Lenti-mEGFP-drG3BP1

LOCUS       Lenti-mEGFP-drG3BP1              10363 bp DNA     circular SYN 10-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 29, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2210..2513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2514..2717
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2756..2765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2762..3478
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3497..4930
                     /codon_start=1
                     /label=drG3BP1
                     /translation="MVMEKPSAQLVGREFVRQYYTLLNQAPDYLHRFYGKNSSYVHGGL
                     DNNGKPAEAVYGQSEIHKKVMALSFRDCHTKIRHVDAHATLNEGVVVQVLGGLSNNMQP
                     MRKFMQTFVLAPEGTVANKFYVHNDIFRYQDEVFGDSDSEPPEESEEDVEELERVHSPE
                     VVQEESSGYYEQTPCVEPEVPQEEVSVTPEPQPEPEVEVEPEPAAVELKAEPISQPEVH
                     VEEKTQRSPPSPTPADTAPTMPEDNRPSSWASVTSKNLPPGGVVPATGVPPHVVRVPSA
                     QPRVEVKTETQTTAQRPQRDQRPRDQRPGPSPAHRTPRPGVVREGESGESEVRRTVRYP
                     DSHQLFVGNVPHDVDKNELKEFFEQYGTVLELRINSGGKLPNFGFVVFDDSEPVQKILN
                     NRPIKLRGDVRLNVEEKKTRSAREGDRRDIRPRGPGGPRDRIGGSRGPPTRGGMAQKPS
                     FGAGRGTGPSEGRYTGPRQ"
     promoter        4941..5440
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5467..6261
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    6285..6873
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6756..6767)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7081..7714
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7843..7859)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7867..7883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7891..7921)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7936..7957
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8245..8830)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9001..9861)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9862..9966)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10014..10148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggc gttacataac
     2221 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     2281 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     2341 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     2401 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     2461 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     2521 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     2581 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     2641 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     2701 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac
     2761 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2821 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     2881 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2941 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     3001 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     3061 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3121 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3181 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     3241 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3301 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3361 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     3421 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     3481 ctcagatctc gagctcatgg ttatggaaaa gccgagtgcc cagctggttg gccgtgaatt
     3541 tgttcgtcag tattataccc tgctgaatca ggccccggat tatctgcatc gcttttatgg
     3601 caaaaatagc agctatgttc atggcggtct ggataataat ggcaaaccgg cagaagcagt
     3661 ttatggtcag agcgaaattc ataaaaaagt gatggccctg agttttcgtg attgccatac
     3721 caaaattcgc catgtggatg cacatgcaac cctgaatgaa ggcgttgtgg ttcaggtgct
     3781 gggcggtctg agtaataata tgcagccgat gcgtaaattc atgcagacct ttgtgctggc
     3841 cccggaaggc accgttgcca ataagtttta tgtgcataat gatatcttcc gctatcagga
     3901 tgaagttttt ggtgacagtg atagcgaacc gccggaagaa agtgaagaag atgttgaaga
     3961 actggaacgc gtgcatagcc cggaagtggt gcaggaagaa agcagcggct attatgaaca
     4021 gaccccgtgc gttgaaccgg aagttccgca ggaagaagtg agtgttaccc cggaaccgca
     4081 gccggaaccg gaagtggaag ttgaaccgga gccggcagca gttgaactga aagccgaacc
     4141 gattagccag ccggaagttc atgttgaaga aaaaacccag cgcagcccgc cgagcccgac
     4201 ccctgcagac acagctccga ccatgccgga agataatcgc ccgagcagct gggccagcgt
     4261 taccagcaaa aatctgccgc cgggcggtgt tgttccggca accggtgttc cgccgcatgt
     4321 tgttcgtgtg ccgagtgccc aaccgcgtgt tgaagttaaa accgaaaccc agaccaccgc
     4381 acagcgcccg cagcgtgatc agcgtccgcg tgatcagcgc ccgggtccta gccctgccca
     4441 tcgtaccccg cgtccgggtg ttgttcgcga aggtgaaagc ggcgaaagcg aagtgcgccg
     4501 taccgtgcgc tatccggata gtcatcagct gtttgttggt aatgttccgc atgatgtgga
     4561 taaaaatgaa ctgaaagaat ttttcgagca gtatggtacc gttctggaac tgcgcattaa
     4621 tagtggcggt aaactgccga attttggttt tgtggttttt gatgatagcg aacctgtgca
     4681 gaaaattctg aataatcgtc cgattaagct gcgtggtgac gttcgtctga atgtggaaga
     4741 aaagaaaacc cgcagtgccc gtgaaggtga ccgtcgcgat attcgtccgc gtggcccggg
     4801 tggtccgcgt gaccgtattg gtggcagtcg cggcccgccg acccgtggtg gtatggcaca
     4861 gaaaccgagt tttggtgcag gccgtggcac cggtccgagt gaaggtcgtt ataccggtcc
     4921 gcgccagtaa gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     4981 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     5041 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     5101 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     5161 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     5221 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     5281 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     5341 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     5401 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttcct
     5461 aggaccatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     5521 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     5581 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     5641 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     5701 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     5761 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     5821 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     5881 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     5941 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
     6001 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     6061 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     6121 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     6181 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     6241 cttcttgacg agttcttctg acccgggcgg ggcgcgtctg gaacaatcaa cctctggatt
     6301 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     6361 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     6421 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     6481 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     6541 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     6601 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6661 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     6721 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     6781 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     6841 cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct
     6901 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     6961 gctagaagca caagaggagg aggaggtggg tttttccagt cacacctcag gtacctttaa
     7021 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac
     7081 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     7141 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
     7201 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
     7261 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     7321 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
     7381 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
     7441 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
     7501 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
     7561 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7621 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7681 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     7741 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag
     7801 cgttttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     7861 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7921 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7981 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8041 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8101 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8161 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8221 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8281 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8341 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8401 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8461 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8521 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8581 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8641 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     8701 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8761 acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8821 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8881 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8941 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9001 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9061 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9961 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt
    10021 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    10081 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    10141 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
    10201 ctattaccac tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa
    10261 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttttt
    10321 aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//