pQL61

LOCUS      pQL61                6434 bp DNA     circular SYN 12-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6434
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     primer_bind     141..160
                     /label=测序-F
     primer_bind     213..261
                     /label=QL119
     misc_feature    234..261
                     /label=GGAA
     primer_bind     complement(242..282)
                     
     misc_feature    268..415
                     /label=hcmvmin
     misc_feature    337..351
                     /label=New Feature
     primer_bind     complement(416..437)
                     /label=测序-R
     misc_feature    438..461
                     /label=New Feature
     misc_feature    463..1983
                     /label=SEAP
     terminator      2031..2258
                     /label=bGH PA
     misc_feature    2926..3003
                     /label=SV40 ORI
     CDS             3145..3936
                     /label=Kan/neoR
     primer_bind     complement(4276..4296)
                     /label=M13-rev
     misc_binding    complement(4302..4324)
                     /label=LacO
     promoter        complement(4329..4358)
                     /label=lac
     CDS             complement(5441..6100)
                     /label=AmpR
     promoter        complement(6340..6368)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgtacgga
      241 aggaaggaag gaaggaagat cgctagctcg agctcggtac ccgggtcgag taggcgtgta
      301 cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      361 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggcccc
      421 gaattcgagc tcgcccgggg atcctctaga gtcagcttct gcatgctgct gctgctgctg
      481 ctgctgggcc tgaggctaca gctctccctg ggcatcatcc cagttgagga ggagaacccg
      541 gacttctgga accgcgaggc agccgaggcc ctgggtgccg ccaagaagct gcagcctgca
      601 cagacagccg ccaagaacct catcatcttc ctgggcgatg ggatgggggt gtctacggtg
      661 acagctgcca ggatcctaaa agggcagaag aaggacaaac tggggcctga gatacccctg
      721 gccatggacc gcttcccata tgtggctctg tccaagacat acaatgtaga caaacatgtg
      781 ccagacagtg gagccacagc cacggcctac ctgtgcgggg tcaagggcaa cttccagacc
      841 attggcttga gtgcagccgc ccgctttaac cagtgcaaca cgacacgcgg caacgaggtc
      901 atctccgtga tgaatcgggc caagaaagca gggaagtcag tgggagtggt aaccaccaca
      961 cgagtgcagc acgcctcgcc agccggcacc tacgcccaca cggtgaaccg caactggtac
     1021 tcggacgccg acgtgcctgc ctcggcccgc caggaggggt gccaggacat cgctacgcag
     1081 ctcatctcca acatggacat tgacgtgatc ctaggtggag gccgaaagta catgtttccc
     1141 atgggaaccc cagaccctga gtacccagat gactacagcc aaggtgggac caggctggac
     1201 gggaagaatc tggtgcagga atggctggcg aagcgccagg gtgcccggta tgtgtggaac
     1261 cgcactgagc tcatgcaggc ttccctggac ccgtctgtga cccatctcat gggtctcttt
     1321 gagcctggag acatgaaata cgagatccac cgagactcca cactggaccc ctccctgatg
     1381 gagatgacag aggctgccct gcgcctgctg agcaggaacc cccgcggctt cttcctcttc
     1441 gtggagggtg gtcgcatcga ccatggtcat catgaaagca gggcttaccg ggcactgact
     1501 gagacgatca tgttcgacga cgccattgag agggcgggcc agctcaccag cgaggaggac
     1561 acgctgagcc tcgtcactgc cgaccactcc cacgtcttct ccttcggagg ctaccccctg
     1621 cgagggagct ccatcttcgg gctggcccct ggcaaggccc gggacaggaa ggcctacacg
     1681 gtcctcctat acggaaacgg tccaggctat gtgctcaagg acggcgcccg gccggatgtt
     1741 accgagagcg agagcgggag ccccgagtat cggcagcagt cagcagtgcc cctggacgaa
     1801 gagacccacg caggcgagga cgtggcggtg ttcgcgcgcg gcccgcaggc gcacctggtt
     1861 cacggcgtgc aggagcagac cttcatagcg cacgtcatgg ccttcgccgc ctgcctggag
     1921 ccctacaccg cctgcgacct ggcgcccccc gccggcacca ccgacgccgc gcacccgggt
     1981 taagcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     2041 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     2101 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     2161 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     2221 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     2281 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     2341 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     2401 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     2461 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     2521 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     2581 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     2641 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     2701 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     2761 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     2821 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     2881 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     2941 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     3001 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     3061 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     3121 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     3181 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     3241 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     3301 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     3361 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     3421 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     3481 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     3541 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     3601 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     3661 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     3721 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     3781 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     3841 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     3901 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     3961 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     4021 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     4081 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     4141 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4201 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     4261 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     4321 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     4381 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     4441 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     4501 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     4561 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4621 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4681 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4741 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4801 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4861 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4921 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4981 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5041 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5101 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     5161 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt
     5221 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5281 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     5341 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     5401 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     5461 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     5521 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     5581 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     5641 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     5701 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     5761 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     5821 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     5881 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     5941 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6001 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     6061 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6121 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6181 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     6241 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     6301 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     6361 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     6421 tgccacctga cgtc
//