| 质粒名称: | pSL516 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002417 |
| 产品用途: | Mammalian PhCMV-driven expression vector for MCP-specific SEAP-mRNA containing 16 tandem MS2-box repeats in the 3’-UTR (PhCMV-SEAP-(MS2-box)16-HHR-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | SEAP |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
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NA
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pSL516
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL516载体质粒基本信息
pSL516质粒图谱载体图谱和pSL516载体序列质粒序列多克隆位点信息
pSL516质粒载体简介
pSL516质粒序列载体序列
LOCUS pSL516 7806 bp DNA circular SYN 30-MAR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7806)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Aug 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7806
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
misc_feature 895..900
/label=NheI
primer_bind 897..932
/label=Oscl31-3
misc_feature 911..915
/label=New Feature
misc_feature 911..915
/label=New Feature
misc_feature 926..2485
/label=SEAP
primer_bind complement(2469..2504)
/label=oSCL083
primer_bind complement(2469..2485)
/label=Oscl31-4
polyA_signal 2477..2485
/label=SV40 late polyA
misc_feature 2517..2520
/label=linker
primer_bind complement(2522..2536)
/label=Oscl28-2
primer_bind complement(2523..2536)
/label=8x R
misc_feature 2537..2549
/label=linker
misc_feature 2555..2573
/label=MS2 box
misc_feature 2579..2588
/label=linker
misc_feature 2594..2612
/label=MS2 box
misc_feature 2618..2627
/label=linker
misc_feature 2618..2627
/label=linker
misc_feature 2626..2656
/label=hairpin
misc_feature 2632..2650
/label=MS2 box
misc_feature 2655..2667
/label=linker
misc_feature 2666..2696
/label=hairpin
misc_feature 2672..2690
/label=MS2 box
misc_feature 2700..2705
/label=linker
misc_feature 2714..2729
/label=linker
misc_feature 2735..2738
/label=linker
primer_bind complement(2740..2754)
/label=Oscl28-2
primer_bind complement(2741..2754)
/label=8x R
misc_feature 2755..2767
/label=linker
misc_feature 2773..2791
/label=MS2 box
misc_feature 2797..2806
/label=linker
misc_feature 2812..2830
/label=MS2 box
misc_feature 2836..2845
/label=linker
misc_feature 2836..2845
/label=linker
misc_feature 2844..2874
/label=hairpin
misc_feature 2850..2868
/label=MS2 box
misc_feature 2873..2885
/label=linker
misc_feature 2884..2914
/label=hairpin
misc_feature 2890..2908
/label=MS2 box
misc_feature 2918..2923
/label=linker
misc_feature 2932..2947
/label=linker
misc_feature 2974..2986
/label=linker
misc_feature 2992..3010
/label=MS2 box
misc_feature 3016..3025
/label=linker
misc_feature 3031..3049
/label=MS2 box
misc_feature 3055..3064
/label=linker
misc_feature 3055..3064
/label=linker
misc_feature 3063..3093
/label=hairpin
misc_feature 3069..3087
/label=MS2 box
misc_feature 3092..3104
/label=linker
misc_feature 3103..3133
/label=hairpin
misc_feature 3109..3127
/label=MS2 box
misc_feature 3137..3142
/label=linker
misc_feature 3151..3166
/label=linker
misc_feature 3172..3184
/label=linker
misc_feature 3190..3208
/label=MS2 box
misc_feature 3214..3223
/label=linker
misc_feature 3229..3247
/label=MS2 box
misc_feature 3253..3262
/label=linker
misc_feature 3253..3262
/label=linker
misc_feature 3261..3291
/label=hairpin
misc_feature 3267..3285
/label=MS2 box
misc_feature 3290..3302
/label=linker
misc_feature 3301..3331
/label=hairpin
misc_feature 3307..3325
/label=MS2 box
misc_feature 3335..3340
/label=linker
misc_feature 3349..3364
/label=linker
misc_feature 3371..3446
/label=HHR Env140_nutR_H6
misc_feature complement(3379..3387)
/label=Stem III
misc_feature 3390..3405
/label=Stem I
terminator 3414..3428
/label=nutR-boxB
misc_feature 3432..3440
/label=Stem III
terminator 3475..3702
/label=bGH PA
misc_feature 4298..4375
/label=SV40 ORI
CDS 4517..5308
/label=Kan/neoR
primer_bind complement(5648..5668)
/label=M13-rev
misc_binding complement(5674..5696)
/label=LacO
promoter complement(5701..5730)
/label=lac
CDS complement(6813..7472)
/label=AmpR
promoter complement(7712..7740)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttagcg ccaccatgct gctgctgctg ctgctgctgg gcctgaggct
961 acagctctcc ctgggcatca tcccagttga ggaggagaac ccggacttct ggaaccgcga
1021 ggcagccgag gccctgggtg ccgccaagaa gctgcagcct gcacagacag ccgccaagaa
1081 cctcatcatc ttcctgggcg atgggatggg ggtgtctacg gtgacagctg ccaggatcct
1141 aaaagggcag aagaaggaca aactggggcc tgagataccc ctggccatgg accgcttccc
1201 atatgtggct ctgtccaaga catacaatgt agacaaacat gtgccagaca gtggagccac
1261 agccacggcc tacctgtgcg gggtcaaggg caacttccag accattggct tgagtgcagc
1321 cgcccgcttt aaccagtgca acacgacacg cggcaacgag gtcatctccg tgatgaatcg
1381 ggccaagaaa gcagggaagt cagtgggagt ggtaaccacc acacgagtgc agcacgcctc
1441 gccagccggc acctacgccc acacggtgaa ccgcaactgg tactcggacg ccgacgtgcc
1501 tgcctcggcc cgccaggagg ggtgccagga catcgctacg cagctcatct ccaacatgga
1561 cattgacgtg atcctaggtg gaggccgaaa gtacatgttt cgcatgggaa ccccagaccc
1621 tgagtaccca gatgactaca gccaaggtgg gaccaggctg gacgggaaga atctggtgca
1681 ggaatggctg gcgaagcgcc agggtgcccg gtatgtgtgg aaccgcactg agctcatgca
1741 ggcttccctg gacccgtctg tgacccatct catgggtctc tttgagcctg gagacatgaa
1801 atacgagatc caccgagact ccacactgga cccctccctg atggagatga cagaggctgc
1861 cctgcgcctg ctgagcagga acccccgcgg cttcttcctc ttcgtggagg gtggtcgcat
1921 cgaccatggt catcatgaaa gcagggctta ccgggcactg actgagacga tcatgttcga
1981 cgacgccatt gagagggcgg gccagctcac cagcgaggag gacacgctga gcctcgtcac
2041 tgccgaccac tcccacgtct tctccttcgg aggctacccc ctgcgaggga gctccatctt
2101 cgggctggcc cctggcaagg cccgggacag gaaggcctac acggtcctcc tatacggaaa
2161 cggtccaggc tatgtgctca aggacggcgc ccggccggat gttaccgaga gcgagagcgg
2221 gagccccgag tatcggcagc agtcagcagt gcccctggac gaagagaccc acgcaggcga
2281 ggacgtggcg gtgttcgcgc gcggcccgca ggcgcacctg gttcacggcg tgcaggagca
2341 gaccttcata gcgcacgtca tggccttcgc cgcctgcctg gagccctaca ccgcctgcga
2401 cctggcgccc cccgccggca ccaccgacgc cgcgcacccg ggttactcta gagtcggggc
2461 ggccggccgc ttcgagcaga catgagcggc cgctcaatct cgagttatga ggatccaact
2521 tgaaccggta agaattgcaa caaacaaatc accaccgtga ggatcaccca cggtggtgaa
2581 agccttagcc tagccgtgag gatcacccac ggtaggcaca agaaattcta cccgtgagga
2641 tcacccacgg gtagaatcac aaagactaca gccgtgagga tcacccacgg ctgtagactc
2701 aaacttaaaa aggatatctt cagcaaactg atccaacttg aaccggtaag aattgcaaca
2761 aacaaatcac caccgtgagg atcacccacg gtggtgaaag ccttagccta gccgtgagga
2821 tcacccacgg taggcacaag aaattctacc cgtgaggatc acccacgggt agaatcacaa
2881 agactacagc cgtgaggatc acccacggct gtagactcaa acttaaaaag gatatcttca
2941 gcaaactgat cggagacgag tccgtctcaa attgcaacaa acaaatcacc accgtgagga
3001 tcacccacgg tggtgaaagc cttagcctag ccgtgaggat cacccacggt aggcacaaga
3061 aattctaccc gtgaggatca cccacgggta gaatcacaaa gactacagcc gtgaggatca
3121 cccacggctg tagactcaaa cttaaaaagg atatcttcag caaactgaat tgcaacaaac
3181 aaatcaccac cgtgaggatc acccacggtg gtgaaagcct tagcctagcc gtgaggatca
3241 cccacggtag gcacaagaaa ttctacccgt gaggatcacc cacgggtaga atcacaaaga
3301 ctacagccgt gaggatcacc cacggctgta gactcaaact taaaaaggat atcttcagca
3361 aactgaattc gtttaaaccc tcctcgtccg gggctgtgtc gccccgctga cgagccctga
3421 aaaagggcga aacgaggagg actagtgggc ccgtttaaac ccgctgatca gcctcgactg
3481 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
3541 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
3601 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
3661 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
3721 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
3781 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
3841 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
3901 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
3961 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
4021 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
4081 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
4141 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
4201 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
4261 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
4321 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
4381 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
4441 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
4501 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
4561 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
4621 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
4681 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
4741 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
4801 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
4861 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
4921 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
4981 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
5041 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
5101 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
5161 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
5221 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
5281 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
5341 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
5401 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
5461 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
5521 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
5581 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
5641 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
5701 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
5761 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
5821 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
5881 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
5941 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
6001 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
6061 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
6121 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
6181 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
6241 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
6301 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
6361 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
6421 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
6481 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
6541 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt
6601 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
6661 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
6721 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
6781 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
6841 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
6901 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
6961 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
7021 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
7081 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
7141 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
7201 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
7261 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
7321 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
7381 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
7441 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
7501 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
7561 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
7621 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
7681 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
7741 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
7801 gacgtc
//
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