| 质粒名称: | pAAVLINK-3'-TECTA-HA |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001712 |
| 产品用途: | 3' AAVLINK plasmid for TECTA expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TECTA |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-3'-TECTA-HA
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-3'-TECTA-HA载体质粒基本信息
pAAVLINK-3'-TECTA-HA质粒图谱载体图谱和pAAVLINK-3'-TECTA-HA载体序列质粒序列多克隆位点信息
pAAVLINK-3'-TECTA-HA质粒载体简介
pAAVLINK-3'-TECTA-HA质粒序列载体序列
LOCUS pAAVLINK-3'-TECTA-HA 6811 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6811)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 3, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6811
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
protein_bind 145..178
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..3529
/codon_start=1
/label=TECTA
/translation="GPLRDHSLQG**VLHGGRWPVLLPSPHRRLLHRLRLRPLPHL*WL
PL*LPDQLPTHPVHHRKQAKLRLFPQVCCHSQE*GPGPVTGLVG*AGGRDRVWLQHRDP
PSLQAHCAGQQ*TALSAPEAGAREDKYLFLWLPRGGGN*FWPEGCI*LEDLPVHHSPSE
HAEQHLWSVWPLQRQP***PGDAHGSACIECQ*VWAELGEEGHLLPGGLWGPLSVLCQG
GRFLQSAAAVQPDPQPERCLLQVSQQS*PHLLL*ELPV*LLHRWGRGADRLQLAAELRQ
HLPDSGDYGDWLEELHVLHCHLPSKQPLRELRECLPAPLRRHPPEE*LQPLLRGGLSLR
RWLRPQRQELHPAPQLRLLLRWQILRAQAAILEQRLHAALPLFPSQRDSVRPAPMQVRR
GVCAAQRGARLLQHQDLLLPGGRRRRLPHLRRRLPALPSQLRLRAVHHLPETARHLLPA
YHQLRQVVGPQPHHHFARLLLH*RRADSHQRPEHGQGEWHTSECSIYNWFGNQNLQQ*G
VSGD*HQPRHPDILQWFQRH*NQHQREAAEQSVRSLWQLQRGPNR*LCDLAREAGGKQR
GAGPELENQWHAEETSCPQLQRAAVLTVCSHV*QCAHPEDAG*WLLPEAHRHEGLLPAL
LWASRSPPILRVLLPGRLLQPQEVPAVRLPGRLRGGLPLLRDP*HRVD*EGELLRSG*R
SLCGGGLSQPNLRAGQWQGAVWLHRATPLWK*LT*HYRCRGDLQSSPNGSVHI*VQALP
ARF*EGGREDQ*QTVHRHRGGRFYLLSDQQHQRELWKHCAVQWHAYHV*KHTLDRKRQQ
HWQHHHQGPHDQCGIFMCL*AGYQDLLGFCCEAYAKCN*PDSSNPRRQLHHQDGSLQKR
LLQTSLPPG*SSVDDSRCAVCRGFCGWS*RHTFNPNTQQMLCHTHPR*Q**APIFHH*R
RVSEPQR*HHWHRGECSLPDLSLSRHRL*IHRGLRRSSPSLCSVTLRLRKVLL*NHLPT
QFQDCHRLHKRAQRTDHFSGTY*EKKAGLV*GQWRV*ADLHEPGGWASLQLCNRNPAGG
RQELQSL*FFNGTSSLDASSHHDPDFIMAFCL*IRHDL"
misc_feature 357..362
/label=SDS
misc_feature 1515..1520
/label=SDS
misc_feature 2883..2888
/label=SDS
misc_feature 3058..3063
/label=SDS
misc_feature 3408..3413
/label=SDS
CDS 3536..3562
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 3566..3592
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 3596..3622
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
terminator 3626..4057
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3770..3775
/label=SDS
repeat_region 4074..4214
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4074..4203
/label=AAV2 ITR
rep_origin 4289..4744
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5026..5130
/gene="bla"
/label=AmpR promoter
CDS 5131..5991
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6162..6750
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag ggtccactgc gagaccattc cctgcaagga
301 tgatgagtac tgcatggagg aaggtggcct gtactactgc caagcccgca ccgacgcctc
361 ctgcatcgtc tcaggctacg gccactacct cacctttgat ggcttcccct ttgacttcca
421 gaccagctgc ccactcatcc tgtgcaccac aggaagcagg ccaagctcag actctttccc
481 caagtttgtt gtcacagcca agaatgagga ccgggacccg tcactggcct tgtgggttaa
541 gcaggtggac gtgaccgtgt ttggctacag catcgtgatc caccgagctt acaagcacac
601 tgtgctggtc aacagtgaac ggctctatct gcccctgaag ctggggcaag ggaagataaa
661 tatcttttcc tttggcttcc acgtggtggt ggaaactgat tttggcctga aggttgtata
721 tgactggaag accttcctgt ccatcacagt ccctcggagc atgcagaaca gcacctatgg
781 tctgtgtggc cgctacaacg gcaaccctga tgatgacctg gagatgccca tgggtctgct
841 tgcatcgagt gtcaatgagt ttgggcagag ctgggtgaag agggacacct tctgccaggt
901 gggctgtggg gaccgctgtc cgtcctgtgc caaggtggaa ggtttctcca aagtgcagca
961 gctgtgcagc ctgatcccca accagaacgc tgccttctcc aagtgtcaca gcaaagttaa
1021 ccccaccttc ttctataaga actgcctgtt tgactcttgc atcgatgggg gcgcggtgca
1081 gaccgcctgc agctggctgc agaactacgc cagcacctgc cagactcagg ggattacggt
1141 gactggctgg aggaattaca cgtcctgcac tgtcacctgc cctccaaaca gccattacga
1201 gagctgcgtg agtgtctgcc agccccgctg cgccgccatc cgcctgaaga gtgactgcag
1261 ccactactgc gtggagggct gtcactgcga cgctggctac gtcctcaacg gcaagagctg
1321 catcctgccc cacagctgcg gctgctactc cgatggcaaa tattacgagc ccaagcagct
1381 attttggaac agcgactgca cgcggcgctg ccgctgtttc cgtcgcaacg tgattcagtg
1441 cgacccgcgc caatgcaagt cagacgagga gtgtgcgctg cgcaacgggg tgcgcggctg
1501 cttcagcacc aagacctcct actgcctggc ggccggcggc ggcgtcttcc gcaccttcga
1561 cggcgccttc ctgcgcttcc cagccaactg cgccttcgtg ctgtccacca tctgccagaa
1621 actgcccgac atctccttcc agcttatcat caacttcgac aagtggtcgg cccccaacct
1681 caccatcatt tcgcccgtct acttctacat taacgaagag cagattctca tcaacgaccg
1741 gaacacggtc aaggtgaatg gcacacaagt gaatgttcca tttataactg gtttggcaac
1801 caaaatctac agcagtgagg ggtttctggt gattgacacc agcccagaca tccagatata
1861 ctacaatggt ttcaacgtca ttaaaatcag catcagcgag aggctgcaga acaaagtgtg
1921 cggtctctgt ggcaacttca acggggacct aacagatgat tatgtgacct tgcgagggaa
1981 gccggtggta agcagcgtgg tgctggccca gagctggaaa accaatggca tgcagaagag
2041 acctcttgcc cccagctgca acgagctgca gttctcacag tatgcagcca tgtgtgacaa
2101 tgtgcacatc cagaagatgc agggtgatgg ctactgcctg aagctcaccg acatgaaggg
2161 cttcttccag ccctgctatg ggcttctcga tcccctccca ttctacgagt cctgctacct
2221 ggacggctgc tacaaccaca agaagttcca gctgtgcggc tccctggccg cctacgggga
2281 ggcctgccgc tccttcggga tccttagcac cgagtggatt gagaaggaga attgctcagg
2341 agtggttgaa gatccctgtg tgggggcgga ctgtcccaac cgaacttgcg agctgggcaa
2401 tggcagggag ctgtgtggct gcatcgagcc acccccctat ggaaataact cacatgacat
2461 tatcgatgca gaggtgacct gcaaagcagc ccaaatggaa gtgtccatat ctaagtgcaa
2521 gctcttccag ctcggttttg agagggaggg cgtgaggatc aatgacagac agtgcaccgg
2581 catcgagggg gaagatttta tctcctttca gatcaacaac accaaaggga attgtggaaa
2641 cattgtgcag tccaatggca cgcatatcat gtataaaaac acactctgga tcgaaagcgc
2701 caacaacact ggcaacatca tcaccaggga ccgcacgatc aatgtggaat tttcatgtgc
2761 ttatgagctg gatatcaaga tctccttgga ttctgttgtg aagcctatgc taagtgtaat
2821 taacctgaca gttccaaccc aagaaggcag cttcatcacc aagatggctc tctacaaaaa
2881 cgcctcctac aaacatcctt accgccaggg tgaagtagtg ttgacgactc gagatgtgct
2941 gtatgtaggg gtttttgtgg ttggagctga cgccacacat ttaatcctaa cactcaacaa
3001 atgctatgcc acacccaccc gagatagcaa tgataagctc cgatatttca tcattgaagg
3061 agggtgtcag aacctcaaag ataacaccat tggcatcgag gagaatgcag tctccctgac
3121 ctgtcgcttt cacgtcaccg tctttaaatt cataggggat tacgacgaag ttcaccttca
3181 ctgtgcagtg tcactctgcg actcagaaaa gtactcctgt aaaatcactt gcccacacaa
3241 ttccaggatt gccacagatt acacaaaaga gcccaaagaa cagatcattt cagtgggacc
3301 tattaggaga aaaaggctgg actggtgtga ggacaatgga gggtgtgagc agatttgcac
3361 gagccgggtg gatgggcctc tctgcagctg tgtaacagga accctgcagg aggacggcaa
3421 gagctgcaga gcctctaatt cttcaatgga acttcaagtc tggacgcttc ttctcatcat
3481 gatccagatt tcattatggc attttgtcta taaatcaggc acgacctcat gtacataccc
3541 gtatgatgtt ccggattacg ctggctatcc ctacgacgtg cccgactatg ccgggtaccc
3601 ctatgacgtc ccagactacg cataactcga gataatcaac ctctggatta caaaatttgt
3661 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
3721 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
3781 aaatcctggt tagttcttgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
3841 acaggggctc ggctgttggg cactgacaat tccgtggtgt ttatttgtga aatttgtgat
3901 gctattgctt tatttgtaac catctagctt tatttgtgaa atttgtgatg ctattgcttt
3961 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
4021 gtttcaggtt cagggggaga tgtgggaggt tttttaacgg accgagcggc cgcaggaacc
4081 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
4141 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
4201 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
4261 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
4321 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
4381 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
4441 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
4501 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
4561 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
4621 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
4681 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
4741 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
4801 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
4861 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
4921 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
4981 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
5041 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
5101 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
5161 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
5221 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
5281 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
5341 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
5401 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
5461 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
5521 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
5581 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
5641 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
5701 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
5761 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
5821 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
5881 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
5941 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
6001 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
6061 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
6121 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
6181 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
6241 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
6301 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
6361 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
6421 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
6481 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
6541 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
6601 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
6661 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
6721 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
6781 ctggcctttt gctggccttt tgctcacatg t
//
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