pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN9A-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN9A-FLAG             7280 bp DNA     circular SYN 25-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3993
                     /codon_start=1
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; 
                     NE-NA;NENA; PN1; SFNP"
                     /product="sodium channel protein type 9 subunit 
                     alphaisoform 2"
                     /label=SCN9A
                     /note="isoform 2 is encoded by transcript variant 2;sodium 
                     channel, voltage-gated, type IX, alpha polypeptide; sodium 
                     channel protein type 9 subunit alpha; sodium channel, 
                     voltage-gated, type IX, alpha subunit; voltage-gated sodium
                     channel alpha subunit Nav1.7; hNE-Na; peripheral sodium 
                     channel 1; neuroendocrine sodium channel; sodium channel 
                     protein type IX subunit alpha; voltage-gated sodium channel
                     subunit alpha Nav1.7"
                     /db_xref="CCDS:CCDS92892.1"
                     /db_xref="GeneID:6335"
                     /db_xref="HGNC:HGNC:10597"
                     /db_xref="MIM:603415"
                     /protein_id="NP_001352465.1"
                     /translation="KGKIWWNIRKTCYKIVEHSWFESFIVLMILLSSGALAFEDIYIER
                     KKTIKIILEYADKIFTYIFILEMLLKWIAYGYKTYFTNAWCWLDFLIVDVSLVTLVANT
                     LGYSDLGPIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFS
                     IMGVNLFAGKFYECINTTDGSRFPASQVPNRSECFALMNVSQNVRWKNLKVNFDNVGLG
                     YLSLLQVATFKGWTIIMYAAVDSVNVDKQPKYEYSLYMYIYFVVFIIFGSFFTLNLFIG
                     VIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKIQGCIFDLVT
                     NQAFDISIMVLICLNMVTMMVEKEGQSQHMTEVLYWINVVFIILFTGECVLKLISLRHY
                     YFTVGWNIFDFVVVIISIVGMFLADLIETYFVSPTLFRVIRLARIGRILRLVKGAKGIR
                     TLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEDGINDMFNFETFGNSMIC
                     LFQITTSAGWDGLLAPILNSKPPDCDPKKVHPGSSVEGDCGNPSVGIFYFVSYIIISFL
                     VVVNMYIAVILENFSVATEESTEPLSEDDFEMFYEVWEKFDPDATQFIEFSKLSDFAAA
                     LDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDSLRSQMEERF
                     MSANPSKVSYEPITTTLKRKQEDVSATVIQRAYRRYRLRQNVKNISSIYIKDGDRDDDL
                     LNKKDMAFDNVNENSSPEKTDATSSTTSPPSYDSVTKPDKEKYEQDRTEKEDKGKDSKE
                     SKK"
     misc_feature    2636..2641
                     /label=SDS
     misc_feature    2973..2978
                     /label=SDS
     misc_feature    3460..3465
                     /label=SDS
     CDS             4000..4065
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4069..4500
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4213..4218
                     /label=SDS
     repeat_region   4543..4683
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4543..4672
                     /label=AAV2 ITR
     repeat_region   4544..4684
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4758..5213
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5495..5599
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5600..6460
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6631..7219
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggaa aggaaaaatc
     1561 tggtggaaca tcaggaaaac ctgctacaag attgttgaac acagttggtt tgaaagcttc
     1621 attgtcctca tgatcctgct cagcagtggt gccctggctt ttgaagatat ttatattgaa
     1681 aggaaaaaga ccattaagat tatcctggag tatgcagaca agatcttcac ttacatcttc
     1741 attctggaaa tgcttctaaa atggatagca tatggttata aaacatattt caccaatgcc
     1801 tggtgttggc tggatttcct aattgttgat gtttctttgg ttactttagt ggcaaacact
     1861 cttggctact cagatcttgg ccccattaaa tcccttcgga cactgagagc tttaagacct
     1921 ctaagagcct tatctagatt tgaaggaatg agggtcgttg tgaatgcact cataggagca
     1981 attccttcca tcatgaatgt gctacttgtg tgtcttatat tctggctgat attcagcatc
     2041 atgggagtaa atttgtttgc tggcaagttc tatgagtgta ttaacaccac agatgggtca
     2101 cggtttcctg caagtcaagt tccaaatcgt tccgaatgtt ttgcccttat gaatgttagt
     2161 caaaatgtgc gatggaaaaa cctgaaagtg aactttgata atgtcggact tggttaccta
     2221 tctctgcttc aagttgcaac ttttaaggga tggacgatta ttatgtatgc agcagtggat
     2281 tctgttaatg tagacaagca gcccaaatat gaatatagcc tctacatgta tatttatttt
     2341 gtcgtcttta tcatctttgg gtcattcttc actttgaact tgttcattgg tgtcatcata
     2401 gataatttca accaacagaa aaagaagctt ggaggtcaag acatctttat gacagaagaa
     2461 cagaagaaat actataatgc aatgaaaaag ctggggtcca agaagccaca aaagccaatt
     2521 cctcgaccag ggaacaaaat ccaaggatgt atatttgacc tagtgacaaa tcaagccttt
     2581 gatattagta tcatggttct tatctgtctc aacatggtaa ccatgatggt agaaaaggag
     2641 ggtcaaagtc aacatatgac tgaagtttta tattggataa atgtggtttt tataatcctt
     2701 ttcactggag aatgtgtgct aaaactgatc tccctcagac actactactt cactgtagga
     2761 tggaatattt ttgattttgt ggttgtgatt atctccattg taggtatgtt tctagctgat
     2821 ttgattgaaa cgtattttgt gtcccctacc ctgttccgag tgatccgtct tgccaggatt
     2881 ggccgaatcc tacgtctagt caaaggagca aaggggatcc gcacgctgct ctttgctttg
     2941 atgatgtccc ttcctgcgtt gtttaacatc ggcctcctgc tcttcctggt catgttcatc
     3001 tacgccatct ttggaatgtc caactttgcc tatgttaaaa aggaagatgg aattaatgac
     3061 atgttcaatt ttgagacctt tggcaacagt atgatttgcc tgttccaaat tacaacctct
     3121 gctggctggg atggattgct agcacctatt cttaacagta agccacccga ctgtgaccca
     3181 aaaaaagttc atcctggaag ttcagttgaa ggagactgtg gtaacccatc tgttggaata
     3241 ttctactttg ttagttatat catcatatcc ttcctggttg tggtgaacat gtacattgca
     3301 gtcatactgg agaattttag tgttgccact gaagaaagta ctgaacctct gagtgaggat
     3361 gactttgaga tgttctatga ggtttgggag aagtttgatc ccgatgcgac ccagtttata
     3421 gagttctcta aactctctga ttttgcagct gccctggatc ctcctcttct catagcaaaa
     3481 cccaacaaag tccagctcat tgccatggat ctgcccatgg ttagtggtga ccggatccat
     3541 tgtcttgaca tcttatttgc ttttacaaag cgtgttttgg gtgagagtgg ggagatggat
     3601 tctcttcgtt cacagatgga agaaaggttc atgtctgcaa atccttccaa agtgtcctat
     3661 gaacccatca caaccacact aaaacggaaa caagaggatg tgtctgctac tgtcattcag
     3721 cgtgcttata gacgttaccg cttaaggcaa aatgtcaaaa atatatcaag tatatacata
     3781 aaagatggag acagagatga tgatttactc aataaaaaag atatggcttt tgataatgtt
     3841 aatgagaact caagtccaga aaaaacagat gccacttcat ccaccacctc tccaccttca
     3901 tatgatagtg taacaaagcc agacaaagag aaatatgaac aagacagaac agaaaaggaa
     3961 gacaaaggga aagacagcaa ggaaagcaaa aaatgtacag actacaaaga ccatgacggt
     4021 gattataaag atcatgacat cgactacaag gatgacgatg acaagtaact cgagataatc
     4081 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     4141 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     4201 ctttcatttt ctcctccttg tataaatcct ggttagttct tgccacggcg gaactcatcg
     4261 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     4321 tgtttatttg tgaaatttgt gatgctattg ctttatttgt aaccatctag ctttatttgt
     4381 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     4441 aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa
     4501 agatctggag accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
     4561 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     4621 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     4681 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     4741 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4801 tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt
     4861 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4921 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     4981 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5041 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg
     5101 ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct
     5161 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     5221 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     5281 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     5341 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     5401 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     5461 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5521 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5581 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5641 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5701 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5761 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5821 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5881 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5941 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6001 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6061 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     6121 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     6181 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6241 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6301 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6361 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6421 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6481 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6541 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6601 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6661 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6721 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     6781 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6841 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6901 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6961 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7021 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7081 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7141 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7201 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7261 ctggcctttt gctcacatgt
//