcctgg ctcagcagat atctcagggc
     5941 taaacctgca gtttggggca ttgcagtttg ggtcagagcc tgtcctttct gattatgagt
     6001 ccacccccac cacgagcgcc tcttcaagcc aggctccaag tagcctgtat accagcacgg
     6061 ccagtgaatc atcctctaca atttcatcta accagagtca ggagtctggt tatcagagcg
     6121 gcccaattca gtcgacaacc tatacctccc aaaataatgc tcagggccct ctttatgaac
     6181 agagatccac acagactcgg cggtacccca gctccatctc ttcatcaccc caaaaggacc
     6241 tgactcaggc aaagaatggc ttcagttctg tgcaggccac gcagttacag accacacaat
     6301 ctgttgaagg tgctacaggc tctgcagtga aatctgattc accttccact tctagcatcc
     6361 cccctctcaa tgaaacggta tctgcagctt ccttactgac gacaaccaat cagcattcat
     6421 cctccttggg tggcttgagc cacagtgagg agattccaaa tactaccacc acacaacaca
     6481 gcagcacgtt atctacgcag cagaataccc tttcatcatc aacatcttct gggcgcactt
     6541 cgacatccac tcttttgcac acaagtgtgg agagtgaggc gaatctccat tcttcctcca
     6601 gcactttttc caccacatcc agcacagtct ctgcacctcc cccagtggtc agtgtctcct
     6661 ccagtctcaa tagtggcagt agcctgggcc tcagcctagg cagcaactcc actgtcacag
     6721 cctcgactcg aagctcagtt gctacgactt caggaaaagc tcctcccaac ctccctcctg
     6781 gggtcccgcc gttgttgcct aatccgtata ttatggctcc agggctgtta catgcctacc
     6841 cgccacaagt atatggttat gatgacttgc agatgcttca gacaagattt ccattggatt
     6901 actacagcat cccatttccc acacccacta ctccgctgac tgggagggat ggtagcctgg
     6961 ccagcaaccc ttattctggt gacctcacaa agttcggccg tggggatgcc tcctccccag
     7021 ccccggccac aaccttggcc caaccccaac agaaccagac gcagactcac cataccacgc
     7081 agcagacatt cctgaacccg gcgctgcctc ctggctacag ttacaccagc ctgccatact
     7141 atacaggggt cccgggcctc cccagcacct tccagtatgg gcctgctgtg ttccctgtgg
     7201 ctcctacctc ttccaagcag catggtgtga atgtcagtgt gaatgcatcg gccacccctt
     7261 tccaacagcc gagtggatat gggtctcatg gatacaacac tggtgtttca gtcacctcca
     7321 gtaacacggg cgtgccagat atctcgggtt ctgtgtactc caaaacccag cagtcctttg
     7381 agaaacaagg ttttcattcc ggtactcctg ctgcttcctt caacttgcct tcagccctag
     7441 gaagtggggg ccccatcaat ccggccacag ctgctgccta cccacctgcc ccctttatgc
     7501 acattctgac cccccatcag cagccgcatt ctcagatcct tcaccatcac ctgcagcagg
     7561 atggccagac gggcagcggg caacgtagcc agaccagctc catcccgcag aagccccaga
     7621 ccaacaagtc tgcctacaac agctacagct ggggggccaa cgaattccac caccaccacc
     7681 accactaact gcaggtaatt aaataagctt caaataaaac gaaaggctca gtcgaaagac
     7741 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     7801 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg
     7861 ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg
     7921 tttctacaaa ctctttcggt ccgttgttta tttttctaaa tacattcaaa tatgtatccg
     7981 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     8041 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     8101 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     8161 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8221 cgtttcccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     8281 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8341 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8401 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8461 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8521 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8581 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8641 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8701 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8761 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8821 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8881 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttcctt
     8941 aggactgagc gtcaaccccg tagaaaagat caaaggatct tc
//