| 质粒名称: | pSL1363 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002434 |
| 产品用途: | Mammalian PhCMV-driven constitutive LIRP expression vector (PhCMV-LIRP-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | LIRP |
| 物种来源: | synthetic |
| 密码子优化: | 人源表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
|
pSL1363
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1363载体质粒基本信息
pSL1363质粒图谱载体图谱和pSL1363载体序列质粒序列多克隆位点信息
pSL1363质粒载体简介
pSL1363质粒序列载体序列
LOCUS pSL1363 6186 bp DNA circular SYN 05-MAR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6186)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6186
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 213..232
/label=oSCL771
misc_feature 232..819
/label=CMV
primer_bind 863..900
/label=oSCL2337
primer_bind 863..882
/label=T7
primer_bind complement(863..882)
/label=oSCL1121
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 883..905
/label=oSCL2210
primer_bind 883..900
/label=Oscl32-1
primer_bind 883..900
/label=Oscl42-1
regulatory 883..892
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
primer_bind 884..900
/label=oSCL1118
regulatory 884..892
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 892..1044
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRSQAYKVT
CSVRQS"
primer_bind complement(953..998)
/label=oSCL1156
primer_bind 979..999
/label=oSCL1157
primer_bind complement(986..1061)
/label=oSCL2215
misc_feature 1005^1006
/label=Feature 16
primer_bind 1006..1025
/label=oSCL2216
primer_bind 1025..1062
/label=oSCL2328
primer_bind 1025..1062
/label=oSCL2329
primer_bind 1025..1062
/label=oSCL2330
primer_bind complement(1025..1044)
/label=oSCL2327
primer_bind complement(1033..1061)
/label=oSCL2217
primer_bind 1044..1062
/label=oSCL1757
primer_bind complement(1044..1061)
/label=oSCL2219
misc_feature 1045..1455
/label=LOV2
primer_bind complement(1434..1455)
/label=oSCL1758
primer_bind 1436..1482
/label=oSCL2218
primer_bind 1436..1455
/label=oSCL2216
primer_bind 1436..1455
/label=oSCL2220
CDS 1456..1683
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="QKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATN
SDCELIVKAMQGLLKDGNPIPSAIAANSGIY"
misc_feature 1456
/label=Feature 17
primer_bind complement(1557..1578)
/label=oSCL2219
primer_bind 1578..1598
/label=oSCL2220
misc_feature 1578^1579
/label=Feature 18
primer_bind complement(1663..1706)
/label=oSCL2211
primer_bind complement(1663..1692)
/label=Oscl32-2
primer_bind complement(1663..1683)
/label=oSCL093
primer_bind complement(1663..1683)
/label=oSCL396
terminator 1783..2010
/label=bGH PA
polyA_signal 1786..2010
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2056..2484
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2498..2827
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2678..2813
/label=SV40 ori
/note="SV40 origin of replication"
misc_feature 2678..2755
/label=SV40 ORI
CDS 2894..3688
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3862..3983
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4028..4048)
/label=M13-rev
primer_bind complement(4032..4048)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(4054..4076)
/label=LacO
protein_bind 4056..4072
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4080..4110)
/label=lac promoter
/note="promoter for the E. coli lac operon"
promoter complement(4081..4110)
/label=lac
protein_bind 4125..4146
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4434..5019)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5190..6050)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6051..6155)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag gggccaccat ggcttcaaac
901 tttactcagt tcgtgctcgt ggacaatggt gggacagggg atgtgacagt ggctccttct
961 aatttcgcta atgggatcgc agagtggatc agctccaact cacggagcca ggcctacaag
1021 gtgacatgca gcgtcaggca gtctttggaa cgtatcgaaa agaattttgt catcacggac
1081 ccgcgtctgc ccgacaatcc gattatcttc gcgtcagact ctttcttaca actgactgag
1141 tatagtagag aggagatatt ggggcgtaac tgtagatttc ttcaggggcc agaaactgat
1201 cgggctaccg ttcgcaagat acgtgacgca atagacaacc agaccgaggt gacggtgcag
1261 ctgattaact acacaaagtc tgggaagaag ttctggaacc tgtttcattt acaacctatg
1321 agggaccaaa aaggtgacgt tcaatatttc atcggggttc agttagatgg gactgagcac
1381 gtgagagatg cagcagaaag agagggtgta atgcttatta aaaaaacagc cgagaatatc
1441 gacgaagccg ctggtcagaa gagaaagtat accatcaagg tggaggtccc caaagtggct
1501 acccagacag tgggcggagt cgaactgcct gtcgccgctt ggaggtccta cctgaacatg
1561 gagctcacta tcccaatttt cgctaccaat tctgactgtg aactcatcgt gaaggcaatg
1621 caggggctcc tcaaagacgg taatcctatc ccttccgcca tcgccgctaa ctcaggtatc
1681 tactaaggat ccactagtcc agtgtggtgg aattctgcag atatccagca cagtggcggc
1741 cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
1801 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
1861 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
1921 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
1981 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
2041 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
2101 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
2161 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
2221 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
2281 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
2341 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
2401 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
2461 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
2521 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
2581 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
2641 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
2701 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
2761 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
2821 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
2881 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
2941 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
3001 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
3061 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
3121 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
3181 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
3241 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
3301 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
3361 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
3421 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
3481 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
3541 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
3601 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
3661 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
3721 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
3781 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
3841 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
3901 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
3961 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
4021 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
4081 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
4141 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
4201 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
4261 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
4321 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
4381 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
4441 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
4501 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
4561 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
4621 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
4681 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
4741 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
4801 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
4861 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
4921 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt
4981 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
5041 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
5101 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
5161 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
5221 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
5281 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
5341 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
5401 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
5461 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
5521 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
5581 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
5641 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
5701 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
5761 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
5821 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
5881 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
5941 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
6001 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
6061 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
6121 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
6181 gacgtc
//
pSL1363载体图谱质粒图谱pdf版和pSL1363载体序列质粒序列等相关资料下载
pSL1363质粒载体应用举例
- 上一篇：pSL1343
- 下一篇：没有了
