| 质粒名称: | CMV-T7-hOsa2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 17987 |
| 存储实验室: | Naoko Tanese |
|
载体骨架基本信息 |
|
| 载体名称: | pCGT |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 未知 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Osa2,BAF250b |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 5500 bp |
| 基因突变: | -- |
| GenBank编号 | AF521671 |
| 启动子 | -- |
| 标签/融合蛋白: | T7表位（主干N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Xba I（未销毁） |
| 3' 克隆位点: | BgI I I/BamH I（在克隆过程中被销毁） |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | CMV-T7-hOsa2 was a gift from Naoko Tanese (Addgene plasmid # 17987 ; http://n2t.net/addgene:17987 ; RRID:Addgene_17987) |
| 参考文献部分: | Largest subunits of the human SWI/SNF chromatin-remodeling complex promote transcriptional activation by steroid hormone receptors. Inoue H, Furukawa T, Giannakopoulos S, Zhou S, King DS, Tanese N. J Biol Chem. 2002 Nov 1. 277(44):41674-85. 10.1074/jbc.M205961200 PubMed 12200431 |
买家导航
CMV-T7-hOsa2载体质粒基本信息
CMV-T7-hOsa2质粒图谱载体图谱和CMV-T7-hOsa2载体序列质粒序列多克隆位点信息
CMV-T7-hOsa2质粒载体简介
CMV-T7-hOsa2质粒序列载体序列
LOCUS CMV-T7-hOsa2 10561 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS CMV-T7-hOsa2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10561)
AUTHORS Inoue H, Furukawa T, Giannakopoulos S, Zhou S, King DS, Tanese N
TITLE Largest subunits of the human SWI/SNF chromatin-remodeling complex
promote transcriptional activation by steroid hormone receptors.
JOURNAL J Biol Chem. 2002 Nov 1. 277(44):41674-85.
PUBMED 12200431
REFERENCE 2 (bases 1 to 10561)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10561
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(30..52)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 152..171
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
rep_origin complement(238..693)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(375..396)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 587..606
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 819..841
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 833..850
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 834..850
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 857..876
/label=T7
/note="T7 promoter, forward primer"
promoter 857..875
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
enhancer 894..1197
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1198..1401
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 1352..1372
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 1398..1422
/label=LNCX
/note="Human CMV promoter, forward primer"
5'UTR 1491..1530
/label=HSV TK 5'-UTR
/note="5' untranslated region from the herpes simplex virus
thymidine kinase gene"
intron 7153..7725
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
primer_bind complement(7857..7876)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 7922..7977
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(7976..7995)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
rep_origin 8331..8466
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(8385..8404)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(8572..8589)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8743..9331)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8823..8842)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9502..10362)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10125..10144
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10363..10467)
/gene="bla"
/label=AmpR promoter
primer_bind 10535..10553
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
61 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
121 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
181 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa
241 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
301 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
361 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
421 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
481 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
541 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
601 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
661 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg
721 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
781 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
841 gacggccagt gaattgtaat acgactcact atagggcgaa ttcgagctcg ccccgttaca
901 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
961 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
1021 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
1081 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
1141 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
1201 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
1261 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
1321 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
1381 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
1441 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgggg gatcttggtg
1501 gcgtgaaact cccgcacctc ttcggccagc gccttgtaga agcgcgtatg gcttctagga
1561 tggcatcgat gacaggtggc caacagatgg gttctagagc ctgggcggcc gcgcaacaaa
1621 ggagtcaccc ggcgatgagc cccggcaccc ccggaccgac catgggcaga tcccagggca
1681 gcccaatgga tccaatggtg atgaagagac ctcagttgta tggcatgggc agtaaccctc
1741 attctcagcc tcagcagagc agtccgtacc caggaggttc ctatggccct ccaggcccac
1801 agcggtatcc aattggcatc cagggtcgga ctcccggggc catggccgga atgcagtacc
1861 ctcagcagca gatgccacct cagtatggac agcaaggtgt gagtggttac tgccagcagg
1921 gccaacagcc atattacagc cagcagccgc agcccccgca cctcccaccc caggcgcagt
1981 atctgccgtc ccagtcccag cagaggtacc agccgcagca ggacatgtct caggaaggct
2041 atggaactag atctcaacct cctctggccc ccggaaaacc taaccatgaa gacttgaact
2101 taatacagca agaaagacca tcaagtttac cagatctgtc tggctccatt gatgacctcc
2161 ccacgggaac ggaagcaact ttgagctcag cagtcagtgc atccgggtcc acgagcagcc
2221 aaggggatca gagcaacccg gcgcagtcgc ctttctcccc acatgcgtcc cctcatctct
2281 ccagcatccc ggggggccca tctccctctc ctgttggctc tcctgtagga agcaaccagt
2341 ctcgatctgg cccaatctct cctgcaagta tcccaggtag tcagatgcct ccgcagccac
2401 ccgggagcca gtcagaatcc agttcccatc ccgccttgag ccagtcacca atgccacagg
2461 aaagaggttt tatggcaggc acacaaagaa accctcagat ggctcagtat ggacctcaac
2521 agacaggacc atccatgtcg cctcatcctt ctcctggggg ccagatgcat gctggaatca
2581 gtagctttca gcagagtaac tcaagtggga cttacggtcc acagatgagc cagtatggac
2641 cacaaggtaa ctactccaga cccccagcgt atagtggggt gcccagtgca agctacagcg
2701 gcccagggcc cggtatgggt atcagtgcca acaaccagat gcatggacaa gggccaagcc
2761 agccatgtgg tgctgtgccc ctgggacgaa tgccatcagc tgggatgcag aacagaccat
2821 ttcctggaaa tatgagcagc atgaccccca gttctcctgg catgtctcag cagggagggc
2881 caggaatggg gccgccaatg ccaactgtga accgtaaggc acaggaggca gccgcagcag
2941 tgatgcaggc tgctgcgaac tcagcacaaa gcaggcaagg cagtttcccc ggcatgaacc
3001 agagtggact tatggcttcc agctctccct acagccagcc catgaacaac agctctagcc
3061 tgatgaacac gcaggcgccg ccctacagca tggcgcccgc catggtgaac agctcggcag
3121 catctgtggg tcttgcagat atgatgtctc ctggtgaatc caaactgccc ctgcctctca
3181 aagcagacgg caaagaagaa ggcactccac agcccgagag caagtcaaag aagtccagct
3241 cctccaccac tactggggag aagatcacga aggtgtacga gctggggaat gagccagaga
3301 gaaagctctg ggtcgaccga tacctcacct tcatggaaga gagaggctct cctgtctcaa
3361 gtctgcctgc cgtgggcaag aagcccctgg acctgttccg actctacgtc tgcgtcaaag
3421 agatcggggg tttggcccag gttaataaaa acaagaagtg gcgtgagctg gcaaccaacc
3481 taaacgttgg cacctcaagc agtgcagcga gctccctgaa aaagcagtat attcagtacc
3541 tgtttgcctt tgagtgcaag atcgaacgtg gggaggagcc cccgccggaa gtcttcagca
3601 ccggggacac caaaaagcag cccaagctcc agccgccatc tcctgctaac tcgggatcct
3661 tgcaaggccc acagaccccc cagtcaactg gcagcaattc catggcagag gttccaggtg
3721 acctgaagcc acctacccca gcctccaccc ctcacggcca gatgactcca atgcaaggtg
3781 gaagaagcag tacaatcagt gtgcacgacc cattctcaga tgtgagtgat tcatccttcc
3841 cgaaacggaa ctccatgact ccaaacgccc cctaccagca gggcatgagc atgcccgatg
3901 tgatgggcag gatgccctat gagcccaaca aggacccctt tgggggaatg agaaaagtgc
3961 ctggaagcag cgagcccttt atgacgcaag gacagatgcc caacagcagc atgcaggaca
4021 tgtacaacca aagtccctcc ggagcaatgt ctaacctggg catggggcag cgccagcagt
4081 ttccctatgg agccagttac gaccgaaggc atgaacctta tgggcagcag tatccaggcc
4141 aaggccctcc ctcgggacag ccgccgtatg gagggcacca gcccggcctg tacccacagc
4201 agccgaatta caaacgccat atggacggca tgtacgggcc cccagccaag cgccacgagg
4261 gcgacatgta caacatgcag tacagcagcc agcagcagga gatgtacaac cagtatggag
4321 gctcctactc gggcccggac cgcaggccca tccagggcca gtacccgtat ccctacagca
4381 gggagaggat gcagggcccg gggcagatcc agacacacgg aatcccgcct cagatgatgg
4441 gcggcccgct gcagtcgtcc tccagtgagg ggcctcagca gaatatgtgg gcagcacgca
4501 atgatatgcc ttatccctac cagaacaggc agggccctgg cggccctaca caggcgcccc
4561 cttacccagg catgaaccgc acagacgata tgatggtacc cgatcagagg ataaatcatg
4621 agagccagtg gccttctcac gtcagccagc gtcagcctta tatgtcgtcc tcagcctcca
4681 tgcagcccat cacacgccca ccacagccgt cctaccagac gccaccgtca ctgccaaatc
4741 acatctccag ggcgcccagc ccagcgtcct tccagcgctc cctggagaac cgcatgtctc
4801 caagcaagtc tccttttctg ccgtctatga agatgcagaa ggtcatgccc acggtcccca
4861 catcccaggt caccgggcca ccaccccaac cacccccaat cagaagggag atcacctttc
4921 ctcctggctc agtagaagca tcacaaccag tcttgaaaca aaggcgaaag attacctcca
4981 aagatatcgt tactcctgag gcgtggcgtg tgatgatgtc ccttaaatca ggtcttttgg
5041 ctgagagtac gtgggctttg gacactatta atattcttct gtatgatgac agcactgttg
5101 ctactttcaa tctctcccag ttgtctggat ttctcgaact tttagtcgag tactttagaa
5161 aatgcctgat tgacattttt ggaattctta tggaatatga agtgggagac cccagccaaa
5221 aagcacttga tcacaacgca gcaaggaagg atgacagcca gtccttggca gacgattctg
5281 ggaaagagga ggaagatgct gaatgtattg atgacgacga ggaagacgag gaggatgagg
5341 aggaagacag cgagaagaca gaaagcgatg aaaagagcag catcgctctg actgccccgg
5401 acgccgctgc agacccaaag gagaagccca agcaagccag taagttcgac aagctgccaa
5461 taaagatagt caaaaagaac aacctgtttg ttgttgaccg atctgacaag ttggggcgtg
5521 tgcaggagtt caatagtggc cttctgcact ggcagctcgg cgggggtgac accaccgagc
5581 acattcagac tcactttgag agcaagatgg aaattcctcc tcgcaggcgc ccacctcccc
5641 ccttaagctc cgcaggtaga aagaaagagc aagaaggcaa aggcgactct gaagagcagc
5701 aagagaaaag catcatagca accatcgatg acgtcctctc tgctcggcca ggggcattgc
5761 ctgaagacgc aaaccctggg ccccagaccg aaagcagtaa gtttcccttt ggtatccagc
5821 aagccaaaag tcaccggaac atcaagctgc tggaggacga gcccaggagc cgagacgaga
5881 ctcctctgtg taccatcgcg cactggcagg actcgctggc taagcgatgc atctgtgtgt
5941 ccaatattgt ccgtagcttg tcattcgtgc ctggcaatga tgccgaaatg tccaaacatc
6001 caggcctggt gctgatcctg gggaagctga ttcttcttca ccacgagcat ccagagagaa
6061 agcgagcacc gcagacctat gagaaagagg aggatgagga caagggggtg gcctgcagca
6121 aagatgagtg gtggtgggac tgcctcgagg tcttgaggga taacacgttg gtcacgttgg
6181 ccaacatttc cgggcagcta gacttgtctg cttacacgga aagcatctgc ttgccaattt
6241 tggatggctt gctgcactgg atggtgtgcc cgtctgcaga ggcacaagat ccctttccaa
6301 ctgtgggacc caactcggtc ctgtcgcctc agagacttgt gctggagacc ctctgtaaac
6361 tcagtatcca ggacaataat gtggacctga tcttggccac tcctccattt agtcgtcagg
6421 agaaattcta tgctacatta gttaggtacg ttggggatcg caaaaaccca gtctgtcgag
6481 aaatgtccat ggcgctttta tcgaaccttg cccaagggga cgcactagca gcaagggcca
6541 tagctgtgca gaaaggaagc attggaaact tgataagctt cctagaggat ggggtcacga
6601 tggcccagta ccagcagagc cagcacaacc tcatgcacat gcagcccccg cccctggaac
6661 cacctagcgt agacatgatg tgcagggcgg ccaaggcttt gctagccatg gccagagtgg
6721 acgaaaaccg ctcggaattc cttttgcacg agggccggtt gctggatatc tcgatatcag
6781 ctgtcctgaa ctctctggtt gcatctgtca tctgtgatgt actgtttcag attgggcagt
6841 tatgacataa gtgagaaggc aagcatgtgt gagtgaagat tagagggtca catataactg
6901 gctgttttct gttcttgttt atccagcgta ggaagaagga aaagaaaatc tttgctcctc
6961 tgccccattc actatttacc aattgggaat taaagaaata attaatttga acagttatga
7021 aattaatatt tgctgtctgt gtgtataagt acatcctttg gggttttttt tttctctttt
7081 ttttaaccaa agttgctgtc tagtgcattc aaaggtcact ttttgttctt cacagatcct
7141 gagaacttca gggtgagttt ggggaccctt gattgttctt tctttttcgc tattgtaaaa
7201 ttcatgttat atggaggggg caaagttttc agggtgttgt ttagaatggg aagatgtccc
7261 ttgtatcacc atggaccctc atgataattt tgtttctttc actttctact ctgttgacaa
7321 ccattgtctc ctcttatttt cttttcattt tctgtaactt tttcgttaaa ctttagcttg
7381 catttgtaac gaatttttaa attcactttt gtttatttgt cagattgtaa gtactttctc
7441 taatcacttt tttttcaagg caatcagggt atattatatt gtacttcagc acagttttag
7501 agaacaattg ttataattaa atgataaggt agaatatttc tgcatataaa ttctggctgg
7561 cgtggaaata ttcttattgg tagaaacaac tacaccctgg tcatcatcct gcctttctct
7621 ttatggttac aatgatatac actgtttgag atgaggataa aatactctga gtccaaaccg
7681 ggcccctctg ctaaccatgt tcatgccttc ttctctttcc tacagctcct gggcaacgtg
7741 ctggttgttg tgctgtctca tcattttggc aaagaattca ctcctcaggt gcaggctgcc
7801 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
7861 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
7921 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
7981 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
8041 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
8101 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
8161 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
8221 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
8281 gtccctcttc tcttatggag atccctcgag gagctttttg caaaagccta ggcctccaaa
8341 aaagcctctt cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata
8401 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
8461 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgctgc attaatgaat
8521 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
8581 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
8641 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
8701 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
8761 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
8821 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
8881 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
8941 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
9001 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
9061 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
9121 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
9181 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
9241 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
9301 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
9361 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
9421 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
9481 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
9541 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
9601 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
9661 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
9721 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
9781 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
9841 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
9901 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
9961 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
10021 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
10081 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
10141 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
10201 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
10261 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
10321 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
10381 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
10441 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
10501 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
10561 t
//
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