| 质粒名称: | pAAV-TNT4-Zfr-P2A-HA-YAP5SA |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001193 |
| 产品用途: | in vivo |
| 存储实验室: | Yuxuan Guo Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒表达 |
| 启动子: | hTNNT2 |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
|
插入基因信息 |
|
| 插入基因名称: | YAP5SA |
| 物种来源: | MUS |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Neural Organoids Protect Engineered Heart Tissues From Glucolipotoxicity by Transferring Versican in a Co-Culture System.
Bai B., Li J., Wang Z., Yang Y., He J., Chen G., Zhang Y., Qi Y., Wan Z., Cai L., Wang R., Wang K., Zhao D., Zhang J., Huang W., Xu X.R., Sun M., Han X., Liu Y., Zhang D., Zhu W., Liu J., Guo Y.
Cell Prolif. 0, e70070 (2025)
|
买家导航
pAAV-TNT4-Zfr-P2A-HA-YAP5SA载体质粒基本信息
pAAV-TNT4-Zfr-P2A-HA-YAP5SA质粒图谱载体图谱和pAAV-TNT4-Zfr-P2A-HA-YAP5SA载体序列质粒序列多克隆位点信息
pAAV-TNT4-Zfr-P2A-HA-YAP5SA质粒载体简介
pAAV-TNT4-Zfr-P2A-HA-YAP5SA质粒序列载体序列
LOCUS pAAV-TNT4-Zfr-P2A-HA-YAP5SA 6768 bp ds-DNA circular 21-JUN-2024
DEFINITION .
COMMENT ^^
FEATURES Location/Qualifiers
misc_feature 1..130
/label="5' ITR"
/ApEinfo_revcolor="#d59687"
/ApEinfo_fwdcolor="#d59687"
misc_feature 29..34
/label="XmaI"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
misc_feature 40..45
/label="XmaI"
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
misc_feature 176..197
/label="overlap"
/ApEinfo_revcolor="#ffef86"
/ApEinfo_fwdcolor="#ffef86"
misc_feature 178..197
/label="nhe1cut"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 198..218
/label="overlap"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 198..741
/label="hTNT promoter"
/ApEinfo_revcolor="#faac61"
/ApEinfo_fwdcolor="#faac61"
misc_feature 733..756
/label="NOT1 cut"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
primer 737..777
/label="tnt4-zn-clone-F"
/note="sequence: GGCAGagctCCTCGAGAAGcACTGACCAACAGCGTGAAGAC"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
misc_feature 757..777
/label="overlap"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 757..795
/label="upstream"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
exon 757..795
/label="Zfr-201 Exon 19"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
gene 757..1989
/label="Zfr minigene(1233BP)"
/ApEinfo_revcolor="#b1ff67"
/ApEinfo_fwdcolor="#b1ff67"
primer 769..792
/label="Zfr-rtpcr-F"
/note="sequence: CGTGAAGACATTACATCCAGTGCA"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 841..930
/label="I1D1"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
misc_feature 841..1200
/label="to delete"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
misc_feature 931..1020
/label="I1D2"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 1021..1110
/label="I1D3"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
misc_feature 1111..1200
/label="I1D4"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
misc_feature 1261..1264
/label="bp"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 1296..1379
/label="PSEX"
/ApEinfo_revcolor="#faac61"
/ApEinfo_fwdcolor="#faac61"
CDS 1299..1379
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="KGLHIKNIV*TL*GQRSVLISPD*KL*"
primer 1345..1369
/label="Zfr-qpcr-F"
/note="sequence: GAAGTGTGCTCATTTCTCCCGATTA"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
CDS 1376..1379
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="M"
misc_feature 1380..1948
/label="keep"
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
misc_feature 1433..1522
/label="I2D1"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 1433..1882
/label="to delete"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
misc_feature 1523..1612
/label="I2D2"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
misc_feature 1613..1702
/label="I2D3"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 1703..1792
/label="I2D4"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 1793..1882
/label="I2D5"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
misc_feature 1933..1937
/label="bp"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
exon 1949..1989
/label="Zfr-201 Exon 20"
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
misc_feature 1949..1989
/label="downstream"
/ApEinfo_revcolor="#b4abac"
/ApEinfo_fwdcolor="#b4abac"
CDS 1951..1989
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="CIETPCIPSDTQS"
misc_feature 1966..1989
/label="overlap"
/ApEinfo_revcolor="#d59687"
/ApEinfo_fwdcolor="#d59687"
misc_feature 1990..2046
/label="P2A"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
CDS 1990..2046
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="ATNFSLLKQAGDVEENPGP"
primer complement(2003..2025)
/label="P2A-R1"
/note="sequence: gtctccagcctgTttTagTaggc"
/ApEinfo_revcolor="#b4abac"
/ApEinfo_fwdcolor="#b4abac"
primer complement(2005..2029)
/label="P2A-R2"
/note="sequence: cAacgtctccagcctgTttTagTag"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 2007..2028
/label="non-AUG start codons removed"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
primer complement(2011..2031)
/label="P2A-R5"
/note="sequence: ctcAacgtctccagcctgTtt"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
primer complement(2017..2037)
/label="P2A-R3"
/note="sequence: gttctcctcAacgtctccagc"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
primer complement(2025..2044)
/label="P2A-R4"
/note="sequence: gtccagggttctcctcAacg"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
CDS 2047..2070
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="YPYDVPDY"
misc_feature 2047..2073
/label="HA tag"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
misc_feature 2071..2089
/label="NOT1 cut"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
CDS 2071..3543
/label="Translation 2071-3543"
/translation="ADPGQQPPPQPAPQGQGQPPSQPPQGQGPPSGPGQPAPAATQAAPQAPPAGHQIVHVRGDAETDLEALFNAVMNPKTANVPQTVPMRLRKLPDSFFKPPEPKSHSRQAATDAGTAGALTPQHVRAHAAPAALQLGAVSPGTLTPTGVVSGPAATPTAQHLRQAAFEIPDDVPLPAGWEMAKTSSGQRYFLNHIDQTTTWQDPRKAMLSQMNVTAPTSPPVQQNMMNSASGPLPDGWEQAMTQDGEIYYINHKNKTTSWLDPRLDPRFAMNQRISQSAPVKQPPPLAPQSPQGGVMGGSNSNQQQQMRLQQLQMEKERLRLKQQELLRQELALRSQLPTLEQDGGTQNPVSSPGMSQELRTMTTNSSDPFLNSGTYHSRDEATDSGLSMSSYSVPRTPDDFLNSVDEMDTGDTINQSTLPSQQNRFPDYLEAIPGTNVDLGTLEGDGMNIEGEELMPSLQEALSSDILNDMESVLAATKLDKESFLTWLYL*"
exon 2073..2391
/label="YAP1"
/ApEinfo_revcolor="#faac61"
/ApEinfo_fwdcolor="#faac61"
CDS 2073..3533
/label="YAP1"
/ApEinfo_revcolor="#b1ff67"
/ApEinfo_fwdcolor="#b1ff67"
misc_feature 2251..2253
/label="YAP1"
/ApEinfo_revcolor="#b4abac"
/ApEinfo_fwdcolor="#b4abac"
misc_feature 2257..2259
/label="YAP1"
/ApEinfo_revcolor="#c7b0e3"
/ApEinfo_fwdcolor="#c7b0e3"
PCR_primer 2266..2292
/label="hYAP-seq-F1"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 2383..2385
/label="YAP1"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
exon 2392..2642
/label="YAP1"
/ApEinfo_revcolor="#faac61"
/ApEinfo_fwdcolor="#faac61"
misc_feature 2395..2397
/label="YAP1"
/ApEinfo_revcolor="#b1ff67"
/ApEinfo_fwdcolor="#b1ff67"
misc_feature 2398..2400
/label="YAP1"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
misc_feature 2425..2427
/label="YAP1"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
misc_feature 2449..2451
/label="Zhiqiang's S127A mutation"
/ApEinfo_revcolor="#b7e6d7"
/ApEinfo_fwdcolor="#b7e6d7"
misc_feature 2449..2451
/label="YAP1"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 2452..2454
/label="YAP1"
/ApEinfo_revcolor="#b7e6d7"
/ApEinfo_fwdcolor="#b7e6d7"
misc_feature 2461..2463
/label="YAP1"
/ApEinfo_revcolor="#c7b0e3"
/ApEinfo_fwdcolor="#c7b0e3"
misc_feature 2482..2484
/label="YAP1"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 2530..2532
/label="YAP1"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 2557..2559
/ApEinfo_revcolor="#c7b0e3"
/ApEinfo_fwdcolor="#c7b0e3"
misc_feature 2560..2562
/label="YAP1"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
exon 2643..2758
/label="YAP1"
/ApEinfo_revcolor="#faac61"
/ApEinfo_fwdcolor="#faac61"
exon 2759..2872
/label="YAP1"
/ApEinfo_revcolor="#ffef86"
/ApEinfo_fwdcolor="#ffef86"
PCR_primer 2844..2873
/label="hYAP-Seq-F2"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
exon 2873..3054
/label="YAP1"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 2890..2892
/label="YAP1"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
misc_feature 2935..2937
/label="YAP1"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
misc_feature 2941..3533
/label="YAP1"
/ApEinfo_revcolor="#faac61"
/ApEinfo_fwdcolor="#faac61"
misc_feature 3053..3058
/label="skip one exon here in Zhiqiang's plasmid"
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
exon 3055..3055
/label="YAP1"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
exon 3056..3185
/label="YAP1"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
misc_feature 3121..3123
/label="YAP1"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 3133..3135
/label="YAP1"
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
misc_feature 3166..3168
/label="YAP1"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
exon 3186..3298
/label="YAP1"
/ApEinfo_revcolor="#b1ff67"
/ApEinfo_fwdcolor="#b1ff67"
misc_feature 3211..3213
/label="YAP1"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 3220..3222
/label="YAP1"
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
misc_feature 3229..3231
/label="YAP1"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 3241..3243
/label="YAP1"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 3256..3258
/label="YAP1"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
exon 3299..3533
/label="YAP1"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
PCR_primer 3370..3394
/label="hYAP-Seq-F3"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 3505..3533
/label="overlap"
/ApEinfo_revcolor="#ffef86"
/ApEinfo_fwdcolor="#ffef86"
misc_feature 3544..3562
/label="cutting site"
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
misc_feature 3560..3565
/label="XmaI"
/ApEinfo_revcolor="#b1ff67"
/ApEinfo_fwdcolor="#b1ff67"
terminator 3603..3729
/label="PolyA"
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
misc_feature 3818..3947
/label="3' ITR"
/ApEinfo_revcolor="#b7e6d7"
/ApEinfo_fwdcolor="#b7e6d7"
misc_feature 3903..3908
/label="XmaI"
/ApEinfo_revcolor="#c7b0e3"
/ApEinfo_fwdcolor="#c7b0e3"
misc_feature 3914..3919
/label="XmaI"
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
rep_origin 4124..4579
/label="f1 origin"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 4605..4709
/label="Amp Promoter"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 4710..5567
/label="Amp resistent"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
rep_origin 5741..6329
/label="origin"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
ORIGIN
1 CTGCGCGCTC GCTCGCTCAC TGAGGCCGCC CGGGCAAAGC CCGGGCGTCG GGCGACCTTT
61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
121 AGGGGTTCCT TGTAGTTAAT GATTAACCCG CCATGCTACT TATCTACCAG GGTAATGGGG
181 ATCCTCTAGA ACTATAGCTC AGTCCATTAG GAGCCAGTAG CCTGGAAGAT GTCTTTACCC
241 CCAGCATCAG TTCAAGTGGA GCAGCACATA ACTCTTGCCC TCTGCCTTCC AAGATTCTGG
301 TGCTGAGACT TATGGAGTGT CTTGGAGGTT GCCTTCTGCC CCCCAACCCT GCTCCCAGCT
361 GGCCCTCCCA GGCCTGGGTT GCTGGCCTCT GCTTTATCAG GATTCTCAAG AGGGACAGCT
421 GGTTTATGTT GCATGACTGT TCCCTGCATA TCTGCTCTGG TTTTAAATAG CTTATCTGAG
481 CAGCTGGAGG ACCACATGGG CTTATATGGC GTGGGGTACA TGTTCCTGTA GCCTTGTCCC
541 TGGCACCTGC CAAAATAGCA GCCAACAccc cccaccccca ccgccatccc cctgccccac
601 ccgtccccTG TCGCACATTC CTCCCTCCGC AGGGCTGGCT CACCAGGCCC CAGCCCACAT
661 GCCTGCTTAA AGCCCTCTCC ATCCTCTGCC TCACCCAGTC CCCGCTGAGA CTGAGCAGAC
721 GCCTCCAGGA TCTGTCGGCA GagctCCTCG AGAAGcACTG ACCAACAGCG TGAAGACATT
781 ACATCCAGTG CACAGGTAAT TGAATTCTTG TTGGATTACT GTCTCTAAGG CCTGGGAGTT
841 TTATAATTGT TTCTTTATGT GTAATAATAA CCGTACTAGT CATTGTGATA CTTATTTGGG
901 AAGTAAGCCT TTAAAGAGGT TATTAAAAAG ATTTACTGAT TCCCACACCG GAAGTTGCTC
961 TTCTAGCCGT GAAAGAAGGA CGGCGTGGTC TTCAGTTTGC TATGTCCACT AACAATATGT
1021 CCGACCCACG AAGGCCCACC AAAGTTATAC TTGTGGCACA TATAAACCTT TGGAATCAAG
1081 GGACCTGATA CTGGATTATT AAATATTTAA GAGAATTTAT ATTTAATAAA CTTGATACTT
1141 CAGTGAGAAA GGACAAATCA TCAGTAGAAT GGTTTTGAGA TAGCTGATGA TTCTATTAAA
1201 AGTAGATATG TGTGTGGGTT AAATGACTTA AGCATACATC AAAAGACTGA GAAAGGCTCT
1261 TAACATTAAA AGATGGGCCt ttttttttCT TTTAGGTTAA AGGATTACAT ATTAAAAATA
1321 TTGTATAGAC TCTTTAAGGC CAAAGAAGTG TGCTCATTTC TCCCGATTAA AAGTTATGAG
1381 TAAGAATTGG GTATTTTGCC TTTTGGGGGT GGGAGAtttg aaacagggtt tctccttaca
1441 gctctggctt tcctggagca gtgaattgag cctggctcct ctgtaggagt aacgagttgc
1501 tttaaccact gacatctttc caccaccacc accaccCCTT ATGAATAGAC TAAATTTATT
1561 TAAAGATTGA TATTTTCTAG CTTAATTATT TGGAAAGCAA ATAAGCACAC AGAAAAGCCT
1621 CCTCCCCaaa aaaatataaa taaaaaaGTC TATCAGGACA TAGTATATGT CCCTTCTGAT
1681 TAATCATTGG TGGTGAAACA ACCCATTCAG GGTGTCTCTT CCATCACAGT GGAttttttt
1741 CATATTGTTA CATGGAGAAG TTAACTTAGT AAGAATTGAT GTGCTTCTGT GCCAGTGAGA
1801 TAGCTCTGCA GGTAAAAGGG AGACTGACCC CCAACTTGTA CTCTGGCCGT GGCAGTCATG
1861 TGCACACAAG TAAACTGAAT AAATATAAAG AGCAGATTTT AGAGTGTTTT GGCTTTAAAT
1921 TAATTCTATA TTCTAACTCT ATTTGCAGTT TGCATTGAGA CTCCTTGCAT TCCGTCAGAT
1981 ACACAAAGTg ctactaactt cagcctActA aaAcaggctg gagacgtTga ggagaaccct
2041 ggacctTATC CATAcGACGT TCCAGATTAC GCagatcccg ggcagcagcc gccgcctcaa
2101 ccggcccccc agggccaagg gcagccgcct tcgcagcccc cgcaggggca gggcccgccg
2161 tccggacccg ggcaaccggc acccgcggcg acccaggcgg cgccgcaggc accccccgcc
2221 gggcatcaga tcgtgcacgt ccgcggggac gcggagaccg acctggaggc gctcttcaac
2281 gccgtcatga accccaagac ggccaacgtg ccccagaccg tgcccatgag gctccggaag
2341 ctgcccgact ccttcttcaa gccgccggag cccaaatccc actcccgaca ggccgctact
2401 gatgcaggca ctgcaggagc cctgactcca cagcatgttc gagctcatgc cgctccagct
2461 gctctgcagt tgggagctgt ttctcctggg acactgaccc ccactggagt agtctctggc
2521 ccagcagcta cacccacagc tcagcatctt cgacaggctg cttttgagat acctgatgat
2581 gtacctctgc cagcaggttg ggagatggca aagacatctt ctggtcagag atacttctta
2641 aatcacatcg atcagacaac aacatggcag gaccccagga aggccatgct gtcccagatg
2701 aacgtcacag cccccaccag tccaccagtg cagcagaata tgatgaactc ggcttcaggt
2761 cctcttcctg atggatggga acaagccatg actcaggatg gagaaattta ctatataaac
2821 cataagaaca agaccacctc ttggctagac ccaaggcttg accctcgttt tgccatgaac
2881 cagagaatca gtcagagtgc tccagtgaaa cagccaccac ccctggctcc ccagagccca
2941 cagggaggcg tcatgggtgg cagcaactcc aaccagcagc aacagatgcg actgcagcaa
3001 ctgcagatgg agaaggagag gctgcggctg aaacagcaag aactgcttcg gcaggAgtta
3061 gccctgcgta gccagttacc aacactggag caggatggtg ggactcaaaa tccagtgtct
3121 tctcccggga tgtctcagga attgagaaca atgacgacca atagctcaga tcctttcctt
3181 aacagtggca cctatcactc tcgagatgag gctacagaca gtggactaag catgagcagc
3241 tacagtgtcc ctcgaacccc agatgacttc ctgaacagtg tggatgagat ggatacaggt
3301 gatactatca accaaagcac cctgccctca cagcagaacc gtttcccaga ctaccttgaa
3361 gccattcctg ggacaaatgt ggaccttgga acactggaag gagatggaat gaacatagaa
3421 ggagaggagc tgatgccaag tctgcaggaa gctttgagtt ctgacatcct taatgacatg
3481 gagtctgttt tggctgccac caagctagat aaagaaagct ttcttacatg gttGTACCTC
3541 TAGGTACCTC TAGAGTCGAC CCGGGCGGCC TCGAGGACGG GGTGAACTAC GCCTGAGGAT
3601 CCGATCTTTT TCCCTCTGCC AAAAATTATG GGGACATCAT GAAGCCCCTT GAGCATCTGA
3661 CTTCTGGCTA ATAAAGGAAA TTTATTTTCA TTGCAATAGT GTGTTGGAAT TTTTTGTGTC
3721 TCTCACTCGG AAGCAATTCG TTGATCTGAA TTTCGACCAC CCATAATACC CATTACCCTG
3781 GTAGATAAGT AGCATGGCGG GTTAATCATT AACTACAAGG AACCCCTAGT GATGGAGTTG
3841 GCCACTCCCT CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG GGCGACCAAA GGTCGCCCGA
3901 CGCCCGGGCT TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG CGCGCAGCCT TAATTAACCT
3961 AATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT
4021 AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC
4081 GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGGACGCGCC CTGTAGCGGC
4141 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC
4201 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC
4261 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC
4321 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG
4381 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT
4441 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT
4501 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA
4561 ATATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT
4621 GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA
4681 TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA
4741 TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG
4801 TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA
4861 GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA
4921 AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC
4981 GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC
5041 TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA
5101 CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC
5161 ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA
5221 TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC
5281 TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG
5341 CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG
5401 ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG
5461 GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC
5521 GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC
5581 AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT
5641 AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC
5701 ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC
5761 GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG
5821 ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA
5881 ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC
5941 CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT
6001 GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA
6061 CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC
6121 TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC
6181 CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT
6241 GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT
6301 GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC
6361 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG
6421 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC
6481 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG
6541 CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA
6601 GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT
6661 TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA
6721 ACAGCTATGA CCATGATTAC GCCAGATTTA ATTAAGGCCT TAATTAGG
//
pAAV-TNT4-Zfr-P2A-HA-YAP5SA载体图谱质粒图谱pdf版和pAAV-TNT4-Zfr-P2A-HA-YAP5SA载体序列质粒序列等相关资料下载
pAAV-TNT4-Zfr-P2A-HA-YAP5SA质粒载体应用举例
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