pCMV-RT-La-EGFP

LOCUS       pCMV-RT-La-EGFP              7068 bp DNA     circular SYN 06-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        87..466
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     101..128
                     /label=OL1151
     primer_bind     complement(106..130)
                     /label=OL1047
     primer_bind     complement(168..195)
                     /label=OL1815
     primer_bind     318..355
                     /label=OL1892
     primer_bind     complement(369..388)
                     /label=OL1815
     primer_bind     388..407
                     /label=OL064
     promoter        467..670
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     569..588
                     /label=OL790
     primer_bind     615..636
                     /label=OL096
     primer_bind     621..641
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     621..640
                     /label=OL1335
     primer_bind     complement(622..639)
                     /label=OL400
     primer_bind     622..639
                     /label=OL443
     primer_bind     640..663
                     /label=OL152
     primer_bind     640..663
                     /label=OL159
     primer_bind     649..671
                     /label=OL084
     primer_bind     693..725
                     /label=OL2387
     primer_bind     712..731
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        712..730
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     712..730
                     /label=OL1124
     primer_bind     complement(727..746)
                     /label=OL1206
     primer_bind     complement(765..779)
                     /label=OL2538
     primer_bind     771..852
                     /label=OL1012
     primer_bind     complement(774..854)
                     /label=OL1013
     primer_bind     complement(778..824)
                     /label=OL2674
     primer_bind     complement(778..800)
                     /label=OL1036
     CDS             780..800
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     781..850
                     /label=OL1037
     primer_bind     complement(781..800)
                     /label=OL1298
     primer_bind     783..819
                     /label=OL4202
     CDS             800
                     /label=SGGSx2-sv40NLS-SGGSx2
                     /note="/created_by=chenp"
     primer_bind     801..846
                     /label=OL2673
     misc_feature    825..2855
                     /label=RT
     primer_bind     1265..1284
                     /label=OL4370
     primer_bind     complement(1437..1476)
                     /label=OL1894
     primer_bind     1437..1476
                     /label=OL4255
     primer_bind     1519..1533
                     /label=OL1416
     primer_bind     complement(1679..1697)
                     /label=OL1920
     primer_bind     complement(1708..1739)
                     /label=OL2389
     primer_bind     2168..2187
                     /label=OL4371
     primer_bind     complement(2295..2315)
                     /label=OL2669
     primer_bind     complement(2838..2877)
                     /label=OL4256
     primer_bind     2857..2882
                     /label=OL4282
     misc_feature    2863..2949
                     /label=Linker
     primer_bind     2863..2949
                     /label=OL4260
     primer_bind     complement(2863..2949)
                     /label=OL4261
     primer_bind     2932..2971
                     /label=OL4257
     primer_bind     complement(2935..2956)
                     /label=OL4283
     gene            2952..3533
                     /gene="SSB"
                     /label=SSB
                     /db_xref="GeneID:6741"
     CDS             2952..3533
                     /codon_start=1
                     /transl_table=11
                     /gene="SSB"
                     /product="Sjogren syndrome antigen B (autoantigen La)"
                     /label=La
                     /note="missing stop codon"
                     /db_xref="GeneID:6741"
                     /protein_id="ABM85385.1"
                     /translation="MAENGDNEKMAALEAKICHQIEYYFGDFNLPRDKFLKEQIKLDEG
                     WVPLEIMIKFNRLNRLTTDFNVIVEALSKSKAELMEISEDKTKIRRSPSKPLPEVTDEY
                     KNDVKNRSVYIKGFPTDATLDDIKEWLEDKGQVLNIQMRRTLHKAFKGSIFVVFDSIES
                     AKKFVETPGQKYKETDLLILFKDDYFAKKNE"
     primer_bind     complement(3516..3555)
                     /label=OL4258
     primer_bind     complement(3534..3558)
                     /label=OL2669
     primer_bind     3534..3555
                     /label=OL2671
     primer_bind     3536..3555
                     /label=OL4259
     primer_bind     3556..3577
                     /label=OL1302
     CDS             3579..3599
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3597..3635)
                     /label=OL4203
     CDS             3609..3635
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     primer_bind     3611..3660
                     /label=OL2670
     primer_bind     complement(3613..3635)
                     /label=OL2672
     primer_bind     complement(3621..3636)
                     /label=OL1303
     primer_bind     3635..3660
                     /label=OL899
     primer_bind     3635..3659
                     /label=PE001
     CDS             3636..3692
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     3636..3692
                     /label=OL866
     primer_bind     complement(3636..3692)
                     /label=OL867
     primer_bind     3636..3662
                     /label=OL2251
     primer_bind     3660..3713
                     /label=OL1687
     primer_bind     complement(3673..3711)
                     /label=OL308
     primer_bind     3674..3711
                     /label=OL309
     primer_bind     complement(3674..3700)
                     /label=PE002
     primer_bind     3684..3710
                     /label=PE003
     CDS             3693..4412
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     3693..3713
                     /label=OL1686
     primer_bind     3693..3713
                     /label=OL1704
     primer_bind     complement(3914..3933)
                     /label=OL957
     primer_bind     4346..4366
                     /label=OL697
     primer_bind     4356..4370
                     /label=OL1937
     primer_bind     complement(4387..4412)
                     /label=OL174
     primer_bind     complement(4388..4442)
                     /label=OL900
     primer_bind     complement(4389..4413)
                     /label=PE004
     primer_bind     complement(4389..4409)
                     /label=OL1833
     primer_bind     complement(4393..4432)
                     /label=OL1205
     primer_bind     complement(4393..4432)
                     /label=OL1215
     primer_bind     complement(4394..4435)
                     /label=OL1146
     primer_bind     complement(4395..4437)
                     /label=OL1038
     primer_bind     4409..4442
                     /label=OL1014
     primer_bind     complement(4410..4432)
                     /label=OL4203
     primer_bind     4412..4433
                     /label=OL1300
     primer_bind     complement(4412..4433)
                     /label=OL1303
     primer_bind     4413..4432
                     /label=OL1207
     primer_bind     4413..4427
                     /label=OL2537
     primer_bind     4418..4441
                     /label=OL1035
     CDS             4418..4435
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     4418..4435
                     /label=OL1133
     primer_bind     complement(4418..4435)
                     /label=OL1152
     primer_bind     complement(4458..4475)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4464..4688
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal
                     牛生长激素聚腺苷酸化信号
                     牛生长激素（BGH）多聚腺苷酸化信号能够保证高效的转录终止和mRNA的poly A结构"
     primer_bind     4464..4483
                     /label=OL1407
     primer_bind     complement(4469..4488)
                     /label=OL1410
     primer_bind     complement(4508..4525)
                     /label=OL698
     primer_bind     complement(4510..4529)
                     /label=OL1938
     primer_bind     complement(4669..4688)
                     /label=OL1684
     primer_bind     complement(4759..4775)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4759..4775)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4772..4794)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4783..4799
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4807..4837)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4852..4873
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4990..5007)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5161..5749)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5241..5260)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(5343..5362)
                     /label=OL1041
     primer_bind     complement(5476..5495)
                     /label=OL631
     primer_bind     5532..5551
                     /label=OL798
     primer_bind     5793..5812
                     /label=OL659
     CDS             complement(5920..6780)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6377..6396
                     /label=OL1922
     primer_bind     6543..6562
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(6543..6562)
                     /label=OL1923
     primer_bind     6696..6715
                     /label=OL1033
     primer_bind     complement(6696..6715)
                     /label=OL1034
     promoter        complement(6781..6885)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6964..6983)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6964..6983
                     /label=OL1919
ORIGIN
        1 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
       61 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
      121 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      181 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      241 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      301 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      361 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      421 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      481 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      541 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      601 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      661 aagcagagct ggtttagtga accgtcagat ccgctagaga tccgcggccg ctaatacgac
      721 tcactatagg gagagccgcc accatgaaac ggacagccga cggaagcgag ttcgagtcac
      781 caaagaagaa gcggaaagtc agcggaggct ctagcggcgg aagcaccctg aacattgaag
      841 acgagtatag actgcatgaa acaagcaagg aacccgacgt gtccctgggc tccacctggc
      901 tgtccgactt tccccaggcc tgggccgaga caggaggaat gggcctggcc gtgcggcagg
      961 cacccctgat catccctctg aaggccacct ctacacccgt gagcatcaag cagtacccta
     1021 tgtctcagga ggccagactg ggcatcaagc ctcacatcca gaggctgctg gaccagggca
     1081 tcctggtgcc atgccagagc ccctggaaca caccactgct gcccgtgaag aagccaggca
     1141 ccaatgacta tagacccgtg caggatctga gagaggtgaa caagagggtg gaggatatcc
     1201 accccaccgt gcccaaccct tacaatctgc tgtccggcct gcccccttct caccagtggt
     1261 atacagtgct ggacctgaag gatgccttct tttgtctgag actgcaccct accagccagc
     1321 cactgttcgc ctttgagtgg agggaccctg agatgggcat ctctggccag ctgacctgga
     1381 cacgcctgcc tcagggcttc aagaatagcc caacactgtt taacgaggcc ctgcaccgcg
     1441 acctggcaga tttccggatc cagcacccag atctgatcct gctgcagtac gtggacgatc
     1501 tgctgctggc cgccaccagc gagctggatt gccagcaggg aacacgcgcc ctgctgcaga
     1561 ccctgggaaa cctgggatat agggcatccg ccaagaaggc ccagatctgt cagaagcagg
     1621 tgaagtacct gggctatctg ctgaaggagg gccagagatg gctgacagag gccaggaagg
     1681 agacagtgat gggccagcca acacccaaga ccccaagaca gctgagggag ttcctgggca
     1741 aagcaggatt ttgcaggctg ttcatcccag gattcgcaga gatggcagca cctctgtacc
     1801 cactgaccaa gccgggcacc ctgtttaatt ggggccctga ccagcagaag gcctatcagg
     1861 agatcaagca ggccctgctg acagcaccag ccctgggcct gccagacctg accaagcctt
     1921 tcgagctgtt tgtggatgag aagcagggct acgccaaggg cgtgctgacc cagaagctgg
     1981 gaccatggag acggcccgtg gcctatctgt ccaagaagct ggacccagtg gcagcaggat
     2041 ggccaccatg cctgaggatg gtggcagcaa tcgccgtgct gacaaaggat gccggcaagc
     2101 tgaccatggg acagccactg gtcatcctgg caccacacgc agtggaggcc ctggtgaagc
     2161 agcctccaga tcgctggctg tctaacgccc ggatgacaca ctaccaggcc ctgctgctgg
     2221 acaccgatcg cgtgcagttt ggccctgtgg tggccctgaa tccagccacc ctgctgcctc
     2281 tgccagagga gggcctgcag cacaactgtc tggacatcct ggcagaggca cacggaacaa
     2341 ggccagacct gaccgatcag cccctgcctg acgccgatca cacatggtat accgatggaa
     2401 gctccctgct gcaggagggc cagaggaagg caggagcagc agtgaccaca gagacagaag
     2461 tgatctgggc caaggccctg ccagcaggca catccgccca gcgggccgag ctgatcgccc
     2521 tgacccaggc cctgaagatg gccgagggca agaagctgaa cgtgtacaca gactccagat
     2581 atgccttcgc caccgcacac atccacggag agatctacag gcgccggggc tggctgacct
     2641 ctgagggcaa ggagatcaag aacaaggatg agatcctggc cctgctgaag gccctgtttc
     2701 tgcccaagcg gctgagcatc atccactgtc ctggacacca gaagggacac tccgccgagg
     2761 caaggggcaa tcggatggcc gaccaggccg ccagaaaggc tgctattact gaaactcccg
     2821 acacttccac tctgctgatt gaaaactcct ccccttccgg aggatctagc ggaggctcct
     2881 ctggctctga gacacctggc acaagcgaga gcgcaacacc tgaaagcagc gggggcagca
     2941 gcggggggtc aatggctgaa aatggtgata atgaaaagat ggctgccctg gaggccaaaa
     3001 tctgtcatca aattgagtat tattttggcg acttcaattt gccacgggac aagtttctaa
     3061 aggaacagat aaaactggat gaaggctggg tacctttgga gataatgata aaattcaaca
     3121 ggttgaaccg tctaacaaca gactttaatg taattgtgga agcattgagc aaatccaagg
     3181 cagaactcat ggaaatcagt gaagataaaa ctaaaatcag aaggtctcca agcaaacccc
     3241 tacctgaagt gactgatgag tataaaaatg atgtaaaaaa cagatctgtt tatattaaag
     3301 gcttcccaac tgatgcaact cttgatgaca taaaagaatg gttagaagat aaaggtcaag
     3361 tactaaatat tcagatgaga agaacattgc ataaagcatt taagggatca atttttgttg
     3421 tgtttgatag cattgaatct gctaagaaat ttgtagagac ccctggccag aagtacaaag
     3481 aaacagacct gctaatactt ttcaaggacg attactttgc caaaaaaaat gaatctggcg
     3541 gctcaaaaag aaccgccgac ggcagcgaat tcgagtctcc caagaagaag aggaaagtcg
     3601 gctctggccc tgccgctaag agagtgaagc tggacgctac taacttcagc ctgctgaagc
     3661 aggctggaga cgtggaggag aaccctggac ctatggtgag caagggcgag gagctgttca
     3721 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3781 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     3841 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     3901 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     3961 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     4021 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     4081 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     4141 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     4201 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     4261 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     4321 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     4381 tcactctcgg catggacgag ctgtacaagt aaccggtcat catcaccatc accattgagt
     4441 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     4501 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     4561 tgagaaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     4621 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     4681 ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga cctctagcta
     4741 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4801 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag
     4861 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4921 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4981 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     5041 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5101 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5161 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5221 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     5281 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5341 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     5401 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5461 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5521 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5581 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     5641 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5701 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5761 gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag ggattttggt
     5821 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5881 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5941 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     6001 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6061 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6121 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6181 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6241 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     6301 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     6361 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     6421 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6481 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     6541 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     6601 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6661 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6721 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6781 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6841 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6901 agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag ggtctactct
     6961 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     7021 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggca
//