PCDH-TetO-mCherry-TRIM58-TurboID

LOCUS       PCDH-TetO-mCherry-TRIM58-TurboID              9654 bp DNA     circular SYN 04-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9654)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 13, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2472..3179
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    3180..3208
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    3216..4673
                     /label=TRIM58
     CDS             4680..4706
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4710..4736
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4740..4766
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4767..5747
                     /codon_start=1
                     /label=TurboID
                     /translation="ASKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKS
                     GDACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEKEFSRAD"
     misc_feature    5751..6338
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6222..6233)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6411..6644
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6716..6837
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6856..6991
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7029..7045)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7053..7069
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7077..7107)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7122..7143
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7431..8019)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8190..9050)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9051..9155)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9629..9645
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag aatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     2521 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     2581 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     2641 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     2701 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     2761 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     2821 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     2881 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     2941 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     3001 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     3061 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     3121 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
     3181 ccggactcag atctcgagct caagcttcgg aattcatggc ctgggcgccg cccggggagc
     3241 ggctgcgcga ggatgcgcgg tgcccggtgt gcctggattt cctgcaggag ccggtcagcg
     3301 tggactgcgg ccacagcttc tgcctcaggt gcatctccga gttctgcgag aagtcggacg
     3361 gcgcgcaggg cggcgtctac gcctgtccgc agtgccgggg ccccttccgg ccctcgggct
     3421 ttcgccccaa ccggcagctg gcgggcctgg tggagagcgt gcggcggctg gggttgggcg
     3481 cggggcccgg ggcgcggcga tgcgcgcggc acggcgagga cctgagccgc ttctgcgagg
     3541 aggacgaggc ggcgctgtgc tgggtgtgcg acgccggccc cgagcacagg acgcaccgca
     3601 cggcgccgct gcaggaggcc gccggcagct accaggtaaa gctccagatg gctctggaac
     3661 ttatgaggaa agagttggag gacgccttga ctcaggaggc caacgtgggg aaaaagactg
     3721 tcatttggaa ggagaaagtg gaaatgcaga ggcagcgctt cagattggag tttgagaagc
     3781 atcgtggctt tctggcccag gaggagcaac ggcagctgag gcggctggag gcggaggagc
     3841 gagcgacgct gcagagactg cgggagagca agagccggct ggtccagcag agcaaggccc
     3901 tgaaggagct ggcggatgag ctgcaggaga ggtgccagcg cccggccctg ggtctgctgg
     3961 agggtgtgag aggagtcctg agcagaagta aggctgtcac aaggctggaa gcagagaaca
     4021 tccccatgga actgaagaca gcatgctgca tccctgggag gagggagctc ttaaggaagt
     4081 tccaagtgga tgtaaagctg gatcccgcca cggcgcaccc gagtctgctc ttgaccgccg
     4141 acctgcgcag tgtgcaggat ggagaaccat ggagggatgt ccccaacaac cctgagcgat
     4201 ttgacacatg gccctgcatc ctgggtttgc agagcttctc atcagggagg cattactggg
     4261 aggttctggt gggagaagga gcagagtggg gtttaggggt ctgtcaagac acactgccaa
     4321 gaaaggggga aaccacgcca tctcctgaga atggggtctg ggccctgtgg ctgctgaaag
     4381 ggaatgagta catggtcctt gcctccccat cagtgcctct tctccaactg gaaagtcctc
     4441 gctgcattgg gattttcttg gactatgaag ccggtgaaat ttcattctac aatgtcacag
     4501 atggatctta tatctacaca ttcaaccaac tcttctctgg tcttcttcgg ccttactttt
     4561 tcatctgtga tgcaactcct cttatcttgc cacccacaac aatagcaggg tcaggaaatt
     4621 gggcatccag ggatcattta gatcctgctt ctgatgtaag agatgatcat ctcggatcct
     4681 acccgtatga tgttccggat tacgctggct atccctacga cgtgcccgac tatgccgggt
     4741 acccctatga cgtcccagac tacgcagcta gcaaagacaa tactgtgcct ctgaagctga
     4801 tcgctctcct ggctaatggc gagttccata gtggcgaaca gctgggagaa accctgggca
     4861 tgtccagggc cgctatcaac aagcacattc agactctgcg cgactggggc gtggacgtgt
     4921 tcaccgtgcc cggaaagggc tactctctgc ccgagcctat cccgctgctg aacgctaaac
     4981 agattctggg acagctggac ggcgggagcg tggcagtcct gcctgtggtc gactccacca
     5041 atcagtacct gctggatcga atcggcgagc tgaagagtgg ggatgcttgc attgcagaat
     5101 atcagcaggc agggagagga agcagaggga ggaaatggtt ctctcctttt ggagctaacc
     5161 tgtacctgag tatgttttgg cgcctgaagc ggggaccagc agcaatcggc ctgggcccgg
     5221 tcatcggaat tgtcatggca gaagcgctgc gaaagctggg agcagacaag gtgcgagtca
     5281 aatggcccaa tgacctgtat ctgcaggata gaaagctggc aggcatcctg gtggagctgg
     5341 ccggaataac aggcgatgct gcacagatcg tcattggcgc cgggattaac gtggctatga
     5401 ggcgcgtgga ggaaagcgtg gtcaatcagg gctggatcac actgcaggaa gcagggatta
     5461 acctggacag gaatactctg gccgctacgc tgatccgaga gctgcgggca gccctggaac
     5521 tgttcgagca ggaaggcctg gctccatatc tgccacggtg ggagaagctg gataacttca
     5581 tcaatagacc cgtgaagctg atcattgggg acaaagagat tttcgggatt agccggggga
     5641 ttgataaaca gggagccctg ctgctggaac aggacggagt tatcaaaccc tggatgggcg
     5701 gagaaatcag tctgcggtct gccgaaaagg aattcagcag ggccgactga aatcaacctc
     5761 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5821 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5881 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5941 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6001 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6061 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6121 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     6181 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6241 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc cgccttcgcc
     6301 ctcagacgag tcggatctcc ctttggccgc ctccccgccg gtacctttaa gaccaatgac
     6361 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6421 aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga
     6481 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6541 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6601 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     6661 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     6721 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6781 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6841 tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6901 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     6961 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg
     7021 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7081 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7141 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7201 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7261 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7321 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7381 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7441 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7501 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7561 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7621 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7681 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7741 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7801 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7861 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7921 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7981 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8041 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8101 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8161 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8221 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8281 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8341 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8401 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8461 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8521 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8581 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8641 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8701 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8761 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8821 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8881 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8941 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9001 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9061 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9121 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9181 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     9241 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     9301 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     9361 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     9421 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     9481 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     9541 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     9601 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//